Mutagenetix > Incidental Mutation

Incidental Mutation 'R7115:Fry'

551721

Institutional Source

Beutler Lab

Gene Symbol

Fry

Ensembl Gene

ENSMUSG00000056602

Gene Name

FRY microtubule binding protein

Synonyms

9330186A19Rik, cg003

MMRRC Submission

045206-MU

Accession Numbers

Essential gene?

Possibly essential (E-score: 0.585) question?

Stock #

R7115 (G1)

Quality Score

225.009

Status

Not validated

Chromosome

Chromosomal Location

150118645-150497753 bp(+) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

A to T at 150386067 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Arginine to Tryptophan at position 659 (R659W)

Ref Sequence

ENSEMBL: ENSMUSP00000084454 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000087204]

AlphaFold

no structure available at present

Predicted Effect

probably damaging
Transcript: ENSMUST00000087204
AA Change: R659W

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)

SMART Domains

Protein: ENSMUSP00000084454
Gene: ENSMUSG00000056602
AA Change: R659W

Domain	Start	End	E-Value	Type
Pfam:MOR2-PAG1_N	165	697	5.3e-170	PFAM
low complexity region	1014	1040	N/A	INTRINSIC
Pfam:MOR2-PAG1_mid	1188	1380	1.2e-15	PFAM
Pfam:MOR2-PAG1_mid	1398	1534	1.5e-5	PFAM
Pfam:MOR2-PAG1_mid	1632	1704	1.8e-7	PFAM
Pfam:MOR2-PAG1_mid	1772	1906	4.9e-10	PFAM
low complexity region	1936	1956	N/A	INTRINSIC
low complexity region	1962	1980	N/A	INTRINSIC
Pfam:MOR2-PAG1_C	2050	2303	1.9e-74	PFAM
low complexity region	2369	2385	N/A	INTRINSIC
low complexity region	2463	2482	N/A	INTRINSIC
low complexity region	2525	2534	N/A	INTRINSIC
low complexity region	2836	2852	N/A	INTRINSIC

Coding Region Coverage

1x: 100.0%
3x: 99.9%
10x: 99.7%
20x: 99.0%

Validation Efficiency

Allele List at MGI

Other mutations in this stock

Total: 36 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Abcc1	T	A	16: 14,437,725 (GRCm38)	I636N	probably benign	Het
Adam5	A	G	8: 24,781,696 (GRCm38)	I565T	possibly damaging	Het
Amd2	C	A	10: 35,711,637 (GRCm38)		probably benign	Het
Ap5m1	T	A	14: 49,086,270 (GRCm38)	Y472*	probably null	Het
Apcdd1	T	C	18: 62,936,953 (GRCm38)	F97S	probably damaging	Het
Axdnd1	T	C	1: 156,380,876 (GRCm38)	K267R		Het
BC067074	T	G	13: 113,320,776 (GRCm38)	S1119A		Het
Carf	G	T	1: 60,148,150 (GRCm38)	L637F	probably damaging	Het
Ccdc103	A	T	11: 102,883,810 (GRCm38)	S95C	possibly damaging	Het
Cfap20	T	C	8: 95,421,246 (GRCm38)	I156V	probably benign	Het
Csn1s2a	T	C	5: 87,781,805 (GRCm38)	C96R	probably benign	Het
Ctif	CGGGGCACACTTTGCTCTTACCTCCCGGAGGCACGTGTAGATGGGGCACACTTTGCTCTTACCTCCCGGAGGCACGTGTAGATGGGGCACAC	CGGGGCACACTTTGCTCTTACCTCCCGGAGGCACGTGTAGATGGGGCACAC	18: 75,471,803 (GRCm38)		probably benign	Het
Dennd2a	G	A	6: 39,506,711 (GRCm38)	T405M	probably damaging	Het
Dennd5a	A	G	7: 109,894,754 (GRCm38)	Y1248H	probably damaging	Het
Dnah14	A	T	1: 181,720,145 (GRCm38)	K2504N	probably damaging	Het
Dnajb2	G	A	1: 75,243,662 (GRCm38)	G275D		Het
Elf3	T	G	1: 135,257,118 (GRCm38)	D110A	probably damaging	Het
Eomes	A	G	9: 118,484,489 (GRCm38)	N534S	probably benign	Het
Gm10428	G	A	11: 62,753,380 (GRCm38)	C94Y	unknown	Het
Gm2696	T	A	10: 77,836,299 (GRCm38)	C111S	unknown	Het
Gpt2	G	A	8: 85,518,052 (GRCm38)	E325K	probably benign	Het
Lrp1b	T	A	2: 40,998,235 (GRCm38)	N2338Y		Het
Map2k1	A	G	9: 64,212,606 (GRCm38)	I139T	probably damaging	Het
Olfr44	T	C	9: 39,484,648 (GRCm38)	I202V	probably benign	Het
Olfr964-ps1	T	C	9: 39,686,707 (GRCm38)	Y79C	probably benign	Het
Pxylp1	G	A	9: 96,825,010 (GRCm38)	T373I	probably benign	Het
Ranbp9	T	C	13: 43,406,671 (GRCm38)	N484S	probably benign	Het
Rassf5	A	G	1: 131,181,249 (GRCm38)	V293A	probably benign	Het
Ring1	A	G	17: 34,023,446 (GRCm38)	I29T	probably damaging	Het
Scn1a	G	A	2: 66,324,618 (GRCm38)	Q666*	probably null	Het
Spice1	G	A	16: 44,379,275 (GRCm38)	G697R	probably benign	Het
Tas2r131	A	G	6: 132,957,604 (GRCm38)	F81L	probably benign	Het
Tenm2	G	A	11: 36,163,817 (GRCm38)	S572L	probably damaging	Het
Tfr2	A	G	5: 137,571,715 (GRCm38)	T128A	probably benign	Het
Trim56	T	A	5: 137,113,660 (GRCm38)	Q334L	probably damaging	Het
Vmn2r44	T	A	7: 8,367,528 (GRCm38)	R840*	probably null	Het

Other mutations in Fry

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00327:Fry	APN	5	150,340,404 (GRCm38)	nonsense	probably null
IGL00328:Fry	APN	5	150,340,404 (GRCm38)	nonsense	probably null
IGL00841:Fry	APN	5	150,422,724 (GRCm38)	missense	probably benign
IGL00938:Fry	APN	5	150,370,180 (GRCm38)	missense	probably damaging	1.00
IGL01015:Fry	APN	5	150,422,787 (GRCm38)	missense	probably benign	0.18
IGL01401:Fry	APN	5	150,438,788 (GRCm38)	missense	probably benign
IGL01616:Fry	APN	5	150,399,599 (GRCm38)	missense	probably damaging	1.00
IGL01616:Fry	APN	5	150,438,811 (GRCm38)	splice site	probably null
IGL01748:Fry	APN	5	150,345,651 (GRCm38)	splice site	probably benign
IGL01965:Fry	APN	5	150,381,621 (GRCm38)	missense	probably damaging	1.00
IGL02030:Fry	APN	5	150,471,618 (GRCm38)	splice site	probably benign
IGL02079:Fry	APN	5	150,399,624 (GRCm38)	missense	probably damaging	0.97
IGL02087:Fry	APN	5	150,403,594 (GRCm38)	missense	probably benign	0.23
IGL02113:Fry	APN	5	150,399,605 (GRCm38)	missense	probably benign
IGL02209:Fry	APN	5	150,437,026 (GRCm38)	missense	probably benign	0.00
IGL02250:Fry	APN	5	150,403,434 (GRCm38)	splice site	probably benign
IGL02265:Fry	APN	5	150,437,153 (GRCm38)	missense	probably damaging	1.00
IGL02486:Fry	APN	5	150,491,177 (GRCm38)	missense	probably damaging	0.99
IGL02552:Fry	APN	5	150,380,910 (GRCm38)	missense	probably damaging	1.00
IGL02881:Fry	APN	5	150,359,051 (GRCm38)	missense	probably damaging	0.99
IGL03008:Fry	APN	5	150,345,556 (GRCm38)	missense	possibly damaging	0.82
IGL03140:Fry	APN	5	150,495,701 (GRCm38)	missense	probably damaging	0.98
IGL03171:Fry	APN	5	150,380,809 (GRCm38)	missense	probably damaging	1.00
IGL03389:Fry	APN	5	150,394,231 (GRCm38)	missense	probably damaging	1.00
IGL03404:Fry	APN	5	150,326,168 (GRCm38)	missense	probably damaging	1.00
Brook	UTSW	5	150,326,132 (GRCm38)	missense	probably damaging	1.00
haydn	UTSW	5	150,418,464 (GRCm38)	missense	possibly damaging	0.94
miracle	UTSW	5	150,437,159 (GRCm38)	missense	probably damaging	0.99
quickening	UTSW	5	150,434,776 (GRCm38)	missense	probably damaging	1.00
seasons	UTSW	5	150,466,437 (GRCm38)	missense	probably benign	0.06
Vivaldi	UTSW	5	150,394,138 (GRCm38)	missense	possibly damaging	0.80
R0023:Fry	UTSW	5	150,451,098 (GRCm38)	missense	possibly damaging	0.78
R0024:Fry	UTSW	5	150,380,803 (GRCm38)	missense	probably benign	0.03
R0030:Fry	UTSW	5	150,372,569 (GRCm38)	nonsense	probably null
R0053:Fry	UTSW	5	150,461,377 (GRCm38)	splice site	probably benign
R0089:Fry	UTSW	5	150,340,427 (GRCm38)	missense	possibly damaging	0.91
R0212:Fry	UTSW	5	150,496,397 (GRCm38)	missense	probably damaging	0.99
R0241:Fry	UTSW	5	150,260,346 (GRCm38)	intron	probably benign
R0265:Fry	UTSW	5	150,434,776 (GRCm38)	missense	probably damaging	1.00
R0317:Fry	UTSW	5	150,471,468 (GRCm38)	missense	probably damaging	1.00
R0532:Fry	UTSW	5	150,478,761 (GRCm38)	splice site	probably benign
R0532:Fry	UTSW	5	150,433,707 (GRCm38)	unclassified	probably benign
R0599:Fry	UTSW	5	150,437,159 (GRCm38)	missense	probably damaging	0.99
R0631:Fry	UTSW	5	150,496,352 (GRCm38)	missense	possibly damaging	0.82
R0723:Fry	UTSW	5	150,496,360 (GRCm38)	missense	probably damaging	1.00
R0766:Fry	UTSW	5	150,403,432 (GRCm38)	splice site	probably benign
R0790:Fry	UTSW	5	150,466,437 (GRCm38)	missense	probably benign	0.06
R0928:Fry	UTSW	5	150,437,084 (GRCm38)	missense	probably damaging	1.00
R1104:Fry	UTSW	5	150,496,289 (GRCm38)	missense	probably damaging	1.00
R1144:Fry	UTSW	5	150,418,464 (GRCm38)	missense	possibly damaging	0.94
R1172:Fry	UTSW	5	150,481,494 (GRCm38)	nonsense	probably null
R1312:Fry	UTSW	5	150,403,432 (GRCm38)	splice site	probably benign
R1347:Fry	UTSW	5	150,495,818 (GRCm38)	missense	probably damaging	1.00
R1347:Fry	UTSW	5	150,495,818 (GRCm38)	missense	probably damaging	1.00
R1437:Fry	UTSW	5	150,310,425 (GRCm38)	missense	possibly damaging	0.92
R1458:Fry	UTSW	5	150,380,859 (GRCm38)	missense	probably damaging	1.00
R1542:Fry	UTSW	5	150,404,966 (GRCm38)	missense	probably benign	0.13
R1692:Fry	UTSW	5	150,370,227 (GRCm38)	missense	probably damaging	1.00
R1826:Fry	UTSW	5	150,436,709 (GRCm38)	missense	possibly damaging	0.82
R1874:Fry	UTSW	5	150,345,921 (GRCm38)	missense	probably damaging	1.00
R1875:Fry	UTSW	5	150,326,132 (GRCm38)	missense	probably damaging	1.00
R1881:Fry	UTSW	5	150,478,046 (GRCm38)	missense	probably damaging	0.97
R1884:Fry	UTSW	5	150,403,520 (GRCm38)	missense	probably benign	0.00
R1929:Fry	UTSW	5	150,400,924 (GRCm38)	missense	probably null	0.02
R2066:Fry	UTSW	5	150,370,119 (GRCm38)	splice site	probably benign
R2270:Fry	UTSW	5	150,400,924 (GRCm38)	missense	probably null	0.02
R2356:Fry	UTSW	5	150,471,432 (GRCm38)	missense	probably benign
R3720:Fry	UTSW	5	150,454,572 (GRCm38)	missense	probably damaging	1.00
R3773:Fry	UTSW	5	150,398,198 (GRCm38)	missense	probably damaging	0.96
R3824:Fry	UTSW	5	150,496,419 (GRCm38)	missense	possibly damaging	0.94
R3902:Fry	UTSW	5	150,345,927 (GRCm38)	missense	probably damaging	1.00
R3923:Fry	UTSW	5	150,413,349 (GRCm38)	missense	probably benign
R4250:Fry	UTSW	5	150,310,360 (GRCm38)	missense	probably damaging	0.99
R4332:Fry	UTSW	5	150,381,663 (GRCm38)	missense	probably damaging	1.00
R4495:Fry	UTSW	5	150,310,463 (GRCm38)	missense	probably damaging	1.00
R4610:Fry	UTSW	5	150,386,104 (GRCm38)	missense	probably damaging	1.00
R4682:Fry	UTSW	5	150,422,754 (GRCm38)	missense	probably damaging	1.00
R4732:Fry	UTSW	5	150,386,007 (GRCm38)	missense
R4733:Fry	UTSW	5	150,386,007 (GRCm38)	missense
R4755:Fry	UTSW	5	150,398,254 (GRCm38)	missense	probably damaging	0.99
R4788:Fry	UTSW	5	150,399,636 (GRCm38)	missense	probably benign	0.00
R4803:Fry	UTSW	5	150,399,533 (GRCm38)	missense	probably benign	0.31
R4858:Fry	UTSW	5	150,401,643 (GRCm38)	missense	possibly damaging	0.78
R4872:Fry	UTSW	5	150,394,239 (GRCm38)	critical splice donor site	probably null
R4902:Fry	UTSW	5	150,495,703 (GRCm38)	missense	probably benign	0.43
R4915:Fry	UTSW	5	150,478,863 (GRCm38)	missense	probably benign	0.30
R4938:Fry	UTSW	5	150,477,989 (GRCm38)	missense	probably damaging	1.00
R4983:Fry	UTSW	5	150,398,254 (GRCm38)	missense	probably damaging	1.00
R5004:Fry	UTSW	5	150,433,604 (GRCm38)	missense	probably benign	0.16
R5040:Fry	UTSW	5	150,388,854 (GRCm38)	missense	probably damaging	0.99
R5145:Fry	UTSW	5	150,370,224 (GRCm38)	missense	probably damaging	0.98
R5170:Fry	UTSW	5	150,429,854 (GRCm38)	missense	probably benign	0.03
R5233:Fry	UTSW	5	150,469,720 (GRCm38)	missense	possibly damaging	0.71
R5428:Fry	UTSW	5	150,405,359 (GRCm38)	missense	possibly damaging	0.89
R5468:Fry	UTSW	5	150,399,588 (GRCm38)	missense	probably benign	0.44
R5481:Fry	UTSW	5	150,260,319 (GRCm38)	missense	probably benign	0.01
R5494:Fry	UTSW	5	150,390,667 (GRCm38)	missense	probably damaging	1.00
R5538:Fry	UTSW	5	150,495,848 (GRCm38)	missense	probably damaging	1.00
R5638:Fry	UTSW	5	150,359,081 (GRCm38)	missense	possibly damaging	0.46
R5645:Fry	UTSW	5	150,380,867 (GRCm38)	missense	probably damaging	1.00
R5716:Fry	UTSW	5	150,370,221 (GRCm38)	nonsense	probably null
R5812:Fry	UTSW	5	150,399,671 (GRCm38)	missense	probably damaging	0.99
R5813:Fry	UTSW	5	150,399,671 (GRCm38)	missense	probably damaging	0.99
R5873:Fry	UTSW	5	150,378,885 (GRCm38)	missense	probably damaging	1.00
R5933:Fry	UTSW	5	150,390,800 (GRCm38)	intron	probably benign
R6037:Fry	UTSW	5	150,428,179 (GRCm38)	missense	probably benign	0.03
R6037:Fry	UTSW	5	150,428,179 (GRCm38)	missense	probably benign	0.03
R6158:Fry	UTSW	5	150,454,572 (GRCm38)	missense	probably damaging	1.00
R6178:Fry	UTSW	5	150,454,522 (GRCm38)	missense	probably damaging	1.00
R6481:Fry	UTSW	5	150,386,014 (GRCm38)	missense	probably damaging	1.00
R6562:Fry	UTSW	5	150,326,149 (GRCm38)	missense	probably damaging	1.00
R6676:Fry	UTSW	5	150,380,922 (GRCm38)	missense	probably benign	0.22
R6717:Fry	UTSW	5	150,496,312 (GRCm38)	missense	probably benign	0.00
R6828:Fry	UTSW	5	150,466,446 (GRCm38)	splice site	probably null
R6874:Fry	UTSW	5	150,437,303 (GRCm38)	missense	probably benign	0.00
R6930:Fry	UTSW	5	150,428,230 (GRCm38)	missense	probably benign	0.00
R6963:Fry	UTSW	5	150,457,844 (GRCm38)	missense	probably benign	0.17
R6965:Fry	UTSW	5	150,416,220 (GRCm38)	missense	possibly damaging	0.79
R7051:Fry	UTSW	5	150,395,169 (GRCm38)	missense	possibly damaging	0.93
R7085:Fry	UTSW	5	150,438,749 (GRCm38)	missense	probably benign	0.02
R7108:Fry	UTSW	5	150,491,090 (GRCm38)	missense
R7108:Fry	UTSW	5	150,395,786 (GRCm38)	missense	probably damaging	1.00
R7116:Fry	UTSW	5	150,395,869 (GRCm38)	critical splice donor site	probably null
R7197:Fry	UTSW	5	150,469,767 (GRCm38)	missense
R7256:Fry	UTSW	5	150,466,786 (GRCm38)	missense
R7318:Fry	UTSW	5	150,436,993 (GRCm38)	missense	probably damaging	0.98
R7323:Fry	UTSW	5	150,496,349 (GRCm38)	missense
R7358:Fry	UTSW	5	150,416,323 (GRCm38)	missense	probably benign
R7361:Fry	UTSW	5	150,436,847 (GRCm38)	missense	possibly damaging	0.92
R7395:Fry	UTSW	5	150,380,883 (GRCm38)	missense	possibly damaging	0.82
R7487:Fry	UTSW	5	150,414,574 (GRCm38)	missense	possibly damaging	0.79
R7491:Fry	UTSW	5	150,466,326 (GRCm38)	missense
R7574:Fry	UTSW	5	150,380,894 (GRCm38)	missense	probably benign	0.00
R7582:Fry	UTSW	5	150,496,382 (GRCm38)	missense
R7586:Fry	UTSW	5	150,426,218 (GRCm38)	missense	probably damaging	1.00
R7650:Fry	UTSW	5	150,413,418 (GRCm38)	missense	probably damaging	1.00
R7699:Fry	UTSW	5	150,405,327 (GRCm38)	missense	probably damaging	0.98
R7700:Fry	UTSW	5	150,405,327 (GRCm38)	missense	probably damaging	0.98
R7972:Fry	UTSW	5	150,310,396 (GRCm38)	missense	probably benign	0.05
R8058:Fry	UTSW	5	150,495,767 (GRCm38)	missense
R8070:Fry	UTSW	5	150,478,007 (GRCm38)	missense
R8159:Fry	UTSW	5	150,399,533 (GRCm38)	missense	probably benign	0.31
R8202:Fry	UTSW	5	150,431,737 (GRCm38)	missense	probably damaging	1.00
R8261:Fry	UTSW	5	150,445,907 (GRCm38)	missense	probably damaging	1.00
R8279:Fry	UTSW	5	150,496,261 (GRCm38)	missense
R8338:Fry	UTSW	5	150,359,051 (GRCm38)	missense	probably damaging	0.99
R8370:Fry	UTSW	5	150,395,819 (GRCm38)	missense	probably damaging	1.00
R8673:Fry	UTSW	5	150,395,111 (GRCm38)	missense	possibly damaging	0.91
R8786:Fry	UTSW	5	150,394,036 (GRCm38)	missense	probably benign	0.00
R8815:Fry	UTSW	5	150,394,138 (GRCm38)	missense	possibly damaging	0.80
R8847:Fry	UTSW	5	150,386,007 (GRCm38)	missense
R9023:Fry	UTSW	5	150,437,303 (GRCm38)	missense	probably benign	0.00
R9025:Fry	UTSW	5	150,295,808 (GRCm38)	intron	probably benign
R9125:Fry	UTSW	5	150,346,060 (GRCm38)	missense	probably damaging	0.97
R9172:Fry	UTSW	5	150,413,328 (GRCm38)	missense	probably benign
R9262:Fry	UTSW	5	150,381,644 (GRCm38)	missense	probably damaging	1.00
R9263:Fry	UTSW	5	150,399,263 (GRCm38)	missense	probably damaging	1.00
R9293:Fry	UTSW	5	150,495,832 (GRCm38)	missense
R9368:Fry	UTSW	5	150,477,938 (GRCm38)	missense
R9401:Fry	UTSW	5	150,378,938 (GRCm38)	missense	probably damaging	1.00
R9402:Fry	UTSW	5	150,436,853 (GRCm38)	missense	probably damaging	1.00
R9402:Fry	UTSW	5	150,433,696 (GRCm38)	missense	possibly damaging	0.91
R9420:Fry	UTSW	5	150,433,529 (GRCm38)	missense	possibly damaging	0.72
R9557:Fry	UTSW	5	150,466,316 (GRCm38)	missense
R9647:Fry	UTSW	5	150,369,519 (GRCm38)	missense	probably damaging	1.00
R9650:Fry	UTSW	5	150,445,910 (GRCm38)	missense	probably damaging	1.00
R9655:Fry	UTSW	5	150,438,786 (GRCm38)	missense	possibly damaging	0.90
R9664:Fry	UTSW	5	150,359,023 (GRCm38)	missense	probably damaging	0.98
R9668:Fry	UTSW	5	150,358,853 (GRCm38)	missense	probably damaging	1.00
R9732:Fry	UTSW	5	150,405,293 (GRCm38)	missense	probably benign	0.00
R9773:Fry	UTSW	5	150,399,263 (GRCm38)	missense	probably damaging	1.00
Z1177:Fry	UTSW	5	150,310,437 (GRCm38)	missense	possibly damaging	0.80

Predicted Primers

PCR Primer
(F):5'- TGTCTGAGTGCTAGAGCCTG -3'
(R):5'- CACATAATCACCTCTGAGTTTCTGATC -3'

Sequencing Primer
(F):5'- AGTGCTAGAGCCTGCCTGG -3'
(R):5'- CACCTCTGAGTTTCTGATCTTGTTGG -3'

Posted On

2019-05-15