Incidental Mutation 'R7063:Chst2'
ID548401
Institutional Source Beutler Lab
Gene Symbol Chst2
Ensembl Gene ENSMUSG00000033350
Gene Namecarbohydrate sulfotransferase 2
SynonymsGn6st, GST-2, N-acetylglucosamine-6-O-sulfotransferase, C130041E03Rik
MMRRC Submission
Accession Numbers
Is this an essential gene? Probably non essential (E-score: 0.072) question?
Stock #R7063 (G1)
Quality Score225.009
Status Validated
Chromosome9
Chromosomal Location95399292-95406722 bp(-) (GRCm38)
Type of Mutationmissense
DNA Base Change (assembly) G to T at 95405568 bp
ZygosityHeterozygous
Amino Acid Change Arginine to Serine at position 242 (R242S)
Ref Sequence ENSEMBL: ENSMUSP00000040775 (fasta)
Gene Model predicted gene model for transcript(s): [ENSMUST00000036267]
Predicted Effect probably benign
Transcript: ENSMUST00000036267
AA Change: R242S

PolyPhen 2 Score 0.276 (Sensitivity: 0.91; Specificity: 0.88)
SMART Domains Protein: ENSMUSP00000040775
Gene: ENSMUSG00000033350
AA Change: R242S

DomainStartEndE-ValueType
low complexity region 6 45 N/A INTRINSIC
transmembrane domain 55 74 N/A INTRINSIC
low complexity region 88 114 N/A INTRINSIC
low complexity region 129 149 N/A INTRINSIC
Pfam:Sulfotransfer_1 164 507 7e-27 PFAM
Pfam:Sulfotransfer_3 165 446 6.6e-18 PFAM
Meta Mutation Damage Score 0.3386 question?
Coding Region Coverage
  • 1x: 100.0%
  • 3x: 99.9%
  • 10x: 99.7%
  • 20x: 99.0%
Validation Efficiency 100% (43/43)
MGI Phenotype FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] This locus encodes a sulfotransferase protein. The encoded enzyme catalyzes the sulfation of a nonreducing N-acetylglucosamine residue, and may play a role in biosynthesis of 6-sulfosialyl Lewis X antigen. [provided by RefSeq, Aug 2011]
PHENOTYPE: Mice homozygous for a knock-out allele display decreased lymphocyte binding to peripheral lymph node high endothelial venules, and significantly reduced lymphocyte homing to Peyer's patches, peripheral and mesenteric lymph nodes. [provided by MGI curators]
Allele List at MGI
Other mutations in this stock
Total: 42 list
GeneRefVarChr/LocMutationPredicted EffectZygosity
Arhgap35 A G 7: 16,565,113 V9A probably benign Het
Btbd6 A G 12: 112,977,512 Y148C probably damaging Het
C130060K24Rik A G 6: 65,441,403 probably benign Het
Casr T C 16: 36,494,574 I968V probably benign Het
Celf1 T C 2: 91,012,844 probably null Het
Clca3a1 T A 3: 144,755,206 D228V probably damaging Het
Cwf19l2 A G 9: 3,430,532 Y288C probably benign Het
Cyp2r1 A G 7: 114,552,949 S49P probably damaging Het
Dnah5 A G 15: 28,233,248 E251G probably benign Het
Eepd1 A G 9: 25,483,036 R199G possibly damaging Het
Fat1 C T 8: 45,040,775 T3986I probably benign Het
Gbp4 G A 5: 105,118,448 R576C probably damaging Het
Glp1r A G 17: 30,925,558 Y235C probably damaging Het
Gm31371 G A 8: 19,903,412 C7Y Het
Gm6408 T C 5: 146,483,784 I158T probably benign Het
Gpr89 C G 3: 96,875,698 R312P probably damaging Het
Idh2 A G 7: 80,095,684 V403A probably damaging Het
Lclat1 A G 17: 73,239,991 E301G possibly damaging Het
Lgmn A G 12: 102,402,678 Y181H probably damaging Het
Lgr5 T C 10: 115,456,734 Y417C probably damaging Het
Man1a G T 10: 54,030,744 N311K probably damaging Het
Nat10 G A 2: 103,748,077 L228F probably benign Het
Olfr1417 A G 19: 11,828,184 F281L probably damaging Het
Olfr1445 A G 19: 12,884,085 D68G probably damaging Het
Olfr476 C A 7: 107,968,204 A269E probably benign Het
Olfr958 T G 9: 39,550,115 Y252S possibly damaging Het
Pcmtd2 T A 2: 181,854,983 Y130* probably null Het
Rapgef5 A G 12: 117,689,129 D62G possibly damaging Het
Serpina3m A T 12: 104,391,467 I217L probably benign Het
Supt16 T C 14: 52,172,048 R802G possibly damaging Het
Sypl2 A T 3: 108,217,655 M130K probably benign Het
Tmtc2 A T 10: 105,348,525 L503Q probably damaging Het
Ubald2 A G 11: 116,434,617 Q60R probably benign Het
Vav1 C T 17: 57,311,860 Q673* probably null Het
Vgll2 T C 10: 52,027,976 S312P probably benign Het
Vmn1r191 C T 13: 22,178,694 A297T probably benign Het
Vmn1r7 T A 6: 57,024,433 I281F possibly damaging Het
Vmn2r108 T C 17: 20,481,148 Y30C probably damaging Het
Vmn2r16 A G 5: 109,363,784 N619S probably damaging Het
Vstm5 A G 9: 15,239,253 probably benign Het
Zfp7 A G 15: 76,891,719 I654V possibly damaging Het
Zmat2 A G 18: 36,796,574 N129S probably null Het
Other mutations in Chst2
AlleleSourceChrCoordTypePredicted EffectPPH Score
IGL02403:Chst2 APN 9 95405232 nonsense probably null
R1240:Chst2 UTSW 9 95405483 missense possibly damaging 0.68
R1537:Chst2 UTSW 9 95406141 missense probably benign 0.04
R1884:Chst2 UTSW 9 95405558 missense probably damaging 1.00
R2355:Chst2 UTSW 9 95406095 missense probably damaging 0.99
R4576:Chst2 UTSW 9 95405171 missense probably damaging 0.99
R4818:Chst2 UTSW 9 95405364 missense probably damaging 1.00
R4928:Chst2 UTSW 9 95406006 missense probably benign 0.10
R5366:Chst2 UTSW 9 95405465 missense probably damaging 1.00
R5703:Chst2 UTSW 9 95404932 missense probably damaging 0.99
R5902:Chst2 UTSW 9 95405609 missense probably damaging 1.00
R6339:Chst2 UTSW 9 95405750 missense probably damaging 1.00
R6752:Chst2 UTSW 9 95404749 nonsense probably null
Predicted Primers PCR Primer
(F):5'- GCTGGATCTTTAAGCAGCGG -3'
(R):5'- CTTCAACCAGAACCCTGAGG -3'

Sequencing Primer
(F):5'- TTGATAACCAGCGTGCGGTAC -3'
(R):5'- GAGGTGTTCTTCCTCTATGAGCC -3'
Posted On2019-05-13