Mutagenetix > Incidental Mutation

Incidental Mutation 'R7099:Zfp352'

550686

Institutional Source

Beutler Lab

Gene Symbol

Zfp352

Ensembl Gene

ENSMUSG00000070902

Gene Name

zinc finger protein 352

Synonyms

2czf48

MMRRC Submission

045191-MU

Accession Numbers

Essential gene?

Non essential (E-score: 0.000) question?

Stock #

R7099 (G1)

Quality Score

225.009

Status

Validated

Chromosome

Chromosomal Location

90218820-90225702 bp(+) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

A to G at 90224880 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Lysine to Arginine at position 419 (K419R)

Ref Sequence

ENSEMBL: ENSMUSP00000102746 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000080541] [ENSMUST00000107129]

AlphaFold

A2AML7

Predicted Effect

probably benign
Transcript: ENSMUST00000080541
AA Change: K419R

PolyPhen 2 Score 0.004 (Sensitivity: 0.98; Specificity: 0.59)

SMART Domains

Protein: ENSMUSP00000079383
Gene: ENSMUSG00000070902
AA Change: K419R

Domain	Start	End	E-Value	Type
ZnF_C2H2	459	483	3.34e-2	SMART
ZnF_C2H2	489	513	8.22e-2	SMART
ZnF_C2H2	519	542	1.76e-1	SMART

Predicted Effect

probably benign
Transcript: ENSMUST00000107129
AA Change: K419R

PolyPhen 2 Score 0.004 (Sensitivity: 0.98; Specificity: 0.59)

SMART Domains

Protein: ENSMUSP00000102746
Gene: ENSMUSG00000070902
AA Change: K419R

Domain	Start	End	E-Value	Type
ZnF_C2H2	459	483	3.34e-2	SMART
ZnF_C2H2	489	513	8.22e-2	SMART
ZnF_C2H2	519	542	1.76e-1	SMART

Coding Region Coverage

1x: 100.0%
3x: 100.0%
10x: 99.8%
20x: 99.3%

Validation Efficiency

100% (65/65)

Allele List at MGI

Other mutations in this stock

Total: 66 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
4930453N24Rik	C	T	16: 64,770,788	A26T	probably benign	Het
Acly	T	C	11: 100,492,291		probably null	Het
Adam29	A	C	8: 55,871,404	L672V	probably benign	Het
Adgrf1	T	C	17: 43,310,602	S577P	probably benign	Het
Ankar	T	C	1: 72,643,293	K1371R	probably damaging	Het
Arid5b	T	C	10: 68,098,179	D631G	probably damaging	Het
Brpf3	T	C	17: 28,806,637	V228A	probably benign	Het
C3	T	C	17: 57,206,276	D1457G	probably benign	Het
Calr4	A	T	4: 109,242,229	N143I	probably benign	Het
Catsperd	T	G	17: 56,628,811		probably null	Het
Cobl	T	A	11: 12,296,540	H154L		Het
Cryzl2	G	A	1: 157,488,584		probably benign	Het
Dennd1c	A	G	17: 57,067,915		probably null	Het
Dnah8	A	T	17: 30,704,724	D1222V	possibly damaging	Het
Errfi1	T	C	4: 150,866,768	S218P	probably benign	Het
Fbxw27	G	T	9: 109,770,155	T398N	probably damaging	Het
Fhod3	A	G	18: 25,090,162	D855G	probably benign	Het
Flii	A	G	11: 60,720,655	V410A	probably benign	Het
Fsip2	C	A	2: 82,987,624	P4567Q	probably benign	Het
Fxyd1	T	G	7: 31,053,033	Q66H	probably damaging	Het
Gdi1	G	A	X: 74,306,855	R55H	probably benign	Het
Gramd3	C	T	18: 56,491,945	T370I	probably benign	Het
Kdr	A	G	5: 75,944,333	V1079A	probably damaging	Het
Lmx1a	G	A	1: 167,830,546	G166D	probably damaging	Het
Lrrfip2	A	G	9: 111,173,108	R92G	probably benign	Het
Map1a	T	A	2: 121,300,517	S605T	probably benign	Het
Megf8	A	T	7: 25,346,520	D1496V	probably damaging	Het
Mgam	T	C	6: 40,661,716	V461A	probably benign	Het
Muc6	AGGCGCAGAAACCCTGGC	AGGC	7: 141,634,450		probably null	Het
Nav3	T	C	10: 109,703,334	T2069A	probably benign	Het
Nbeal2	A	T	9: 110,645,438		probably null	Het
Ndst1	A	G	18: 60,695,500	F661L	possibly damaging	Het
Neu3	G	A	7: 99,813,820	T232M	possibly damaging	Het
Nf1	T	C	11: 79,570,330	S741P	probably benign	Het
Nr5a1	G	T	2: 38,694,136	L424M	probably damaging	Het
Nuf2	G	A	1: 169,506,072	T345M	probably benign	Het
Olfr1301	A	T	2: 111,755,076	T276S	probably benign	Het
Olfr1426	A	G	19: 12,088,166	F209L	possibly damaging	Het
Olfr212	T	A	6: 116,516,760	*328R	probably null	Het
Olfr952	G	A	9: 39,426,303	T256I	probably benign	Het
Otud7a	A	G	7: 63,757,455	E502G	possibly damaging	Het
Otulin	A	G	15: 27,608,746	L237S	probably damaging	Het
Pias1	A	G	9: 62,881,145	M79T		Het
Prom2	T	C	2: 127,539,778	E206G	probably benign	Het
Scarb1	A	T	5: 125,304,350	N43K	probably damaging	Het
Sdad1	A	G	5: 92,293,973	V365A	possibly damaging	Het
Sdk2	T	C	11: 113,834,905	T1173A	probably damaging	Het
Sidt1	A	G	16: 44,243,497	S803P	probably damaging	Het
Slc45a1	A	T	4: 150,629,573	D738E	probably benign	Het
Slc4a7	T	A	14: 14,733,750	H53Q	probably damaging	Het
Spata22	T	C	11: 73,340,399	F160L	probably benign	Het
Stag1	G	A	9: 100,944,826	V949I	probably benign	Het
Syne1	T	C	10: 5,123,744	S1200G	probably benign	Het
Tbc1d9	A	G	8: 83,254,891	E729G	probably damaging	Het
Tcaf2	C	T	6: 42,630,341	M226I	probably benign	Het
Tep1	T	C	14: 50,844,487		probably null	Het
Tigd2	C	A	6: 59,210,181	T11K	probably damaging	Het
Trappc9	A	G	15: 72,693,619	V941A	probably benign	Het
Ugt2b37	A	G	5: 87,240,989	M455T	probably benign	Het
Usp42	A	T	5: 143,726,645	S95T	probably damaging	Het
Usp44	T	C	10: 93,850,187	I488T	possibly damaging	Het
Vmn1r73	T	C	7: 11,756,393	I46T	probably damaging	Het
Vmn2r34	A	T	7: 7,672,541	I616N	probably damaging	Het
Zfp595	T	A	13: 67,317,647	H187L	probably damaging	Het
Zfp804b	A	T	5: 6,772,161	S301T	probably benign	Het
Zzef1	T	C	11: 72,872,649	V1374A	possibly damaging	Het

Other mutations in Zfp352

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01963:Zfp352	APN	4	90224154	missense	possibly damaging	0.95
IGL02252:Zfp352	APN	4	90224130	missense	probably benign	0.02
IGL03156:Zfp352	APN	4	90224087	missense	possibly damaging	0.57
IGL03167:Zfp352	APN	4	90224702	missense	probably damaging	0.99
IGL03190:Zfp352	APN	4	90223757	missense	possibly damaging	0.94
IGL03335:Zfp352	APN	4	90224346	missense	probably damaging	0.99
R0051:Zfp352	UTSW	4	90224285	missense	probably damaging	0.99
R0403:Zfp352	UTSW	4	90225009	missense	possibly damaging	0.60
R0550:Zfp352	UTSW	4	90224690	missense	probably damaging	0.99
R0671:Zfp352	UTSW	4	90223919	missense	probably benign
R1034:Zfp352	UTSW	4	90224156	missense	possibly damaging	0.94
R1754:Zfp352	UTSW	4	90223809	missense	probably benign	0.23
R2016:Zfp352	UTSW	4	90225171	missense	probably benign	0.42
R2064:Zfp352	UTSW	4	90225120	missense	probably benign	0.08
R2308:Zfp352	UTSW	4	90225243	missense	probably benign	0.00
R3552:Zfp352	UTSW	4	90225102	missense	probably benign	0.33
R3794:Zfp352	UTSW	4	90225149	missense	probably damaging	1.00
R3795:Zfp352	UTSW	4	90225149	missense	probably damaging	1.00
R4135:Zfp352	UTSW	4	90225024	missense	probably damaging	0.96
R4356:Zfp352	UTSW	4	90223834	missense	possibly damaging	0.91
R4409:Zfp352	UTSW	4	90225164	missense	probably benign	0.00
R4590:Zfp352	UTSW	4	90224535	missense	probably damaging	0.98
R4614:Zfp352	UTSW	4	90225081	missense	probably benign	0.00
R4617:Zfp352	UTSW	4	90225081	missense	probably benign	0.00
R4618:Zfp352	UTSW	4	90225081	missense	probably benign	0.00
R4741:Zfp352	UTSW	4	90224940	missense	possibly damaging	0.94
R4931:Zfp352	UTSW	4	90224304	missense	probably damaging	0.98
R4959:Zfp352	UTSW	4	90224139	missense	probably benign	0.01
R4973:Zfp352	UTSW	4	90224139	missense	probably benign	0.01
R5167:Zfp352	UTSW	4	90224216	missense	possibly damaging	0.94
R5260:Zfp352	UTSW	4	90224460	missense	probably damaging	0.99
R5524:Zfp352	UTSW	4	90225104	missense	possibly damaging	0.95
R5942:Zfp352	UTSW	4	90225070	missense	probably damaging	0.98
R6802:Zfp352	UTSW	4	90225200	missense	probably benign	0.33
R6819:Zfp352	UTSW	4	90224699	missense	probably benign
R7072:Zfp352	UTSW	4	90224424	missense	probably benign	0.00
R7569:Zfp352	UTSW	4	90223659	missense	possibly damaging	0.77
R7645:Zfp352	UTSW	4	90224777	missense	probably benign	0.13
R7705:Zfp352	UTSW	4	90225275	missense	possibly damaging	0.94
R8424:Zfp352	UTSW	4	90224243	missense	possibly damaging	0.87
R9180:Zfp352	UTSW	4	90224881	missense	probably benign	0.38
R9378:Zfp352	UTSW	4	90224338	missense	probably benign	0.13
R9509:Zfp352	UTSW	4	90224706	missense	probably damaging	0.99
R9623:Zfp352	UTSW	4	90224891	missense	probably benign	0.00

Predicted Primers

PCR Primer
(F):5'- ACAACCTGGCTTGCCCTTTG -3'
(R):5'- AGGCTCATCACACCCGTATTTC -3'

Sequencing Primer
(F):5'- TGGAGACAAGTCCCACATCTG -3'
(R):5'- ACACCCGTATTTCCTCTTATCAGTG -3'

Posted On

2019-05-15