Mutagenetix > Incidental Mutation

Incidental Mutation 'R7091:Vmn2r27'

550212

Institutional Source

Beutler Lab

Gene Symbol

Vmn2r27

Ensembl Gene

ENSMUSG00000072778

Gene Name

vomeronasal 2, receptor27

Synonyms

EG232367

MMRRC Submission

045185-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.077) question?

Stock #

R7091 (G1)

Quality Score

225.009

Status

Validated

Chromosome

Chromosomal Location

124191596-124231784 bp(-) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

T to G at 124223945 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Glutamine to Proline at position 351 (Q351P)

Ref Sequence

ENSEMBL: ENSMUSP00000098528 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000100968]

AlphaFold

D3YUK6

Predicted Effect

possibly damaging
Transcript: ENSMUST00000100968
AA Change: Q351P

PolyPhen 2 Score 0.851 (Sensitivity: 0.83; Specificity: 0.93)

SMART Domains

Protein: ENSMUSP00000098528
Gene: ENSMUSG00000072778
AA Change: Q351P

Domain	Start	End	E-Value	Type
signal peptide	1	22	N/A	INTRINSIC
Pfam:ANF_receptor	81	475	1.1e-27	PFAM
Pfam:NCD3G	519	570	1.3e-18	PFAM
Pfam:7tm_3	603	838	2.6e-50	PFAM

Coding Region Coverage

1x: 100.0%
3x: 100.0%
10x: 99.7%
20x: 99.1%

Validation Efficiency

98% (56/57)

Allele List at MGI

Other mutations in this stock

Total: 58 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
9130019O22Rik	C	A	7: 127,384,362	A523S	possibly damaging	Het
Abhd18	A	C	3: 40,916,738	I111L	probably damaging	Het
Ank1	A	G	8: 23,058,663	D11G	probably benign	Het
Ank2	G	T	3: 127,023,351	Q472K	probably damaging	Het
Apbb2	A	T	5: 66,313,334	L520H	probably damaging	Het
Baat	T	C	4: 49,499,692	K205E	probably benign	Het
Brca1	T	A	11: 101,526,427	M294L	probably benign	Het
Capn1	A	G	19: 5,991,556	M641T	possibly damaging	Het
Cluap1	T	A	16: 3,940,806	D377E	probably benign	Het
Col6a2	C	T	10: 76,615,091	V39I	unknown	Het
Crybg3	T	A	16: 59,557,168	D1241V	possibly damaging	Het
Dnah10	A	G	5: 124,816,142	K3380R	probably benign	Het
Eml5	T	C	12: 98,802,474	I1400M	probably benign	Het
Fancd2	A	G	6: 113,545,101	D219G	probably damaging	Het
Fras1	A	G	5: 96,708,676	S1973G	probably benign	Het
Fsd1	G	A	17: 55,993,876	R245H	probably damaging	Het
G3bp1	T	A	11: 55,496,221	H271Q	possibly damaging	Het
Glce	A	G	9: 62,060,588	V427A	probably damaging	Het
Gm4778	T	C	3: 94,266,638	F314L	probably damaging	Het
Gm5141	T	C	13: 62,773,964	T464A	possibly damaging	Het
Gulp1	T	A	1: 44,766,134	F128I	probably damaging	Het
H2-Bl	T	C	17: 36,083,941	E30G	possibly damaging	Het
Hcrtr1	A	C	4: 130,130,914	L393W	probably damaging	Het
Heg1	T	C	16: 33,726,720	S650P	probably benign	Het
Hspa4l	T	A	3: 40,781,592	N569K	probably benign	Het
Ifi206	A	G	1: 173,473,875	F746L	unknown	Het
Ivl	T	C	3: 92,572,242	D172G	possibly damaging	Het
Lrp5	A	T	19: 3,630,184	D433E	probably damaging	Het
Mgam	T	C	6: 40,768,276	S1826P	possibly damaging	Het
Ms4a18	A	T	19: 11,008,728	L206M	probably damaging	Het
Msln	A	T	17: 25,750,080	C444S	probably damaging	Het
Mta1	A	G	12: 113,136,402	D644G	probably damaging	Het
Muc5ac	G	C	7: 141,809,687		probably benign	Het
Naa15	T	C	3: 51,458,756		probably null	Het
Nadk	A	G	4: 155,587,758	H302R	probably benign	Het
Neb	T	A	2: 52,256,112	N15I		Het
Nup153	A	T	13: 46,683,928	S1273T	probably benign	Het
Ofcc1	A	G	13: 40,072,767	I763T	probably damaging	Het
Olfr142	T	C	2: 90,252,463	Y175C	probably damaging	Het
Oxsr1	T	C	9: 119,284,661	I107V	probably benign	Het
Prmt5	A	G	14: 54,511,342		probably null	Het
Ptk2	G	A	15: 73,221,809	P854S	possibly damaging	Het
Ranbp6	A	G	19: 29,812,716	S79P	probably damaging	Het
Reln	T	C	5: 21,899,029	I3315V	probably null	Het
Rnf223	T	C	4: 156,132,699	V177A	probably benign	Het
Slc20a1	C	T	2: 129,208,272	T450M	possibly damaging	Het
Smg5	C	T	3: 88,351,347	P542S	probably benign	Het
Sorl1	T	A	9: 42,002,634	Q1333L	probably benign	Het
Spag5	T	A	11: 78,313,191		probably null	Het
Tdp2	T	A	13: 24,838,224	F209I	probably damaging	Het
Tgm4	C	A	9: 123,040,460	L35M	probably damaging	Het
Tma7	A	G	9: 109,082,512		probably benign	Het
Tmprss4	A	T	9: 45,184,273	V91D	probably damaging	Het
Tnfsf4	T	A	1: 161,395,697	M39K	probably benign	Het
Ttn	T	A	2: 76,713,568	T33025S	probably benign	Het
Tut1	A	G	19: 8,965,811	H754R	probably benign	Het
Wee2	G	T	6: 40,462,002	G353V	probably benign	Het
Zfp879	T	A	11: 50,833,395	H278L	probably damaging	Het

Other mutations in Vmn2r27

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01285:Vmn2r27	APN	6	124192411	missense	possibly damaging	0.86
IGL01388:Vmn2r27	APN	6	124223832	missense	possibly damaging	0.55
IGL01923:Vmn2r27	APN	6	124200525	missense	probably benign	0.20
IGL01954:Vmn2r27	APN	6	124192248	missense	probably damaging	1.00
IGL02105:Vmn2r27	APN	6	124197349	splice site	probably benign
IGL02586:Vmn2r27	APN	6	124224475	nonsense	probably null
IGL03130:Vmn2r27	APN	6	124192317	missense	possibly damaging	0.82
IGL03330:Vmn2r27	APN	6	124230180	nonsense	probably null
R0124:Vmn2r27	UTSW	6	124231619	missense	probably benign
R0234:Vmn2r27	UTSW	6	124231619	missense	probably benign
R0234:Vmn2r27	UTSW	6	124231619	missense	probably benign
R0384:Vmn2r27	UTSW	6	124223912	missense	probably benign	0.01
R0582:Vmn2r27	UTSW	6	124224290	missense	probably benign	0.02
R0733:Vmn2r27	UTSW	6	124192188	missense	probably benign	0.18
R0738:Vmn2r27	UTSW	6	124223702	missense	possibly damaging	0.48
R0835:Vmn2r27	UTSW	6	124200624	missense	probably damaging	0.99
R1183:Vmn2r27	UTSW	6	124200532	missense	probably benign
R1401:Vmn2r27	UTSW	6	124191632	nonsense	probably null
R1484:Vmn2r27	UTSW	6	124200515	missense	probably damaging	0.96
R1536:Vmn2r27	UTSW	6	124200690	missense	probably damaging	1.00
R1539:Vmn2r27	UTSW	6	124191771	missense	probably damaging	1.00
R1565:Vmn2r27	UTSW	6	124231634	missense	probably benign
R1595:Vmn2r27	UTSW	6	124231615	missense	probably benign	0.00
R1614:Vmn2r27	UTSW	6	124223934	missense	probably benign	0.01
R1742:Vmn2r27	UTSW	6	124200677	missense	possibly damaging	0.48
R1816:Vmn2r27	UTSW	6	124230371	nonsense	probably null
R1822:Vmn2r27	UTSW	6	124231634	missense	probably benign
R1824:Vmn2r27	UTSW	6	124231634	missense	probably benign
R1870:Vmn2r27	UTSW	6	124224211	missense	probably benign	0.11
R1942:Vmn2r27	UTSW	6	124223763	missense	probably damaging	1.00
R1962:Vmn2r27	UTSW	6	124223834	missense	possibly damaging	0.70
R2069:Vmn2r27	UTSW	6	124224483	missense	probably damaging	1.00
R2075:Vmn2r27	UTSW	6	124200551	missense	possibly damaging	0.85
R2379:Vmn2r27	UTSW	6	124224383	missense	possibly damaging	0.89
R3748:Vmn2r27	UTSW	6	124230392	missense	probably benign	0.35
R4384:Vmn2r27	UTSW	6	124224156	missense	probably benign	0.05
R4392:Vmn2r27	UTSW	6	124230176	missense	probably benign	0.01
R4758:Vmn2r27	UTSW	6	124231637	missense	possibly damaging	0.87
R5018:Vmn2r27	UTSW	6	124224182	missense	probably benign	0.02
R5235:Vmn2r27	UTSW	6	124192054	missense	probably damaging	0.99
R5718:Vmn2r27	UTSW	6	124192144	missense	possibly damaging	0.66
R5859:Vmn2r27	UTSW	6	124200688	missense	probably damaging	1.00
R5958:Vmn2r27	UTSW	6	124231727	missense	probably benign	0.00
R6044:Vmn2r27	UTSW	6	124231772	missense	probably benign
R6086:Vmn2r27	UTSW	6	124191999	missense	probably damaging	1.00
R6396:Vmn2r27	UTSW	6	124224166	nonsense	probably null
R6546:Vmn2r27	UTSW	6	124192410	missense	possibly damaging	0.49
R6746:Vmn2r27	UTSW	6	124200593	missense	possibly damaging	0.47
R6976:Vmn2r27	UTSW	6	124224353	nonsense	probably null
R7145:Vmn2r27	UTSW	6	124191752	missense	probably benign
R7176:Vmn2r27	UTSW	6	124192036	missense	probably benign	0.01
R7382:Vmn2r27	UTSW	6	124197317	missense	probably damaging	1.00
R7482:Vmn2r27	UTSW	6	124224261	missense	probably damaging	1.00
R7853:Vmn2r27	UTSW	6	124192021	missense	probably damaging	1.00
R7859:Vmn2r27	UTSW	6	124224242	missense	probably benign	0.00
R7959:Vmn2r27	UTSW	6	124192081	missense	probably benign
R8266:Vmn2r27	UTSW	6	124191978	missense	probably benign	0.00
R8353:Vmn2r27	UTSW	6	124192445	missense	probably damaging	0.99
R8394:Vmn2r27	UTSW	6	124191817	missense	possibly damaging	0.71
R8463:Vmn2r27	UTSW	6	124192209	missense	probably damaging	1.00
R8477:Vmn2r27	UTSW	6	124224241	missense	probably benign	0.11
R8705:Vmn2r27	UTSW	6	124230229	missense	probably damaging	1.00
R8752:Vmn2r27	UTSW	6	124224059	missense	probably benign	0.00
R9109:Vmn2r27	UTSW	6	124197265	missense	possibly damaging	0.95
R9140:Vmn2r27	UTSW	6	124192248	missense	probably damaging	1.00
R9157:Vmn2r27	UTSW	6	124224285	missense	probably benign	0.09
R9431:Vmn2r27	UTSW	6	124191897	missense	probably damaging	1.00
R9477:Vmn2r27	UTSW	6	124191951	missense	probably damaging	0.99
R9758:Vmn2r27	UTSW	6	124191678	missense	possibly damaging	0.89
Z1177:Vmn2r27	UTSW	6	124191901	missense	probably damaging	1.00

Predicted Primers

PCR Primer
(F):5'- TCCACTCTGACTGACAGCTC -3'
(R):5'- CGTTCTTGCCTTTTAATGGCAATG -3'

Sequencing Primer
(F):5'- TCTGACTGACAGCTCCACATGG -3'
(R):5'- GCCTTTTAATGGCAATGTCTTGATC -3'

Posted On

2019-05-15