Mutagenetix > Incidental Mutation

Incidental Mutation 'R7094:Map2'

550333

Institutional Source

Beutler Lab

Gene Symbol

Map2

Ensembl Gene

ENSMUSG00000015222

Gene Name

microtubule-associated protein 2

Synonyms

G1-397-34, MAP-2, Mtap2, repro4

MMRRC Submission

045187-MU

Accession Numbers

Essential gene?

Possibly essential (E-score: 0.679) question?

Stock #

R7094 (G1)

Quality Score

225.009

Status

Validated

Chromosome

Chromosomal Location

66214432-66481742 bp(+) (GRCm39)

Type of Mutation

nonsense

DNA Base Change (assembly)

G to T at 66451886 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Glutamic Acid to Stop codon at position 259 (E259*)

Ref Sequence

ENSEMBL: ENSMUSP00000109646 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000024639] [ENSMUST00000077355] [ENSMUST00000114012] [ENSMUST00000114013] [ENSMUST00000114015] [ENSMUST00000114017] [ENSMUST00000114018] [ENSMUST00000145419] [ENSMUST00000172886] [ENSMUST00000173778] [ENSMUST00000173800] [ENSMUST00000173855]

AlphaFold

P20357

Predicted Effect

probably benign
Transcript: ENSMUST00000024639

SMART Domains

Protein: ENSMUSP00000024639
Gene: ENSMUSG00000015222

Domain	Start	End	E-Value	Type
low complexity region	120	141	N/A	INTRINSIC
low complexity region	182	196	N/A	INTRINSIC
low complexity region	206	222	N/A	INTRINSIC
low complexity region	229	251	N/A	INTRINSIC
Pfam:Tubulin-binding	300	331	2.1e-18	PFAM
Pfam:Tubulin-binding	332	362	9.1e-20	PFAM
Pfam:Tubulin-binding	363	394	1.7e-17	PFAM
low complexity region	422	435	N/A	INTRINSIC

Predicted Effect

probably benign
Transcript: ENSMUST00000077355

SMART Domains

Protein: ENSMUSP00000076577
Gene: ENSMUSG00000015222

Domain	Start	End	E-Value	Type
low complexity region	120	141	N/A	INTRINSIC
low complexity region	182	196	N/A	INTRINSIC
low complexity region	206	222	N/A	INTRINSIC
low complexity region	229	251	N/A	INTRINSIC
Pfam:Tubulin-binding	300	331	2.1e-18	PFAM
Pfam:Tubulin-binding	332	362	9.1e-20	PFAM
Pfam:Tubulin-binding	363	394	1.7e-17	PFAM
low complexity region	422	435	N/A	INTRINSIC

Predicted Effect

probably benign
Transcript: ENSMUST00000114012

SMART Domains

Protein: ENSMUSP00000109645
Gene: ENSMUSG00000015222

Domain	Start	End	E-Value	Type
low complexity region	133	140	N/A	INTRINSIC
low complexity region	181	195	N/A	INTRINSIC
low complexity region	205	221	N/A	INTRINSIC
low complexity region	228	250	N/A	INTRINSIC
Pfam:Tubulin-binding	299	330	2.1e-18	PFAM
Pfam:Tubulin-binding	331	361	9.1e-20	PFAM
Pfam:Tubulin-binding	362	393	1.7e-17	PFAM
low complexity region	421	434	N/A	INTRINSIC

Predicted Effect

probably null
Transcript: ENSMUST00000114013
AA Change: E259*

SMART Domains

Protein: ENSMUSP00000109646
Gene: ENSMUSG00000015222
AA Change: E259*

Domain	Start	End	E-Value	Type
Pfam:RII_binding_1	86	103	1.2e-5	PFAM
low complexity region	120	141	N/A	INTRINSIC
Pfam:MAP2_projctn	376	1510	N/A	PFAM
low complexity region	1543	1557	N/A	INTRINSIC
low complexity region	1567	1583	N/A	INTRINSIC
low complexity region	1590	1612	N/A	INTRINSIC
Pfam:Tubulin-binding	1662	1692	1.7e-13	PFAM
Pfam:Tubulin-binding	1693	1723	5.8e-18	PFAM
Pfam:Tubulin-binding	1724	1755	5.9e-18	PFAM
low complexity region	1783	1796	N/A	INTRINSIC

Predicted Effect

probably benign
Transcript: ENSMUST00000114015

SMART Domains

Protein: ENSMUSP00000109648
Gene: ENSMUSG00000015222

Domain	Start	End	E-Value	Type
low complexity region	120	141	N/A	INTRINSIC
low complexity region	182	196	N/A	INTRINSIC
low complexity region	206	222	N/A	INTRINSIC
low complexity region	229	251	N/A	INTRINSIC
Pfam:Tubulin-binding	300	331	2.1e-18	PFAM
Pfam:Tubulin-binding	332	362	9.1e-20	PFAM
Pfam:Tubulin-binding	363	394	1.7e-17	PFAM
low complexity region	422	435	N/A	INTRINSIC

Predicted Effect

probably benign
Transcript: ENSMUST00000114017

SMART Domains

Protein: ENSMUSP00000109650
Gene: ENSMUSG00000015222

Domain	Start	End	E-Value	Type
low complexity region	120	141	N/A	INTRINSIC
low complexity region	182	196	N/A	INTRINSIC
low complexity region	206	222	N/A	INTRINSIC
low complexity region	229	251	N/A	INTRINSIC
Pfam:Tubulin-binding	300	331	2.3e-18	PFAM
Pfam:Tubulin-binding	332	362	2.3e-19	PFAM
Pfam:Tubulin-binding	363	393	9.9e-20	PFAM
Pfam:Tubulin-binding	394	425	1.9e-17	PFAM
low complexity region	453	466	N/A	INTRINSIC

Predicted Effect

probably benign
Transcript: ENSMUST00000114018

SMART Domains

Protein: ENSMUSP00000109651
Gene: ENSMUSG00000015222

Domain	Start	End	E-Value	Type
low complexity region	120	141	N/A	INTRINSIC
low complexity region	182	196	N/A	INTRINSIC
low complexity region	206	222	N/A	INTRINSIC
low complexity region	229	251	N/A	INTRINSIC
Pfam:Tubulin-binding	300	331	2.3e-18	PFAM
Pfam:Tubulin-binding	332	362	2.3e-19	PFAM
Pfam:Tubulin-binding	363	393	9.9e-20	PFAM
Pfam:Tubulin-binding	394	425	1.9e-17	PFAM
low complexity region	453	466	N/A	INTRINSIC

Predicted Effect

probably null
Transcript: ENSMUST00000141148
AA Change: E194*

SMART Domains

Protein: ENSMUSP00000117996
Gene: ENSMUSG00000015222
AA Change: E194*

Domain	Start	End	E-Value	Type
Pfam:RII_binding_1	23	40	3.2e-6	PFAM
low complexity region	70	77	N/A	INTRINSIC

Predicted Effect

probably null
Transcript: ENSMUST00000145419
AA Change: E101*

SMART Domains

Protein: ENSMUSP00000134538
Gene: ENSMUSG00000015222
AA Change: E101*

Domain	Start	End	E-Value	Type
Pfam:MAP2_projctn	218	608	1.7e-250	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000172886

SMART Domains

Protein: ENSMUSP00000133446
Gene: ENSMUSG00000015222

Domain	Start	End	E-Value	Type
Pfam:MAP2_projctn	1	107	3.9e-54	PFAM
low complexity region	112	126	N/A	INTRINSIC
low complexity region	136	152	N/A	INTRINSIC
low complexity region	159	181	N/A	INTRINSIC
Pfam:Tubulin-binding	230	261	1.4e-18	PFAM
Pfam:Tubulin-binding	262	292	1.4e-19	PFAM
Pfam:Tubulin-binding	293	323	6e-20	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000173778

SMART Domains

Protein: ENSMUSP00000134651
Gene: ENSMUSG00000015222

Domain	Start	End	E-Value	Type
Pfam:MAP2_projctn	1	124	4.8e-84	PFAM
low complexity region	157	171	N/A	INTRINSIC
low complexity region	181	197	N/A	INTRINSIC
low complexity region	204	226	N/A	INTRINSIC
Pfam:Tubulin-binding	275	306	1.6e-18	PFAM
Pfam:Tubulin-binding	307	337	1.6e-19	PFAM
Pfam:Tubulin-binding	338	368	7e-20	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000173800

SMART Domains

Protein: ENSMUSP00000134518
Gene: ENSMUSG00000015222

Domain	Start	End	E-Value	Type
Pfam:MAP2_projctn	1	23	2.2e-11	PFAM
low complexity region	55	69	N/A	INTRINSIC
low complexity region	79	95	N/A	INTRINSIC
low complexity region	102	124	N/A	INTRINSIC
Pfam:Tubulin-binding	173	204	8.7e-19	PFAM
Pfam:Tubulin-binding	205	235	3.8e-20	PFAM

Predicted Effect

probably null
Transcript: ENSMUST00000173855
AA Change: E341*

SMART Domains

Protein: ENSMUSP00000134471
Gene: ENSMUSG00000015222
AA Change: E341*

Domain	Start	End	E-Value	Type
low complexity region	120	141	N/A	INTRINSIC
low complexity region	142	163	N/A	INTRINSIC
Pfam:MAP2_projctn	458	565	1.1e-52	PFAM

Meta Mutation Damage Score

0.9754

Coding Region Coverage

1x: 100.0%
3x: 100.0%
10x: 99.7%
20x: 99.1%

Validation Efficiency

97% (59/61)

MGI Phenotype

FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] This gene encodes a protein that belongs to the microtubule-associated protein family. The proteins of this family are thought to be involved in microtubule assembly, which is an essential step in neurogenesis. The products of similar genes in rat and mouse are neuron-specific cytoskeletal proteins that are enriched in dentrites, implicating a role in determining and stabilizing dentritic shape during neuron development. A number of alternatively spliced variants encoding distinct isoforms have been described. [provided by RefSeq, Jan 2010]
PHENOTYPE: Mice homozygous for a knock-out allele exhibit altered contextual memory. Mice homozygous for another knock-out allele display decreased body weight, altered microtubule density and organization in Purkinje cell dendrites, and reduced dendritic length inhippocampal neurons. [provided by MGI curators]

Allele List at MGI

Other mutations in this stock

Total: 60 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Abca13	A	T	11: 9,248,610 (GRCm39)	I2786F	probably damaging	Het
Actl6a	A	T	3: 32,760,487 (GRCm39)		probably benign	Het
Actn2	A	G	13: 12,324,543 (GRCm39)	V100A	probably damaging	Het
Arfgef3	G	T	10: 18,522,187 (GRCm39)	A613E	probably damaging	Het
Atf6b	G	A	17: 34,872,790 (GRCm39)		probably null	Het
Bub1	T	A	2: 127,663,681 (GRCm39)	E240V	probably null	Het
C1ra	G	A	6: 124,494,684 (GRCm39)	E316K	probably benign	Het
Ccdc141	C	A	2: 76,871,797 (GRCm39)	R829L	possibly damaging	Het
Ccdc85c	GCCGCCGCCGCCAGCGCCCCCCGCCGCCGCCAGCGCC	GCCGCCGCCGCCAGCGCC	12: 108,240,877 (GRCm39)		probably null	Het
Cd209g	T	C	8: 4,186,790 (GRCm39)	F112L	possibly damaging	Het
Cebpe	C	T	14: 54,948,060 (GRCm39)	R261H	probably damaging	Het
Cfap100	T	C	6: 90,390,436 (GRCm39)	E68G		Het
Chd9	A	G	8: 91,716,189 (GRCm39)	N921S	unknown	Het
Chil6	T	C	3: 106,311,486 (GRCm39)	N98S	probably damaging	Het
Clip1	T	C	5: 123,761,333 (GRCm39)	K734E	probably benign	Het
Cracd	C	T	5: 77,006,879 (GRCm39)	P1080L	unknown	Het
Cyp11b2	A	G	15: 74,725,507 (GRCm39)	F204S	possibly damaging	Het
Dnah5	A	G	15: 28,453,482 (GRCm39)	T4418A	probably damaging	Het
Dysf	T	C	6: 84,077,184 (GRCm39)	V649A	probably benign	Het
Ergic3	G	A	2: 155,858,683 (GRCm39)	V270M	possibly damaging	Het
Eva1a	C	T	6: 82,069,024 (GRCm39)	T117I	probably damaging	Het
Fat4	G	A	3: 38,944,023 (GRCm39)	G972D	probably damaging	Het
Gm14412	A	T	2: 177,009,138 (GRCm39)	N39K	probably damaging	Het
Gm5565	T	C	5: 146,095,084 (GRCm39)	T221A	probably benign	Het
Gnptab	T	G	10: 88,215,366 (GRCm39)	V29G	possibly damaging	Het
Grem2	T	C	1: 174,664,555 (GRCm39)	Y98C	probably damaging	Het
Grik2	A	T	10: 49,232,012 (GRCm39)	I506N	possibly damaging	Het
Has2	T	A	15: 56,545,017 (GRCm39)	Y195F	probably damaging	Het
Kics2	A	G	10: 121,576,098 (GRCm39)	Y73C	possibly damaging	Het
Lars2	T	C	9: 123,288,650 (GRCm39)	L832P	probably damaging	Het
Lipo5	T	A	19: 33,446,249 (GRCm39)	E49D	probably damaging	Het
Macc1	T	A	12: 119,414,126 (GRCm39)	Y767*	probably null	Het
Mcm9	T	C	10: 53,496,253 (GRCm39)	D310G	probably damaging	Het
Mink1	C	A	11: 70,500,901 (GRCm39)		probably null	Het
Mtrr	T	C	13: 68,727,803 (GRCm39)	T48A	possibly damaging	Het
Nrsn1	A	T	13: 25,437,724 (GRCm39)	I68N	possibly damaging	Het
Or1ab2	A	T	8: 72,863,347 (GRCm39)		probably benign	Het
Or4a71	A	T	2: 89,357,902 (GRCm39)	I284K	probably damaging	Het
Or51f23b	A	G	7: 102,402,305 (GRCm39)	M277T	probably benign	Het
Or5p60	G	T	7: 107,723,840 (GRCm39)	T210N	probably benign	Het
Or8k36-ps1	T	C	2: 86,437,672 (GRCm39)	N81S	unknown	Het
Pcdh17	A	G	14: 84,684,835 (GRCm39)	D434G	probably damaging	Het
Rnf213	T	A	11: 119,328,430 (GRCm39)		probably null	Het
Selplg	GTCTGCCTCCATGGGTGCTGGCTGCGAGGTCTCTGCCTCCATGGGTGCTGGCTGCGAGGTCTCTGCCTCCATGGGTGCTGGCTGCGAGGTCTCTGCCTCCATGGGTGCTGGCTGCGAGGTCTCT	GTCTGCCTCCATGGGTGCTGGCTGCGAGGTCTCTGCCTCCATGGGTGCTGGCTGCGAGGTCTCTGCCTCCATGGGTGCTGGCTGCGAGGTCTCT	5: 113,957,756 (GRCm39)		probably benign	Het
Sez6l2	A	G	7: 126,552,096 (GRCm39)	E121G	probably damaging	Het
Slc35e4	T	C	11: 3,863,118 (GRCm39)	S24G	probably benign	Het
Slc39a2	G	T	14: 52,131,146 (GRCm39)		probably benign	Het
Slitrk5	C	T	14: 111,918,268 (GRCm39)	P631S	probably benign	Het
Taf2	A	G	15: 54,923,482 (GRCm39)	V265A	probably benign	Het
Tas2r106	T	C	6: 131,655,542 (GRCm39)	N103S	probably benign	Het
Tgm1	T	C	14: 55,942,300 (GRCm39)	T684A	possibly damaging	Het
Tgm7	C	T	2: 120,929,489 (GRCm39)	G262S	probably damaging	Het
Tpp2	T	A	1: 44,008,148 (GRCm39)	S451T	probably damaging	Het
Trim15	T	C	17: 37,173,788 (GRCm39)	Y240C	probably benign	Het
Trio	A	T	15: 27,891,534 (GRCm39)	C465S	unknown	Het
Ttc22	G	A	4: 106,493,104 (GRCm39)	W250*	probably null	Het
Upb1	C	A	10: 75,274,042 (GRCm39)	F356L	probably damaging	Het
Vmn2r99	T	A	17: 19,599,573 (GRCm39)	M419K	probably benign	Het
Vstm2a	G	A	11: 16,207,990 (GRCm39)		probably benign	Het
Zfp820	T	C	17: 22,038,246 (GRCm39)	T361A	probably benign	Het

Other mutations in Map2

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01518:Map2	APN	1	66,464,490 (GRCm39)	missense	probably damaging	1.00
IGL02135:Map2	APN	1	66,419,920 (GRCm39)	nonsense	probably null
IGL02526:Map2	APN	1	66,419,876 (GRCm39)	missense	possibly damaging	0.94
Annas	UTSW	1	66,472,756 (GRCm39)	critical splice donor site	probably null
calliope	UTSW	1	66,464,457 (GRCm39)	missense	probably damaging	1.00
carthage	UTSW	1	66,453,807 (GRCm39)	missense	probably damaging	1.00
costas	UTSW	1	66,451,812 (GRCm39)	missense	possibly damaging	0.94
Jacobin	UTSW	1	66,438,578 (GRCm39)	missense	probably damaging	1.00
Nectar	UTSW	1	66,454,522 (GRCm39)	nonsense	probably null
ruby_throat	UTSW	1	66,454,043 (GRCm39)	missense	possibly damaging	0.67
Rufous	UTSW	1	66,419,927 (GRCm39)	missense	probably damaging	1.00
Speckled	UTSW	1	66,451,886 (GRCm39)	nonsense	probably null
Sunbird	UTSW	1	66,454,646 (GRCm39)	missense	probably benign	0.01
swift	UTSW	1	66,455,523 (GRCm39)	missense	probably damaging	1.00
E0370:Map2	UTSW	1	66,455,883 (GRCm39)	unclassified	probably benign
PIT4362001:Map2	UTSW	1	66,451,677 (GRCm39)	missense	probably benign	0.05
R0067:Map2	UTSW	1	66,452,322 (GRCm39)	missense	probably benign	0.04
R0238:Map2	UTSW	1	66,455,265 (GRCm39)	missense	probably damaging	1.00
R0238:Map2	UTSW	1	66,455,265 (GRCm39)	missense	probably damaging	1.00
R0239:Map2	UTSW	1	66,455,265 (GRCm39)	missense	probably damaging	1.00
R0239:Map2	UTSW	1	66,455,265 (GRCm39)	missense	probably damaging	1.00
R0268:Map2	UTSW	1	66,419,881 (GRCm39)	nonsense	probably null
R0302:Map2	UTSW	1	66,453,987 (GRCm39)	missense	probably benign	0.15
R0305:Map2	UTSW	1	66,452,253 (GRCm39)	missense	probably benign	0.00
R0409:Map2	UTSW	1	66,472,739 (GRCm39)	missense	probably damaging	1.00
R0561:Map2	UTSW	1	66,464,656 (GRCm39)	missense	probably damaging	1.00
R0674:Map2	UTSW	1	66,452,361 (GRCm39)	missense	probably damaging	1.00
R0738:Map2	UTSW	1	66,464,348 (GRCm39)	splice site	probably benign
R0893:Map2	UTSW	1	66,419,927 (GRCm39)	missense	probably damaging	1.00
R1305:Map2	UTSW	1	66,464,554 (GRCm39)	missense	probably damaging	1.00
R1534:Map2	UTSW	1	66,452,339 (GRCm39)	missense	probably benign	0.33
R1632:Map2	UTSW	1	66,454,245 (GRCm39)	missense	possibly damaging	0.60
R1682:Map2	UTSW	1	66,454,781 (GRCm39)	splice site	probably null
R1774:Map2	UTSW	1	66,453,233 (GRCm39)	missense	probably damaging	1.00
R2014:Map2	UTSW	1	66,455,295 (GRCm39)	missense	possibly damaging	0.55
R2017:Map2	UTSW	1	66,451,958 (GRCm39)	missense	probably damaging	1.00
R2050:Map2	UTSW	1	66,453,473 (GRCm39)	missense	probably damaging	0.98
R2093:Map2	UTSW	1	66,438,599 (GRCm39)	missense	probably damaging	1.00
R2214:Map2	UTSW	1	66,459,345 (GRCm39)	missense	probably damaging	0.99
R2284:Map2	UTSW	1	66,453,227 (GRCm39)	missense	probably damaging	1.00
R3011:Map2	UTSW	1	66,453,771 (GRCm39)	missense	probably damaging	1.00
R3105:Map2	UTSW	1	66,472,756 (GRCm39)	critical splice donor site	probably null
R3708:Map2	UTSW	1	66,455,714 (GRCm39)	unclassified	probably benign
R3709:Map2	UTSW	1	66,455,015 (GRCm39)	nonsense	probably null
R3729:Map2	UTSW	1	66,451,605 (GRCm39)	missense	possibly damaging	0.80
R3760:Map2	UTSW	1	66,478,077 (GRCm39)	missense	probably damaging	1.00
R3788:Map2	UTSW	1	66,456,022 (GRCm39)	missense	probably damaging	0.99
R3789:Map2	UTSW	1	66,456,022 (GRCm39)	missense	probably damaging	0.99
R4003:Map2	UTSW	1	66,454,899 (GRCm39)	missense	probably damaging	1.00
R4120:Map2	UTSW	1	66,455,063 (GRCm39)	missense	probably damaging	1.00
R4172:Map2	UTSW	1	66,452,759 (GRCm39)	missense	possibly damaging	0.89
R4198:Map2	UTSW	1	66,464,457 (GRCm39)	missense	probably damaging	1.00
R4200:Map2	UTSW	1	66,464,457 (GRCm39)	missense	probably damaging	1.00
R4205:Map2	UTSW	1	66,464,449 (GRCm39)	missense	probably damaging	1.00
R4613:Map2	UTSW	1	66,464,628 (GRCm39)	missense	probably damaging	1.00
R4700:Map2	UTSW	1	66,449,796 (GRCm39)	missense	probably damaging	0.96
R4974:Map2	UTSW	1	66,452,664 (GRCm39)	missense	probably benign	0.15
R5007:Map2	UTSW	1	66,452,448 (GRCm39)	missense	possibly damaging	0.86
R5039:Map2	UTSW	1	66,477,955 (GRCm39)	missense	probably damaging	1.00
R5237:Map2	UTSW	1	66,478,169 (GRCm39)	unclassified	probably benign
R5313:Map2	UTSW	1	66,464,538 (GRCm39)	missense	probably damaging	1.00
R5455:Map2	UTSW	1	66,438,550 (GRCm39)	missense	probably damaging	1.00
R5490:Map2	UTSW	1	66,452,292 (GRCm39)	missense	probably damaging	1.00
R5517:Map2	UTSW	1	66,454,415 (GRCm39)	missense	probably benign	0.00
R5532:Map2	UTSW	1	66,453,779 (GRCm39)	missense	probably damaging	1.00
R5583:Map2	UTSW	1	66,455,196 (GRCm39)	missense	probably damaging	1.00
R5764:Map2	UTSW	1	66,454,034 (GRCm39)	missense	probably damaging	0.99
R5996:Map2	UTSW	1	66,454,043 (GRCm39)	missense	possibly damaging	0.67
R6058:Map2	UTSW	1	66,454,573 (GRCm39)	missense	probably benign	0.05
R6199:Map2	UTSW	1	66,464,637 (GRCm39)	missense	probably damaging	1.00
R6208:Map2	UTSW	1	66,470,749 (GRCm39)	missense	probably damaging	1.00
R6276:Map2	UTSW	1	66,438,578 (GRCm39)	missense	probably damaging	1.00
R6378:Map2	UTSW	1	66,454,488 (GRCm39)	missense	probably damaging	1.00
R6424:Map2	UTSW	1	66,453,946 (GRCm39)	missense	possibly damaging	0.67
R6743:Map2	UTSW	1	66,454,766 (GRCm39)	missense	probably benign	0.04
R6837:Map2	UTSW	1	66,453,731 (GRCm39)	missense	probably damaging	1.00
R6901:Map2	UTSW	1	66,460,932 (GRCm39)	missense	possibly damaging	0.94
R6984:Map2	UTSW	1	66,454,395 (GRCm39)	missense	possibly damaging	0.90
R6989:Map2	UTSW	1	66,454,065 (GRCm39)	missense	probably benign	0.00
R7001:Map2	UTSW	1	66,454,646 (GRCm39)	missense	probably benign	0.01
R7055:Map2	UTSW	1	66,455,983 (GRCm39)	missense	probably damaging	1.00
R7106:Map2	UTSW	1	66,449,903 (GRCm39)	missense	possibly damaging	0.92
R7182:Map2	UTSW	1	66,451,812 (GRCm39)	missense	possibly damaging	0.94
R7235:Map2	UTSW	1	66,453,807 (GRCm39)	missense	probably damaging	1.00
R7424:Map2	UTSW	1	66,453,983 (GRCm39)	missense	possibly damaging	0.69
R7473:Map2	UTSW	1	66,454,617 (GRCm39)	missense	probably damaging	1.00
R7642:Map2	UTSW	1	66,452,466 (GRCm39)	missense	probably benign	0.01
R7660:Map2	UTSW	1	66,453,536 (GRCm39)	missense	probably damaging	1.00
R7673:Map2	UTSW	1	66,452,933 (GRCm39)	missense	probably benign	0.03
R7768:Map2	UTSW	1	66,453,642 (GRCm39)	missense	possibly damaging	0.94
R7796:Map2	UTSW	1	66,455,654 (GRCm39)	splice site	probably null
R7834:Map2	UTSW	1	66,455,647 (GRCm39)	missense	probably damaging	1.00
R7842:Map2	UTSW	1	66,455,570 (GRCm39)	missense	probably benign
R7955:Map2	UTSW	1	66,452,875 (GRCm39)	missense	probably damaging	1.00
R8056:Map2	UTSW	1	66,454,779 (GRCm39)	missense	probably damaging	0.99
R8118:Map2	UTSW	1	66,464,550 (GRCm39)	missense	probably damaging	1.00
R8135:Map2	UTSW	1	66,452,828 (GRCm39)	missense	probably damaging	1.00
R8152:Map2	UTSW	1	66,453,902 (GRCm39)	missense	probably benign	0.00
R8223:Map2	UTSW	1	66,464,649 (GRCm39)	missense	probably damaging	1.00
R8329:Map2	UTSW	1	66,454,272 (GRCm39)	missense	probably benign	0.01
R8344:Map2	UTSW	1	66,460,872 (GRCm39)	missense	probably damaging	1.00
R8475:Map2	UTSW	1	66,453,164 (GRCm39)	missense	probably damaging	1.00
R8548:Map2	UTSW	1	66,452,499 (GRCm39)	missense	probably damaging	1.00
R8680:Map2	UTSW	1	66,460,872 (GRCm39)	missense	probably damaging	1.00
R8713:Map2	UTSW	1	66,453,781 (GRCm39)	missense	probably damaging	1.00
R8745:Map2	UTSW	1	66,452,556 (GRCm39)	missense	probably benign	0.00
R8786:Map2	UTSW	1	66,472,755 (GRCm39)	critical splice donor site	probably benign
R8790:Map2	UTSW	1	66,477,997 (GRCm39)	missense	probably damaging	1.00
R8874:Map2	UTSW	1	66,455,523 (GRCm39)	missense	probably damaging	1.00
R8887:Map2	UTSW	1	66,454,758 (GRCm39)	missense	possibly damaging	0.48
R8948:Map2	UTSW	1	66,419,684 (GRCm39)	missense	probably damaging	1.00
R8950:Map2	UTSW	1	66,419,684 (GRCm39)	missense	probably damaging	1.00
R8998:Map2	UTSW	1	66,452,473 (GRCm39)	missense	possibly damaging	0.91
R8999:Map2	UTSW	1	66,452,473 (GRCm39)	missense	possibly damaging	0.91
R9072:Map2	UTSW	1	66,453,312 (GRCm39)	missense	probably damaging	1.00
R9088:Map2	UTSW	1	66,453,773 (GRCm39)	missense	probably damaging	1.00
R9089:Map2	UTSW	1	66,452,098 (GRCm39)	missense	probably benign	0.09
R9106:Map2	UTSW	1	66,454,522 (GRCm39)	nonsense	probably null
R9112:Map2	UTSW	1	66,472,723 (GRCm39)	nonsense	probably null
R9120:Map2	UTSW	1	66,453,218 (GRCm39)	missense	probably damaging	1.00
R9161:Map2	UTSW	1	66,477,503 (GRCm39)	missense	possibly damaging	0.65
R9464:Map2	UTSW	1	66,454,497 (GRCm39)	missense	probably damaging	1.00
R9589:Map2	UTSW	1	66,449,753 (GRCm39)	missense	probably benign
V8831:Map2	UTSW	1	66,455,004 (GRCm39)	missense	probably damaging	1.00
Z1177:Map2	UTSW	1	66,477,520 (GRCm39)	missense	probably damaging	0.96
Z1177:Map2	UTSW	1	66,419,839 (GRCm39)	missense	probably benign	0.01

Predicted Primers

PCR Primer
(F):5'- TGAAGACTTTGAACATGCTGCC -3'
(R):5'- GAAGAGTGAAACTTCCACCATG -3'

Sequencing Primer
(F):5'- GCTGCCTTAGTTCCTGACACAAG -3'
(R):5'- TCCACCATGGAAGGGGCTTG -3'

Posted On

2019-05-15