Mutagenetix > Incidental Mutations

Incidental Mutation 'R7102:Prg4'

550872

Institutional Source

Beutler Lab

Gene Symbol

Prg4

Ensembl Gene

ENSMUSG00000006014

Gene Name

proteoglycan 4 (megakaryocyte stimulating factor, articular superficial zone protein)

Synonyms

DOL54, SZP, lubricin, MSF

MMRRC Submission

Accession Numbers

Is this an essential gene?

Probably non essential (E-score: 0.180) question?

Stock #

R7102 (G1)

Quality Score

225.009

Status

Not validated

Chromosome

Chromosomal Location

150449412-150466165 bp(-) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

C to T at 150452254 bp

Zygosity

Heterozygous

Amino Acid Change

Cysteine to Tyrosine at position 220 (C220Y)

Ref Sequence

ENSEMBL: ENSMUSP00000006171 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000006171] [ENSMUST00000111901] [ENSMUST00000111902] [ENSMUST00000119161] [ENSMUST00000124484] [ENSMUST00000124973] [ENSMUST00000161320] [ENSMUST00000161611] [ENSMUST00000162367] [ENSMUST00000164600]

Predicted Effect

probably damaging
Transcript: ENSMUST00000006171
AA Change: C220Y

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)

SMART Domains

Protein: ENSMUSP00000006171
Gene: ENSMUSG00000006014
AA Change: C220Y

Domain	Start	End	E-Value	Type
signal peptide	1	24	N/A	INTRINSIC
SO	26	68	9.21e-18	SMART
SO	69	108	4.21e-12	SMART
low complexity region	110	124	N/A	INTRINSIC
HX	169	211	1.67e-7	SMART
HX	213	256	3.76e-10	SMART
Blast:HX	369	422	2e-27	BLAST

Predicted Effect

unknown
Transcript: ENSMUST00000111901
AA Change: C762Y

SMART Domains

Protein: ENSMUSP00000107532
Gene: ENSMUSG00000006014
AA Change: C762Y

Domain	Start	End	E-Value	Type
signal peptide	1	24	N/A	INTRINSIC
SO	26	68	9.21e-18	SMART
SO	69	108	4.21e-12	SMART
low complexity region	110	124	N/A	INTRINSIC
low complexity region	141	151	N/A	INTRINSIC
low complexity region	189	221	N/A	INTRINSIC
low complexity region	241	535	N/A	INTRINSIC
low complexity region	570	623	N/A	INTRINSIC
HX	711	753	1.67e-7	SMART
HX	755	798	3.76e-10	SMART
Blast:HX	911	964	5e-27	BLAST

Predicted Effect

probably damaging
Transcript: ENSMUST00000111902
AA Change: C809Y

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)

SMART Domains

Protein: ENSMUSP00000107533
Gene: ENSMUSG00000006014
AA Change: C809Y

Domain	Start	End	E-Value	Type
signal peptide	1	24	N/A	INTRINSIC
SO	25	67	2.71e-15	SMART
low complexity region	69	81	N/A	INTRINSIC
low complexity region	86	143	N/A	INTRINSIC
low complexity region	150	171	N/A	INTRINSIC
low complexity region	188	198	N/A	INTRINSIC
low complexity region	236	268	N/A	INTRINSIC
low complexity region	288	582	N/A	INTRINSIC
internal_repeat_1	588	614	2.72e-5	PROSPERO
low complexity region	617	670	N/A	INTRINSIC
internal_repeat_1	680	706	2.72e-5	PROSPERO
HX	758	800	1.67e-7	SMART
HX	802	845	3.76e-10	SMART
Blast:HX	958	1011	4e-27	BLAST

Predicted Effect

probably benign
Transcript: ENSMUST00000119161

SMART Domains

Protein: ENSMUSP00000112606
Gene: ENSMUSG00000006005

Domain	Start	End	E-Value	Type
coiled coil region	49	370	N/A	INTRINSIC
coiled coil region	423	515	N/A	INTRINSIC
low complexity region	518	534	N/A	INTRINSIC
coiled coil region	539	604	N/A	INTRINSIC
low complexity region	690	703	N/A	INTRINSIC
low complexity region	782	795	N/A	INTRINSIC
low complexity region	811	826	N/A	INTRINSIC
low complexity region	1003	1019	N/A	INTRINSIC
Pfam:TPR_MLP1_2	1036	1167	9.1e-33	PFAM
coiled coil region	1215	1421	N/A	INTRINSIC
coiled coil region	1473	1629	N/A	INTRINSIC
internal_repeat_3	1630	1691	1.48e-5	PROSPERO
low complexity region	1695	1717	N/A	INTRINSIC
low complexity region	1761	1777	N/A	INTRINSIC
internal_repeat_5	1814	1827	5.58e-5	PROSPERO
internal_repeat_3	1819	1881	1.48e-5	PROSPERO
internal_repeat_4	1875	1895	5.58e-5	PROSPERO
internal_repeat_1	1893	1919	2.03e-6	PROSPERO
low complexity region	1920	1933	N/A	INTRINSIC
low complexity region	1942	1981	N/A	INTRINSIC
low complexity region	1989	2014	N/A	INTRINSIC
internal_repeat_4	2017	2036	5.58e-5	PROSPERO
low complexity region	2059	2078	N/A	INTRINSIC
internal_repeat_2	2084	2135	3.95e-6	PROSPERO
internal_repeat_5	2127	2140	5.58e-5	PROSPERO
internal_repeat_1	2154	2179	2.03e-6	PROSPERO
internal_repeat_2	2156	2212	3.95e-6	PROSPERO
low complexity region	2239	2251	N/A	INTRINSIC
low complexity region	2263	2277	N/A	INTRINSIC
low complexity region	2292	2314	N/A	INTRINSIC
low complexity region	2346	2357	N/A	INTRINSIC

Predicted Effect

probably benign
Transcript: ENSMUST00000124484

SMART Domains

Protein: ENSMUSP00000121991
Gene: ENSMUSG00000006005

Domain	Start	End	E-Value	Type
low complexity region	26	38	N/A	INTRINSIC
low complexity region	50	64	N/A	INTRINSIC
low complexity region	79	101	N/A	INTRINSIC
low complexity region	133	144	N/A	INTRINSIC

Predicted Effect

probably benign
Transcript: ENSMUST00000124973

SMART Domains

Protein: ENSMUSP00000117616
Gene: ENSMUSG00000006005

Domain	Start	End	E-Value	Type
low complexity region	3	14	N/A	INTRINSIC
low complexity region	24	77	N/A	INTRINSIC
coiled coil region	123	444	N/A	INTRINSIC
coiled coil region	497	589	N/A	INTRINSIC
low complexity region	592	608	N/A	INTRINSIC
coiled coil region	613	678	N/A	INTRINSIC
low complexity region	764	777	N/A	INTRINSIC
low complexity region	856	869	N/A	INTRINSIC
low complexity region	885	900	N/A	INTRINSIC
low complexity region	1077	1093	N/A	INTRINSIC
Pfam:TPR_MLP1_2	1112	1240	5.1e-37	PFAM
coiled coil region	1289	1495	N/A	INTRINSIC
low complexity region	1682	1698	N/A	INTRINSIC
internal_repeat_5	1703	1750	8.04e-5	PROSPERO
internal_repeat_3	1704	1765	1.07e-5	PROSPERO
low complexity region	1769	1791	N/A	INTRINSIC
low complexity region	1835	1851	N/A	INTRINSIC
internal_repeat_5	1857	1900	8.04e-5	PROSPERO
internal_repeat_6	1887	1911	8.04e-5	PROSPERO
internal_repeat_3	1893	1955	1.07e-5	PROSPERO
internal_repeat_4	1949	1969	4.1e-5	PROSPERO
internal_repeat_1	1967	1993	1.42e-6	PROSPERO
low complexity region	1994	2007	N/A	INTRINSIC
low complexity region	2016	2055	N/A	INTRINSIC
low complexity region	2063	2088	N/A	INTRINSIC
internal_repeat_4	2091	2110	4.1e-5	PROSPERO
internal_repeat_6	2108	2132	8.04e-5	PROSPERO
low complexity region	2133	2152	N/A	INTRINSIC
internal_repeat_2	2158	2209	2.78e-6	PROSPERO
internal_repeat_1	2228	2253	1.42e-6	PROSPERO
internal_repeat_2	2230	2286	2.78e-6	PROSPERO
low complexity region	2313	2325	N/A	INTRINSIC
low complexity region	2337	2351	N/A	INTRINSIC
low complexity region	2366	2388	N/A	INTRINSIC
low complexity region	2420	2431	N/A	INTRINSIC

Predicted Effect

probably benign
Transcript: ENSMUST00000161320

SMART Domains

Protein: ENSMUSP00000124801
Gene: ENSMUSG00000006014

Domain	Start	End	E-Value	Type
signal peptide	1	24	N/A	INTRINSIC
SO	25	67	3.83e-15	SMART
low complexity region	69	83	N/A	INTRINSIC
low complexity region	100	110	N/A	INTRINSIC
low complexity region	148	179	N/A	INTRINSIC

Predicted Effect

unknown
Transcript: ENSMUST00000161611
AA Change: C1018Y

SMART Domains

Protein: ENSMUSP00000125677
Gene: ENSMUSG00000006014
AA Change: C1018Y

Domain	Start	End	E-Value	Type
signal peptide	1	24	N/A	INTRINSIC
SO	26	68	9.21e-18	SMART
SO	69	108	2.77e-12	SMART
low complexity region	110	122	N/A	INTRINSIC
low complexity region	127	184	N/A	INTRINSIC
low complexity region	191	212	N/A	INTRINSIC
low complexity region	229	239	N/A	INTRINSIC
internal_repeat_3	244	271	7.42e-5	PROSPERO
low complexity region	277	309	N/A	INTRINSIC
Pfam:Cornifin	578	691	2.2e-9	PFAM
internal_repeat_3	796	825	7.42e-5	PROSPERO
internal_repeat_2	797	823	1.24e-5	PROSPERO
low complexity region	826	879	N/A	INTRINSIC
internal_repeat_2	889	915	1.24e-5	PROSPERO
HX	967	1009	1.67e-7	SMART
HX	1011	1054	3.76e-10	SMART
Blast:HX	1167	1220	6e-27	BLAST

Predicted Effect

probably damaging
Transcript: ENSMUST00000162367
AA Change: C220Y

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)

SMART Domains

Protein: ENSMUSP00000125551
Gene: ENSMUSG00000006014
AA Change: C220Y

Domain	Start	End	E-Value	Type
signal peptide	1	24	N/A	INTRINSIC
SO	26	68	9.21e-18	SMART
SO	69	108	4.21e-12	SMART
low complexity region	110	124	N/A	INTRINSIC
HX	169	211	1.67e-7	SMART
HX	213	256	3.76e-10	SMART
Blast:HX	369	422	2e-27	BLAST

Predicted Effect

unknown
Transcript: ENSMUST00000164600
AA Change: C1018Y

SMART Domains

Protein: ENSMUSP00000128943
Gene: ENSMUSG00000006014
AA Change: C1018Y

Domain	Start	End	E-Value	Type
signal peptide	1	24	N/A	INTRINSIC
SO	26	68	9.21e-18	SMART
SO	69	108	2.77e-12	SMART
low complexity region	110	122	N/A	INTRINSIC
low complexity region	127	184	N/A	INTRINSIC
low complexity region	191	212	N/A	INTRINSIC
low complexity region	229	239	N/A	INTRINSIC
internal_repeat_3	244	271	7.42e-5	PROSPERO
low complexity region	277	309	N/A	INTRINSIC
Pfam:Cornifin	404	497	2.4e-14	PFAM
Pfam:Cornifin	468	584	3.2e-15	PFAM
Pfam:Cornifin	550	648	3.3e-16	PFAM
Pfam:Cornifin	625	722	2.1e-15	PFAM
Pfam:Cornifin	647	743	7.8e-16	PFAM
Pfam:Cornifin	706	822	1.4e-12	PFAM
low complexity region	826	879	N/A	INTRINSIC
internal_repeat_2	889	915	1.24e-5	PROSPERO
HX	967	1009	1.67e-7	SMART
HX	1011	1054	3.76e-10	SMART
Blast:HX	1167	1220	6e-27	BLAST

Coding Region Coverage

1x: 100.0%
3x: 100.0%
10x: 99.7%
20x: 99.0%

Validation Efficiency

MGI Phenotype

PHENOTYPE: Aging homozygous null mice develop a hopping gait, irregular endochondral growth plates, altered cartilage surface, camptodactyly, progressive synovial hyperplasia and, ultimately, joint failure. [provided by MGI curators]

Allele List at MGI

Other mutations in this stock

Total: 98 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
1700020D05Rik	T	A	19: 5,503,595	T53S	probably benign	Het
4932438A13Rik	T	G	3: 36,940,798	Y990D	probably damaging	Het
Abca13	A	T	11: 9,335,215	H3283L	probably damaging	Het
Abcb1a	T	G	5: 8,694,072	S233A	probably benign	Het
Actr10	T	C	12: 70,953,031		probably null	Het
Acvr2b	C	T	9: 119,432,553	A380V	probably damaging	Het
Adcy5	A	G	16: 35,299,625	E1168G	probably damaging	Het
Akap12	C	T	10: 4,353,226	T117I	probably damaging	Het
Alox5	T	A	6: 116,413,468	Y516F	probably benign	Het
Amigo2	T	C	15: 97,245,860	N227S	probably damaging	Het
Anpep	C	A	7: 79,836,313	V554L	probably benign	Het
Ap5b1	T	A	19: 5,570,187	V545E	possibly damaging	Het
Bbs9	C	T	9: 22,579,553	L326F	probably damaging	Het
Cadps	C	T	14: 12,603,738	G361R	probably damaging	Het
Ccdc57	C	A	11: 120,921,731	E66*	probably null	Het
Ccr6	G	A	17: 8,256,187	V75I	probably benign	Het
Cdk6	T	C	5: 3,520,709	F300S	probably damaging	Het
Cenpb	A	C	2: 131,178,879	V333G	probably damaging	Het
Clca4a	T	C	3: 144,961,909	I434V	probably benign	Het
Coro2b	T	A	9: 62,421,385	D447V	possibly damaging	Het
Cpeb3	T	C	19: 37,174,719	S86G	probably benign	Het
Cry2	C	A	2: 92,413,093	A468S	probably damaging	Het
Csf2rb2	T	C	15: 78,297,072	Y40C	probably damaging	Het
Ddx49	T	A	8: 70,301,076	T48S	probably damaging	Het
Dip2c	A	G	13: 9,604,536	T727A	probably benign	Het
Ebf4	A	T	2: 130,309,731	I183F	probably benign	Het
Elavl3	G	T	9: 22,018,729	P293Q	possibly damaging	Het
Esyt1	A	T	10: 128,516,236	L768Q	probably damaging	Het
Fam45a	T	A	19: 60,832,596	M272K	probably damaging	Het
Fat2	G	A	11: 55,283,434	P2151L	probably damaging	Het
Fgfbp3	G	T	19: 36,919,206	A4E	possibly damaging	Het
Flg	T	A	3: 93,293,028	V277D	unknown	Het
Fndc3b	T	C	3: 27,470,234	D459G	possibly damaging	Het
Fras1	A	G	5: 96,571,041	Q438R	probably benign	Het
Glipr1l2	A	T	10: 112,092,425		probably null	Het
Gm7347	T	A	5: 26,057,384		probably null	Het
Grm6	G	A	11: 50,862,977	V703I	possibly damaging	Het
Gtf2h1	T	A	7: 46,819,126	V496E	probably benign	Het
Ifna12	A	G	4: 88,603,151	L53P	probably damaging	Het
Invs	T	A	4: 48,407,674	S550T	probably benign	Het
Irak2	G	T	6: 113,686,849	C453F	probably damaging	Het
Itih2	G	T	2: 10,105,763	Q506K	probably benign	Het
Kidins220	T	C	12: 25,057,663	I1614T	probably benign	Het
Krtap5-3	T	A	7: 142,202,255	C276*	probably null	Het
Lama3	T	A	18: 12,552,813	M1128K	possibly damaging	Het
Lhfpl4	C	T	6: 113,194,145	A27T	possibly damaging	Het
Lyplal1	A	G	1: 186,100,327	V77A	probably damaging	Het
Malrd1	A	T	2: 16,142,303	E1985D	unknown	Het
Mlx	A	C	11: 101,088,976	Q161P	probably benign	Het
Mroh2b	T	A	15: 4,948,003	M1279K	probably benign	Het
Myh7b	A	G	2: 155,622,199	E540G	probably damaging	Het
Neb	T	A	2: 52,304,055	D653V	probably damaging	Het
Nek10	T	A	14: 14,828,517	L113Q	probably damaging	Het
Nlrp4c	T	A	7: 6,065,709	L203*	probably null	Het
Ntpcr	T	A	8: 125,730,055	C5S	unknown	Het
Nwd1	A	G	8: 72,695,329	D1001G	probably damaging	Het
Olfr1184	T	A	2: 88,487,148	C139S	probably damaging	Het
Olfr294	C	T	7: 86,616,267	C126Y	probably benign	Het
Olfr536	T	A	7: 140,504,316	T48S	probably benign	Het
Olfr784	A	C	10: 129,388,167	D178A	probably damaging	Het
Osbpl3	T	A	6: 50,320,135	S564C	probably damaging	Het
Pax6	C	A	2: 105,692,259	P264T	probably damaging	Het
Plppr4	T	C	3: 117,323,183	R342G	probably damaging	Het
Prune2	T	A	19: 17,121,213	D1360E	probably benign	Het
Ranbp2	T	A	10: 58,463,950	S469T	probably damaging	Het
Rbfox1	A	T	16: 7,369,834	K43N	probably benign	Het
Rgs22	T	C	15: 36,122,313	D25G	probably damaging	Het
Rnf113a2	G	A	12: 84,417,771	G146S	probably damaging	Het
Sbpl	T	A	17: 23,954,634	K55*	probably null	Het
Scel	G	A	14: 103,543,832	W138*	probably null	Het
Scgb2b19	T	C	7: 33,280,286	I12V	probably null	Het
Scn9a	T	A	2: 66,549,015	M358L	probably damaging	Het
Sdk2	C	A	11: 113,842,690	E924*	probably null	Het
Sipa1l3	T	C	7: 29,348,587	Q1292R	possibly damaging	Het
Skint4	G	A	4: 112,118,101	G86D	probably damaging	Het
Slc28a3	C	T	13: 58,588,214	V57I	probably benign	Het
Slc9a4	C	T	1: 40,580,639	P42S	probably benign	Het
Slc9a4	T	C	1: 40,623,399	S609P	probably damaging	Het
Slitrk1	T	A	14: 108,912,629	T217S	probably benign	Het
Spindoc	C	T	19: 7,358,442	R327H	probably benign	Het
Stk36	T	C	1: 74,622,223	S470P	probably benign	Het
Sypl	C	T	12: 32,974,255	P196L	probably benign	Het
Tekt4	T	A	17: 25,474,744	I285N	probably damaging	Het
Tgm5	T	A	2: 121,046,498	I686F	possibly damaging	Het
Thsd4	C	A	9: 59,976,304	R933L	probably damaging	Het
Treml1	T	A	17: 48,366,672	I237N	probably damaging	Het
Txndc16	T	C	14: 45,205,382	I119V	probably benign	Het
Ubr3	C	A	2: 69,897,822	N176K	probably damaging	Het
Vit	A	G	17: 78,624,997	Y511C	probably damaging	Het
Vwa5b2	G	A	16: 20,604,234	G994D	probably benign	Het
Wdr63	A	T	3: 146,055,704	S632R	possibly damaging	Het
Wnk1	T	C	6: 119,948,307	T1648A	unknown	Het
Ythdf1	G	A	2: 180,911,522	T300I	probably damaging	Het
Zan	A	G	5: 137,454,200		probably null	Het
Zbbx	A	G	3: 75,112,094	L103P	probably benign	Het
Zfp105	A	G	9: 122,929,804	D180G	probably damaging	Het
Zfp114	T	A	7: 24,180,658	L144Q	possibly damaging	Het
Zfp128	T	C	7: 12,890,472	C256R	probably damaging	Het

Other mutations in Prg4

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00493:Prg4	APN	1	150451920	missense	probably damaging	0.99
IGL02033:Prg4	APN	1	150455868	intron	probably benign
IGL02154:Prg4	APN	1	150454862	intron	probably benign
IGL03111:Prg4	APN	1	150451902	missense	probably benign	0.06
IGL03177:Prg4	APN	1	150455603	intron	probably benign
IGL03260:Prg4	APN	1	150455627	intron	probably benign
IGL03281:Prg4	APN	1	150450088	splice site	probably benign
R0046:Prg4	UTSW	1	150456086	missense	possibly damaging	0.53
R0046:Prg4	UTSW	1	150456086	missense	possibly damaging	0.53
R0196:Prg4	UTSW	1	150454492	intron	probably benign
R0233:Prg4	UTSW	1	150453547	splice site	probably benign
R0255:Prg4	UTSW	1	150455807	intron	probably benign
R0616:Prg4	UTSW	1	150460711	missense	probably damaging	1.00
R1016:Prg4	UTSW	1	150454691	intron	probably benign
R1826:Prg4	UTSW	1	150452009	missense	probably benign	0.09
R1862:Prg4	UTSW	1	150460669	missense	probably damaging	0.99
R1863:Prg4	UTSW	1	150460669	missense	probably damaging	0.99
R1922:Prg4	UTSW	1	150449999	nonsense	probably null
R1940:Prg4	UTSW	1	150456023	missense	possibly damaging	0.53
R3765:Prg4	UTSW	1	150451371	missense	probably damaging	0.97
R3855:Prg4	UTSW	1	150452000	missense	probably damaging	1.00
R3894:Prg4	UTSW	1	150454759	intron	probably benign
R3895:Prg4	UTSW	1	150454759	intron	probably benign
R3912:Prg4	UTSW	1	150451868	missense	probably damaging	1.00
R3935:Prg4	UTSW	1	150458157	missense	possibly damaging	0.68
R4050:Prg4	UTSW	1	150454759	intron	probably benign
R4475:Prg4	UTSW	1	150454859	intron	probably benign
R4794:Prg4	UTSW	1	150454546	intron	probably benign
R4910:Prg4	UTSW	1	150455823	intron	probably benign
R4911:Prg4	UTSW	1	150455823	intron	probably benign
R4993:Prg4	UTSW	1	150460681	missense	probably damaging	1.00
R5378:Prg4	UTSW	1	150455226	intron	probably benign
R5381:Prg4	UTSW	1	150454453	intron	probably benign
R5452:Prg4	UTSW	1	150455768	intron	probably benign
R5870:Prg4	UTSW	1	150455549	nonsense	probably null
R5888:Prg4	UTSW	1	150452350	missense	probably damaging	1.00
R5929:Prg4	UTSW	1	150454129	missense	probably benign	0.01
R6058:Prg4	UTSW	1	150451446	missense	probably damaging	0.99
R6059:Prg4	UTSW	1	150449997	missense	possibly damaging	0.67
R6232:Prg4	UTSW	1	150455816	intron	probably benign
R6272:Prg4	UTSW	1	150454766	intron	probably benign
R6459:Prg4	UTSW	1	150454301	intron	probably benign
R6659:Prg4	UTSW	1	150460681	missense	probably damaging	1.00
R6663:Prg4	UTSW	1	150455101	intron	probably benign
R6882:Prg4	UTSW	1	150453495	missense	probably damaging	1.00
R6970:Prg4	UTSW	1	150455906	intron	probably benign
R7078:Prg4	UTSW	1	150458263	missense	possibly damaging	0.91
R7264:Prg4	UTSW	1	150454067	missense	not run
R7487:Prg4	UTSW	1	150455905	missense	unknown
R7531:Prg4	UTSW	1	150455035	missense	unknown
R7651:Prg4	UTSW	1	150454945	missense	unknown
R7701:Prg4	UTSW	1	150457542	missense	possibly damaging	0.53
X0024:Prg4	UTSW	1	150454492	intron	probably benign

Predicted Primers

PCR Primer
(F):5'- TCATCCTCAGAATTAAGTGTAGGGAG -3'
(R):5'- AGGTCCAGGCACTTCTTAACAC -3'

Sequencing Primer
(F):5'- GTAGGGAGAATACAATAAATGGGTTG -3'
(R):5'- GTCCAGGCACTTCTTAACACATTGAC -3'

Posted On

2019-05-15