Mutagenetix > Incidental Mutation

Incidental Mutation 'R7067:Vmn1r87'

548625

Institutional Source

Beutler Lab

Gene Symbol

Vmn1r87

Ensembl Gene

ENSMUSG00000070815

Gene Name

vomeronasal 1 receptor 87

Synonyms

V1rk1

MMRRC Submission

045163-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.049) question?

Stock #

R7067 (G1)

Quality Score

225.009

Status

Validated

Chromosome

Chromosomal Location

12865398-12866285 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

T to A at 12865849 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Glutamine to Leucine at position 146 (Q146L)

Ref Sequence

ENSEMBL: ENSMUSP00000154184 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000094827] [ENSMUST00000211249] [ENSMUST00000227443] [ENSMUST00000228800]

AlphaFold

Q8R255

Predicted Effect

probably benign
Transcript: ENSMUST00000094827
AA Change: Q146L

PolyPhen 2 Score 0.024 (Sensitivity: 0.95; Specificity: 0.81)

SMART Domains

Protein: ENSMUSP00000092422
Gene: ENSMUSG00000070815
AA Change: Q146L

Domain	Start	End	E-Value	Type
Pfam:TAS2R	1	285	1.2e-11	PFAM
Pfam:V1R	14	280	1.8e-36	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000211249
AA Change: Q146L

PolyPhen 2 Score 0.024 (Sensitivity: 0.95; Specificity: 0.81)

Predicted Effect

probably benign
Transcript: ENSMUST00000227443
AA Change: Q146L

PolyPhen 2 Score 0.024 (Sensitivity: 0.95; Specificity: 0.81)

Predicted Effect

probably benign
Transcript: ENSMUST00000228800
AA Change: Q146L

PolyPhen 2 Score 0.024 (Sensitivity: 0.95; Specificity: 0.81)

Meta Mutation Damage Score

0.0898

Coding Region Coverage

1x: 100.0%
3x: 99.9%
10x: 99.7%
20x: 99.1%

Validation Efficiency

98% (62/63)

Allele List at MGI

Other mutations in this stock

Total: 63 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
4932438H23Rik	T	A	16: 90,852,921 (GRCm39)	S72C	probably damaging	Het
Abca13	A	G	11: 9,241,845 (GRCm39)	D1236G	probably benign	Het
Abcc6	T	C	7: 45,668,114 (GRCm39)	N165D	probably benign	Het
Aebp1	A	G	11: 5,816,431 (GRCm39)		probably null	Het
Afg2a	C	T	3: 37,485,847 (GRCm39)	Q190*	probably null	Het
Anapc4	A	G	5: 53,019,577 (GRCm39)	T580A	probably benign	Het
Apob	T	C	12: 8,059,423 (GRCm39)	I2602T	probably damaging	Het
Arap2	A	G	5: 62,811,387 (GRCm39)		probably null	Het
Asap3	T	A	4: 135,968,673 (GRCm39)		probably null	Het
Bend4	A	G	5: 67,557,611 (GRCm39)	Y402H	probably damaging	Het
Ccn2	A	G	10: 24,472,873 (GRCm39)	Y261C	probably benign	Het
Crtc2	A	G	3: 90,167,489 (GRCm39)	N271S	probably benign	Het
Csf2rb2	A	T	15: 78,176,694 (GRCm39)	W233R	probably damaging	Het
Cyp2u1	G	A	3: 131,087,202 (GRCm39)	L460F	probably damaging	Het
Dnm3	A	G	1: 162,148,540 (GRCm39)	L277P	probably damaging	Het
Efemp1	A	T	11: 28,817,926 (GRCm39)	N135I	probably damaging	Het
Elapor2	G	T	5: 9,316,295 (GRCm39)	A9S	possibly damaging	Het
Fsip2	T	A	2: 82,811,078 (GRCm39)	S2466T	possibly damaging	Het
Gal3st2	C	T	1: 93,802,447 (GRCm39)	A276V	possibly damaging	Het
Gm47985	A	T	1: 151,059,241 (GRCm39)	D294V	unknown	Het
Golga1	A	T	2: 38,937,731 (GRCm39)	D104E	probably benign	Het
Hnrnpr	C	T	4: 136,054,704 (GRCm39)	A219V	probably damaging	Het
Hsd3b7	T	C	7: 127,399,888 (GRCm39)		probably null	Het
Hspa8	T	C	9: 40,715,921 (GRCm39)	I562T	probably damaging	Het
Htra4	C	T	8: 25,523,717 (GRCm39)	V283M	probably damaging	Het
Il36rn	C	T	2: 24,167,541 (GRCm39)	R11*	probably null	Het
Iqcd	A	G	5: 120,743,212 (GRCm39)	T325A	probably damaging	Het
Kif23	A	T	9: 61,832,271 (GRCm39)	M624K	probably benign	Het
Krt25	G	T	11: 99,208,209 (GRCm39)	Q340K	probably benign	Het
Lamp3	T	A	16: 19,518,413 (GRCm39)	N275Y	probably damaging	Het
Lpin2	A	C	17: 71,551,853 (GRCm39)	K789T	possibly damaging	Het
Mroh2b	T	C	15: 4,929,986 (GRCm39)	I24T	probably benign	Het
Muc16	T	A	9: 18,569,547 (GRCm39)	I991F	unknown	Het
Ntf5	T	A	7: 45,065,048 (GRCm39)	L60Q	probably damaging	Het
Nuak1	A	G	10: 84,276,158 (GRCm39)	S22P	possibly damaging	Het
Obox8	T	C	7: 14,066,979 (GRCm39)	T22A	possibly damaging	Het
Or8k21	T	A	2: 86,144,911 (GRCm39)	T240S	probably damaging	Het
Pde8a	T	C	7: 80,967,074 (GRCm39)	V405A	probably benign	Het
Phc2	T	C	4: 128,640,934 (GRCm39)	S147P	probably benign	Het
Pole	G	T	5: 110,482,084 (GRCm39)	G142V	probably damaging	Het
Poli	G	A	18: 70,642,488 (GRCm39)	Q508*	probably null	Het
Repin1	T	A	6: 48,574,850 (GRCm39)	L537*	probably null	Het
Rictor	C	T	15: 6,801,635 (GRCm39)	S441L	probably benign	Het
Samd7	G	C	3: 30,805,272 (GRCm39)	K18N	probably benign	Het
Slc30a2	T	A	4: 134,071,529 (GRCm39)		probably null	Het
Slit3	A	G	11: 35,399,057 (GRCm39)	S141G	probably benign	Het
Spon2	A	G	5: 33,371,958 (GRCm39)	S283P	probably damaging	Het
Srgap3	A	T	6: 112,734,266 (GRCm39)		probably benign	Het
Syde2	A	G	3: 145,694,019 (GRCm39)	D89G	probably benign	Het
Syne1	A	G	10: 5,184,586 (GRCm39)	I4099T	probably damaging	Het
Syt5	T	C	7: 4,546,075 (GRCm39)	D105G	probably benign	Het
Tlx3	C	T	11: 33,153,204 (GRCm39)	G86S	probably damaging	Het
Trim24	A	T	6: 37,934,775 (GRCm39)		probably null	Het
Umodl1	G	A	17: 31,201,246 (GRCm39)	V392I	probably damaging	Het
Unc80	C	A	1: 66,685,731 (GRCm39)	T2285K	possibly damaging	Het
Vars1	G	A	17: 35,230,455 (GRCm39)	V513I	probably damaging	Het
Vmn2r62	T	C	7: 42,414,302 (GRCm39)	I714V	probably benign	Het
Xbp1	T	A	11: 5,474,275 (GRCm39)	S159T	probably damaging	Het
Xrn1	T	A	9: 95,851,565 (GRCm39)	H194Q	probably damaging	Het
Zc3h4	A	T	7: 16,162,976 (GRCm39)	K459*	probably null	Het
Zdhhc6	G	A	19: 55,292,871 (GRCm39)	R292*	probably null	Het
Zfp383	T	A	7: 29,608,071 (GRCm39)	M1K	probably null	Het
Zfp703	A	G	8: 27,469,044 (GRCm39)	D236G	probably damaging	Het

Other mutations in Vmn1r87

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01400:Vmn1r87	APN	7	12,866,230 (GRCm39)	missense	probably damaging	1.00
IGL01577:Vmn1r87	APN	7	12,865,775 (GRCm39)	missense	probably benign	0.00
IGL02972:Vmn1r87	APN	7	12,866,256 (GRCm39)	nonsense	probably null
IGL03246:Vmn1r87	APN	7	12,866,288 (GRCm39)	utr 5 prime	probably benign
PIT4142001:Vmn1r87	UTSW	7	12,866,112 (GRCm39)	missense	probably benign	0.00
R0153:Vmn1r87	UTSW	7	12,866,211 (GRCm39)	missense	probably damaging	1.00
R0502:Vmn1r87	UTSW	7	12,865,583 (GRCm39)	missense	probably damaging	1.00
R0658:Vmn1r87	UTSW	7	12,865,756 (GRCm39)	missense	probably damaging	1.00
R1589:Vmn1r87	UTSW	7	12,865,703 (GRCm39)	missense	possibly damaging	0.46
R1731:Vmn1r87	UTSW	7	12,865,703 (GRCm39)	missense	possibly damaging	0.46
R2027:Vmn1r87	UTSW	7	12,865,823 (GRCm39)	missense	probably damaging	0.99
R2044:Vmn1r87	UTSW	7	12,865,748 (GRCm39)	missense	probably benign	0.02
R3124:Vmn1r87	UTSW	7	12,865,493 (GRCm39)	missense	probably damaging	1.00
R4208:Vmn1r87	UTSW	7	12,866,185 (GRCm39)	missense	probably benign	0.37
R4731:Vmn1r87	UTSW	7	12,866,254 (GRCm39)	missense	possibly damaging	0.92
R4732:Vmn1r87	UTSW	7	12,866,254 (GRCm39)	missense	possibly damaging	0.92
R4733:Vmn1r87	UTSW	7	12,866,254 (GRCm39)	missense	possibly damaging	0.92
R5079:Vmn1r87	UTSW	7	12,866,253 (GRCm39)	missense	probably benign	0.01
R5125:Vmn1r87	UTSW	7	12,865,792 (GRCm39)	missense	possibly damaging	0.79
R5178:Vmn1r87	UTSW	7	12,865,792 (GRCm39)	missense	possibly damaging	0.79
R6599:Vmn1r87	UTSW	7	12,865,886 (GRCm39)	nonsense	probably null
R7560:Vmn1r87	UTSW	7	12,865,745 (GRCm39)	missense	probably damaging	1.00
R7574:Vmn1r87	UTSW	7	12,865,613 (GRCm39)	missense	probably benign	0.01
R7910:Vmn1r87	UTSW	7	12,865,832 (GRCm39)	missense	probably damaging	1.00
R8040:Vmn1r87	UTSW	7	12,866,086 (GRCm39)	missense	possibly damaging	0.87
R8220:Vmn1r87	UTSW	7	12,865,427 (GRCm39)	missense	possibly damaging	0.72
R9690:Vmn1r87	UTSW	7	12,866,263 (GRCm39)	missense	probably benign	0.01
X0028:Vmn1r87	UTSW	7	12,865,910 (GRCm39)	missense	possibly damaging	0.52

Predicted Primers

PCR Primer
(F):5'- GAAACTGTGGACATGCCGTG -3'
(R):5'- ATACAGTGTTACTAGGGGCCTTTC -3'

Sequencing Primer
(F):5'- GGACATGCCGTGTACTCCTATTG -3'
(R):5'- AGTGTATTTCAAGCCATCACTCTCAG -3'

Posted On

2019-05-13