Mutagenetix > Incidental Mutations

Incidental Mutation 'R7075:Vmn2r84'

549172

Institutional Source

Beutler Lab

Gene Symbol

Vmn2r84

Ensembl Gene

ENSMUSG00000070601

Gene Name

vomeronasal 2, receptor 84

Synonyms

EG625068

MMRRC Submission

Accession Numbers

Is this an essential gene?

Probably non essential (E-score: 0.066) question?

Stock #

R7075 (G1)

Quality Score

225.009

Status

Not validated

Chromosome

Chromosomal Location

130385316-130394241 bp(-) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

T to A at 130391072 bp

Zygosity

Heterozygous

Amino Acid Change

Glutamine to Leucine at position 299 (Q299L)

Ref Sequence

ENSEMBL: ENSMUSP00000092079 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000094502]

Predicted Effect

probably damaging
Transcript: ENSMUST00000094502
AA Change: Q299L

PolyPhen 2 Score 0.991 (Sensitivity: 0.71; Specificity: 0.97)

SMART Domains

Protein: ENSMUSP00000092079
Gene: ENSMUSG00000070601
AA Change: Q299L

Domain	Start	End	E-Value	Type
signal peptide	1	21	N/A	INTRINSIC
Pfam:ANF_receptor	77	448	1.3e-27	PFAM
Pfam:NCD3G	508	561	6.9e-21	PFAM
Pfam:7tm_3	594	830	4.6e-55	PFAM

Coding Region Coverage

1x: 100.0%
3x: 99.9%
10x: 99.7%
20x: 98.9%

Validation Efficiency

95% (60/63)

Allele List at MGI

Other mutations in this stock

Total: 62 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Acyp1	A	T	12: 85,279,008	H72Q	unknown	Het
Afmid	T	A	11: 117,835,705	D218E	probably benign	Het
Ankhd1	T	C	18: 36,559,989	V1A		Het
Atf7ip	T	C	6: 136,596,515		probably null	Het
BC024139	C	T	15: 76,124,399	V326I	probably benign	Het
Bfsp1	T	A	2: 143,848,965	Q159L	probably damaging	Het
Cct2	A	T	10: 117,061,465	W125R	unknown	Het
Cnksr3	G	T	10: 7,152,931	T147K	probably benign	Het
Colq	C	G	14: 31,552,909	G101R	probably damaging	Het
Dlg5	G	T	14: 24,177,797	T352K	possibly damaging	Het
Dusp9	TAAAGCGGAGGCCAAAGCGGAGGCCAAAGCGGAGGCTAAAGCGGAGGCCAAAGCGGAGGCCAAAGCGGAGGCCAAAGCGGAGGCTAAAGCGGAGGCCAAAGCGGAGGCCAAAG	TAAAGCGGAGGCCAAAGCGGAGGCCAAAGCGGAGGCTAAAGCGGAGGCCAAAGCGGAGGCCAAAG	X: 73,640,611		probably benign	Het
Ebna1bp2	T	C	4: 118,622,102	V119A	probably benign	Het
Eif2ak4	C	T	2: 118,420,810	Q318*	probably null	Het
Eif2b1	G	A	5: 124,571,251	T286M	probably damaging	Het
Fam53b	G	T	7: 132,759,623	D225E	probably damaging	Het
Fh1	A	G	1: 175,607,855	I354T	probably benign	Het
Fnbp1	T	C	2: 31,058,914	H206R	probably benign	Het
Gabrg3	A	T	7: 57,323,696	D74E	probably damaging	Het
Galnt18	A	T	7: 111,556,388	V246E	possibly damaging	Het
Glt6d1	T	C	2: 25,795,280	R44G	probably benign	Het
Gm17087	T	C	17: 8,566,803	M23V	probably benign	Het
Gpr160	A	T	3: 30,896,777	I333L	possibly damaging	Het
Hars	T	C	18: 36,772,355	N142S	possibly damaging	Het
Hmgcll1	T	C	9: 76,056,552	V97A	possibly damaging	Het
Itih4	T	G	14: 30,892,603	V474G	probably damaging	Het
Keap1	C	T	9: 21,231,256	V568I	probably benign	Het
Kifc5b	A	G	17: 26,925,898	M633V	probably benign	Het
Lsm4	A	G	8: 70,677,785	E18G	probably damaging	Het
Meox1	T	A	11: 101,893,743	Q88L	probably damaging	Het
Mettl25	T	C	10: 105,829,924	N147S	possibly damaging	Het
Mphosph9	A	C	5: 124,320,859	W83G	probably damaging	Het
Olfr1006	T	A	2: 85,674,824	Y109F		Het
Olfr1098	T	G	2: 86,922,646	K295N	possibly damaging	Het
Olfr344	T	C	2: 36,569,180	I194T	probably benign	Het
Olfr862	T	A	9: 19,884,063	M81L	probably benign	Het
Olfr878	T	C	9: 37,919,074	V139A	probably benign	Het
Olfr992	T	C	2: 85,400,200	D111G	probably damaging	Het
Otogl	G	T	10: 107,778,929	T1954K	probably benign	Het
Padi2	T	C	4: 140,933,217	V336A	probably damaging	Het
Phf21a	C	T	2: 92,360,379	Q675*	probably null	Het
Ppip5k1	T	C	2: 121,321,750	E1092G	probably damaging	Het
Psg21	T	C	7: 18,654,861	N102S	probably damaging	Het
Ralgapa1	C	A	12: 55,820,723	Q15H	possibly damaging	Het
Rbm26	A	T	14: 105,160,607	D26E	unknown	Het
Rbp4	T	C	19: 38,124,061	Y152C	probably damaging	Het
Recql4	A	G	15: 76,706,424	V646A	possibly damaging	Het
Rnf220	A	T	4: 117,285,882	M63K	probably benign	Het
Selenok	T	C	14: 29,970,067	S21P	probably damaging	Het
Senp1	A	G	15: 98,058,326	V404A	probably benign	Het
Sh3yl1	A	G	12: 30,940,166		probably null	Het
Snx32	A	G	19: 5,496,990	L275P	probably damaging	Het
Ssfa2	T	A	2: 79,635,660	S41T	probably damaging	Het
Stat5a	T	C	11: 100,879,693	V519A	possibly damaging	Het
Tbc1d10b	A	T	7: 127,203,238	V388E	possibly damaging	Het
Tdrd6	A	G	17: 43,625,174	V1661A	probably benign	Het
Tmem63a	T	A	1: 180,961,149	F350L	probably damaging	Het
Trio	A	T	15: 27,898,000	I401K	unknown	Het
Ttn	T	C	2: 76,716,829	E32291G	probably damaging	Het
Tubgcp5	G	T	7: 55,829,407	V1002L	probably benign	Het
Vav3	C	T	3: 109,525,924	T410I	possibly damaging	Het
Vmn1r1	A	T	1: 182,158,032	F23I	probably benign	Het
Vmn2r85	T	A	10: 130,422,688	E499D	probably benign	Het

Other mutations in Vmn2r84

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01461:Vmn2r84	APN	10	130391225	missense	possibly damaging	0.65
IGL01590:Vmn2r84	APN	10	130386095	missense	probably damaging	1.00
IGL01639:Vmn2r84	APN	10	130389272	nonsense	probably null
IGL01843:Vmn2r84	APN	10	130386279	missense	probably benign
IGL01911:Vmn2r84	APN	10	130386408	missense	probably damaging	0.99
IGL01937:Vmn2r84	APN	10	130385886	missense	probably damaging	1.00
IGL01977:Vmn2r84	APN	10	130394066	missense	probably benign	0.11
IGL02177:Vmn2r84	APN	10	130392012	missense	probably benign	0.00
IGL02291:Vmn2r84	APN	10	130390748	missense	probably damaging	1.00
IGL02590:Vmn2r84	APN	10	130391487	splice site	probably benign
IGL02727:Vmn2r84	APN	10	130394126	missense	possibly damaging	0.95
IGL02900:Vmn2r84	APN	10	130387992	splice site	probably benign
IGL03383:Vmn2r84	APN	10	130386687	missense	probably damaging	1.00
PIT4378001:Vmn2r84	UTSW	10	130385915	missense	probably damaging	1.00
R0076:Vmn2r84	UTSW	10	130394193	missense	probably damaging	1.00
R0089:Vmn2r84	UTSW	10	130386719	splice site	probably benign
R0153:Vmn2r84	UTSW	10	130392008	missense	probably benign	0.06
R0611:Vmn2r84	UTSW	10	130386122	missense	probably damaging	1.00
R0883:Vmn2r84	UTSW	10	130391115	missense	probably damaging	0.99
R1237:Vmn2r84	UTSW	10	130387856	splice site	probably null
R1295:Vmn2r84	UTSW	10	130389139	missense	probably benign	0.12
R1401:Vmn2r84	UTSW	10	130391990	missense	possibly damaging	0.89
R1521:Vmn2r84	UTSW	10	130389268	missense	probably benign	0.10
R1590:Vmn2r84	UTSW	10	130391480	critical splice acceptor site	probably null
R1710:Vmn2r84	UTSW	10	130391099	missense	probably benign	0.02
R1891:Vmn2r84	UTSW	10	130386069	missense	possibly damaging	0.78
R1956:Vmn2r84	UTSW	10	130390808	missense	probably benign	0.01
R1957:Vmn2r84	UTSW	10	130390808	missense	probably benign	0.01
R1962:Vmn2r84	UTSW	10	130390722	missense	probably damaging	0.99
R1994:Vmn2r84	UTSW	10	130386009	missense	probably damaging	1.00
R2124:Vmn2r84	UTSW	10	130391231	missense	probably damaging	0.99
R2409:Vmn2r84	UTSW	10	130392071	missense	probably damaging	0.99
R2474:Vmn2r84	UTSW	10	130386523	missense	possibly damaging	0.50
R2851:Vmn2r84	UTSW	10	130394167	missense	probably benign	0.05
R3508:Vmn2r84	UTSW	10	130390908	missense	probably damaging	1.00
R3792:Vmn2r84	UTSW	10	130385800	makesense	probably null
R4051:Vmn2r84	UTSW	10	130390898	missense	probably damaging	1.00
R4061:Vmn2r84	UTSW	10	130386029	missense	probably damaging	1.00
R4091:Vmn2r84	UTSW	10	130391369	missense	probably damaging	1.00
R4190:Vmn2r84	UTSW	10	130391294	nonsense	probably null
R4520:Vmn2r84	UTSW	10	130386522	missense	probably damaging	1.00
R4584:Vmn2r84	UTSW	10	130390713	missense	probably benign	0.00
R4588:Vmn2r84	UTSW	10	130385940	missense	probably damaging	0.98
R4655:Vmn2r84	UTSW	10	130394104	nonsense	probably null
R4860:Vmn2r84	UTSW	10	130385843	missense	probably damaging	0.99
R4860:Vmn2r84	UTSW	10	130385843	missense	probably damaging	0.99
R5022:Vmn2r84	UTSW	10	130386548	missense	probably damaging	1.00
R5146:Vmn2r84	UTSW	10	130386102	missense	probably damaging	1.00
R5237:Vmn2r84	UTSW	10	130385994	missense	probably damaging	0.99
R5695:Vmn2r84	UTSW	10	130389195	missense	probably benign	0.12
R5793:Vmn2r84	UTSW	10	130385885	missense	probably damaging	0.99
R6210:Vmn2r84	UTSW	10	130386245	missense	probably damaging	1.00
R6286:Vmn2r84	UTSW	10	130390868	missense	possibly damaging	0.65
R6580:Vmn2r84	UTSW	10	130389241	missense	possibly damaging	0.93
R6607:Vmn2r84	UTSW	10	130390862	missense	possibly damaging	0.87
R6818:Vmn2r84	UTSW	10	130386278	missense	probably benign	0.09
R6956:Vmn2r84	UTSW	10	130389267	missense	probably damaging	0.98
R6994:Vmn2r84	UTSW	10	130391007	missense	possibly damaging	0.90
R7225:Vmn2r84	UTSW	10	130386683	missense	probably damaging	0.99
R7252:Vmn2r84	UTSW	10	130386410	missense	probably damaging	1.00
R7263:Vmn2r84	UTSW	10	130389208	missense	probably damaging	1.00
R7297:Vmn2r84	UTSW	10	130391250	missense	probably benign	0.19
R7439:Vmn2r84	UTSW	10	130392113	missense	possibly damaging	0.90
R7441:Vmn2r84	UTSW	10	130392113	missense	possibly damaging	0.90
R7857:Vmn2r84	UTSW	10	130390869	missense	probably benign	0.00
R8263:Vmn2r84	UTSW	10	130391168	missense	probably damaging	1.00
R8555:Vmn2r84	UTSW	10	130394231	missense	probably benign	0.28
Z1177:Vmn2r84	UTSW	10	130391902	missense	probably damaging	1.00

Predicted Primers

PCR Primer
(F):5'- GGTTTTCGCATGTGAAATGATCC -3'
(R):5'- TGTGATCCCAGAAACCATGAAG -3'

Sequencing Primer
(F):5'- TTAATGCTGTTCTTAGAGACTGAAC -3'
(R):5'- TCCCAGAAACCATGAAGATATACATG -3'

Posted On

2019-05-15