Mutagenetix > Incidental Mutation

Incidental Mutation 'R7077:Or7e166'

549280

Institutional Source

Beutler Lab

Gene Symbol

Or7e166

Ensembl Gene

ENSMUSG00000094678

Gene Name

olfactory receptor family 7 subfamily E member 166

Synonyms

MOR146-8P, Olfr857, GA_x6K02T2PVTD-13452606-13453535

MMRRC Submission

045172-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.070) question?

Stock #

R7077 (G1)

Quality Score

225.009

Status

Validated

Chromosome

Chromosomal Location

19624125-19625054 bp(+) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

T to A at 19624428 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Serine to Threonine at position 102 (S102T)

Ref Sequence

ENSEMBL: ENSMUSP00000148617 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000077023] [ENSMUST00000212013] [ENSMUST00000217450]

AlphaFold

A0A1L1SUS1

Predicted Effect

probably benign
Transcript: ENSMUST00000077023
AA Change: S102T

PolyPhen 2 Score 0.000 (Sensitivity: 1.00; Specificity: 0.00)

SMART Domains

Protein: ENSMUSP00000076281
Gene: ENSMUSG00000094678
AA Change: S102T

Domain	Start	End	E-Value	Type
Pfam:7tm_4	31	308	2.5e-49	PFAM
Pfam:7TM_GPCR_Srsx	35	304	1.5e-5	PFAM
Pfam:7tm_1	41	290	9.4e-21	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000212013
AA Change: S102T

PolyPhen 2 Score 0.000 (Sensitivity: 1.00; Specificity: 0.00)

Predicted Effect

Coding Region Coverage

1x: 100.0%
3x: 99.9%
10x: 99.7%
20x: 98.9%

Validation Efficiency

98% (54/55)

MGI Phenotype

FUNCTION: Olfactory receptors interact with odorant molecules in the nose, to initiate a neuronal response that triggers the perception of a smell. The olfactory receptor proteins are members of a large family of G-protein-coupled receptors (GPCR) arising from single coding-exon genes. Olfactory receptors share a 7-transmembrane domain structure with many neurotransmitter and hormone receptors and are responsible for the recognition and G protein-mediated transduction of odorant signals. The olfactory receptor gene family is the largest in the genome. The nomenclature assigned to the olfactory receptor genes and proteins for this organism is independent of other organisms. [provided by RefSeq, Jul 2008]

Allele List at MGI

Other mutations in this stock

Total: 53 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Adam21	C	T	12: 81,605,893 (GRCm39)	C623Y	probably damaging	Het
Ago3	T	C	4: 126,265,325 (GRCm39)	K322R	probably null	Het
Ankrd17	T	C	5: 90,433,723 (GRCm39)	H682R	possibly damaging	Het
Aoah	A	G	13: 21,094,276 (GRCm39)	D187G	probably damaging	Het
Arhgap17	T	C	7: 122,879,231 (GRCm39)	D840G	unknown	Het
AW551984	C	T	9: 39,502,723 (GRCm39)	V650I	probably benign	Het
Bok	T	A	1: 93,616,911 (GRCm39)	Y86N	probably damaging	Het
Ccdc146	T	A	5: 21,510,272 (GRCm39)	N580I	possibly damaging	Het
Ccng2	T	C	5: 93,417,199 (GRCm39)	S72P	possibly damaging	Het
Cfap74	T	G	4: 155,540,134 (GRCm39)	I977S	unknown	Het
Cobl	T	C	11: 12,203,441 (GRCm39)	N1087S	probably benign	Het
Cyp21a1	C	A	17: 35,021,333 (GRCm39)	R346L	probably damaging	Het
Eif4a1	A	C	11: 69,561,490 (GRCm39)	F52L	probably damaging	Het
Eif4ebp2	A	C	10: 61,269,580 (GRCm39)	I120S	probably damaging	Het
Enpp2	A	C	15: 54,764,787 (GRCm39)	D146E	probably benign	Het
Exosc9	T	C	3: 36,607,205 (GRCm39)	Y30H	probably damaging	Het
Fam117a	G	A	11: 95,268,498 (GRCm39)	G300S	probably benign	Het
Focad	C	A	4: 88,328,914 (GRCm39)	A1709E	unknown	Het
Fsd1	G	A	17: 56,300,876 (GRCm39)	R245H	probably damaging	Het
Fsip2	T	C	2: 82,813,496 (GRCm39)	F3272L	probably benign	Het
Gcnt2	G	A	13: 41,013,896 (GRCm39)	M22I	probably benign	Het
Gjd4	T	C	18: 9,280,928 (GRCm39)	E50G	probably damaging	Het
Gm10375	G	T	14: 43,840,427 (GRCm39)	T162K	probably benign	Het
Gm10837	C	G	14: 122,728,142 (GRCm39)	A6G	unknown	Het
Gm4924	T	C	10: 82,215,057 (GRCm39)	F952L	unknown	Het
Heatr1	T	C	13: 12,433,045 (GRCm39)	F1132L	possibly damaging	Het
Hnrnpu	A	G	1: 178,159,756 (GRCm39)	Y442H	unknown	Het
Hp1bp3	C	A	4: 137,966,929 (GRCm39)	T408N	probably damaging	Het
Htra3	A	G	5: 35,825,660 (GRCm39)	V198A	probably damaging	Het
Katnal1	T	C	5: 148,828,547 (GRCm39)	T300A	probably benign	Het
Lipo5	G	T	19: 33,445,170 (GRCm39)	P133Q		Het
Lrp1b	A	T	2: 41,660,858 (GRCm39)	H197Q		Het
Mdc1	C	A	17: 36,156,839 (GRCm39)	A82D	probably damaging	Het
Mstn	A	T	1: 53,103,408 (GRCm39)	D248V	probably benign	Het
Myo1d	C	T	11: 80,565,460 (GRCm39)	E426K	probably damaging	Het
Ola1	G	A	2: 72,972,308 (GRCm39)	T221I	probably damaging	Het
Or52z1	A	G	7: 103,436,593 (GRCm39)	I297T	probably damaging	Het
Or5t7	T	C	2: 86,507,236 (GRCm39)	Y147C	possibly damaging	Het
Or8c20	T	A	9: 38,261,266 (GRCm39)	Y290N	probably damaging	Het
Phldb1	T	C	9: 44,623,201 (GRCm39)	T618A	possibly damaging	Het
Pkd1	T	G	17: 24,810,093 (GRCm39)	W3565G	probably damaging	Het
Prl3a1	A	T	13: 27,460,086 (GRCm39)	N190I	probably benign	Het
Ptk2	G	A	15: 73,093,658 (GRCm39)	P854S	possibly damaging	Het
Ptpn11	C	T	5: 121,281,633 (GRCm39)	R484Q	probably benign	Het
Rapgef4	T	C	2: 72,071,820 (GRCm39)	M900T	probably damaging	Het
Slc1a2	A	T	2: 102,607,855 (GRCm39)	D501V	probably benign	Het
Smarcd3	G	T	5: 24,799,960 (GRCm39)	A270D	probably damaging	Het
Srgap2	A	G	1: 131,272,187 (GRCm39)	M33T		Het
Tle1	G	C	4: 72,076,612 (GRCm39)	P139A	probably benign	Het
Tmem161b	C	A	13: 84,370,537 (GRCm39)		probably benign	Het
Tsbp1	C	A	17: 34,659,856 (GRCm39)	T93N	possibly damaging	Het
Zfp658	T	A	7: 43,223,413 (GRCm39)	S563T	probably benign	Het
Zswim9	G	A	7: 12,993,679 (GRCm39)	R826C	probably damaging	Het

Other mutations in Or7e166

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01510:Or7e166	APN	9	19,624,575 (GRCm39)	missense	probably benign	0.00
IGL01919:Or7e166	APN	9	19,624,638 (GRCm39)	missense	probably benign	0.00
IGL02157:Or7e166	APN	9	19,624,585 (GRCm39)	missense	probably benign	0.07
IGL02550:Or7e166	APN	9	19,624,343 (GRCm39)	missense	possibly damaging	0.92
IGL03329:Or7e166	APN	9	19,624,597 (GRCm39)	missense	probably benign	0.16
IGL02799:Or7e166	UTSW	9	19,624,314 (GRCm39)	missense	probably damaging	0.99
R0356:Or7e166	UTSW	9	19,624,743 (GRCm39)	missense	probably damaging	1.00
R0927:Or7e166	UTSW	9	19,624,945 (GRCm39)	missense	probably benign	0.39
R1161:Or7e166	UTSW	9	19,624,476 (GRCm39)	missense	probably damaging	1.00
R1848:Or7e166	UTSW	9	19,624,386 (GRCm39)	missense	probably benign	0.01
R5191:Or7e166	UTSW	9	19,624,630 (GRCm39)	missense	probably damaging	0.98
R5216:Or7e166	UTSW	9	19,624,585 (GRCm39)	missense	probably benign	0.07
R5259:Or7e166	UTSW	9	19,624,109 (GRCm39)	splice site	probably null
R5342:Or7e166	UTSW	9	19,624,333 (GRCm39)	missense	probably damaging	1.00
R5506:Or7e166	UTSW	9	19,624,570 (GRCm39)	missense	possibly damaging	0.61
R5526:Or7e166	UTSW	9	19,624,994 (GRCm39)	nonsense	probably null
R5594:Or7e166	UTSW	9	19,624,302 (GRCm39)	missense	probably damaging	0.99
R5928:Or7e166	UTSW	9	19,625,049 (GRCm39)	missense	probably benign	0.02
R6569:Or7e166	UTSW	9	19,624,638 (GRCm39)	missense	probably benign	0.00
R6858:Or7e166	UTSW	9	19,624,765 (GRCm39)	missense	probably damaging	0.98
R7378:Or7e166	UTSW	9	19,624,183 (GRCm39)	missense	probably damaging	1.00
R7771:Or7e166	UTSW	9	19,624,767 (GRCm39)	missense	probably benign
R8038:Or7e166	UTSW	9	19,624,976 (GRCm39)	missense	possibly damaging	0.52
R8160:Or7e166	UTSW	9	19,624,085 (GRCm39)	intron	probably benign
R8223:Or7e166	UTSW	9	19,624,705 (GRCm39)	missense	probably benign
R8400:Or7e166	UTSW	9	19,624,389 (GRCm39)	missense	probably benign	0.45
R8780:Or7e166	UTSW	9	19,624,653 (GRCm39)	missense	possibly damaging	0.92
R8946:Or7e166	UTSW	9	19,624,885 (GRCm39)	missense	probably damaging	0.99
R9164:Or7e166	UTSW	9	19,624,954 (GRCm39)	missense	probably benign	0.25
R9475:Or7e166	UTSW	9	19,624,939 (GRCm39)	missense	probably benign	0.01

Predicted Primers

PCR Primer
(F):5'- ATACATGCTCACTGTGCTTGG -3'
(R):5'- TGTCAGTTGTGATACCAATAAGCAG -3'

Sequencing Primer
(F):5'- ACATGCTCACTGTGCTTGGAAATG -3'
(R):5'- AGCAGTGTACCTGTGAACTC -3'

Posted On

2019-05-15