Mutagenetix > Incidental Mutation

Incidental Mutation 'R7106:Zfp27'

551177

Institutional Source

Beutler Lab

Gene Symbol

Zfp27

Ensembl Gene

ENSMUSG00000062040

Gene Name

zinc finger protein 27

Synonyms

Zfp-27, mkr-4

MMRRC Submission

045198-MU

Accession Numbers

Essential gene?

Possibly essential (E-score: 0.611) question?

Stock #

R7106 (G1)

Quality Score

217.468

Status

Validated

Chromosome

Chromosomal Location

29592758-29605997 bp(-) (GRCm39)

Type of Mutation

small deletion (4 aa in frame mutation)

DNA Base Change (assembly)

AATCCGCTTGTGCA to AA at 29594446 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Ref Sequence

ENSEMBL: ENSMUSP00000127677 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000053521] [ENSMUST00000159920] [ENSMUST00000161904] [ENSMUST00000162592] [ENSMUST00000172448]

AlphaFold

P10077

Predicted Effect

probably benign
Transcript: ENSMUST00000053521

SMART Domains

Protein: ENSMUSP00000054012
Gene: ENSMUSG00000062040

Domain	Start	End	E-Value	Type
KRAB	1	59	2.94e-12	SMART
internal_repeat_2	108	195	4.1e-6	PROSPERO
ZnF_C2H2	205	227	3.11e-2	SMART
ZnF_C2H2	233	255	8.47e-4	SMART
ZnF_C2H2	261	283	2.09e-3	SMART
ZnF_C2H2	289	311	2.91e-2	SMART
ZnF_C2H2	317	339	4.17e-3	SMART
ZnF_C2H2	345	367	4.79e-3	SMART
ZnF_C2H2	401	423	7.15e-2	SMART
ZnF_C2H2	429	451	3.69e-4	SMART
ZnF_C2H2	457	479	1.08e-1	SMART
ZnF_C2H2	485	507	2.57e-3	SMART
ZnF_C2H2	513	535	1.5e-4	SMART
ZnF_C2H2	541	563	1.38e-3	SMART
ZnF_C2H2	569	591	1.03e-2	SMART
ZnF_C2H2	597	619	3.34e-2	SMART
ZnF_C2H2	625	647	8.34e-3	SMART
ZnF_C2H2	653	675	2.36e-2	SMART
ZnF_C2H2	681	703	6.88e-4	SMART
ZnF_C2H2	709	731	2.27e-4	SMART
ZnF_C2H2	737	759	6.88e-4	SMART
ZnF_C2H2	765	787	1.72e-4	SMART
ZnF_C2H2	793	815	3.83e-2	SMART

Predicted Effect

probably benign
Transcript: ENSMUST00000159920

SMART Domains

Protein: ENSMUSP00000125232
Gene: ENSMUSG00000062040

Domain	Start	End	E-Value	Type
KRAB	1	59	2.94e-12	SMART
internal_repeat_2	108	195	4.1e-6	PROSPERO
ZnF_C2H2	205	227	3.11e-2	SMART
ZnF_C2H2	233	255	8.47e-4	SMART
ZnF_C2H2	261	283	2.09e-3	SMART
ZnF_C2H2	289	311	2.91e-2	SMART
ZnF_C2H2	317	339	4.17e-3	SMART
ZnF_C2H2	345	367	4.79e-3	SMART
ZnF_C2H2	401	423	7.15e-2	SMART
ZnF_C2H2	429	451	3.69e-4	SMART
ZnF_C2H2	457	479	1.08e-1	SMART
ZnF_C2H2	485	507	2.57e-3	SMART
ZnF_C2H2	513	535	1.5e-4	SMART
ZnF_C2H2	541	563	1.38e-3	SMART
ZnF_C2H2	569	591	1.03e-2	SMART
ZnF_C2H2	597	619	3.34e-2	SMART
ZnF_C2H2	625	647	8.34e-3	SMART
ZnF_C2H2	653	675	2.36e-2	SMART
ZnF_C2H2	681	703	6.88e-4	SMART
ZnF_C2H2	709	731	2.27e-4	SMART
ZnF_C2H2	737	759	6.88e-4	SMART
ZnF_C2H2	765	787	1.72e-4	SMART
ZnF_C2H2	793	815	3.83e-2	SMART

Predicted Effect

probably benign
Transcript: ENSMUST00000161904

SMART Domains

Protein: ENSMUSP00000124684
Gene: ENSMUSG00000062040

Domain	Start	End	E-Value	Type
KRAB	1	59	2.94e-12	SMART
internal_repeat_2	108	195	4.1e-6	PROSPERO
ZnF_C2H2	205	227	3.11e-2	SMART
ZnF_C2H2	233	255	8.47e-4	SMART
ZnF_C2H2	261	283	2.09e-3	SMART
ZnF_C2H2	289	311	2.91e-2	SMART
ZnF_C2H2	317	339	4.17e-3	SMART
ZnF_C2H2	345	367	4.79e-3	SMART
ZnF_C2H2	401	423	7.15e-2	SMART
ZnF_C2H2	429	451	3.69e-4	SMART
ZnF_C2H2	457	479	1.08e-1	SMART
ZnF_C2H2	485	507	2.57e-3	SMART
ZnF_C2H2	513	535	1.5e-4	SMART
ZnF_C2H2	541	563	1.38e-3	SMART
ZnF_C2H2	569	591	1.03e-2	SMART
ZnF_C2H2	597	619	3.34e-2	SMART
ZnF_C2H2	625	647	8.34e-3	SMART
ZnF_C2H2	653	675	2.36e-2	SMART
ZnF_C2H2	681	703	6.88e-4	SMART
ZnF_C2H2	709	731	2.27e-4	SMART
ZnF_C2H2	737	759	6.88e-4	SMART
ZnF_C2H2	765	787	1.72e-4	SMART
ZnF_C2H2	793	815	3.83e-2	SMART

Predicted Effect

probably benign
Transcript: ENSMUST00000162592

SMART Domains

Protein: ENSMUSP00000123953
Gene: ENSMUSG00000062040

Domain	Start	End	E-Value	Type
KRAB	1	59	2.94e-12	SMART
internal_repeat_2	108	195	4.1e-6	PROSPERO
ZnF_C2H2	205	227	3.11e-2	SMART
ZnF_C2H2	233	255	8.47e-4	SMART
ZnF_C2H2	261	283	2.09e-3	SMART
ZnF_C2H2	289	311	2.91e-2	SMART
ZnF_C2H2	317	339	4.17e-3	SMART
ZnF_C2H2	345	367	4.79e-3	SMART
ZnF_C2H2	401	423	7.15e-2	SMART
ZnF_C2H2	429	451	3.69e-4	SMART
ZnF_C2H2	457	479	1.08e-1	SMART
ZnF_C2H2	485	507	2.57e-3	SMART
ZnF_C2H2	513	535	1.5e-4	SMART
ZnF_C2H2	541	563	1.38e-3	SMART
ZnF_C2H2	569	591	1.03e-2	SMART
ZnF_C2H2	597	619	3.34e-2	SMART
ZnF_C2H2	625	647	8.34e-3	SMART
ZnF_C2H2	653	675	2.36e-2	SMART
ZnF_C2H2	681	703	6.88e-4	SMART
ZnF_C2H2	709	731	2.27e-4	SMART
ZnF_C2H2	737	759	6.88e-4	SMART
ZnF_C2H2	765	787	1.72e-4	SMART
ZnF_C2H2	793	815	3.83e-2	SMART

Predicted Effect

probably benign
Transcript: ENSMUST00000172448

SMART Domains

Protein: ENSMUSP00000127677
Gene: ENSMUSG00000062040

Domain	Start	End	E-Value	Type
KRAB	1	59	2.94e-12	SMART
internal_repeat_2	108	195	4.1e-6	PROSPERO
ZnF_C2H2	205	227	3.11e-2	SMART
ZnF_C2H2	233	255	8.47e-4	SMART
ZnF_C2H2	261	283	2.09e-3	SMART
ZnF_C2H2	289	311	2.91e-2	SMART
ZnF_C2H2	317	339	4.17e-3	SMART
ZnF_C2H2	345	367	4.79e-3	SMART
ZnF_C2H2	401	423	7.15e-2	SMART
ZnF_C2H2	429	451	3.69e-4	SMART
ZnF_C2H2	457	479	1.08e-1	SMART
ZnF_C2H2	485	507	2.57e-3	SMART
ZnF_C2H2	513	535	1.5e-4	SMART
ZnF_C2H2	541	563	1.38e-3	SMART
ZnF_C2H2	569	591	1.03e-2	SMART
ZnF_C2H2	597	619	3.34e-2	SMART
ZnF_C2H2	625	647	8.34e-3	SMART
ZnF_C2H2	653	675	2.36e-2	SMART
ZnF_C2H2	681	703	6.88e-4	SMART
ZnF_C2H2	709	731	2.27e-4	SMART
ZnF_C2H2	737	759	6.88e-4	SMART
ZnF_C2H2	765	787	1.72e-4	SMART
ZnF_C2H2	793	815	3.83e-2	SMART

Coding Region Coverage

1x: 100.0%
3x: 99.9%
10x: 99.6%
20x: 98.8%

Validation Efficiency

100% (62/62)

Allele List at MGI

Other mutations in this stock

Total: 63 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Aldob	T	C	4: 49,541,258 (GRCm39)	Y138C	probably damaging	Het
Ano7	A	C	1: 93,302,705 (GRCm39)		probably null	Het
Arvcf	T	A	16: 18,217,799 (GRCm39)	V497E	probably damaging	Het
Atad2	A	G	15: 57,980,162 (GRCm39)		probably null	Het
Brinp1	C	T	4: 68,747,615 (GRCm39)	A163T	probably benign	Het
Catsperd	C	A	17: 56,965,070 (GRCm39)		probably null	Het
Ccm2l	A	G	2: 152,912,571 (GRCm39)	H70R	possibly damaging	Het
Cgnl1	G	A	9: 71,633,015 (GRCm39)	T112I	probably benign	Het
Chid1	T	C	7: 141,102,573 (GRCm39)	H220R	probably benign	Het
Clca3a1	A	G	3: 144,733,190 (GRCm39)	V106A	probably damaging	Het
Cldn23	A	C	8: 36,293,069 (GRCm39)	S140A	probably benign	Het
Dennd2c	T	C	3: 103,038,893 (GRCm39)	S14P	possibly damaging	Het
Det1	T	C	7: 78,493,212 (GRCm39)	D264G	probably damaging	Het
Dmwd	T	C	7: 18,814,454 (GRCm39)	V368A	probably damaging	Het
Dnah11	T	C	12: 117,924,884 (GRCm39)	K3362E	probably benign	Het
Dnah8	T	C	17: 30,960,152 (GRCm39)	F2208L	probably damaging	Het
Dnaja3	T	C	16: 4,523,798 (GRCm39)	I477T	probably benign	Het
Dnmt3a	A	G	12: 3,947,591 (GRCm39)	K482E	probably damaging	Het
Dock7	A	G	4: 98,855,563 (GRCm39)	I1493T	unknown	Het
Ehbp1l1	A	G	19: 5,768,765 (GRCm39)	V846A	probably benign	Het
Fbxl4	T	A	4: 22,427,140 (GRCm39)		probably null	Het
Fgd2	T	A	17: 29,595,944 (GRCm39)	L534*	probably null	Het
Fgfr3	T	C	5: 33,888,758 (GRCm39)	V349A	probably damaging	Het
Gal3st1	A	T	11: 3,948,509 (GRCm39)	I239F	probably damaging	Het
Gpatch3	C	A	4: 133,305,514 (GRCm39)	H250N	probably benign	Het
Guca1b	A	T	17: 47,702,236 (GRCm39)	K230*	probably null	Het
Itga3	G	T	11: 94,946,699 (GRCm39)	L737I	probably benign	Het
Kmt5c	A	G	7: 4,745,705 (GRCm39)	K122E	probably damaging	Het
Kpna4	G	A	3: 68,986,797 (GRCm39)	Q531*	probably null	Het
Lctl	A	G	9: 64,040,119 (GRCm39)	E410G	probably benign	Het
Lhx1	T	C	11: 84,412,903 (GRCm39)	N128D	probably benign	Het
Lifr	A	G	15: 7,202,405 (GRCm39)	N349D	probably benign	Het
Map2	G	A	1: 66,449,903 (GRCm39)	A209T	possibly damaging	Het
Mif4gd	G	A	11: 115,502,737 (GRCm39)	A25V	probably damaging	Het
Mug2	T	C	6: 122,059,680 (GRCm39)	S1353P	probably damaging	Het
Myh9	A	G	15: 77,659,321 (GRCm39)	C931R	probably benign	Het
Nelfe	T	G	17: 35,071,395 (GRCm39)		probably null	Het
Nrf1	A	G	6: 30,102,183 (GRCm39)	S161G	probably benign	Het
Pik3c2a	G	A	7: 116,017,368 (GRCm39)	Q130*	probably null	Het
Prex2	T	A	1: 11,207,017 (GRCm39)	M525K	probably benign	Het
Psme2	A	G	14: 55,825,694 (GRCm39)	S165P	probably benign	Het
Rapgef2	A	G	3: 78,973,915 (GRCm39)	F1477S	probably benign	Het
Rasef	A	T	4: 73,645,864 (GRCm39)	C502S	probably damaging	Het
Rpap2	T	A	5: 107,780,988 (GRCm39)	L565*	probably null	Het
Sav1	A	G	12: 70,031,390 (GRCm39)	I44T	probably damaging	Het
Selp	T	A	1: 163,953,991 (GRCm39)	I97N	probably benign	Het
Serpina1d	A	G	12: 103,731,980 (GRCm39)	F293S	probably benign	Het
Sin3b	T	C	8: 73,450,765 (GRCm39)	F65L	possibly damaging	Het
Sorcs3	G	C	19: 48,694,402 (GRCm39)	G559R	probably damaging	Het
Susd2	A	T	10: 75,473,887 (GRCm39)	D689E	probably damaging	Het
Tas1r2	A	T	4: 139,389,360 (GRCm39)	M448L	probably benign	Het
Tbc1d30	A	T	10: 121,137,897 (GRCm39)	I181N	possibly damaging	Het
Tcea3	A	G	4: 135,998,679 (GRCm39)	T318A	probably damaging	Het
Tomm70a	T	C	16: 56,961,121 (GRCm39)	V358A	probably damaging	Het
Tpp1	A	C	7: 105,399,118 (GRCm39)	S153A	possibly damaging	Het
Trip13	C	T	13: 74,062,651 (GRCm39)	V387I	probably benign	Het
Ttn	T	C	2: 76,627,832 (GRCm39)	I14704V	probably benign	Het
Uimc1	C	A	13: 55,198,628 (GRCm39)	C516F	possibly damaging	Het
Vit	A	G	17: 78,894,228 (GRCm39)	N210S	probably benign	Het
Vmn1r22	G	T	6: 57,877,296 (GRCm39)	T227K	probably damaging	Het
Vmn2r5	A	G	3: 64,399,104 (GRCm39)	I625T	probably benign	Het
Zfp384	T	C	6: 125,001,222 (GRCm39)	L98P	probably benign	Het
Zfp809	G	T	9: 22,147,520 (GRCm39)	K51N	probably benign	Het

Other mutations in Zfp27

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00972:Zfp27	APN	7	29,594,383 (GRCm39)	missense	probably damaging	0.97
IGL02490:Zfp27	APN	7	29,594,360 (GRCm39)	missense	possibly damaging	0.73
IGL02899:Zfp27	APN	7	29,595,680 (GRCm39)	missense	possibly damaging	0.91
R0179:Zfp27	UTSW	7	29,595,850 (GRCm39)	missense	possibly damaging	0.51
R0234:Zfp27	UTSW	7	29,593,532 (GRCm39)	missense	possibly damaging	0.73
R0234:Zfp27	UTSW	7	29,593,532 (GRCm39)	missense	possibly damaging	0.73
R0511:Zfp27	UTSW	7	29,593,947 (GRCm39)	missense	probably damaging	0.97
R1185:Zfp27	UTSW	7	29,595,254 (GRCm39)	missense	possibly damaging	0.92
R1185:Zfp27	UTSW	7	29,595,254 (GRCm39)	missense	possibly damaging	0.92
R1185:Zfp27	UTSW	7	29,595,254 (GRCm39)	missense	possibly damaging	0.92
R1294:Zfp27	UTSW	7	29,595,737 (GRCm39)	missense	possibly damaging	0.53
R1581:Zfp27	UTSW	7	29,595,549 (GRCm39)	missense	possibly damaging	0.53
R1763:Zfp27	UTSW	7	29,594,801 (GRCm39)	missense	possibly damaging	0.96
R2083:Zfp27	UTSW	7	29,594,208 (GRCm39)	missense	probably benign	0.06
R2217:Zfp27	UTSW	7	29,595,536 (GRCm39)	missense	possibly damaging	0.96
R2696:Zfp27	UTSW	7	29,595,792 (GRCm39)	missense	possibly damaging	0.85
R4084:Zfp27	UTSW	7	29,594,792 (GRCm39)	missense	possibly damaging	0.91
R4864:Zfp27	UTSW	7	29,594,261 (GRCm39)	missense	probably damaging	0.99
R6057:Zfp27	UTSW	7	29,594,444 (GRCm39)	missense	possibly damaging	0.83
R6063:Zfp27	UTSW	7	29,593,727 (GRCm39)	missense	probably damaging	0.97
R6553:Zfp27	UTSW	7	29,595,818 (GRCm39)	missense	possibly damaging	0.86
R6585:Zfp27	UTSW	7	29,595,818 (GRCm39)	missense	possibly damaging	0.86
R6800:Zfp27	UTSW	7	29,593,860 (GRCm39)	missense	probably benign	0.19
R7051:Zfp27	UTSW	7	29,594,446 (GRCm39)	small deletion	probably benign
R7052:Zfp27	UTSW	7	29,594,446 (GRCm39)	small deletion	probably benign
R7066:Zfp27	UTSW	7	29,594,446 (GRCm39)	small deletion	probably benign
R7432:Zfp27	UTSW	7	29,594,784 (GRCm39)	missense	probably benign	0.33
R7473:Zfp27	UTSW	7	29,595,324 (GRCm39)	missense	possibly damaging	0.71
R7670:Zfp27	UTSW	7	29,594,221 (GRCm39)	missense	possibly damaging	0.94
R7739:Zfp27	UTSW	7	29,593,699 (GRCm39)	missense	possibly damaging	0.93
R7817:Zfp27	UTSW	7	29,595,815 (GRCm39)	missense	possibly damaging	0.96
R8750:Zfp27	UTSW	7	29,594,804 (GRCm39)	missense	possibly damaging	0.53
R8819:Zfp27	UTSW	7	29,594,013 (GRCm39)	missense	probably benign	0.15
R8820:Zfp27	UTSW	7	29,594,013 (GRCm39)	missense	probably benign	0.15
R9189:Zfp27	UTSW	7	29,595,359 (GRCm39)	missense	possibly damaging	0.76
R9511:Zfp27	UTSW	7	29,593,641 (GRCm39)	missense	possibly damaging	0.71
R9705:Zfp27	UTSW	7	29,595,342 (GRCm39)	missense	possibly damaging	0.93
Z1177:Zfp27	UTSW	7	29,595,657 (GRCm39)	missense	possibly damaging	0.72
Z1177:Zfp27	UTSW	7	29,594,586 (GRCm39)	missense	probably benign	0.33

Predicted Primers

PCR Primer
(F):5'- TCTGGGTAAAGGCTTTCCCG -3'
(R):5'- ATCTCTCGACCGTGCGAAAC -3'

Sequencing Primer
(F):5'- GTGCTACACCCATATGGCTTC -3'
(R):5'- CAGGTCGGAGCTAATCATTCATCAG -3'

Posted On

2019-05-15