Incidental Mutation 'R7053:Prdm15'
| ID |
547742 |
| Institutional Source |
Beutler Lab
|
| Gene Symbol |
Prdm15
|
| Ensembl Gene |
ENSMUSG00000014039 |
| Gene Name |
PR domain containing 15 |
| Synonyms |
Zfp298, E130018M06Rik |
| MMRRC Submission |
045150-MU
|
| Accession Numbers |
|
| Essential gene? |
Non essential
(E-score: 0.000)
|
| Stock # |
R7053 (G1)
|
| Quality Score |
225.009 |
|
Status
|
Not validated
|
| Chromosome |
16 |
| Chromosomal Location |
97592667-97653050 bp(-) (GRCm39) |
| Type of Mutation |
nonsense |
| DNA Base Change (assembly) |
G to A
at 97595742 bp (GRCm39)
|
| Zygosity |
Heterozygous |
| Amino Acid Change |
Glutamine to Stop codon
at position 1029
(Q1029*)
|
| Ref Sequence |
ENSEMBL: ENSMUSP00000113791
(fasta)
|
| Gene Model |
predicted gene model for transcript(s):
[ENSMUST00000095849]
[ENSMUST00000121584]
[ENSMUST00000142295]
|
| AlphaFold |
E9Q8T2 |
| Predicted Effect |
probably null
Transcript: ENSMUST00000095849
AA Change: Q1055*
|
| SMART Domains |
Protein: ENSMUSP00000093533
Gene: ENSMUSG00000014039
AA Change: Q1055*
| Domain | Start | End | E-Value | Type |
|---|
|
SET
|
75 |
191 |
5.96e-1 |
SMART |
|
ZnF_C2H2
|
223 |
245 |
3.99e0 |
SMART |
|
low complexity region
|
290 |
303 |
N/A |
INTRINSIC |
|
ZnF_C2H2
|
402 |
424 |
3.89e-3 |
SMART |
|
ZnF_C2H2
|
434 |
457 |
2.75e-3 |
SMART |
|
ZnF_C2H2
|
468 |
488 |
1.88e2 |
SMART |
|
ZnF_C2H2
|
495 |
517 |
5.42e-2 |
SMART |
|
ZnF_C2H2
|
522 |
544 |
1.36e-2 |
SMART |
|
ZnF_C2H2
|
571 |
593 |
6.23e-2 |
SMART |
|
ZnF_C2H2
|
598 |
620 |
2.75e-3 |
SMART |
|
low complexity region
|
642 |
657 |
N/A |
INTRINSIC |
|
ZnF_C2H2
|
661 |
684 |
2.17e-1 |
SMART |
|
ZnF_C2H2
|
689 |
711 |
3.24e0 |
SMART |
|
ZnF_C2H2
|
725 |
747 |
1.38e-3 |
SMART |
|
ZnF_C2H2
|
753 |
775 |
5.67e-5 |
SMART |
|
ZnF_C2H2
|
781 |
803 |
3.11e-2 |
SMART |
|
ZnF_C2H2
|
809 |
831 |
8.34e-3 |
SMART |
|
ZnF_C2H2
|
837 |
859 |
4.79e-3 |
SMART |
|
ZnF_C2H2
|
865 |
888 |
4.79e-3 |
SMART |
|
ZnF_C2H2
|
894 |
917 |
5.06e-2 |
SMART |
|
low complexity region
|
948 |
959 |
N/A |
INTRINSIC |
|
low complexity region
|
1148 |
1170 |
N/A |
INTRINSIC |
|
| Predicted Effect |
probably null
Transcript: ENSMUST00000121584
AA Change: Q1029*
|
| SMART Domains |
Protein: ENSMUSP00000113791
Gene: ENSMUSG00000014039
AA Change: Q1029*
| Domain | Start | End | E-Value | Type |
|---|
|
SET
|
49 |
165 |
5.96e-1 |
SMART |
|
ZnF_C2H2
|
197 |
219 |
3.99e0 |
SMART |
|
low complexity region
|
264 |
277 |
N/A |
INTRINSIC |
|
ZnF_C2H2
|
376 |
398 |
3.89e-3 |
SMART |
|
ZnF_C2H2
|
408 |
431 |
2.75e-3 |
SMART |
|
ZnF_C2H2
|
442 |
462 |
1.88e2 |
SMART |
|
ZnF_C2H2
|
469 |
491 |
5.42e-2 |
SMART |
|
ZnF_C2H2
|
496 |
518 |
1.36e-2 |
SMART |
|
ZnF_C2H2
|
545 |
567 |
6.23e-2 |
SMART |
|
ZnF_C2H2
|
572 |
594 |
2.75e-3 |
SMART |
|
low complexity region
|
616 |
631 |
N/A |
INTRINSIC |
|
ZnF_C2H2
|
635 |
658 |
2.17e-1 |
SMART |
|
ZnF_C2H2
|
663 |
685 |
3.24e0 |
SMART |
|
ZnF_C2H2
|
699 |
721 |
1.38e-3 |
SMART |
|
ZnF_C2H2
|
727 |
749 |
5.67e-5 |
SMART |
|
ZnF_C2H2
|
755 |
777 |
3.11e-2 |
SMART |
|
ZnF_C2H2
|
783 |
805 |
8.34e-3 |
SMART |
|
ZnF_C2H2
|
811 |
833 |
4.79e-3 |
SMART |
|
ZnF_C2H2
|
839 |
862 |
4.79e-3 |
SMART |
|
ZnF_C2H2
|
868 |
891 |
5.06e-2 |
SMART |
|
low complexity region
|
922 |
933 |
N/A |
INTRINSIC |
|
low complexity region
|
1122 |
1144 |
N/A |
INTRINSIC |
|
| Predicted Effect |
probably benign
Transcript: ENSMUST00000142295
|
| SMART Domains |
Protein: ENSMUSP00000120497
Gene: ENSMUSG00000014039
| Domain | Start | End | E-Value | Type |
|---|
|
SET
|
49 |
165 |
5.96e-1 |
SMART |
|
low complexity region
|
230 |
243 |
N/A |
INTRINSIC |
|
ZnF_C2H2
|
342 |
364 |
3.89e-3 |
SMART |
|
ZnF_C2H2
|
369 |
392 |
2.75e-3 |
SMART |
|
ZnF_C2H2
|
403 |
423 |
1.88e2 |
SMART |
|
| Coding Region Coverage |
- 1x: 100.0%
- 3x: 100.0%
- 10x: 99.7%
- 20x: 99.1%
|
| Validation Efficiency |
|
| Allele List at MGI |
|
| Other mutations in this stock |
Total: 54 list
| Gene | Ref | Var | Chr/Loc | Mutation | Predicted Effect | Zygosity |
|---|
| Adam26a |
A |
T |
8: 44,021,836 (GRCm39) |
C551* |
probably null |
Het |
| Aldh1l1 |
C |
A |
6: 90,540,420 (GRCm39) |
T235K |
possibly damaging |
Het |
| Atp6v0a2 |
A |
G |
5: 124,783,923 (GRCm39) |
E257G |
probably damaging |
Het |
| AW146154 |
A |
G |
7: 41,131,988 (GRCm39) |
|
probably null |
Het |
| BC048507 |
T |
C |
13: 68,011,772 (GRCm39) |
Y50H |
probably benign |
Het |
| Brip1 |
T |
C |
11: 86,083,791 (GRCm39) |
N77D |
possibly damaging |
Het |
| Ccdc13 |
T |
C |
9: 121,662,904 (GRCm39) |
E37G |
probably damaging |
Het |
| Ccn6 |
A |
G |
10: 39,034,297 (GRCm39) |
Y102H |
probably damaging |
Het |
| Col27a1 |
T |
A |
4: 63,251,404 (GRCm39) |
|
probably benign |
Het |
| Corin |
T |
A |
5: 72,458,870 (GRCm39) |
I960L |
probably benign |
Het |
| Csnk2b |
A |
G |
17: 35,335,422 (GRCm39) |
|
probably benign |
Het |
| Cyp2d26 |
T |
C |
15: 82,676,801 (GRCm39) |
S182G |
probably benign |
Het |
| Dennd1a |
A |
G |
2: 37,851,666 (GRCm39) |
L74P |
probably damaging |
Het |
| Dip2c |
A |
G |
13: 9,660,740 (GRCm39) |
D838G |
probably damaging |
Het |
| Dkkl1 |
G |
T |
7: 44,857,022 (GRCm39) |
Q182K |
probably damaging |
Het |
| Dnai2 |
T |
C |
11: 114,629,521 (GRCm39) |
S183P |
probably damaging |
Het |
| Dnhd1 |
T |
C |
7: 105,344,161 (GRCm39) |
L1835P |
probably damaging |
Het |
| Espl1 |
T |
C |
15: 102,225,328 (GRCm39) |
|
probably null |
Het |
| Fam174a |
C |
T |
1: 95,252,953 (GRCm39) |
A185V |
probably damaging |
Het |
| Fhip1b |
C |
T |
7: 105,033,779 (GRCm39) |
G479D |
probably damaging |
Het |
| Gm40460 |
CACAGCCTCCCTTGCAGCCCCCACAGGAACTACAGCCTCCCTTGCAGCCCCCACAGGAACTACAGCCTCCCTTGCAGCCCCCACAGGAACTACAGCCTCCCTTGCAGCCCCCACAG |
CACAGCCTCCCTTGCAGCCCCCACAGGAACTACAGCCTCCCTTGCAGCCCCCACAGGAACTACAGCCTCCCTTGCAGCCCCCACAG |
7: 141,794,554 (GRCm39) |
|
probably benign |
Het |
| Grid2 |
G |
T |
6: 64,677,402 (GRCm39) |
A74S |
unknown |
Het |
| Gsdma3 |
T |
A |
11: 98,520,621 (GRCm39) |
M84K |
possibly damaging |
Het |
| Hlcs |
A |
T |
16: 94,068,874 (GRCm39) |
S262R |
possibly damaging |
Het |
| Lrp1 |
A |
G |
10: 127,376,963 (GRCm39) |
C4205R |
probably damaging |
Het |
| Mdc1 |
C |
T |
17: 36,157,218 (GRCm39) |
A181V |
probably benign |
Het |
| Mdga2 |
T |
A |
12: 66,736,158 (GRCm39) |
I357F |
probably benign |
Het |
| Mfn1 |
A |
G |
3: 32,586,114 (GRCm39) |
I21V |
probably benign |
Het |
| Mgat4b |
C |
T |
11: 50,124,367 (GRCm39) |
T409M |
probably damaging |
Het |
| Mgat4d |
G |
T |
8: 84,098,261 (GRCm39) |
K341N |
probably damaging |
Het |
| Mpo |
A |
T |
11: 87,694,336 (GRCm39) |
N109Y |
probably damaging |
Het |
| Mvp |
T |
C |
7: 126,586,776 (GRCm39) |
Q785R |
possibly damaging |
Het |
| Nim1k |
A |
G |
13: 120,189,145 (GRCm39) |
V88A |
probably damaging |
Het |
| Oasl2 |
A |
T |
5: 115,049,291 (GRCm39) |
I464L |
possibly damaging |
Het |
| Or5m11b |
G |
T |
2: 85,806,358 (GRCm39) |
C257F |
possibly damaging |
Het |
| Pkd1l2 |
A |
G |
8: 117,740,681 (GRCm39) |
F2139L |
probably damaging |
Het |
| Pop1 |
T |
G |
15: 34,530,421 (GRCm39) |
S940A |
probably benign |
Het |
| Ppp1r9a |
T |
A |
6: 4,905,670 (GRCm39) |
M75K |
probably damaging |
Het |
| Rffl |
T |
C |
11: 82,703,497 (GRCm39) |
K142R |
probably null |
Het |
| Rhou |
A |
G |
8: 124,380,934 (GRCm39) |
|
probably benign |
Het |
| Rp1l1 |
A |
T |
14: 64,268,958 (GRCm39) |
T1515S |
possibly damaging |
Het |
| Serpina1b |
T |
A |
12: 103,698,688 (GRCm39) |
S54C |
possibly damaging |
Het |
| Slc30a2 |
G |
A |
4: 134,074,726 (GRCm39) |
R161Q |
probably damaging |
Het |
| Taok3 |
T |
A |
5: 117,390,627 (GRCm39) |
D529E |
probably benign |
Het |
| Tmem18 |
T |
A |
12: 30,634,506 (GRCm39) |
M1K |
probably null |
Het |
| Tmem269 |
T |
A |
4: 119,066,464 (GRCm39) |
H198L |
probably damaging |
Het |
| Tnrc18 |
A |
G |
5: 142,772,984 (GRCm39) |
V432A |
unknown |
Het |
| Ttc6 |
A |
T |
12: 57,707,318 (GRCm39) |
T742S |
probably benign |
Het |
| Uhrf2 |
T |
A |
19: 30,069,519 (GRCm39) |
C749S |
probably damaging |
Het |
| Unc13c |
G |
A |
9: 73,839,579 (GRCm39) |
T424I |
probably damaging |
Het |
| Vmn2r111 |
T |
C |
17: 22,778,032 (GRCm39) |
N549S |
possibly damaging |
Het |
| Vmn2r61 |
A |
C |
7: 41,916,557 (GRCm39) |
D390A |
probably damaging |
Het |
| Xpo7 |
A |
G |
14: 70,922,298 (GRCm39) |
|
probably null |
Het |
| Zfp948 |
T |
A |
17: 21,805,121 (GRCm39) |
L37* |
probably null |
Het |
|
| Other mutations in Prdm15 |
| Allele | Source | Chr | Coord | Type | Predicted Effect | PPH Score |
|---|
|
IGL00973:Prdm15
|
APN |
16 |
97,607,367 (GRCm39) |
splice site |
probably benign |
|
|
IGL01325:Prdm15
|
APN |
16 |
97,607,717 (GRCm39) |
missense |
probably damaging |
1.00 |
|
IGL02195:Prdm15
|
APN |
16 |
97,637,029 (GRCm39) |
missense |
probably damaging |
1.00 |
|
IGL02473:Prdm15
|
APN |
16 |
97,638,805 (GRCm39) |
splice site |
probably null |
|
|
IGL02502:Prdm15
|
APN |
16 |
97,640,539 (GRCm39) |
missense |
probably damaging |
1.00 |
|
IGL02604:Prdm15
|
APN |
16 |
97,623,142 (GRCm39) |
missense |
probably benign |
|
|
R0408:Prdm15
|
UTSW |
16 |
97,636,986 (GRCm39) |
missense |
possibly damaging |
0.92 |
|
R0437:Prdm15
|
UTSW |
16 |
97,613,759 (GRCm39) |
missense |
probably benign |
0.00 |
|
R0497:Prdm15
|
UTSW |
16 |
97,595,534 (GRCm39) |
missense |
possibly damaging |
0.63 |
|
R0590:Prdm15
|
UTSW |
16 |
97,598,961 (GRCm39) |
missense |
possibly damaging |
0.95 |
|
R0630:Prdm15
|
UTSW |
16 |
97,638,907 (GRCm39) |
missense |
probably null |
1.00 |
|
R0661:Prdm15
|
UTSW |
16 |
97,630,882 (GRCm39) |
missense |
probably benign |
0.34 |
|
R0718:Prdm15
|
UTSW |
16 |
97,613,833 (GRCm39) |
missense |
possibly damaging |
0.89 |
|
R1144:Prdm15
|
UTSW |
16 |
97,609,908 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R1240:Prdm15
|
UTSW |
16 |
97,638,800 (GRCm39) |
missense |
probably damaging |
0.98 |
|
R1605:Prdm15
|
UTSW |
16 |
97,640,506 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R1908:Prdm15
|
UTSW |
16 |
97,638,885 (GRCm39) |
missense |
probably benign |
0.27 |
|
R2081:Prdm15
|
UTSW |
16 |
97,604,980 (GRCm39) |
nonsense |
probably null |
|
|
R2208:Prdm15
|
UTSW |
16 |
97,600,464 (GRCm39) |
splice site |
probably null |
|
|
R3787:Prdm15
|
UTSW |
16 |
97,598,945 (GRCm39) |
missense |
probably benign |
0.00 |
|
R3890:Prdm15
|
UTSW |
16 |
97,600,771 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R4326:Prdm15
|
UTSW |
16 |
97,607,715 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R4728:Prdm15
|
UTSW |
16 |
97,622,986 (GRCm39) |
missense |
probably benign |
0.04 |
|
R4952:Prdm15
|
UTSW |
16 |
97,607,277 (GRCm39) |
missense |
probably damaging |
0.99 |
|
R4998:Prdm15
|
UTSW |
16 |
97,595,689 (GRCm39) |
missense |
probably damaging |
0.97 |
|
R5225:Prdm15
|
UTSW |
16 |
97,609,875 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R5505:Prdm15
|
UTSW |
16 |
97,618,183 (GRCm39) |
missense |
possibly damaging |
0.76 |
|
R5628:Prdm15
|
UTSW |
16 |
97,600,823 (GRCm39) |
missense |
probably damaging |
0.98 |
|
R5721:Prdm15
|
UTSW |
16 |
97,608,296 (GRCm39) |
missense |
possibly damaging |
0.74 |
|
R5873:Prdm15
|
UTSW |
16 |
97,609,889 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R5980:Prdm15
|
UTSW |
16 |
97,613,770 (GRCm39) |
nonsense |
probably null |
|
|
R6311:Prdm15
|
UTSW |
16 |
97,600,255 (GRCm39) |
missense |
probably null |
0.08 |
|
R6540:Prdm15
|
UTSW |
16 |
97,637,005 (GRCm39) |
missense |
probably benign |
0.13 |
|
R7241:Prdm15
|
UTSW |
16 |
97,596,941 (GRCm39) |
missense |
possibly damaging |
0.50 |
|
R7468:Prdm15
|
UTSW |
16 |
97,636,842 (GRCm39) |
nonsense |
probably null |
|
|
R7473:Prdm15
|
UTSW |
16 |
97,623,046 (GRCm39) |
missense |
possibly damaging |
0.68 |
|
R7762:Prdm15
|
UTSW |
16 |
97,619,473 (GRCm39) |
missense |
probably benign |
0.00 |
|
R7911:Prdm15
|
UTSW |
16 |
97,613,792 (GRCm39) |
missense |
probably benign |
0.35 |
|
R8053:Prdm15
|
UTSW |
16 |
97,636,807 (GRCm39) |
missense |
probably benign |
0.17 |
|
R8127:Prdm15
|
UTSW |
16 |
97,638,910 (GRCm39) |
missense |
probably benign |
0.24 |
|
R8213:Prdm15
|
UTSW |
16 |
97,608,260 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R8708:Prdm15
|
UTSW |
16 |
97,618,066 (GRCm39) |
missense |
unknown |
|
|
R8768:Prdm15
|
UTSW |
16 |
97,638,888 (GRCm39) |
missense |
probably benign |
|
|
R9000:Prdm15
|
UTSW |
16 |
97,595,470 (GRCm39) |
missense |
probably benign |
0.03 |
|
R9513:Prdm15
|
UTSW |
16 |
97,607,704 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R9583:Prdm15
|
UTSW |
16 |
97,623,142 (GRCm39) |
missense |
probably benign |
|
|
RF002:Prdm15
|
UTSW |
16 |
97,600,829 (GRCm39) |
missense |
probably damaging |
1.00 |
|
RF021:Prdm15
|
UTSW |
16 |
97,609,956 (GRCm39) |
missense |
probably damaging |
1.00 |
|
Z1177:Prdm15
|
UTSW |
16 |
97,618,159 (GRCm39) |
missense |
possibly damaging |
0.54 |
|
| Predicted Primers |
PCR Primer
(F):5'- AAAGTGGGCCACTGACTGAG -3'
(R):5'- GCCTGAGCATGCAATTTTAGCAC -3'
Sequencing Primer
(F):5'- GCCACTGACTGAGGGTTTG -3'
(R):5'- TAGCACAGATTATGGCCTCTAGCG -3'
|
| Posted On |
2019-05-13 |
Incidental Mutation