Mutagenetix > Incidental Mutation

Incidental Mutation 'R7110:Vmn2r28'

551446

Institutional Source

Beutler Lab

Gene Symbol

Vmn2r28

Ensembl Gene

ENSMUSG00000066820

Gene Name

vomeronasal 2, receptor 28

Synonyms

EG665255

MMRRC Submission

045202-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.113) question?

Stock #

R7110 (G1)

Quality Score

225.009

Status

Validated

Chromosome

Chromosomal Location

5479531-5493967 bp(-) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

T to G at 5490734 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Asparagine to Threonine at position 71 (N71T)

Ref Sequence

ENSEMBL: ENSMUSP00000083477 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000086297]

AlphaFold

L7N203

Predicted Effect

probably benign
Transcript: ENSMUST00000086297
AA Change: N71T

PolyPhen 2 Score 0.057 (Sensitivity: 0.94; Specificity: 0.84)

SMART Domains

Protein: ENSMUSP00000083477
Gene: ENSMUSG00000066820
AA Change: N71T

Domain	Start	End	E-Value	Type
signal peptide	1	18	N/A	INTRINSIC
Pfam:ANF_receptor	73	470	1.7e-26	PFAM
Pfam:NCD3G	512	565	9.8e-21	PFAM
Pfam:7tm_3	598	833	2.8e-56	PFAM

Coding Region Coverage

1x: 100.0%
3x: 99.9%
10x: 99.7%
20x: 99.0%

Validation Efficiency

100% (62/62)

Allele List at MGI

Other mutations in this stock

Total: 64 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
1810065E05Rik	A	G	11: 58,425,745	T184A	possibly damaging	Het
Abca4	T	A	3: 122,132,643	Y1243N	probably damaging	Het
Adamts7	A	G	9: 90,193,964	T1250A	possibly damaging	Het
Adgrg3	T	C	8: 95,034,963	V118A	possibly damaging	Het
Agrn	A	G	4: 156,178,875	V364A	possibly damaging	Het
Cacna2d1	C	A	5: 16,357,784	L853I	probably damaging	Het
Ccdc25	A	G	14: 65,856,716	K124R	probably benign	Het
Ccdc73	A	G	2: 104,973,224	M236V	probably benign	Het
Cdh1	G	A	8: 106,668,544	D862N	possibly damaging	Het
Cdh5	A	G	8: 104,140,768	D559G	probably damaging	Het
Celsr2	C	A	3: 108,397,865	G2133C	probably damaging	Het
Chd5	A	G	4: 152,385,439	N1823S	probably damaging	Het
Cog7	T	C	7: 121,935,776	N562S	probably damaging	Het
Dcaf6	A	T	1: 165,351,968	S534R	probably benign	Het
Donson	G	A	16: 91,682,121	R436*	probably null	Het
Fam53c	T	A	18: 34,762,470		probably null	Het
Foxi3	A	G	6: 70,960,746	T321A	probably benign	Het
Frmd4b	A	T	6: 97,296,231	Y733*	probably null	Het
Fscn2	A	T	11: 120,366,754	T314S	probably benign	Het
Gfral	A	G	9: 76,164,830	I386T	possibly damaging	Het
Gm21698	T	C	5: 25,985,177	E174G	probably damaging	Het
Gm40460	CACAGCCTCCCTTGCAGCCCCCACAGGAACTACAGCCTCCCTTGCAGCCCCCACAGGAACTACAGCCTCCCTTGCAGCCCCCACAGGAACTACAGCCTCCCTTGCAGCCCCCACAG	CACAGCCTCCCTTGCAGCCCCCACAGGAACTACAGCCTCCCTTGCAGCCCCCACAGGAACTACAGCCTCCCTTGCAGCCCCCACAG	7: 142,240,817		probably benign	Het
Gm9573	CGGGGTGGGTGTAGATCCTGAGGCAGTGCTGGATACAGGGGTGGTTGGGGTGGGTGAAGAGCCTGAGGCAGTGCTGGATGCAGGGGTGGTCGGGGTAGGTGTAGATCCTGAGGCAGTGCT	CGGGGTGGGTGAAGAGCCTGAGGCAGTGCTGGATGCAGGGGTGGTCGGGGTAGGTGTAGATCCTGAGGCAGTGCT	17: 35,622,618		probably benign	Het
Gprin1	T	C	13: 54,739,243	D406G	probably benign	Het
Habp2	A	T	19: 56,311,164	R128*	probably null	Het
Hoxc10	A	T	15: 102,970,921	Y292F	probably damaging	Het
Hydin	A	T	8: 110,354,951		probably null	Het
Igkv12-89	T	C	6: 68,835,131	D18G	probably damaging	Het
Jhy	T	C	9: 40,917,260	N450S	probably damaging	Het
Klhl18	T	A	9: 110,450,765	Q119L	probably damaging	Het
Krt1	AAGCTGCCACCCCCAAAGCCACCACCGCCGTAGCTGCCACCCCCAAAGCCACCACCGCCGTAGCTGCCACCCCCAAAGCCACCAC	AAGCTGCCACCCCCAAAGCCACCACCGCCGTAGCTGCCACCCCCAAAGCCACCAC	15: 101,850,378		probably benign	Het
Lsmem1	A	T	12: 40,185,273		probably null	Het
Ly75	A	G	2: 60,376,184	I47T	probably benign	Het
Med12l	C	A	3: 59,262,224	T1603K	possibly damaging	Het
Mgat5	A	G	1: 127,382,979	D210G	possibly damaging	Het
Mgmt	G	T	7: 137,085,986	G55W	probably damaging	Het
Mrpl57	G	A	14: 57,826,297		probably benign	Het
Mtg1	G	A	7: 140,146,866	R209Q	probably benign	Het
Muc5ac	T	G	7: 141,799,822	C826W	possibly damaging	Het
Myot	A	G	18: 44,341,386	D146G	probably damaging	Het
Nlrp4f	T	A	13: 65,199,346	I11F	probably damaging	Het
Nsun5	T	A	5: 135,371,250	Y76N	probably damaging	Het
Olfr8	A	T	10: 78,955,450	M82L	possibly damaging	Het
Pcdhb3	A	G	18: 37,302,922	N647S	possibly damaging	Het
Pdgfra	T	A	5: 75,189,234	Y926*	probably null	Het
Pdzd2	A	T	15: 12,368,013	L2630H	probably damaging	Het
Phox2b	G	A	5: 67,096,162	S297L	unknown	Het
Polr3e	T	A	7: 120,940,287		probably null	Het
Ppp4r2	T	A	6: 100,865,862	V238E	probably damaging	Het
Proc	A	T	18: 32,133,388	F129I	probably benign	Het
Sgca	T	A	11: 94,963,401		probably null	Het
Slc7a10	A	G	7: 35,199,584	H360R	probably benign	Het
Slurp2	G	A	15: 74,743,115	T59I	probably benign	Het
Son	A	G	16: 91,656,518	T718A	probably benign	Het
Sorbs1	G	C	19: 40,376,800	R180G	probably benign	Het
Spink7	T	C	18: 62,594,267	N62S	probably damaging	Het
Stxbp3	C	A	3: 108,816,333	R195S	probably damaging	Het
Sulf1	G	A	1: 12,838,601	V613M	probably damaging	Het
Tbc1d9b	A	T	11: 50,163,830	I934F	probably benign	Het
Tecpr2	T	G	12: 110,918,972	L195R	probably damaging	Het
Tns2	G	C	15: 102,105,366	C71S	probably damaging	Het
Tulp4	C	T	17: 6,231,780	H695Y	probably damaging	Het
Usp40	G	T	1: 87,986,162	T403K	probably benign	Het
Vat1	G	T	11: 101,465,713	R141S	possibly damaging	Het

Other mutations in Vmn2r28

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00095:Vmn2r28	APN	7	5488069	missense	probably benign	0.12
IGL01061:Vmn2r28	APN	7	5488184	missense	probably damaging	1.00
IGL01160:Vmn2r28	APN	7	5486478	missense	probably damaging	0.99
IGL01335:Vmn2r28	APN	7	5481088	missense	possibly damaging	0.67
IGL01532:Vmn2r28	APN	7	5486464	missense	probably benign	0.16
IGL01791:Vmn2r28	APN	7	5488162	missense	probably benign	0.00
IGL01875:Vmn2r28	APN	7	5481303	missense	probably benign	0.30
IGL02161:Vmn2r28	APN	7	5488124	missense	possibly damaging	0.88
IGL02499:Vmn2r28	APN	7	5490569	missense	probably damaging	0.98
IGL02858:Vmn2r28	APN	7	5481004	missense	probably damaging	0.99
IGL03061:Vmn2r28	APN	7	5484016	missense	probably damaging	0.98
G1patch:Vmn2r28	UTSW	7	5488409	missense	probably benign	0.13
R0288:Vmn2r28	UTSW	7	5488021	missense	probably damaging	1.00
R0361:Vmn2r28	UTSW	7	5493716	missense	probably benign	0.00
R0396:Vmn2r28	UTSW	7	5488514	missense	probably benign	0.05
R0480:Vmn2r28	UTSW	7	5490457	missense	probably benign	0.00
R0485:Vmn2r28	UTSW	7	5488690	missense	probably damaging	1.00
R0837:Vmn2r28	UTSW	7	5488027	missense	probably damaging	0.99
R1282:Vmn2r28	UTSW	7	5481302	missense	probably damaging	0.99
R1296:Vmn2r28	UTSW	7	5481545	missense	possibly damaging	0.81
R1829:Vmn2r28	UTSW	7	5493811	missense	probably benign
R1853:Vmn2r28	UTSW	7	5481247	nonsense	probably null
R1869:Vmn2r28	UTSW	7	5486346	missense	probably benign	0.00
R1887:Vmn2r28	UTSW	7	5488289	missense	possibly damaging	0.90
R1961:Vmn2r28	UTSW	7	5481071	missense	possibly damaging	0.85
R1998:Vmn2r28	UTSW	7	5488314	missense	possibly damaging	0.87
R2392:Vmn2r28	UTSW	7	5484131	missense	probably damaging	0.98
R2432:Vmn2r28	UTSW	7	5488702	missense	probably damaging	0.99
R3055:Vmn2r28	UTSW	7	5481392	missense	probably damaging	0.98
R3753:Vmn2r28	UTSW	7	5488027	missense	probably damaging	0.99
R3877:Vmn2r28	UTSW	7	5488358	missense	probably damaging	1.00
R4307:Vmn2r28	UTSW	7	5490708	missense	probably damaging	0.99
R5023:Vmn2r28	UTSW	7	5486464	missense	probably benign	0.16
R5057:Vmn2r28	UTSW	7	5486464	missense	probably benign	0.16
R5083:Vmn2r28	UTSW	7	5480672	missense	possibly damaging	0.77
R5427:Vmn2r28	UTSW	7	5486377	missense	probably damaging	0.99
R5472:Vmn2r28	UTSW	7	5487944	critical splice donor site	probably null
R5511:Vmn2r28	UTSW	7	5484012	missense	possibly damaging	0.61
R5731:Vmn2r28	UTSW	7	5488669	missense	probably benign	0.41
R6091:Vmn2r28	UTSW	7	5493791	missense	possibly damaging	0.88
R6179:Vmn2r28	UTSW	7	5488004	nonsense	probably null
R6276:Vmn2r28	UTSW	7	5490731	missense	probably benign	0.01
R6441:Vmn2r28	UTSW	7	5488475	missense	probably benign	0.00
R6463:Vmn2r28	UTSW	7	5486436	missense	probably benign	0.07
R6528:Vmn2r28	UTSW	7	5490685	missense	probably benign	0.12
R6725:Vmn2r28	UTSW	7	5488409	missense	probably benign	0.13
R6760:Vmn2r28	UTSW	7	5481230	missense	probably damaging	0.97
R6849:Vmn2r28	UTSW	7	5480807	missense	probably damaging	1.00
R7146:Vmn2r28	UTSW	7	5481496	missense	probably benign	0.05
R7407:Vmn2r28	UTSW	7	5481309	missense	probably damaging	1.00
R7563:Vmn2r28	UTSW	7	5488201	missense	probably benign	0.00
R7611:Vmn2r28	UTSW	7	5481256	missense	probably benign	0.02
R7808:Vmn2r28	UTSW	7	5493679	missense	probably damaging	0.98
R7862:Vmn2r28	UTSW	7	5490614	missense	probably benign	0.00
R7916:Vmn2r28	UTSW	7	5480819	missense	probably damaging	1.00
R8183:Vmn2r28	UTSW	7	5488148	missense	probably damaging	1.00
R8334:Vmn2r28	UTSW	7	5484060	missense	probably damaging	1.00
R8519:Vmn2r28	UTSW	7	5486348	missense	probably benign	0.31
R8989:Vmn2r28	UTSW	7	5490474	missense	probably benign	0.00
R9066:Vmn2r28	UTSW	7	5488597	missense	probably damaging	1.00
R9422:Vmn2r28	UTSW	7	5480748	missense	probably damaging	1.00
R9469:Vmn2r28	UTSW	7	5484119	missense	probably damaging	0.99
R9608:Vmn2r28	UTSW	7	5488221	missense	probably benign	0.05

Predicted Primers

PCR Primer
(F):5'- CGGGTCCTGTAAGCAGAAATG -3'
(R):5'- TTCTCTGTGGATGAGGTCAATAC -3'

Sequencing Primer
(F):5'- GCAGAAATGAACACATAGTCTCTTC -3'
(R):5'- TGGGAGAAATACTGTCATGAAATTG -3'

Posted On

2019-05-15