Incidental Mutation 'R7078:Robo2'
ID 549384
Institutional Source Beutler Lab
Gene Symbol Robo2
Ensembl Gene ENSMUSG00000052516
Gene Name roundabout guidance receptor 2
Synonyms 2600013A04Rik, 9430089E08Rik, D230004I22Rik
Accession Numbers
Essential gene? Probably essential (E-score: 0.952) question?
Stock # R7078 (G1)
Quality Score 225.009
Status Not validated
Chromosome 16
Chromosomal Location 73688727-74208713 bp(-) (GRCm39)
Type of Mutation missense
DNA Base Change (assembly) T to G at 74149504 bp (GRCm39)
Zygosity Heterozygous
Amino Acid Change Tyrosine to Serine at position 108 (Y108S)
Ref Sequence ENSEMBL: ENSMUSP00000112776 (fasta)
Gene Model predicted gene model for transcript(s): [ENSMUST00000117200] [ENSMUST00000117785] [ENSMUST00000226478] [ENSMUST00000227347]
AlphaFold no structure available at present
Predicted Effect probably damaging
Transcript: ENSMUST00000117200
AA Change: Y108S

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)
SMART Domains Protein: ENSMUSP00000113795
Gene: ENSMUSG00000052516
AA Change: Y108S

DomainStartEndE-ValueType
signal peptide 1 21 N/A INTRINSIC
IGc2 43 117 3.56e-9 SMART
IGc2 145 210 3.33e-9 SMART
IGc2 237 300 6.59e-13 SMART
IGc2 326 398 1.3e-11 SMART
IGc2 430 495 3.73e-12 SMART
FN3 522 604 1.42e-15 SMART
FN3 636 721 3.54e-2 SMART
FN3 736 823 6.15e-11 SMART
transmembrane domain 860 882 N/A INTRINSIC
low complexity region 1040 1065 N/A INTRINSIC
low complexity region 1072 1083 N/A INTRINSIC
low complexity region 1191 1199 N/A INTRINSIC
low complexity region 1210 1234 N/A INTRINSIC
low complexity region 1318 1342 N/A INTRINSIC
Predicted Effect probably damaging
Transcript: ENSMUST00000117785
AA Change: Y108S

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)
SMART Domains Protein: ENSMUSP00000112776
Gene: ENSMUSG00000052516
AA Change: Y108S

DomainStartEndE-ValueType
signal peptide 1 21 N/A INTRINSIC
IGc2 43 117 3.56e-9 SMART
IGc2 145 210 3.33e-9 SMART
IGc2 237 300 6.59e-13 SMART
IGc2 326 398 1.3e-11 SMART
IGc2 430 495 3.73e-12 SMART
FN3 522 604 1.42e-15 SMART
FN3 636 721 3.54e-2 SMART
FN3 736 823 6.15e-11 SMART
transmembrane domain 860 882 N/A INTRINSIC
low complexity region 1072 1107 N/A INTRINSIC
low complexity region 1114 1125 N/A INTRINSIC
low complexity region 1233 1241 N/A INTRINSIC
low complexity region 1252 1276 N/A INTRINSIC
low complexity region 1351 1362 N/A INTRINSIC
low complexity region 1451 1475 N/A INTRINSIC
Predicted Effect probably damaging
Transcript: ENSMUST00000226478
AA Change: Y108S

PolyPhen 2 Score 0.981 (Sensitivity: 0.75; Specificity: 0.96)
Predicted Effect probably damaging
Transcript: ENSMUST00000227347
AA Change: Y108S

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)
Coding Region Coverage
  • 1x: 100.0%
  • 3x: 99.9%
  • 10x: 99.6%
  • 20x: 98.8%
Validation Efficiency
MGI Phenotype FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] The protein encoded by this gene belongs to the ROBO family, part of the immunoglobulin superfamily of proteins that are highly conserved from fly to human. The encoded protein is a transmembrane receptor for the slit homolog 2 protein and functions in axon guidance and cell migration. Mutations in this gene are associated with vesicoureteral reflux, characterized by the backward flow of urine from the bladder into the ureters or the kidney. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2014]
PHENOTYPE: Homozygous mutants display postnatal lethality, abnormal ureteric bud development, multiple fused kidneys, multiple ureters, and abnormal commissural axon growth. [provided by MGI curators]
Allele List at MGI

All alleles(6) : Targeted(3) Gene trapped(3)

Other mutations in this stock
Total: 91 list
GeneRefVarChr/LocMutationPredicted EffectZygosity
Acaca G A 11: 84,154,138 (GRCm39) R953Q possibly damaging Het
Aldh1a1 A T 19: 20,579,434 (GRCm39) H20L probably benign Het
Ankrd52 T A 10: 128,219,526 (GRCm39) C487S probably benign Het
Ap1s3 T C 1: 79,602,845 (GRCm39) E54G probably benign Het
Arglu1 A C 8: 8,717,361 (GRCm39) M236R probably benign Het
Baz1b T A 5: 135,246,293 (GRCm39) F581I probably benign Het
Bbox1 T G 2: 110,122,884 (GRCm39) D135A probably benign Het
Bcl2l14 T A 6: 134,400,786 (GRCm39) V69E probably damaging Het
Brinp3 C T 1: 146,390,627 (GRCm39) Q58* probably null Het
Ccnb1ip1 T A 14: 51,029,724 (GRCm39) K113* probably null Het
Cep128 T C 12: 91,200,878 (GRCm39) I830M probably damaging Het
Ces2f C T 8: 105,681,284 (GRCm39) T541I probably damaging Het
Chsy3 C A 18: 59,309,149 (GRCm39) P134Q possibly damaging Het
Cobl T G 11: 12,328,271 (GRCm39) T112P probably damaging Het
Cplane2 A T 4: 140,947,159 (GRCm39) Y180F probably benign Het
Dcc T A 18: 71,680,469 (GRCm39) K589I probably benign Het
Depdc1b T A 13: 108,523,505 (GRCm39) I460K possibly damaging Het
Dlst T C 12: 85,157,705 (GRCm39) F12S probably benign Het
Dnah1 G C 14: 31,019,067 (GRCm39) F1342L probably damaging Het
Dtnb A G 12: 3,798,480 (GRCm39) Q438R possibly damaging Het
Dusp4 C A 8: 35,275,065 (GRCm39) S61R probably damaging Het
E130308A19Rik A G 4: 59,737,688 (GRCm39) Y433C probably damaging Het
Ebf2 T C 14: 67,661,407 (GRCm39) S512P probably benign Het
Elapor2 A G 5: 9,460,709 (GRCm39) N210S probably benign Het
Eya1 C T 1: 14,301,636 (GRCm39) probably null Het
Fsd1 G A 17: 56,300,876 (GRCm39) R245H probably damaging Het
Fzr1 T A 10: 81,204,463 (GRCm39) D379V probably damaging Het
Gk2 T A 5: 97,604,195 (GRCm39) E214D probably benign Het
Gm10837 C G 14: 122,728,142 (GRCm39) A6G unknown Het
Gmnc A T 16: 26,779,272 (GRCm39) V251E probably benign Het
Golga3 A G 5: 110,340,953 (GRCm39) Q549R probably damaging Het
Gsdmd T G 15: 75,736,204 (GRCm39) V111G probably damaging Het
Gtf3c1 T C 7: 125,244,914 (GRCm39) T1707A possibly damaging Het
Gucy2c C T 6: 136,674,937 (GRCm39) V1049M probably benign Het
Gucy2g A G 19: 55,229,583 (GRCm39) V29A probably damaging Het
Hecw1 A T 13: 14,609,044 (GRCm39) M1K probably null Het
Hmcn1 T C 1: 150,736,118 (GRCm39) D117G probably damaging Het
Ifnab T A 4: 88,609,350 (GRCm39) T39S possibly damaging Het
Lcorl A G 5: 45,904,566 (GRCm39) Y171H probably damaging Het
Ly6g6c C T 17: 35,288,437 (GRCm39) P103L probably damaging Het
Macf1 T G 4: 123,325,936 (GRCm39) E5189A probably damaging Het
Mapkap1 A G 2: 34,453,151 (GRCm39) E348G probably damaging Het
Med25 G T 7: 44,534,325 (GRCm39) A280D probably damaging Het
Mep1b C A 18: 21,233,108 (GRCm39) A703E probably benign Het
Morn5 T A 2: 35,944,990 (GRCm39) N71K probably benign Het
Mtmr10 T A 7: 63,970,375 (GRCm39) Y373N possibly damaging Het
Myo1d C T 11: 80,565,460 (GRCm39) E426K probably damaging Het
Ncbp1 G C 4: 46,155,756 (GRCm39) V302L probably benign Het
Notch4 A G 17: 34,801,520 (GRCm39) T1123A possibly damaging Het
Nudt18 T A 14: 70,816,452 (GRCm39) M88K possibly damaging Het
Or12k8 A G 2: 36,975,608 (GRCm39) S51P possibly damaging Het
Or5b100-ps1 A T 19: 12,994,097 (GRCm39) D170V possibly damaging Het
Or6d12 T A 6: 116,493,632 (GRCm39) L298Q probably damaging Het
Or8g4 A T 9: 39,661,787 (GRCm39) Y35F possibly damaging Het
Pdcd6ip T C 9: 113,488,953 (GRCm39) N694S probably benign Het
Pecam1 T A 11: 106,579,773 (GRCm39) T430S probably benign Het
Plaa T G 4: 94,462,288 (GRCm39) T530P probably benign Het
Ppfia3 G T 7: 45,010,019 (GRCm39) Q95K probably damaging Het
Ppp4r2 T C 6: 100,843,274 (GRCm39) S331P probably benign Het
Prg4 T C 1: 150,334,014 (GRCm39) T117A possibly damaging Het
Prrc2b A T 2: 32,103,531 (GRCm39) D1003V probably benign Het
Ptk2 G A 15: 73,093,658 (GRCm39) P854S possibly damaging Het
Rasef T C 4: 73,698,626 (GRCm39) I12V probably benign Het
Rp1 T A 1: 4,277,014 (GRCm39) E762V unknown Het
Rsrc1 T C 3: 66,901,987 (GRCm39) S46P unknown Het
Rttn A T 18: 89,027,546 (GRCm39) T554S probably benign Het
Sall3 C T 18: 81,017,314 (GRCm39) V205M probably damaging Het
Scn3a C A 2: 65,327,944 (GRCm39) V849L probably damaging Het
Sec23a A G 12: 59,039,069 (GRCm39) C277R probably benign Het
Sf3b3 C T 8: 111,539,639 (GRCm39) A1075T possibly damaging Het
Sfxn5 T C 6: 85,309,366 (GRCm39) D113G unknown Het
Slc22a22 C T 15: 57,126,876 (GRCm39) M64I probably benign Het
Slc5a11 C T 7: 122,857,669 (GRCm39) P253S probably damaging Het
Smarcd3 A G 5: 24,798,067 (GRCm39) F449S probably damaging Het
Sspo C T 6: 48,437,313 (GRCm39) T1357M probably damaging Het
Styx C T 14: 45,609,873 (GRCm39) T179I probably benign Het
Syt7 A T 19: 10,412,963 (GRCm39) T297S probably benign Het
Tbx19 T A 1: 164,988,135 (GRCm39) probably benign Het
Tg T A 15: 66,545,392 (GRCm39) V149E probably damaging Het
Tgfbr3l T A 8: 4,299,238 (GRCm39) L35Q probably damaging Het
Tmem161b C A 13: 84,370,537 (GRCm39) probably benign Het
Tmem219 T A 7: 126,490,975 (GRCm39) T161S probably damaging Het
Tox2 T A 2: 163,162,501 (GRCm39) L68H Het
Trim5 T A 7: 103,927,681 (GRCm39) D153V possibly damaging Het
Ttn T C 2: 76,580,651 (GRCm39) Y23414C probably damaging Het
Ttn A T 2: 76,582,153 (GRCm39) N22913K probably damaging Het
Ubr2 A C 17: 47,266,779 (GRCm39) M1124R possibly damaging Het
Vmn2r75 A G 7: 85,815,568 (GRCm39) S99P probably damaging Het
Wdr83 T C 8: 85,802,680 (GRCm39) D219G probably damaging Het
Wdr90 C T 17: 26,068,623 (GRCm39) V1285M probably damaging Het
Zmynd15 A T 11: 70,351,581 (GRCm39) L62F probably damaging Het
Other mutations in Robo2
AlleleSourceChrCoordTypePredicted EffectPPH Score
IGL00666:Robo2 APN 16 73,758,588 (GRCm39) missense probably benign
IGL00849:Robo2 APN 16 73,770,665 (GRCm39) missense possibly damaging 0.80
IGL00908:Robo2 APN 16 73,782,579 (GRCm39) missense probably damaging 0.98
IGL00944:Robo2 APN 16 73,730,585 (GRCm39) missense possibly damaging 0.92
IGL00955:Robo2 APN 16 73,812,860 (GRCm39) missense probably damaging 1.00
IGL00970:Robo2 APN 16 73,693,934 (GRCm39) missense probably benign 0.00
IGL01020:Robo2 APN 16 73,725,039 (GRCm39) missense probably benign 0.06
IGL01347:Robo2 APN 16 74,149,744 (GRCm39) missense probably damaging 1.00
IGL02280:Robo2 APN 16 73,843,704 (GRCm39) missense probably damaging 1.00
IGL02424:Robo2 APN 16 73,770,189 (GRCm39) missense possibly damaging 0.89
IGL03376:Robo2 APN 16 73,753,380 (GRCm39) missense probably damaging 1.00
LCD18:Robo2 UTSW 16 74,055,954 (GRCm38) intron probably benign
P0018:Robo2 UTSW 16 73,843,694 (GRCm39) missense possibly damaging 0.82
R0314:Robo2 UTSW 16 73,753,525 (GRCm39) missense probably damaging 1.00
R0324:Robo2 UTSW 16 73,764,739 (GRCm39) missense probably damaging 1.00
R0539:Robo2 UTSW 16 73,782,462 (GRCm39) splice site probably benign
R0620:Robo2 UTSW 16 73,764,690 (GRCm39) missense possibly damaging 0.92
R0630:Robo2 UTSW 16 73,713,093 (GRCm39) missense probably benign 0.05
R0701:Robo2 UTSW 16 73,843,762 (GRCm39) missense probably damaging 1.00
R1155:Robo2 UTSW 16 73,831,996 (GRCm39) missense probably damaging 1.00
R1168:Robo2 UTSW 16 73,745,184 (GRCm39) missense probably damaging 1.00
R1195:Robo2 UTSW 16 73,713,016 (GRCm39) splice site probably null
R1195:Robo2 UTSW 16 73,713,016 (GRCm39) splice site probably null
R1195:Robo2 UTSW 16 73,713,016 (GRCm39) splice site probably null
R1317:Robo2 UTSW 16 73,831,912 (GRCm39) missense probably damaging 1.00
R1422:Robo2 UTSW 16 73,775,336 (GRCm39) missense probably damaging 0.99
R1452:Robo2 UTSW 16 73,758,798 (GRCm39) missense probably damaging 1.00
R1649:Robo2 UTSW 16 73,695,889 (GRCm39) missense probably benign 0.36
R1709:Robo2 UTSW 16 73,753,411 (GRCm39) missense possibly damaging 0.83
R1751:Robo2 UTSW 16 73,831,912 (GRCm39) missense probably damaging 1.00
R1761:Robo2 UTSW 16 73,831,912 (GRCm39) missense probably damaging 1.00
R1885:Robo2 UTSW 16 73,713,033 (GRCm39) missense probably benign 0.00
R1911:Robo2 UTSW 16 73,755,213 (GRCm39) missense probably damaging 1.00
R1919:Robo2 UTSW 16 73,696,042 (GRCm39) missense probably benign
R2005:Robo2 UTSW 16 73,730,003 (GRCm39) missense possibly damaging 0.82
R2851:Robo2 UTSW 16 73,758,776 (GRCm39) missense probably damaging 1.00
R3732:Robo2 UTSW 16 73,717,635 (GRCm39) missense possibly damaging 0.64
R3732:Robo2 UTSW 16 73,717,635 (GRCm39) missense possibly damaging 0.64
R3733:Robo2 UTSW 16 73,717,635 (GRCm39) missense possibly damaging 0.64
R3734:Robo2 UTSW 16 73,717,635 (GRCm39) missense possibly damaging 0.64
R3913:Robo2 UTSW 16 73,831,893 (GRCm39) missense probably damaging 1.00
R3956:Robo2 UTSW 16 73,758,755 (GRCm39) missense probably damaging 1.00
R4394:Robo2 UTSW 16 73,745,267 (GRCm39) missense probably benign 0.13
R4426:Robo2 UTSW 16 73,745,154 (GRCm39) missense probably damaging 1.00
R4437:Robo2 UTSW 16 73,770,132 (GRCm39) missense possibly damaging 0.88
R4454:Robo2 UTSW 16 74,149,407 (GRCm39) intron probably benign
R4478:Robo2 UTSW 16 73,812,761 (GRCm39) missense probably damaging 1.00
R4586:Robo2 UTSW 16 73,758,761 (GRCm39) missense probably damaging 0.96
R4621:Robo2 UTSW 16 73,782,821 (GRCm39) missense probably benign 0.00
R4673:Robo2 UTSW 16 73,701,266 (GRCm39) splice site probably null
R4798:Robo2 UTSW 16 74,149,633 (GRCm39) missense probably damaging 1.00
R4812:Robo2 UTSW 16 73,713,176 (GRCm39) missense probably benign 0.00
R4855:Robo2 UTSW 16 73,768,079 (GRCm39) missense probably damaging 1.00
R4910:Robo2 UTSW 16 73,730,666 (GRCm39) missense probably damaging 0.99
R4916:Robo2 UTSW 16 73,695,803 (GRCm39) missense possibly damaging 0.53
R4948:Robo2 UTSW 16 74,149,726 (GRCm39) missense possibly damaging 0.88
R5325:Robo2 UTSW 16 73,770,673 (GRCm39) missense possibly damaging 0.72
R5326:Robo2 UTSW 16 73,695,853 (GRCm39) missense probably benign 0.20
R5447:Robo2 UTSW 16 73,770,654 (GRCm39) nonsense probably null
R5542:Robo2 UTSW 16 73,695,853 (GRCm39) missense probably benign 0.20
R5545:Robo2 UTSW 16 73,758,635 (GRCm39) missense probably damaging 1.00
R5646:Robo2 UTSW 16 73,758,707 (GRCm39) missense probably damaging 0.99
R5734:Robo2 UTSW 16 74,149,672 (GRCm39) missense probably damaging 1.00
R5892:Robo2 UTSW 16 73,692,668 (GRCm39) utr 3 prime probably benign
R5960:Robo2 UTSW 16 73,730,603 (GRCm39) missense probably damaging 1.00
R6126:Robo2 UTSW 16 73,717,570 (GRCm39) missense probably benign 0.00
R6130:Robo2 UTSW 16 73,717,570 (GRCm39) missense probably benign 0.00
R6153:Robo2 UTSW 16 73,717,617 (GRCm39) missense probably damaging 1.00
R6240:Robo2 UTSW 16 73,779,027 (GRCm39) missense probably damaging 1.00
R6247:Robo2 UTSW 16 73,764,672 (GRCm39) missense probably damaging 1.00
R6304:Robo2 UTSW 16 73,755,196 (GRCm39) missense probably damaging 1.00
R6337:Robo2 UTSW 16 73,725,039 (GRCm39) missense probably benign 0.06
R6431:Robo2 UTSW 16 73,843,697 (GRCm39) nonsense probably null
R6440:Robo2 UTSW 16 73,713,010 (GRCm39) missense probably benign 0.31
R6596:Robo2 UTSW 16 73,767,996 (GRCm39) missense probably damaging 1.00
R6919:Robo2 UTSW 16 73,758,755 (GRCm39) missense probably damaging 1.00
R6927:Robo2 UTSW 16 73,778,946 (GRCm39) missense probably damaging 1.00
R7029:Robo2 UTSW 16 73,745,225 (GRCm39) missense probably damaging 1.00
R7092:Robo2 UTSW 16 73,753,531 (GRCm39) missense probably damaging 0.99
R7136:Robo2 UTSW 16 73,753,438 (GRCm39) missense probably damaging 0.99
R7192:Robo2 UTSW 16 73,717,638 (GRCm39) missense probably benign 0.19
R7569:Robo2 UTSW 16 73,832,003 (GRCm39) missense possibly damaging 0.82
R7686:Robo2 UTSW 16 73,755,293 (GRCm39) missense probably damaging 1.00
R7720:Robo2 UTSW 16 73,693,903 (GRCm39) missense probably benign 0.00
R7772:Robo2 UTSW 16 73,758,777 (GRCm39) missense probably benign 0.24
R7822:Robo2 UTSW 16 73,770,059 (GRCm39) missense probably damaging 1.00
R7849:Robo2 UTSW 16 73,770,132 (GRCm39) missense possibly damaging 0.88
R7881:Robo2 UTSW 16 73,717,585 (GRCm39) missense probably benign 0.00
R7897:Robo2 UTSW 16 73,695,838 (GRCm39) missense probably benign
R8135:Robo2 UTSW 16 73,730,048 (GRCm39) missense probably benign 0.04
R8297:Robo2 UTSW 16 73,812,814 (GRCm39) nonsense probably null
R8307:Robo2 UTSW 16 73,753,498 (GRCm39) missense probably damaging 1.00
R8379:Robo2 UTSW 16 73,730,588 (GRCm39) missense probably damaging 0.99
R8393:Robo2 UTSW 16 73,775,382 (GRCm39) missense probably damaging 1.00
R8474:Robo2 UTSW 16 73,745,150 (GRCm39) missense probably damaging 1.00
R8500:Robo2 UTSW 16 73,745,228 (GRCm39) missense probably damaging 1.00
R8721:Robo2 UTSW 16 73,703,798 (GRCm39) missense
R8734:Robo2 UTSW 16 73,764,651 (GRCm39) splice site probably benign
R8735:Robo2 UTSW 16 73,755,247 (GRCm39) missense probably damaging 1.00
R8840:Robo2 UTSW 16 73,782,570 (GRCm39) missense probably damaging 1.00
R8937:Robo2 UTSW 16 73,770,150 (GRCm39) missense probably damaging 1.00
R8937:Robo2 UTSW 16 73,770,149 (GRCm39) missense probably damaging 1.00
R8968:Robo2 UTSW 16 73,767,941 (GRCm39) critical splice donor site probably null
R9134:Robo2 UTSW 16 73,703,738 (GRCm39) missense
R9622:Robo2 UTSW 16 73,729,952 (GRCm39) missense probably benign 0.02
R9662:Robo2 UTSW 16 73,758,566 (GRCm39) critical splice donor site probably null
R9708:Robo2 UTSW 16 73,770,197 (GRCm39) missense possibly damaging 0.70
R9779:Robo2 UTSW 16 73,767,965 (GRCm39) missense probably damaging 0.97
X0063:Robo2 UTSW 16 73,842,716 (GRCm39) missense probably damaging 1.00
Z1176:Robo2 UTSW 16 73,730,479 (GRCm39) missense probably benign 0.35
Z1177:Robo2 UTSW 16 73,737,187 (GRCm39) critical splice acceptor site probably null
Predicted Primers PCR Primer
(F):5'- ATTCCGAGATGCAGAGATAGTG -3'
(R):5'- CTGATGTCATCGTCTCCAAGG -3'

Sequencing Primer
(F):5'- CGAGATGCAGAGATAGTGTATTATTC -3'
(R):5'- GAGAGCCCACCACTCTGAACTG -3'
Posted On 2019-05-15