Mutagenetix > Incidental Mutation

Incidental Mutation 'R7115:Tas2r131'

551723

Institutional Source

Beutler Lab

Gene Symbol

Tas2r131

Ensembl Gene

ENSMUSG00000057699

Gene Name

taste receptor, type 2, member 131

Synonyms

mGR31, Tas2r31, mt2r61, T2R31

MMRRC Submission

Accession Numbers

Is this an essential gene?

Non essential (E-score: 0.000) question?

Stock #

R7115 (G1)

Quality Score

225.009

Status

Not validated

Chromosome

Chromosomal Location

132956884-132957919 bp(-) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

A to G at 132957604 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Phenylalanine to Leucine at position 81 (F81L)

Ref Sequence

ENSEMBL: ENSMUSP00000080736 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000082085]

AlphaFold

Q7M708

Predicted Effect

probably benign
Transcript: ENSMUST00000082085
AA Change: F81L

PolyPhen 2 Score 0.067 (Sensitivity: 0.94; Specificity: 0.84)

SMART Domains

Protein: ENSMUSP00000080736
Gene: ENSMUSG00000057699
AA Change: F81L

Domain	Start	End	E-Value	Type
Pfam:TAS2R	1	293	1.2e-66	PFAM

Coding Region Coverage

1x: 100.0%
3x: 99.9%
10x: 99.7%
20x: 99.0%

Validation Efficiency

Allele List at MGI

Other mutations in this stock

Total: 36 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Abcc1	T	A	16: 14,437,725	I636N	probably benign	Het
Adam5	A	G	8: 24,781,696	I565T	possibly damaging	Het
Amd2	C	A	10: 35,711,637		probably benign	Het
Ap5m1	T	A	14: 49,086,270	Y472*	probably null	Het
Apcdd1	T	C	18: 62,936,953	F97S	probably damaging	Het
Axdnd1	T	C	1: 156,380,876	K267R		Het
BC067074	T	G	13: 113,320,776	S1119A		Het
Carf	G	T	1: 60,148,150	L637F	probably damaging	Het
Ccdc103	A	T	11: 102,883,810	S95C	possibly damaging	Het
Cfap20	T	C	8: 95,421,246	I156V	probably benign	Het
Csn1s2a	T	C	5: 87,781,805	C96R	probably benign	Het
Ctif	CGGGGCACACTTTGCTCTTACCTCCCGGAGGCACGTGTAGATGGGGCACACTTTGCTCTTACCTCCCGGAGGCACGTGTAGATGGGGCACAC	CGGGGCACACTTTGCTCTTACCTCCCGGAGGCACGTGTAGATGGGGCACAC	18: 75,471,803		probably benign	Het
Dennd2a	G	A	6: 39,506,711	T405M	probably damaging	Het
Dennd5a	A	G	7: 109,894,754	Y1248H	probably damaging	Het
Dnah14	A	T	1: 181,720,145	K2504N	probably damaging	Het
Dnajb2	G	A	1: 75,243,662	G275D		Het
Elf3	T	G	1: 135,257,118	D110A	probably damaging	Het
Eomes	A	G	9: 118,484,489	N534S	probably benign	Het
Fry	A	T	5: 150,386,067	R659W	probably damaging	Het
Gm10428	G	A	11: 62,753,380	C94Y	unknown	Het
Gm2696	T	A	10: 77,836,299	C111S	unknown	Het
Gpt2	G	A	8: 85,518,052	E325K	probably benign	Het
Lrp1b	T	A	2: 40,998,235	N2338Y		Het
Map2k1	A	G	9: 64,212,606	I139T	probably damaging	Het
Olfr44	T	C	9: 39,484,648	I202V	probably benign	Het
Olfr964-ps1	T	C	9: 39,686,707	Y79C	probably benign	Het
Pxylp1	G	A	9: 96,825,010	T373I	probably benign	Het
Ranbp9	T	C	13: 43,406,671	N484S	probably benign	Het
Rassf5	A	G	1: 131,181,249	V293A	probably benign	Het
Ring1	A	G	17: 34,023,446	I29T	probably damaging	Het
Scn1a	G	A	2: 66,324,618	Q666*	probably null	Het
Spice1	G	A	16: 44,379,275	G697R	probably benign	Het
Tenm2	G	A	11: 36,163,817	S572L	probably damaging	Het
Tfr2	A	G	5: 137,571,715	T128A	probably benign	Het
Trim56	T	A	5: 137,113,660	Q334L	probably damaging	Het
Vmn2r44	T	A	7: 8,367,528	R840*	probably null	Het

Other mutations in Tas2r131

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00592:Tas2r131	APN	6	132957196	missense	probably damaging	1.00
IGL00795:Tas2r131	APN	6	132957591	missense	possibly damaging	0.94
IGL01023:Tas2r131	APN	6	132957801	missense	probably damaging	1.00
IGL02439:Tas2r131	APN	6	132957769	missense	probably damaging	1.00
R0335:Tas2r131	UTSW	6	132957829	missense	probably benign
R0523:Tas2r131	UTSW	6	132957451	missense	possibly damaging	0.71
R1056:Tas2r131	UTSW	6	132957067	missense	possibly damaging	0.68
R1069:Tas2r131	UTSW	6	132957825	missense	probably benign	0.01
R4073:Tas2r131	UTSW	6	132957839	nonsense	probably null
R4719:Tas2r131	UTSW	6	132956973	missense	probably damaging	1.00
R5002:Tas2r131	UTSW	6	132957151	missense	probably benign	0.00
R5049:Tas2r131	UTSW	6	132957577	missense	probably damaging	1.00
R5635:Tas2r131	UTSW	6	132957608	missense	probably benign	0.27
R5905:Tas2r131	UTSW	6	132957676	missense	probably benign	0.02
R6028:Tas2r131	UTSW	6	132957676	missense	probably benign	0.02
R6229:Tas2r131	UTSW	6	132957022	missense	probably damaging	1.00
R7561:Tas2r131	UTSW	6	132956958	missense	probably benign	0.26
R7741:Tas2r131	UTSW	6	132957475	missense	possibly damaging	0.60
R7810:Tas2r131	UTSW	6	132957742	missense	probably benign	0.11
R8878:Tas2r131	UTSW	6	132957504	missense	probably damaging	1.00
R9506:Tas2r131	UTSW	6	132957061	missense	possibly damaging	0.94
X0025:Tas2r131	UTSW	6	132957538	missense	probably damaging	1.00
Z1177:Tas2r131	UTSW	6	132956997	missense	probably damaging	1.00

Predicted Primers

PCR Primer
(F):5'- CCAAATAGGAAGTGTTTCAAGCAG -3'
(R):5'- GCTGGGAAATATGTTCATTGGAC -3'

Sequencing Primer
(F):5'- CTCCACTTAAACCAGAGGAA -3'
(R):5'- TCATTGGACTGGCAAACTGC -3'

Posted On

2019-05-15