Mutagenetix > Incidental Mutation

Incidental Mutation 'R7060:Vmn2r99'

548222

Institutional Source

Beutler Lab

Gene Symbol

Vmn2r99

Ensembl Gene

ENSMUSG00000090304

Gene Name

vomeronasal 2, receptor 99

Synonyms

EG665376

MMRRC Submission

045157-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.131) question?

Stock #

R7060 (G1)

Quality Score

225.009

Status

Validated

Chromosome

Chromosomal Location

19361949-19401098 bp(+) (GRCm38)

Type of Mutation

nonsense

DNA Base Change (assembly)

A to T at 19394564 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Arginine to Stop codon at position 849 (R849*)

Ref Sequence

ENSEMBL: ENSMUSP00000135236 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000176107] [ENSMUST00000231989]

AlphaFold

H3BK37

Predicted Effect

probably null
Transcript: ENSMUST00000176107
AA Change: R849*

SMART Domains

Protein: ENSMUSP00000135236
Gene: ENSMUSG00000090304
AA Change: R849*

Domain	Start	End	E-Value	Type
signal peptide	1	19	N/A	INTRINSIC
Pfam:ANF_receptor	81	448	5.7e-33	PFAM
Pfam:NCD3G	508	561	1.8e-21	PFAM
Pfam:7tm_3	593	829	4.6e-52	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000231989

Meta Mutation Damage Score

0.9756

Coding Region Coverage

1x: 100.0%
3x: 100.0%
10x: 99.7%
20x: 99.0%

Validation Efficiency

96% (78/81)

Allele List at MGI

Other mutations in this stock

Total: 79 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
2610044O15Rik8	A	C	8: 129,219,132 (GRCm38)	I404R	probably benign	Het
5730559C18Rik	T	C	1: 136,220,197 (GRCm38)	K339R	possibly damaging	Het
Abcg4	T	A	9: 44,275,128 (GRCm38)	T535S	probably benign	Het
Adamts19	A	T	18: 58,837,640 (GRCm38)	R99*	probably null	Het
Alg6	C	T	4: 99,761,961 (GRCm38)	L473F	possibly damaging	Het
Ankar	A	T	1: 72,656,113 (GRCm38)	N893K	probably benign	Het
Ankrd54	G	A	15: 79,055,539 (GRCm38)	A183V	possibly damaging	Het
Anpep	G	T	7: 79,841,794 (GRCm38)	T153K	probably benign	Het
Arap1	A	G	7: 101,409,357 (GRCm38)		probably null	Het
Aspg	A	T	12: 112,122,953 (GRCm38)	T392S	probably benign	Het
B230307C23Rik	A	C	16: 98,010,131 (GRCm38)	R68S	probably benign	Het
Bdp1	G	C	13: 100,059,494 (GRCm38)	N1253K	probably damaging	Het
Ccrl2	G	A	9: 111,055,614 (GRCm38)	S272F	probably damaging	Het
Cdon	T	C	9: 35,486,909 (GRCm38)	L974P	probably damaging	Het
Celsr1	C	A	15: 86,032,655 (GRCm38)	E372D	probably benign	Het
Cers1	A	T	8: 70,315,905 (GRCm38)	M16L	possibly damaging	Het
Col2a1	G	T	15: 97,976,141 (GRCm38)	Q1387K	unknown	Het
Ddx39b	G	A	17: 35,252,750 (GRCm38)	V291M	probably damaging	Het
Dppa2	T	A	16: 48,315,713 (GRCm38)	S143T	probably benign	Het
Dpy19l1	A	T	9: 24,423,123 (GRCm38)	M583K	possibly damaging	Het
Eno3	A	T	11: 70,661,419 (GRCm38)	D299V	possibly damaging	Het
Epha8	C	T	4: 136,931,158 (GRCm38)	V969M	probably damaging	Het
Fcgbp	A	T	7: 28,091,933 (GRCm38)	H873L	probably benign	Het
Fhad1	CGG	CG	4: 141,918,291 (GRCm38)		probably null	Het
Fntb	A	C	12: 76,887,875 (GRCm38)	N173T	possibly damaging	Het
Gins2	T	A	8: 120,582,141 (GRCm38)	M125L	probably benign	Het
Gys1	G	A	7: 45,440,013 (GRCm38)	A199T	probably damaging	Het
Herpud1	C	A	8: 94,390,763 (GRCm38)	H116N	probably benign	Het
Hoga1	C	A	19: 42,060,246 (GRCm38)	Y134*	probably null	Het
Il10ra	A	G	9: 45,256,224 (GRCm38)	I343T	probably benign	Het
Inpp5j	C	T	11: 3,500,133 (GRCm38)		probably null	Het
Itpkb	T	C	1: 180,333,130 (GRCm38)	S274P	probably damaging	Het
Kalrn	C	T	16: 34,357,048 (GRCm38)	C249Y	probably damaging	Het
Klhl35	G	C	7: 99,468,458 (GRCm38)	A70P	possibly damaging	Het
Lhx1	A	G	11: 84,520,282 (GRCm38)		probably null	Het
Lmbrd1	T	C	1: 24,692,966 (GRCm38)	V88A	probably benign	Het
Macc1	T	G	12: 119,447,455 (GRCm38)	L653V	probably damaging	Het
Madd	T	C	2: 91,177,107 (GRCm38)	D220G	probably damaging	Het
Mllt10	T	A	2: 18,159,560 (GRCm38)	H300Q	possibly damaging	Het
Mlxipl	G	A	5: 135,132,315 (GRCm38)	A363T	possibly damaging	Het
Mus81	G	T	19: 5,487,793 (GRCm38)	D78E	probably benign	Het
Mxd1	A	T	6: 86,653,159 (GRCm38)	L26M	probably damaging	Het
Nhsl1	C	T	10: 18,526,503 (GRCm38)	T1159M	probably damaging	Het
Nos1ap	A	G	1: 170,338,125 (GRCm38)	S190P	possibly damaging	Het
Nwd1	A	C	8: 72,666,694 (GRCm38)	D195A	probably damaging	Het
Olfr103	G	T	17: 37,336,461 (GRCm38)	T257N	probably benign	Het
Olfr1058	T	A	2: 86,386,225 (GRCm38)	R64S	possibly damaging	Het
Olfr424	A	T	1: 174,136,810 (GRCm38)	D22V	probably benign	Het
Olfr892-ps1	G	A	9: 38,190,096 (GRCm38)	V124I	probably damaging	Het
Otof	T	C	5: 30,388,356 (GRCm38)	D500G	possibly damaging	Het
Pcgf5	T	A	19: 36,442,939 (GRCm38)	Y190*	probably null	Het
Pdcd11	C	T	19: 47,110,979 (GRCm38)	T839I	probably benign	Het
Ppard	G	C	17: 28,298,912 (GRCm38)	S318T	probably benign	Het
Ppfia2	A	G	10: 106,762,109 (GRCm38)	K178E	probably damaging	Het
Ppm1n	G	T	7: 19,279,262 (GRCm38)	R255S	probably damaging	Het
Ppp1r21	A	G	17: 88,580,544 (GRCm38)	Y693C	probably damaging	Het
Ppp2r5d	A	T	17: 46,687,353 (GRCm38)	V169E	possibly damaging	Het
Prc1	A	T	7: 80,304,373 (GRCm38)	T53S	probably benign	Het
Pwp2	A	C	10: 78,173,250 (GRCm38)		probably null	Het
Rab5c	G	A	11: 100,719,963 (GRCm38)	R40C	probably damaging	Het
Ring1	A	G	17: 34,023,390 (GRCm38)	C48R	probably damaging	Het
Rpap1	T	C	2: 119,773,562 (GRCm38)	D496G	probably damaging	Het
Rspo4	C	A	2: 151,873,078 (GRCm38)	Q212K	unknown	Het
Samd9l	T	A	6: 3,372,716 (GRCm38)	D1515V	probably damaging	Het
Serinc3	T	C	2: 163,636,959 (GRCm38)	T83A	probably benign	Het
Setd5	A	C	6: 113,117,382 (GRCm38)	D420A	probably damaging	Het
Sidt2	T	C	9: 45,953,246 (GRCm38)	T62A	possibly damaging	Het
Smarcal1	T	C	1: 72,612,942 (GRCm38)	V621A	probably damaging	Het
Srcin1	A	G	11: 97,573,885 (GRCm38)	L12P	probably damaging	Het
Stx18	G	A	5: 38,121,255 (GRCm38)	D165N	possibly damaging	Het
Sumf2	A	G	5: 129,854,500 (GRCm38)	K139E	possibly damaging	Het
Tdo2	A	T	3: 81,969,559 (GRCm38)	I102N	probably damaging	Het
Tdp1	T	A	12: 99,911,688 (GRCm38)	S410T	probably benign	Het
Tmem94	A	T	11: 115,792,938 (GRCm38)	I726F	probably damaging	Het
Ttc21a	A	G	9: 119,966,676 (GRCm38)	E1192G	probably damaging	Het
Ttc8	T	C	12: 98,943,467 (GRCm38)	I52T	probably benign	Het
Wwc1	T	C	11: 35,915,176 (GRCm38)	K77E	possibly damaging	Het
Xirp2	A	T	2: 67,515,608 (GRCm38)	E2731V	probably damaging	Het
Zfp423	G	A	8: 87,782,879 (GRCm38)	T258I	probably damaging	Het

Other mutations in Vmn2r99

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00503:Vmn2r99	APN	17	19,378,854 (GRCm38)	missense	probably benign	0.01
IGL01113:Vmn2r99	APN	17	19,394,256 (GRCm38)	missense	probably benign	0.20
IGL01138:Vmn2r99	APN	17	19,382,623 (GRCm38)	missense	probably damaging	0.97
IGL01646:Vmn2r99	APN	17	19,393,658 (GRCm38)	splice site	probably benign
IGL01769:Vmn2r99	APN	17	19,380,115 (GRCm38)	missense	probably damaging	1.00
IGL02112:Vmn2r99	APN	17	19,380,232 (GRCm38)	missense	probably null	0.99
IGL02891:Vmn2r99	APN	17	19,378,690 (GRCm38)	nonsense	probably null
IGL03132:Vmn2r99	APN	17	19,378,223 (GRCm38)	nonsense	probably null
FR4548:Vmn2r99	UTSW	17	19,394,285 (GRCm38)	missense	probably damaging	0.97
FR4976:Vmn2r99	UTSW	17	19,394,285 (GRCm38)	missense	probably damaging	0.97
PIT4382001:Vmn2r99	UTSW	17	19,394,343 (GRCm38)	missense	probably damaging	1.00
R0196:Vmn2r99	UTSW	17	19,394,573 (GRCm38)	missense	probably benign	0.00
R0720:Vmn2r99	UTSW	17	19,379,043 (GRCm38)	missense	probably benign	0.00
R1501:Vmn2r99	UTSW	17	19,362,259 (GRCm38)	missense	possibly damaging	0.93
R1519:Vmn2r99	UTSW	17	19,380,060 (GRCm38)	missense	probably benign	0.00
R1670:Vmn2r99	UTSW	17	19,362,252 (GRCm38)	missense	probably benign	0.37
R1682:Vmn2r99	UTSW	17	19,377,945 (GRCm38)	missense	probably damaging	0.97
R1873:Vmn2r99	UTSW	17	19,362,153 (GRCm38)	missense	probably benign	0.25
R1967:Vmn2r99	UTSW	17	19,378,815 (GRCm38)	missense	probably benign	0.01
R2101:Vmn2r99	UTSW	17	19,377,991 (GRCm38)	missense	probably damaging	1.00
R2474:Vmn2r99	UTSW	17	19,378,629 (GRCm38)	missense	probably benign	0.04
R2519:Vmn2r99	UTSW	17	19,378,708 (GRCm38)	missense	probably damaging	0.99
R3911:Vmn2r99	UTSW	17	19,394,373 (GRCm38)	missense	possibly damaging	0.92
R3947:Vmn2r99	UTSW	17	19,378,990 (GRCm38)	missense	probably benign	0.40
R3949:Vmn2r99	UTSW	17	19,378,990 (GRCm38)	missense	probably benign	0.40
R4016:Vmn2r99	UTSW	17	19,378,570 (GRCm38)	missense	possibly damaging	0.86
R4413:Vmn2r99	UTSW	17	19,379,260 (GRCm38)	missense	probably damaging	1.00
R4594:Vmn2r99	UTSW	17	19,393,662 (GRCm38)	missense	probably damaging	1.00
R4999:Vmn2r99	UTSW	17	19,362,135 (GRCm38)	start codon destroyed	probably null	0.96
R5206:Vmn2r99	UTSW	17	19,378,606 (GRCm38)	missense	probably benign	0.40
R5362:Vmn2r99	UTSW	17	19,379,339 (GRCm38)	missense	probably benign	0.00
R5377:Vmn2r99	UTSW	17	19,379,269 (GRCm38)	missense	probably damaging	1.00
R5455:Vmn2r99	UTSW	17	19,394,146 (GRCm38)	nonsense	probably null
R6021:Vmn2r99	UTSW	17	19,377,948 (GRCm38)	missense	probably damaging	1.00
R6059:Vmn2r99	UTSW	17	19,378,980 (GRCm38)	missense	probably benign	0.00
R6214:Vmn2r99	UTSW	17	19,382,558 (GRCm38)	missense	probably benign	0.19
R6215:Vmn2r99	UTSW	17	19,382,558 (GRCm38)	missense	probably benign	0.19
R6313:Vmn2r99	UTSW	17	19,382,605 (GRCm38)	missense	probably damaging	1.00
R6646:Vmn2r99	UTSW	17	19,380,031 (GRCm38)	missense	probably damaging	1.00
R6810:Vmn2r99	UTSW	17	19,380,034 (GRCm38)	missense	probably benign	0.20
R6885:Vmn2r99	UTSW	17	19,380,195 (GRCm38)	missense	possibly damaging	0.52
R6991:Vmn2r99	UTSW	17	19,378,110 (GRCm38)	missense	probably benign	0.03
R7090:Vmn2r99	UTSW	17	19,393,710 (GRCm38)	missense	possibly damaging	0.83
R7094:Vmn2r99	UTSW	17	19,379,311 (GRCm38)	missense	probably benign	0.00
R7449:Vmn2r99	UTSW	17	19,379,145 (GRCm38)	missense	probably benign	0.01
R7789:Vmn2r99	UTSW	17	19,393,817 (GRCm38)	missense	possibly damaging	0.91
R8039:Vmn2r99	UTSW	17	19,380,040 (GRCm38)	missense	probably benign	0.00
R8493:Vmn2r99	UTSW	17	19,393,758 (GRCm38)	missense	probably benign	0.15
R8511:Vmn2r99	UTSW	17	19,394,181 (GRCm38)	missense	probably damaging	1.00
R8715:Vmn2r99	UTSW	17	19,393,660 (GRCm38)	critical splice acceptor site	probably benign
R9462:Vmn2r99	UTSW	17	19,378,126 (GRCm38)	nonsense	probably null
R9681:Vmn2r99	UTSW	17	19,378,627 (GRCm38)	missense	probably damaging	1.00
R9737:Vmn2r99	UTSW	17	19,362,301 (GRCm38)	missense	probably benign
Z1088:Vmn2r99	UTSW	17	19,379,301 (GRCm38)	missense	probably benign	0.18

Predicted Primers

PCR Primer
(F):5'- GTTCTTCTGTGTCTGGGTCACC -3'
(R):5'- TCCCATGTTGTTCATTTTCAAATATG -3'

Sequencing Primer
(F):5'- GTGTCTGGGTCACCTTTCTTC -3'
(R):5'- GCATCTATTGAGAAAGGAAGGAAAC -3'

Posted On

2019-05-13