Mutagenetix > Incidental Mutations

Incidental Mutation 'R7063:Gpr89'

548382

Institutional Source

Beutler Lab

Gene Symbol

Gpr89

Ensembl Gene

ENSMUSG00000028096

Gene Name

G protein-coupled receptor 89

Synonyms

4933412D19Rik, SH120

MMRRC Submission

Accession Numbers

Is this an essential gene?

Possibly non essential (E-score: 0.471) question?

Stock #

R7063 (G1)

Quality Score

225.009

Status

Validated

Chromosome

Chromosomal Location

96868281-96905346 bp(-) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

C to G at 96875698 bp

Zygosity

Heterozygous

Amino Acid Change

Arginine to Proline at position 312 (R312P)

Ref Sequence

ENSEMBL: ENSMUSP00000029738 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000029738] [ENSMUST00000058865] [ENSMUST00000107070]

Predicted Effect

probably damaging
Transcript: ENSMUST00000029738
AA Change: R312P

PolyPhen 2 Score 0.997 (Sensitivity: 0.41; Specificity: 0.98)

SMART Domains

Protein: ENSMUSP00000029738
Gene: ENSMUSG00000028096
AA Change: R312P

Domain	Start	End	E-Value	Type
transmembrane domain	4	26	N/A	INTRINSIC
transmembrane domain	38	60	N/A	INTRINSIC
transmembrane domain	75	97	N/A	INTRINSIC
transmembrane domain	110	127	N/A	INTRINSIC
Pfam:GPHR_N	140	207	1.1e-31	PFAM
Pfam:ABA_GPCR	276	446	4.9e-46	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000058865

SMART Domains

Protein: ENSMUSP00000058936
Gene: ENSMUSG00000038298

Domain	Start	End	E-Value	Type
PDZ	18	90	2.41e-17	SMART
PDZ	143	215	1e-14	SMART
PDZ	251	323	2.81e-18	SMART
PDZ	386	458	4.5e-17	SMART
low complexity region	505	514	N/A	INTRINSIC

Predicted Effect

probably benign
Transcript: ENSMUST00000107070

SMART Domains

Protein: ENSMUSP00000102685
Gene: ENSMUSG00000038298

Domain	Start	End	E-Value	Type
PDZ	18	90	2.41e-17	SMART
PDZ	143	215	1e-14	SMART
PDZ	251	323	2.81e-18	SMART
PDZ	386	458	4.5e-17	SMART
low complexity region	505	514	N/A	INTRINSIC

Coding Region Coverage

1x: 100.0%
3x: 99.9%
10x: 99.7%
20x: 99.0%

Validation Efficiency

100% (43/43)

Allele List at MGI

Other mutations in this stock

Total: 42 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Arhgap35	A	G	7: 16,565,113	V9A	probably benign	Het
Btbd6	A	G	12: 112,977,512	Y148C	probably damaging	Het
C130060K24Rik	A	G	6: 65,441,403		probably benign	Het
Casr	T	C	16: 36,494,574	I968V	probably benign	Het
Celf1	T	C	2: 91,012,844		probably null	Het
Chst2	G	T	9: 95,405,568	R242S	probably benign	Het
Clca3a1	T	A	3: 144,755,206	D228V	probably damaging	Het
Cwf19l2	A	G	9: 3,430,532	Y288C	probably benign	Het
Cyp2r1	A	G	7: 114,552,949	S49P	probably damaging	Het
Dnah5	A	G	15: 28,233,248	E251G	probably benign	Het
Eepd1	A	G	9: 25,483,036	R199G	possibly damaging	Het
Fat1	C	T	8: 45,040,775	T3986I	probably benign	Het
Gbp4	G	A	5: 105,118,448	R576C	probably damaging	Het
Glp1r	A	G	17: 30,925,558	Y235C	probably damaging	Het
Gm31371	G	A	8: 19,903,412	C7Y		Het
Gm6408	T	C	5: 146,483,784	I158T	probably benign	Het
Idh2	A	G	7: 80,095,684	V403A	probably damaging	Het
Lclat1	A	G	17: 73,239,991	E301G	possibly damaging	Het
Lgmn	A	G	12: 102,402,678	Y181H	probably damaging	Het
Lgr5	T	C	10: 115,456,734	Y417C	probably damaging	Het
Man1a	G	T	10: 54,030,744	N311K	probably damaging	Het
Nat10	G	A	2: 103,748,077	L228F	probably benign	Het
Olfr1417	A	G	19: 11,828,184	F281L	probably damaging	Het
Olfr1445	A	G	19: 12,884,085	D68G	probably damaging	Het
Olfr476	C	A	7: 107,968,204	A269E	probably benign	Het
Olfr958	T	G	9: 39,550,115	Y252S	possibly damaging	Het
Pcmtd2	T	A	2: 181,854,983	Y130*	probably null	Het
Rapgef5	A	G	12: 117,689,129	D62G	possibly damaging	Het
Serpina3m	A	T	12: 104,391,467	I217L	probably benign	Het
Supt16	T	C	14: 52,172,048	R802G	possibly damaging	Het
Sypl2	A	T	3: 108,217,655	M130K	probably benign	Het
Tmtc2	A	T	10: 105,348,525	L503Q	probably damaging	Het
Ubald2	A	G	11: 116,434,617	Q60R	probably benign	Het
Vav1	C	T	17: 57,311,860	Q673*	probably null	Het
Vgll2	T	C	10: 52,027,976	S312P	probably benign	Het
Vmn1r191	C	T	13: 22,178,694	A297T	probably benign	Het
Vmn1r7	T	A	6: 57,024,433	I281F	possibly damaging	Het
Vmn2r108	T	C	17: 20,481,148	Y30C	probably damaging	Het
Vmn2r16	A	G	5: 109,363,784	N619S	probably damaging	Het
Vstm5	A	G	9: 15,239,253		probably benign	Het
Zfp7	A	G	15: 76,891,719	I654V	possibly damaging	Het
Zmat2	A	G	18: 36,796,574	N129S	probably null	Het

Other mutations in Gpr89

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00421:Gpr89	APN	3	96898523	missense	probably damaging	1.00
IGL00757:Gpr89	APN	3	96871534	missense	probably benign	0.00
IGL01114:Gpr89	APN	3	96893549	missense	probably damaging	0.98
IGL02417:Gpr89	APN	3	96897425	nonsense	probably null
explorer	UTSW	3	96880069	splice site	probably null
R0207:Gpr89	UTSW	3	96871480	missense	probably damaging	0.99
R0650:Gpr89	UTSW	3	96897324	splice site	probably benign
R0704:Gpr89	UTSW	3	96880168	critical splice acceptor site	probably null
R1496:Gpr89	UTSW	3	96905210	missense	probably benign	0.00
R1869:Gpr89	UTSW	3	96875659	missense	probably benign	0.16
R1913:Gpr89	UTSW	3	96875633	missense	possibly damaging	0.91
R2264:Gpr89	UTSW	3	96872515	missense	probably damaging	0.99
R2276:Gpr89	UTSW	3	96897427	missense	probably damaging	1.00
R3822:Gpr89	UTSW	3	96892944	missense	probably benign	0.03
R3922:Gpr89	UTSW	3	96890899	missense	probably damaging	1.00
R4984:Gpr89	UTSW	3	96905196	missense	probably benign	0.02
R5761:Gpr89	UTSW	3	96892880	missense	probably damaging	1.00
R6185:Gpr89	UTSW	3	96890833	missense	probably damaging	0.99
R7164:Gpr89	UTSW	3	96871398	missense	probably benign	0.04
R7172:Gpr89	UTSW	3	96880069	splice site	probably null
R7215:Gpr89	UTSW	3	96880088	missense	probably damaging	1.00
R7536:Gpr89	UTSW	3	96890893	missense	probably damaging	0.96
R7708:Gpr89	UTSW	3	96880625	missense	possibly damaging	0.81
R7849:Gpr89	UTSW	3	96871490	nonsense	probably null
R8273:Gpr89	UTSW	3	96905189	missense	probably benign
RF019:Gpr89	UTSW	3	96905193	missense	probably benign

Predicted Primers

PCR Primer
(F):5'- ACAAATGGTCCCAAAGTTCAGG -3'
(R):5'- CATGGACTCATTTGCCTTTGTGAG -3'

Sequencing Primer
(F):5'- GTCCCAAAGTTCAGGAGTTAAAGCC -3'
(R):5'- TGTTCAGGCACATGTGACAC -3'

Posted On

2019-05-13