Mutagenetix > Incidental Mutation

Incidental Mutation 'R7044:Zfp600'

547202

Institutional Source

Beutler Lab

Gene Symbol

Zfp600

Ensembl Gene

ENSMUSG00000066007

Gene Name

zinc finger protein 600

Synonyms

Gm13164

MMRRC Submission

045143-MU

Accession Numbers

Essential gene?

Possibly non essential (E-score: 0.397) question?

Stock #

R7044 (G1)

Quality Score

225.009

Status

Not validated

Chromosome

Chromosomal Location

146093397-146135326 bp(+) (GRCm39)

Type of Mutation

nonsense

DNA Base Change (assembly)

C to T at 146131892 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Glutamine to Stop codon at position 187 (Q187*)

Ref Sequence

ENSEMBL: ENSMUSP00000126879 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000092750] [ENSMUST00000131932] [ENSMUST00000168483]

AlphaFold

A2A7V0

Predicted Effect

probably null
Transcript: ENSMUST00000092750
AA Change: Q187*

SMART Domains

Protein: ENSMUSP00000090425
Gene: ENSMUSG00000066007
AA Change: Q187*

Domain	Start	End	E-Value	Type
KRAB	28	90	1.29e-17	SMART
ZnF_C2H2	251	273	7.9e-4	SMART
ZnF_C2H2	279	301	7.9e-4	SMART
ZnF_C2H2	307	329	3.49e-5	SMART
ZnF_C2H2	335	357	1.95e-3	SMART
ZnF_C2H2	363	385	7.9e-4	SMART
ZnF_C2H2	391	413	7.9e-4	SMART
ZnF_C2H2	419	441	3.49e-5	SMART
ZnF_C2H2	447	469	3.89e-3	SMART
ZnF_C2H2	475	497	2.75e-3	SMART
ZnF_C2H2	503	525	3.04e-5	SMART
ZnF_C2H2	531	553	8.34e-3	SMART
ZnF_C2H2	559	581	5.67e-5	SMART
ZnF_C2H2	587	609	1.98e-4	SMART
ZnF_C2H2	615	637	5.29e-5	SMART
ZnF_C2H2	643	665	1.58e-3	SMART
ZnF_C2H2	671	693	1.28e-3	SMART
ZnF_C2H2	699	721	1.6e-4	SMART

Predicted Effect

probably benign
Transcript: ENSMUST00000131932

SMART Domains

Protein: ENSMUSP00000117892
Gene: ENSMUSG00000066007

Domain	Start	End	E-Value	Type
KRAB	28	90	1.29e-17	SMART

Predicted Effect

probably null
Transcript: ENSMUST00000168483
AA Change: Q187*

SMART Domains

Protein: ENSMUSP00000126879
Gene: ENSMUSG00000066007
AA Change: Q187*

Domain	Start	End	E-Value	Type
KRAB	28	90	1.29e-17	SMART
ZnF_C2H2	251	273	7.9e-4	SMART
ZnF_C2H2	279	301	7.9e-4	SMART
ZnF_C2H2	307	329	3.49e-5	SMART
ZnF_C2H2	335	357	1.95e-3	SMART
ZnF_C2H2	363	385	7.9e-4	SMART
ZnF_C2H2	391	413	7.9e-4	SMART
ZnF_C2H2	419	441	3.49e-5	SMART
ZnF_C2H2	447	469	3.89e-3	SMART
ZnF_C2H2	475	497	2.75e-3	SMART
ZnF_C2H2	503	525	3.04e-5	SMART
ZnF_C2H2	531	553	8.34e-3	SMART
ZnF_C2H2	559	581	5.67e-5	SMART
ZnF_C2H2	587	609	1.98e-4	SMART
ZnF_C2H2	615	637	5.29e-5	SMART
ZnF_C2H2	643	665	1.58e-3	SMART
ZnF_C2H2	671	693	1.28e-3	SMART
ZnF_C2H2	699	721	1.6e-4	SMART

Coding Region Coverage

1x: 100.0%
3x: 100.0%
10x: 99.7%
20x: 98.9%

Validation Efficiency

98% (40/41)

Allele List at MGI

Other mutations in this stock

Total: 42 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Ankrd6	T	A	4: 32,815,260 (GRCm39)	Q448L	possibly damaging	Het
Appl1	A	G	14: 26,650,634 (GRCm39)	S513P	possibly damaging	Het
Cdhr2	A	T	13: 54,881,134 (GRCm39)	K1066*	probably null	Het
Ckap5	T	C	2: 91,407,946 (GRCm39)	I824T	probably benign	Het
Cracdl	C	T	1: 37,651,361 (GRCm39)	D1169N	possibly damaging	Het
Ctbp2	T	C	7: 132,616,831 (GRCm39)	R35G	possibly damaging	Het
Dcdc2c	T	C	12: 28,520,493 (GRCm39)		probably benign	Het
Eya1	T	A	1: 14,301,634 (GRCm39)		probably null	Het
Fat4	G	C	3: 39,064,959 (GRCm39)	A4972P	probably benign	Het
Fat4	C	T	3: 39,064,960 (GRCm39)	A4972V	probably benign	Het
Fbxl8	T	A	8: 105,993,647 (GRCm39)	M1K	probably null	Het
Glyat	T	C	19: 12,627,629 (GRCm39)	S75P	probably benign	Het
Gm3543	T	A	14: 41,802,023 (GRCm39)	I154F	probably damaging	Het
Gm4565	T	A	7: 22,282,795 (GRCm39)	M8L	probably benign	Het
Gpr179	T	C	11: 97,240,616 (GRCm39)	Q269R	probably benign	Het
Hjv	C	A	3: 96,434,790 (GRCm39)	H176Q	possibly damaging	Het
Ikzf2	T	C	1: 69,578,060 (GRCm39)	H483R	possibly damaging	Het
Il25	G	A	14: 55,170,615 (GRCm39)	A63T	probably benign	Het
Krt27	T	A	11: 99,237,640 (GRCm39)	N366I	probably benign	Het
Mfge8	A	G	7: 78,792,268 (GRCm39)	V219A	probably benign	Het
Nrbp1	T	C	5: 31,407,290 (GRCm39)	V373A	probably damaging	Het
Or4c100	A	G	2: 88,356,830 (GRCm39)	N301S	probably benign	Het
Otulinl	A	G	15: 27,657,321 (GRCm39)		probably benign	Het
Pcdh20	A	G	14: 88,706,607 (GRCm39)	I231T	probably damaging	Het
Pfkp	A	G	13: 6,631,603 (GRCm39)	W151R	probably damaging	Het
Pikfyve	T	C	1: 65,286,013 (GRCm39)	I1084T	probably benign	Het
Poteg	T	C	8: 27,939,923 (GRCm39)	C27R	probably damaging	Het
Ptprz1	G	A	6: 23,044,345 (GRCm39)	V1266M	probably damaging	Het
Rpa1	T	C	11: 75,203,628 (GRCm39)	D358G	probably damaging	Het
Slx9	T	C	10: 77,350,226 (GRCm39)	D35G	possibly damaging	Het
Snapc4	A	G	2: 26,259,965 (GRCm39)	S495P	probably damaging	Het
Surf6	A	G	2: 26,789,368 (GRCm39)	I123T	probably damaging	Het
Sybu	A	T	15: 44,541,091 (GRCm39)	S324T	possibly damaging	Het
Trim3	A	T	7: 105,267,421 (GRCm39)	S319R	probably damaging	Het
Trmt11	A	G	10: 30,466,930 (GRCm39)	F72L	probably benign	Het
Tsks	C	T	7: 44,593,216 (GRCm39)	R61W	probably damaging	Het
Ube2k	T	C	5: 65,738,772 (GRCm39)	V36A	possibly damaging	Het
Ube3a	T	C	7: 58,938,161 (GRCm39)	V688A	probably damaging	Het
Vegfc	A	G	8: 54,610,080 (GRCm39)	K79E	possibly damaging	Het
Vmn1r77	G	A	7: 11,775,761 (GRCm39)	R179Q	probably benign	Het
Vmn2r97	T	A	17: 19,134,629 (GRCm39)	F16I	probably benign	Het
Zfp451	A	T	1: 33,841,248 (GRCm39)		probably benign	Het

Other mutations in Zfp600

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
PIT4131001:Zfp600	UTSW	4	146,131,802 (GRCm39)	frame shift	probably null
R1004:Zfp600	UTSW	4	146,133,103 (GRCm39)	unclassified	probably benign
R1323:Zfp600	UTSW	4	146,133,261 (GRCm39)	missense	probably damaging	1.00
R1323:Zfp600	UTSW	4	146,133,261 (GRCm39)	missense	probably damaging	1.00
R1702:Zfp600	UTSW	4	146,133,497 (GRCm39)	missense	probably benign	0.04
R2165:Zfp600	UTSW	4	146,133,488 (GRCm39)	nonsense	probably null
R5238:Zfp600	UTSW	4	146,131,741 (GRCm39)	splice site	probably null
R5548:Zfp600	UTSW	4	146,133,019 (GRCm39)	missense	possibly damaging	0.86
R5646:Zfp600	UTSW	4	146,131,670 (GRCm39)	missense	probably damaging	1.00
R5948:Zfp600	UTSW	4	146,131,645 (GRCm39)	missense	probably damaging	0.96
R6112:Zfp600	UTSW	4	146,131,701 (GRCm39)	missense	probably benign	0.41
R7836:Zfp600	UTSW	4	146,133,523 (GRCm39)	missense	probably benign
R8080:Zfp600	UTSW	4	146,133,182 (GRCm39)	missense	unknown
R8749:Zfp600	UTSW	4	146,133,151 (GRCm39)	missense	unknown
R9255:Zfp600	UTSW	4	146,131,673 (GRCm39)	missense	possibly damaging	0.70
Z1176:Zfp600	UTSW	4	146,133,209 (GRCm39)	missense	unknown
Z1177:Zfp600	UTSW	4	146,133,333 (GRCm39)	missense	probably damaging	1.00

Predicted Primers

PCR Primer
(F):5'- GAGATGCCACTCTCCAATCC -3'
(R):5'- TGTCGCATTCACTACATTTGTAAGG -3'

Sequencing Primer
(F):5'- GATGCCACTCTCCAATCCTCAAAC -3'
(R):5'- CATTTGTAAGGTTTCTCTCCTGAATG -3'

Posted On

2019-05-13