Mutagenetix > Incidental Mutation

Incidental Mutation 'R7090:Vmn2r99'

550179

Institutional Source

Beutler Lab

Gene Symbol

Vmn2r99

Ensembl Gene

ENSMUSG00000090304

Gene Name

vomeronasal 2, receptor 99

Synonyms

EG665376

MMRRC Submission

045184-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.131) question?

Stock #

R7090 (G1)

Quality Score

225.009

Status

Validated

Chromosome

Chromosomal Location

19361949-19401098 bp(+) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

A to T at 19393710 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Glutamic Acid to Valine at position 564 (E564V)

Ref Sequence

ENSEMBL: ENSMUSP00000135236 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000176107] [ENSMUST00000231989]

AlphaFold

H3BK37

Predicted Effect

possibly damaging
Transcript: ENSMUST00000176107
AA Change: E564V

PolyPhen 2 Score 0.828 (Sensitivity: 0.84; Specificity: 0.93)

SMART Domains

Protein: ENSMUSP00000135236
Gene: ENSMUSG00000090304
AA Change: E564V

Domain	Start	End	E-Value	Type
signal peptide	1	19	N/A	INTRINSIC
Pfam:ANF_receptor	81	448	5.7e-33	PFAM
Pfam:NCD3G	508	561	1.8e-21	PFAM
Pfam:7tm_3	593	829	4.6e-52	PFAM

Predicted Effect

unknown
Transcript: ENSMUST00000231989
AA Change: R606S

Coding Region Coverage

1x: 100.0%
3x: 100.0%
10x: 99.7%
20x: 99.1%

Validation Efficiency

100% (66/66)

Allele List at MGI

Other mutations in this stock

Total: 65 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Calm3	A	T	7: 16,917,079 (GRCm38)	Y139*	probably null	Het
Ccdc27	T	C	4: 154,028,066 (GRCm38)	N584S	probably benign	Het
Cdh24	C	G	14: 54,639,507 (GRCm38)	G13A	probably damaging	Het
Chd8	T	C	14: 52,215,220 (GRCm38)	K1281E	probably damaging	Het
Clasp1	G	T	1: 118,482,086 (GRCm38)	G4C	probably benign	Het
Cul9	G	T	17: 46,500,839 (GRCm38)	P2488T	probably damaging	Het
Daam2	A	G	17: 49,482,945 (GRCm38)	V428A	probably damaging	Het
Dcaf8	A	G	1: 172,188,968 (GRCm38)	S441G	probably damaging	Het
Dchs2	A	G	3: 83,348,274 (GRCm38)	T2426A	probably benign	Het
Dpys	A	G	15: 39,826,883 (GRCm38)	V358A	probably damaging	Het
Efhd1	A	G	1: 87,289,497 (GRCm38)	D112G	probably damaging	Het
Exoc1	T	C	5: 76,566,953 (GRCm38)	S120P	unknown	Het
Fyco1	A	G	9: 123,797,719 (GRCm38)	L1309S	probably damaging	Het
Gm14403	A	G	2: 177,509,321 (GRCm38)	N111S	possibly damaging	Het
Gm5901	A	G	7: 105,377,348 (GRCm38)	T108A	probably benign	Het
Idh2	TCCCAGG	T	7: 80,098,331 (GRCm38)		probably benign	Het
Itgb5	T	G	16: 33,885,094 (GRCm38)	D251E	probably damaging	Het
Kcnk15	T	C	2: 163,858,717 (GRCm38)	V292A	probably benign	Het
Kdm2a	A	T	19: 4,319,141 (GRCm38)	Y1149N	probably damaging	Het
Kdm3a	T	C	6: 71,595,545 (GRCm38)	T1011A	possibly damaging	Het
Krt35	A	T	11: 100,095,672 (GRCm38)		probably null	Het
Lipn	T	A	19: 34,071,780 (GRCm38)	D115E	possibly damaging	Het
Lpin3	T	G	2: 160,896,752 (GRCm38)	L208R	probably damaging	Het
Ltf	C	A	9: 111,025,980 (GRCm38)	Q354K	probably benign	Het
Marf1	C	A	16: 14,111,702 (GRCm38)	C1680F	possibly damaging	Het
Mtss1l	T	A	8: 110,730,024 (GRCm38)	V242E	probably damaging	Het
Myo7b	T	C	18: 31,998,712 (GRCm38)	Y477C	probably damaging	Het
Nbn	T	A	4: 15,981,350 (GRCm38)	S481T	probably benign	Het
Ncoa2	A	G	1: 13,186,838 (GRCm38)	Y146H	probably damaging	Het
Ntng1	A	T	3: 109,935,180 (GRCm38)	C92*	probably null	Het
Olfr1049	T	A	2: 86,255,076 (GRCm38)	N206Y	probably damaging	Het
Olfr1077-ps1	T	C	2: 86,526,127 (GRCm38)	I17V	probably benign	Het
Olfr133	G	A	17: 38,149,494 (GRCm38)	G302D	probably benign	Het
Olfr286	C	T	15: 98,227,202 (GRCm38)	V150I	probably benign	Het
Os9	A	T	10: 127,099,678 (GRCm38)	S308T	probably benign	Het
Otx2	T	A	14: 48,658,735 (GRCm38)	T289S	probably benign	Het
Palm2	C	T	4: 57,648,042 (GRCm38)	A60V	probably benign	Het
Pigg	A	G	5: 108,336,512 (GRCm38)	T675A	possibly damaging	Het
Pink1	C	T	4: 138,315,601 (GRCm38)	E461K	probably damaging	Het
Pip5k1a	A	G	3: 95,060,498 (GRCm38)	S543P	possibly damaging	Het
Prkaa1	A	G	15: 5,177,130 (GRCm38)	T454A	probably benign	Het
Ptk2	G	A	15: 73,221,809 (GRCm38)	P854S	possibly damaging	Het
Rab31	C	T	17: 65,698,017 (GRCm38)	V83I	possibly damaging	Het
Rabggtb	G	T	3: 153,910,349 (GRCm38)	D117E	probably benign	Het
Rbl1	T	C	2: 157,152,900 (GRCm38)	H951R	probably benign	Het
Sapcd2	A	G	2: 25,376,079 (GRCm38)	S314G	probably benign	Het
Sept4	T	C	11: 87,584,438 (GRCm38)	L164P	probably damaging	Het
Shroom1	A	G	11: 53,465,933 (GRCm38)	E541G	probably damaging	Het
Slc26a7	C	A	4: 14,565,460 (GRCm38)	G208*	probably null	Het
Smco2	A	G	6: 146,871,213 (GRCm38)	I304M	probably damaging	Het
Spg7	T	C	8: 123,091,752 (GRCm38)		probably null	Het
Spocd1	T	C	4: 129,953,898 (GRCm38)	F552L		Het
St6galnac2	T	C	11: 116,677,635 (GRCm38)	D334G	probably damaging	Het
Sub1	A	G	15: 11,986,486 (GRCm38)	S92P	probably benign	Het
Syne2	T	C	12: 75,942,351 (GRCm38)	F1829L	probably benign	Het
Tdrd1	T	C	19: 56,851,401 (GRCm38)	V631A	probably benign	Het
Tdrd9	T	C	12: 111,992,470 (GRCm38)	S113P	probably benign	Het
Tgfbrap1	A	C	1: 43,071,565 (GRCm38)	V260G	probably damaging	Het
Tyw3	G	C	3: 154,593,789 (GRCm38)	S94R	probably benign	Het
Urb2	T	C	8: 124,030,599 (GRCm38)	V1015A	probably benign	Het
Usp5	A	G	6: 124,829,394 (GRCm38)	M1T	probably null	Het
Utrn	T	C	10: 12,684,516 (GRCm38)	D1343G	possibly damaging	Het
Vmn2r56	T	C	7: 12,715,327 (GRCm38)	Y328C	probably damaging	Het
Wdr63	C	A	3: 146,040,827 (GRCm38)	K881N	possibly damaging	Het
Zc3h8	T	C	2: 128,935,321 (GRCm38)	T133A	possibly damaging	Het

Other mutations in Vmn2r99

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00503:Vmn2r99	APN	17	19,378,854 (GRCm38)	missense	probably benign	0.01
IGL01113:Vmn2r99	APN	17	19,394,256 (GRCm38)	missense	probably benign	0.20
IGL01138:Vmn2r99	APN	17	19,382,623 (GRCm38)	missense	probably damaging	0.97
IGL01646:Vmn2r99	APN	17	19,393,658 (GRCm38)	splice site	probably benign
IGL01769:Vmn2r99	APN	17	19,380,115 (GRCm38)	missense	probably damaging	1.00
IGL02112:Vmn2r99	APN	17	19,380,232 (GRCm38)	missense	probably null	0.99
IGL02891:Vmn2r99	APN	17	19,378,690 (GRCm38)	nonsense	probably null
IGL03132:Vmn2r99	APN	17	19,378,223 (GRCm38)	nonsense	probably null
FR4548:Vmn2r99	UTSW	17	19,394,285 (GRCm38)	missense	probably damaging	0.97
FR4976:Vmn2r99	UTSW	17	19,394,285 (GRCm38)	missense	probably damaging	0.97
PIT4382001:Vmn2r99	UTSW	17	19,394,343 (GRCm38)	missense	probably damaging	1.00
R0196:Vmn2r99	UTSW	17	19,394,573 (GRCm38)	missense	probably benign	0.00
R0720:Vmn2r99	UTSW	17	19,379,043 (GRCm38)	missense	probably benign	0.00
R1501:Vmn2r99	UTSW	17	19,362,259 (GRCm38)	missense	possibly damaging	0.93
R1519:Vmn2r99	UTSW	17	19,380,060 (GRCm38)	missense	probably benign	0.00
R1670:Vmn2r99	UTSW	17	19,362,252 (GRCm38)	missense	probably benign	0.37
R1682:Vmn2r99	UTSW	17	19,377,945 (GRCm38)	missense	probably damaging	0.97
R1873:Vmn2r99	UTSW	17	19,362,153 (GRCm38)	missense	probably benign	0.25
R1967:Vmn2r99	UTSW	17	19,378,815 (GRCm38)	missense	probably benign	0.01
R2101:Vmn2r99	UTSW	17	19,377,991 (GRCm38)	missense	probably damaging	1.00
R2474:Vmn2r99	UTSW	17	19,378,629 (GRCm38)	missense	probably benign	0.04
R2519:Vmn2r99	UTSW	17	19,378,708 (GRCm38)	missense	probably damaging	0.99
R3911:Vmn2r99	UTSW	17	19,394,373 (GRCm38)	missense	possibly damaging	0.92
R3947:Vmn2r99	UTSW	17	19,378,990 (GRCm38)	missense	probably benign	0.40
R3949:Vmn2r99	UTSW	17	19,378,990 (GRCm38)	missense	probably benign	0.40
R4016:Vmn2r99	UTSW	17	19,378,570 (GRCm38)	missense	possibly damaging	0.86
R4413:Vmn2r99	UTSW	17	19,379,260 (GRCm38)	missense	probably damaging	1.00
R4594:Vmn2r99	UTSW	17	19,393,662 (GRCm38)	missense	probably damaging	1.00
R4999:Vmn2r99	UTSW	17	19,362,135 (GRCm38)	start codon destroyed	probably null	0.96
R5206:Vmn2r99	UTSW	17	19,378,606 (GRCm38)	missense	probably benign	0.40
R5362:Vmn2r99	UTSW	17	19,379,339 (GRCm38)	missense	probably benign	0.00
R5377:Vmn2r99	UTSW	17	19,379,269 (GRCm38)	missense	probably damaging	1.00
R5455:Vmn2r99	UTSW	17	19,394,146 (GRCm38)	nonsense	probably null
R6021:Vmn2r99	UTSW	17	19,377,948 (GRCm38)	missense	probably damaging	1.00
R6059:Vmn2r99	UTSW	17	19,378,980 (GRCm38)	missense	probably benign	0.00
R6214:Vmn2r99	UTSW	17	19,382,558 (GRCm38)	missense	probably benign	0.19
R6215:Vmn2r99	UTSW	17	19,382,558 (GRCm38)	missense	probably benign	0.19
R6313:Vmn2r99	UTSW	17	19,382,605 (GRCm38)	missense	probably damaging	1.00
R6646:Vmn2r99	UTSW	17	19,380,031 (GRCm38)	missense	probably damaging	1.00
R6810:Vmn2r99	UTSW	17	19,380,034 (GRCm38)	missense	probably benign	0.20
R6885:Vmn2r99	UTSW	17	19,380,195 (GRCm38)	missense	possibly damaging	0.52
R6991:Vmn2r99	UTSW	17	19,378,110 (GRCm38)	missense	probably benign	0.03
R7060:Vmn2r99	UTSW	17	19,394,564 (GRCm38)	nonsense	probably null
R7094:Vmn2r99	UTSW	17	19,379,311 (GRCm38)	missense	probably benign	0.00
R7449:Vmn2r99	UTSW	17	19,379,145 (GRCm38)	missense	probably benign	0.01
R7789:Vmn2r99	UTSW	17	19,393,817 (GRCm38)	missense	possibly damaging	0.91
R8039:Vmn2r99	UTSW	17	19,380,040 (GRCm38)	missense	probably benign	0.00
R8493:Vmn2r99	UTSW	17	19,393,758 (GRCm38)	missense	probably benign	0.15
R8511:Vmn2r99	UTSW	17	19,394,181 (GRCm38)	missense	probably damaging	1.00
R8715:Vmn2r99	UTSW	17	19,393,660 (GRCm38)	critical splice acceptor site	probably benign
R9462:Vmn2r99	UTSW	17	19,378,126 (GRCm38)	nonsense	probably null
R9681:Vmn2r99	UTSW	17	19,378,627 (GRCm38)	missense	probably damaging	1.00
R9737:Vmn2r99	UTSW	17	19,362,301 (GRCm38)	missense	probably benign
Z1088:Vmn2r99	UTSW	17	19,379,301 (GRCm38)	missense	probably benign	0.18

Predicted Primers

PCR Primer
(F):5'- AAGAACAAGGAGCAGTCTCTAACTC -3'
(R):5'- TAACTCAGAGCTCGATTATTTGCC -3'

Sequencing Primer
(F):5'- CCCTGCAGTTAGAGATGT -3'
(R):5'- CCTTAACAATAGGAGTGTCTCTGTGC -3'

Posted On

2019-05-15