Incidental Mutation 'R7057:Zscan4b'
ID547981
Institutional Source Beutler Lab
Gene Symbol Zscan4b
Ensembl Gene ENSMUSG00000095339
Gene Namezinc finger and SCAN domain containing 4B
SynonymsEG665780
MMRRC Submission
Accession Numbers
Is this an essential gene? Probably non essential (E-score: 0.088) question?
Stock #R7057 (G1)
Quality Score189.009
Status Not validated
Chromosome7
Chromosomal Location10900740-10905050 bp(-) (GRCm38)
Type of Mutationnonsense
DNA Base Change (assembly) A to T at 10901709 bp
ZygosityHeterozygous
Amino Acid Change Cysteine to Stop codon at position 202 (C202*)
Ref Sequence ENSEMBL: ENSMUSP00000148157 (fasta)
Gene Model predicted gene model for transcript(s): [ENSMUST00000168158] [ENSMUST00000210847]
Predicted Effect probably null
Transcript: ENSMUST00000168158
AA Change: C202*
SMART Domains Protein: ENSMUSP00000127301
Gene: ENSMUSG00000095339
AA Change: C202*

DomainStartEndE-ValueType
Pfam:SCAN 43 122 1.8e-17 PFAM
low complexity region 181 192 N/A INTRINSIC
ZnF_C2H2 394 416 2.75e-3 SMART
ZnF_C2H2 423 445 6.57e-1 SMART
ZnF_C2H2 451 473 7.26e-3 SMART
ZnF_C2H2 479 502 3.83e-2 SMART
Predicted Effect probably null
Transcript: ENSMUST00000210847
AA Change: C202*
Coding Region Coverage
  • 1x: 100.0%
  • 3x: 100.0%
  • 10x: 99.8%
  • 20x: 99.2%
Validation Efficiency
Allele List at MGI
Other mutations in this stock
Total: 67 list
GeneRefVarChr/LocMutationPredicted EffectZygosity
1810009J06Rik T A 6: 40,968,247 V220D probably benign Het
Ankrd1 T C 19: 36,118,233 E113G possibly damaging Het
Aqp12 T C 1: 93,011,996 L249P probably damaging Het
Atg4a-ps A G 3: 103,645,980 F15S possibly damaging Het
Bub1 T A 2: 127,829,527 M46L probably benign Het
C1ra G A 6: 124,517,725 E316K probably benign Het
C2cd4d A G 3: 94,363,493 H22R probably benign Het
Ccdc157 A G 11: 4,144,586 V480A probably benign Het
Cdc42ep3 G A 17: 79,335,523 probably benign Het
Cela1 T A 15: 100,682,893 T161S possibly damaging Het
Chtf18 G T 17: 25,721,126 A697E possibly damaging Het
Cr2 T C 1: 195,151,610 D957G possibly damaging Het
Cyp2c40 A G 19: 39,807,619 V60A probably damaging Het
Dazl CCATGATGGCGGC CC 17: 50,293,406 probably null Het
Dock2 A T 11: 34,227,684 L1824Q probably benign Het
Dock2 T C 11: 34,695,217 Y546C probably benign Het
Fam208a A T 14: 27,461,651 N689I probably damaging Het
Focad G A 4: 88,274,105 C557Y unknown Het
Ftmt T A 18: 52,332,108 N165K probably benign Het
Gen1 A C 12: 11,242,418 S457A probably benign Het
Gja1 T G 10: 56,388,033 S163A probably benign Het
Gm8267 T C 14: 44,722,024 I194M probably damaging Het
Golga3 T A 5: 110,188,663 S389R probably damaging Het
Gpc5 C A 14: 115,133,242 Q87K possibly damaging Het
Hmcn2 G A 2: 31,422,649 A3420T probably damaging Het
Htt A G 5: 34,821,723 S817G probably null Het
Hus1b C T 13: 30,947,550 C42Y possibly damaging Het
Iars2 A G 1: 185,289,367 F913L probably benign Het
Kcna4 A G 2: 107,295,320 E133G probably damaging Het
Klhl36 T C 8: 119,876,797 L597P probably benign Het
Ltv1 T C 10: 13,180,902 E299G possibly damaging Het
Mmp12 A G 9: 7,357,840 Y348C probably damaging Het
Mmp12 G A 9: 7,369,173 V270I probably benign Het
Mmp2 A G 8: 92,831,705 D134G probably damaging Het
Mrpl15 A T 1: 4,776,642 M237K probably benign Het
Ms4a6b G T 19: 11,526,889 V177F possibly damaging Het
Muc16 A G 9: 18,646,079 S2973P unknown Het
Olfr1219 A C 2: 89,074,464 I209S possibly damaging Het
Olfr1445 A T 19: 12,884,642 I254F probably damaging Het
Olfr1477 G T 19: 13,502,879 D179Y probably damaging Het
Olfr390 A C 11: 73,787,148 D70A possibly damaging Het
Olfr912 A T 9: 38,581,754 H159L probably damaging Het
Pikfyve T A 1: 65,247,205 I1201K probably benign Het
Plekhn1 T G 4: 156,233,917 M83L probably damaging Het
Pnlip G T 19: 58,676,263 D212Y probably damaging Het
Pomt2 T C 12: 87,127,378 N417S probably damaging Het
Pxylp1 T A 9: 96,825,050 M360L probably benign Het
Rbak G A 5: 143,173,927 T457I possibly damaging Het
Runx2 A T 17: 44,814,537 W31R probably null Het
Sec16a T A 2: 26,425,265 I1795F probably damaging Het
Sik2 A T 9: 50,998,561 I64N probably damaging Het
Slc26a8 T C 17: 28,638,397 E924G possibly damaging Het
Slc30a2 G A 4: 134,347,415 R161Q probably damaging Het
Slc45a4 C A 15: 73,587,638 D174Y probably damaging Het
Slc6a6 A C 6: 91,741,267 E354A probably damaging Het
Slc8a1 A T 17: 81,649,095 S171R probably damaging Het
Srgap1 T C 10: 121,804,953 I669M probably benign Het
Stk39 C A 2: 68,410,127 A87S possibly damaging Het
Tbx18 C A 9: 87,705,264 S600I possibly damaging Het
Tesc A C 5: 118,054,960 K114Q probably damaging Het
Tll1 T C 8: 64,101,881 D256G probably damaging Het
Tmem35b T A 4: 127,127,886 I45K probably benign Het
Tnks T C 8: 34,840,014 D1127G probably damaging Het
Trpm8 A T 1: 88,362,080 D920V probably null Het
U2af1 A T 17: 31,648,857 D79E probably benign Het
Zfp704 T C 3: 9,470,917 D331G probably damaging Het
Znrf3 G T 11: 5,282,442 P261Q probably benign Het
Other mutations in Zscan4b
AlleleSourceChrCoordTypePredicted EffectPPH Score
PIT4696001:Zscan4b UTSW 7 10902022 missense possibly damaging 0.77
R0628:Zscan4b UTSW 7 10901463 missense probably damaging 0.99
R2289:Zscan4b UTSW 7 10901862 critical splice donor site probably null
R3713:Zscan4b UTSW 7 10901891 missense probably benign 0.01
R6129:Zscan4b UTSW 7 10901888 missense probably benign 0.01
R6393:Zscan4b UTSW 7 10900901 missense possibly damaging 0.76
R7312:Zscan4b UTSW 7 10900940 missense probably benign 0.00
R7383:Zscan4b UTSW 7 10904033 missense possibly damaging 0.92
R7412:Zscan4b UTSW 7 10900864 missense probably damaging 1.00
R7412:Zscan4b UTSW 7 10901893 missense probably benign 0.03
R7449:Zscan4b UTSW 7 10904058 missense possibly damaging 0.95
Z1177:Zscan4b UTSW 7 10901737 missense possibly damaging 0.92
Predicted Primers PCR Primer
(F):5'- TAGCAATTGTTCTTGTCCTCAGAG -3'
(R):5'- GCACAGATGCCAGTAGACAC -3'

Sequencing Primer
(F):5'- GGATAAATCCATTGGTTGTTCCTC -3'
(R):5'- GACACCACACAAGATAGATTATTGG -3'
Posted On2019-05-13