Incidental Mutation 'R7109:Olfr828'
ID551400
Institutional Source Beutler Lab
Gene Symbol Olfr828
Ensembl Gene ENSMUSG00000078116
Gene Nameolfactory receptor 828
SynonymsMOR149-1, GA_x6K02T2PVTD-12559294-12558356
MMRRC Submission
Accession Numbers
Is this an essential gene? Probably non essential (E-score: 0.124) question?
Stock #R7109 (G1)
Quality Score225.009
Status Not validated
Chromosome9
Chromosomal Location18814728-18819526 bp(-) (GRCm38)
Type of Mutationmissense
DNA Base Change (assembly) A to T at 18815608 bp
ZygosityHeterozygous
Amino Acid Change Serine to Threonine at position 229 (S229T)
Ref Sequence ENSEMBL: ENSMUSP00000148853 (fasta)
Gene Model predicted gene model for transcript(s): [ENSMUST00000104914] [ENSMUST00000215380]
Predicted Effect probably benign
Transcript: ENSMUST00000104914
AA Change: S229T

PolyPhen 2 Score 0.006 (Sensitivity: 0.97; Specificity: 0.75)
SMART Domains Protein: ENSMUSP00000100514
Gene: ENSMUSG00000078116
AA Change: S229T

DomainStartEndE-ValueType
Pfam:7tm_4 31 310 1e-48 PFAM
Pfam:7TM_GPCR_Srsx 35 305 1.4e-5 PFAM
Pfam:7tm_1 41 290 2.1e-21 PFAM
Predicted Effect probably benign
Transcript: ENSMUST00000215380
AA Change: S229T

PolyPhen 2 Score 0.006 (Sensitivity: 0.97; Specificity: 0.75)
Coding Region Coverage
  • 1x: 100.0%
  • 3x: 100.0%
  • 10x: 99.7%
  • 20x: 99.1%
Validation Efficiency
MGI Phenotype FUNCTION: Olfactory receptors interact with odorant molecules in the nose, to initiate a neuronal response that triggers the perception of a smell. The olfactory receptor proteins are members of a large family of G-protein-coupled receptors (GPCR) arising from single coding-exon genes. Olfactory receptors share a 7-transmembrane domain structure with many neurotransmitter and hormone receptors and are responsible for the recognition and G protein-mediated transduction of odorant signals. The olfactory receptor gene family is the largest in the genome. The nomenclature assigned to the olfactory receptor genes and proteins for this organism is independent of other organisms. [provided by RefSeq, Jul 2008]
Allele List at MGI
Other mutations in this stock
Total: 52 list
GeneRefVarChr/LocMutationPredicted EffectZygosity
4921509C19Rik T G 2: 151,473,753 K2Q probably damaging Het
Abtb2 T C 2: 103,715,515 Y903H probably benign Het
Adamtsl3 C T 7: 82,611,861 P29S Het
Alcam T A 16: 52,276,829 T355S probably damaging Het
Anapc1 A G 2: 128,674,602 V404A probably benign Het
Bst2 A G 8: 71,537,282 F49S possibly damaging Het
C1qtnf9 G C 14: 60,779,570 W183S probably benign Het
Camsap3 A G 8: 3,598,087 I132V possibly damaging Het
Cchcr1 T A 17: 35,517,941 probably null Het
Cenpk A G 13: 104,230,748 K31E probably benign Het
Cfap221 T C 1: 119,925,571 K798E possibly damaging Het
Copb2 T A 9: 98,581,280 probably null Het
Dennd1a T A 2: 38,048,792 Y102F probably damaging Het
Eif2ak4 C T 2: 118,405,051 P88S probably damaging Het
Epha6 C A 16: 59,682,668 V959F probably damaging Het
Fam193a C A 5: 34,465,821 T1251K possibly damaging Het
Herc1 A T 9: 66,481,889 Q3896L probably benign Het
Ikbip C A 10: 91,083,228 D34E probably benign Het
Insr A G 8: 3,258,481 V185A probably benign Het
Jakmip1 C T 5: 37,174,765 Q930* probably null Het
Klc1 A G 12: 111,776,865 I209V probably benign Het
Lrrk2 T A 15: 91,764,782 L1660M probably damaging Het
Mbd3l1 G T 9: 18,484,914 D112Y possibly damaging Het
Mrps2 T A 2: 28,468,246 V16E probably benign Het
Ncoa1 G A 12: 4,322,978 T141I possibly damaging Het
Ndst2 G T 14: 20,729,843 R110S probably damaging Het
Nlrp2 A G 7: 5,328,617 V260A probably damaging Het
Olfr1425 A G 19: 12,074,212 I140T probably benign Het
Olfr491 A G 7: 108,317,752 N286S probably damaging Het
Olfr575 A T 7: 102,955,253 V116E probably damaging Het
Pah T C 10: 87,570,286 V262A probably damaging Het
Pcnt T G 10: 76,369,904 E2538A probably damaging Het
Pdxk T A 10: 78,446,976 I162F probably damaging Het
Plod2 T C 9: 92,573,597 F110L probably damaging Het
Pm20d2 A T 4: 33,187,186 L154Q probably damaging Het
Podxl2 C T 6: 88,843,584 V445I possibly damaging Het
Ppp1r3a A T 6: 14,719,236 W560R probably benign Het
Rasal3 A G 17: 32,392,709 S815P probably damaging Het
Rdm1 A G 11: 101,633,828 K196E probably damaging Het
Rsf1 CG CGACGGCGGGG 7: 97,579,908 probably benign Het
Scn1a T C 2: 66,350,942 D79G possibly damaging Het
Slc22a21 A G 11: 53,979,503 Y119H possibly damaging Het
Stip1 C T 19: 7,021,810 G467S possibly damaging Het
Synrg C A 11: 84,039,672 A1280E possibly damaging Het
Szt2 A G 4: 118,375,479 C2396R unknown Het
Trappc3 A G 4: 126,273,933 N95S probably benign Het
Tulp4 C T 17: 6,231,780 H695Y probably damaging Het
Ush2a T A 1: 188,381,484 D633E probably benign Het
Wasl G A 6: 24,633,187 P151S probably benign Het
Wwp2 A G 8: 107,483,356 N122S probably benign Het
Zfp51 A G 17: 21,463,569 R149G possibly damaging Het
Zfp764 A G 7: 127,404,715 S415P possibly damaging Het
Other mutations in Olfr828
AlleleSourceChrCoordTypePredicted EffectPPH Score
IGL02088:Olfr828 APN 9 18815923 missense probably benign 0.03
IGL02103:Olfr828 APN 9 18815709 missense probably damaging 1.00
IGL02792:Olfr828 APN 9 18815958 missense probably benign 0.00
IGL02964:Olfr828 APN 9 18815728 missense probably damaging 1.00
IGL03087:Olfr828 APN 9 18816084 missense probably damaging 1.00
IGL03105:Olfr828 APN 9 18815389 missense probably benign 0.03
R0330:Olfr828 UTSW 9 18815641 missense probably damaging 1.00
R0335:Olfr828 UTSW 9 18815994 missense probably damaging 1.00
R0862:Olfr828 UTSW 9 18815706 missense probably damaging 0.98
R1226:Olfr828 UTSW 9 18815970 missense probably benign 0.34
R2004:Olfr828 UTSW 9 18815505 missense probably benign 0.05
R2005:Olfr828 UTSW 9 18815505 missense probably benign 0.05
R2006:Olfr828 UTSW 9 18815505 missense probably benign 0.05
R2199:Olfr828 UTSW 9 18815923 missense probably damaging 0.97
R2230:Olfr828 UTSW 9 18815725 missense probably damaging 1.00
R2399:Olfr828 UTSW 9 18816027 missense probably benign 0.07
R5652:Olfr828 UTSW 9 18815626 missense probably damaging 1.00
R5738:Olfr828 UTSW 9 18815829 missense possibly damaging 0.81
R6416:Olfr828 UTSW 9 18815892 missense probably benign 0.21
R6813:Olfr828 UTSW 9 18815892 missense probably benign 0.21
R7092:Olfr828 UTSW 9 18816057 missense probably damaging 1.00
R7292:Olfr828 UTSW 9 18816190 missense probably damaging 1.00
R7429:Olfr828 UTSW 9 18815354 makesense probably null
R7430:Olfr828 UTSW 9 18815354 makesense probably null
R7490:Olfr828 UTSW 9 18815933 nonsense probably null
R7835:Olfr828 UTSW 9 18815809 missense probably benign 0.05
R7918:Olfr828 UTSW 9 18815809 missense probably benign 0.05
R8016:Olfr828 UTSW 9 18816292 start codon destroyed probably null 0.56
RF003:Olfr828 UTSW 9 18815482 missense probably benign 0.03
X0026:Olfr828 UTSW 9 18815763 missense possibly damaging 0.95
Z1176:Olfr828 UTSW 9 18815980 frame shift probably null
Z1177:Olfr828 UTSW 9 18816148 missense probably benign 0.09
Predicted Primers PCR Primer
(F):5'- CAAGGCTACCTCCATGTCTCTG -3'
(R):5'- GGTTGTCCTTCTGCACAAAC -3'

Sequencing Primer
(F):5'- ATAAAGGGATTCAACATTTGTGGGAC -3'
(R):5'- GTCCTTCTGCACAAACTTAGAAATC -3'
Posted On2019-05-15