Mutagenetix > Incidental Mutation

Incidental Mutation 'R7121:Lama3'

552041

Institutional Source

Beutler Lab

Gene Symbol

Lama3

Ensembl Gene

ENSMUSG00000024421

Gene Name

laminin, alpha 3

Synonyms

[a]3B, nicein, 150kDa

MMRRC Submission

045210-MU

Accession Numbers

Essential gene?

Essential (E-score: 1.000) question?

Stock #

R7121 (G1)

Quality Score

225.009

Status

Validated

Chromosome

Chromosomal Location

12333819-12583013 bp(+) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

G to A at 12462782 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Alanine to Threonine at position 923 (A923T)

Ref Sequence

ENSEMBL: ENSMUSP00000089703 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000092070]

AlphaFold

no structure available at present

Predicted Effect

probably benign
Transcript: ENSMUST00000092070
AA Change: A923T

PolyPhen 2 Score 0.063 (Sensitivity: 0.94; Specificity: 0.84)

SMART Domains

Protein: ENSMUSP00000089703
Gene: ENSMUSG00000024421
AA Change: A923T

Domain	Start	End	E-Value	Type
signal peptide	1	31	N/A	INTRINSIC
LamNT	38	294	1.46e-153	SMART
EGF_Lam	296	350	1.39e-4	SMART
EGF_Lam	353	420	2.66e-10	SMART
EGF_Lam	423	464	3.51e-10	SMART
EGF_Lam	488	530	1.73e-9	SMART
EGF_Lam	533	576	3.81e-11	SMART
EGF_like	579	625	1.82e-1	SMART
EGF_Lam	628	678	5.15e-8	SMART
EGF_Lam	681	725	3.54e-6	SMART
low complexity region	768	781	N/A	INTRINSIC
EGF_Lam	1263	1306	3.15e-12	SMART
EGF_Lam	1309	1350	6.3e-3	SMART
EGF_Lam	1353	1399	1.49e-13	SMART
EGF_Lam	1402	1450	8.18e-11	SMART
LamB	1509	1638	4.34e-55	SMART
Pfam:Laminin_EGF	1647	1681	7.9e-5	PFAM
EGF_Lam	1684	1728	2.66e-10	SMART
EGF_Lam	1731	1781	7.81e-8	SMART
Pfam:Laminin_I	1836	2102	2.7e-93	PFAM
low complexity region	2185	2200	N/A	INTRINSIC
coiled coil region	2211	2238	N/A	INTRINSIC
LamG	2406	2566	1.67e-2	SMART
LamG	2614	2742	1.72e-17	SMART
LamG	2785	2900	3.96e-17	SMART
LamG	3005	3133	1.12e-34	SMART
LamG	3175	3308	3.41e-30	SMART

Coding Region Coverage

1x: 100.0%
3x: 99.9%
10x: 99.7%
20x: 98.9%

Validation Efficiency

99% (71/72)

MGI Phenotype

FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] The protein encoded by this gene belongs to the laminin family of secreted molecules. Laminins are heterotrimeric molecules that consist of alpha, beta, and gamma subunits that assemble through a coiled-coil domain. Laminins are essential for formation and function of the basement membrane and have additional functions in regulating cell migration and mechanical signal transduction. This gene encodes an alpha subunit and is responsive to several epithelial-mesenchymal regulators including keratinocyte growth factor, epidermal growth factor and insulin-like growth factor. Mutations in this gene have been identified as the cause of Herlitz type junctional epidermolysis bullosa and laryngoonychocutaneous syndrome. Alternative splicing and alternative promoter usage result in multiple transcript variants. [provided by RefSeq, Dec 2014]
PHENOTYPE: Mice homozygous for a targeted null mutation develop a lethal blistering phenotype similar to human junctional epidermolysis bullosa, and die 2-3 days after birth from a failure to thrive. [provided by MGI curators]

Allele List at MGI

Other mutations in this stock

Total: 70 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
4933411K16Rik	C	T	19: 42,052,673 (GRCm38)	A81V	probably benign	Het
Abcc9	A	T	6: 142,689,127 (GRCm38)	L137*	probably null	Het
Akap10	A	G	11: 61,886,698 (GRCm38)		probably null	Het
Alms1	T	A	6: 85,624,622 (GRCm38)	Y1683N	probably damaging	Het
Atl1	A	G	12: 69,931,634 (GRCm38)	S127G	probably damaging	Het
Cadm1	G	A	9: 47,799,410 (GRCm38)	V204M	probably damaging	Het
Cbr3	T	A	16: 93,690,550 (GRCm38)	I207N	probably damaging	Het
Ccdc148	A	T	2: 58,827,567 (GRCm38)	Y475N	probably damaging	Het
Ccdc33	A	T	9: 58,080,884 (GRCm38)	S144T	probably benign	Het
Ceacam5	C	A	7: 17,745,537 (GRCm38)	A193E	probably benign	Het
Chd2	T	C	7: 73,469,670 (GRCm38)	D1042G	probably benign	Het
Chmp5	A	T	4: 40,952,217 (GRCm38)		probably null	Het
Clca1	T	A	3: 145,011,806 (GRCm38)	N467I	probably damaging	Het
D130040H23Rik	T	C	8: 69,302,279 (GRCm38)	V112A	probably damaging	Het
Dbh	A	T	2: 27,168,306 (GRCm38)	D162V	probably damaging	Het
Dnah12	G	A	14: 26,778,912 (GRCm38)		probably null	Het
Dnm2	C	T	9: 21,474,566 (GRCm38)	T295I	probably benign	Het
Faiml	A	C	9: 99,234,393 (GRCm38)	D81E	probably benign	Het
Fer1l4	T	A	2: 156,044,557 (GRCm38)	Y720F	probably benign	Het
Fn1	A	C	1: 71,600,538 (GRCm38)		probably benign	Het
Ftsj3	T	C	11: 106,252,297 (GRCm38)	E397G	probably damaging	Het
Gm16506	T	C	14: 43,727,360 (GRCm38)	K42E		Het
Gpr179	A	G	11: 97,334,730 (GRCm38)	S2200P	probably benign	Het
Gusb	T	C	5: 130,000,043 (GRCm38)	D202G	probably benign	Het
Hspe1	A	G	1: 55,089,151 (GRCm38)	E35G	probably damaging	Het
Kptn	T	A	7: 16,123,098 (GRCm38)	H170Q	probably damaging	Het
Lmo7	T	A	14: 101,887,035 (GRCm38)	I432K	probably damaging	Het
Maneal	G	A	4: 124,857,112 (GRCm38)	P284S	probably benign	Het
Mrgbp	A	G	2: 180,582,889 (GRCm38)	T28A	probably benign	Het
Myo1h	T	C	5: 114,338,229 (GRCm38)	V493A		Het
Naxd	T	C	8: 11,506,745 (GRCm38)	L122P	probably damaging	Het
Neto2	T	C	8: 85,670,391 (GRCm38)		probably null	Het
Obscn	T	A	11: 59,013,252 (GRCm38)	R7299*	probably null	Het
Odf2l	G	A	3: 145,139,820 (GRCm38)	V363I	possibly damaging	Het
Olfr721-ps1	A	G	14: 14,407,998 (GRCm38)	T257A	possibly damaging	Het
Or2g25	T	C	17: 37,659,808 (GRCm38)	H175R	probably damaging	Het
Or4c100	A	T	2: 88,525,826 (GRCm38)	D81V	probably damaging	Het
Or52a5b	A	T	7: 103,767,733 (GRCm38)	Y221*	probably null	Het
Or5b105	T	A	19: 13,103,173 (GRCm38)	I44F	probably benign	Het
Otud3	G	A	4: 138,896,756 (GRCm38)	P325L	probably benign	Het
Palb2	T	C	7: 122,124,834 (GRCm38)	N564S	probably benign	Het
Pcnt	A	G	10: 76,427,927 (GRCm38)	V401A	possibly damaging	Het
Plcd4	G	A	1: 74,565,365 (GRCm38)	E767K	probably benign	Het
Ppp3ca	T	C	3: 136,868,626 (GRCm38)	F95S	probably damaging	Het
Prkar2a	A	G	9: 108,692,622 (GRCm38)	T56A	probably benign	Het
Psma2	T	A	13: 14,625,230 (GRCm38)	D186E	probably benign	Het
Psmd11	T	A	11: 80,438,273 (GRCm38)	Y72*	probably null	Het
Ror1	G	A	4: 100,302,945 (GRCm38)	D53N	probably benign	Het
Rubcn	C	T	16: 32,836,469 (GRCm38)	R527Q	probably damaging	Het
Sgca	G	A	11: 94,969,547 (GRCm38)	P255S	possibly damaging	Het
Skint11	A	T	4: 114,227,796 (GRCm38)	R167S	probably benign	Het
Slco5a1	A	G	1: 12,990,437 (GRCm38)	V20A	probably benign	Het
Snai2	T	C	16: 14,707,106 (GRCm38)	S159P	probably benign	Het
Taar2	T	G	10: 23,940,827 (GRCm38)	S88R	probably damaging	Het
Tacc3	T	A	5: 33,667,165 (GRCm38)	N378K	possibly damaging	Het
Tek	A	G	4: 94,811,410 (GRCm38)	K342E	probably benign	Het
Tm9sf3	G	T	19: 41,245,505 (GRCm38)	S198*	probably null	Het
Tmbim4	T	A	10: 120,215,609 (GRCm38)	F56I	possibly damaging	Het
Tsen34	T	A	7: 3,694,987 (GRCm38)	S85T	probably benign	Het
Ttc27	C	A	17: 74,747,715 (GRCm38)	Q339K	probably benign	Het
Ubap2	G	T	4: 41,205,550 (GRCm38)	P636T	probably benign	Het
Ubr1	G	A	2: 120,875,498 (GRCm38)	L1495F	probably benign	Het
Vsig10	T	C	5: 117,343,902 (GRCm38)	S386P	probably damaging	Het
Wnk2	C	A	13: 49,147,177 (GRCm38)	R19L	probably benign	Het
Wsb2	T	C	5: 117,370,879 (GRCm38)	L126P	probably damaging	Het
Xylb	A	G	9: 119,382,292 (GRCm38)	I402V	probably benign	Het
Yrdc	A	G	4: 124,850,955 (GRCm38)	S61G	probably benign	Het
Zbtb3	A	G	19: 8,803,407 (GRCm38)	D128G	probably damaging	Het
Zfp423	C	T	8: 87,780,861 (GRCm38)	G952R	probably damaging	Het
Zfp646	A	G	7: 127,879,772 (GRCm38)	T374A	possibly damaging	Het

Other mutations in Lama3

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00091:Lama3	APN	18	12,580,292 (GRCm38)	missense	probably benign
IGL00272:Lama3	APN	18	12,491,548 (GRCm38)	missense	probably damaging	1.00
IGL00335:Lama3	APN	18	12,449,588 (GRCm38)	splice site	probably benign
IGL00836:Lama3	APN	18	12,472,228 (GRCm38)	missense	probably benign	0.01
IGL01017:Lama3	APN	18	12,441,143 (GRCm38)	critical splice donor site	probably null
IGL01025:Lama3	APN	18	12,481,037 (GRCm38)	missense	probably benign	0.09
IGL01394:Lama3	APN	18	12,531,926 (GRCm38)	missense	probably null	0.39
IGL01545:Lama3	APN	18	12,441,131 (GRCm38)	missense	probably benign	0.01
IGL01685:Lama3	APN	18	12,453,880 (GRCm38)	splice site	probably benign
IGL01863:Lama3	APN	18	12,419,936 (GRCm38)	splice site	probably benign
IGL01869:Lama3	APN	18	12,524,763 (GRCm38)	missense	possibly damaging	0.94
IGL01894:Lama3	APN	18	12,572,064 (GRCm38)	missense	probably benign	0.09
IGL02027:Lama3	APN	18	12,516,513 (GRCm38)	missense	probably damaging	1.00
IGL02106:Lama3	APN	18	12,468,314 (GRCm38)	missense	probably damaging	0.98
IGL02307:Lama3	APN	18	12,581,783 (GRCm38)	missense	probably benign	0.09
IGL02342:Lama3	APN	18	12,491,476 (GRCm38)	missense	probably damaging	1.00
IGL02377:Lama3	APN	18	12,556,750 (GRCm38)	missense	possibly damaging	0.49
IGL02401:Lama3	APN	18	12,557,727 (GRCm38)	missense	probably benign	0.02
IGL02517:Lama3	APN	18	12,537,858 (GRCm38)	critical splice donor site	probably null
IGL02644:Lama3	APN	18	12,525,853 (GRCm38)	missense	probably benign	0.12
IGL02733:Lama3	APN	18	12,578,127 (GRCm38)	missense	probably damaging	0.99
IGL02932:Lama3	APN	18	12,528,801 (GRCm38)	missense	probably damaging	1.00
IGL03006:Lama3	APN	18	12,468,368 (GRCm38)	splice site	probably benign
IGL03038:Lama3	APN	18	12,419,250 (GRCm38)	missense	probably damaging	0.99
IGL03064:Lama3	APN	18	12,439,349 (GRCm38)	missense	possibly damaging	0.72
IGL03146:Lama3	APN	18	12,527,624 (GRCm38)	missense	possibly damaging	0.66
IGL03233:Lama3	APN	18	12,481,038 (GRCm38)	missense	probably damaging	1.00
IGL03255:Lama3	APN	18	12,539,703 (GRCm38)	missense	probably damaging	1.00
IGL03369:Lama3	APN	18	12,553,283 (GRCm38)	missense	probably benign	0.05
IGL03412:Lama3	APN	18	12,419,182 (GRCm38)	missense	probably damaging	0.99
IGL02980:Lama3	UTSW	18	12,553,231 (GRCm38)	missense	probably benign	0.01
IGL03014:Lama3	UTSW	18	12,539,967 (GRCm38)	missense	possibly damaging	0.95
R0007:Lama3	UTSW	18	12,497,881 (GRCm38)	splice site	probably benign
R0007:Lama3	UTSW	18	12,497,881 (GRCm38)	splice site	probably benign
R0050:Lama3	UTSW	18	12,404,103 (GRCm38)	missense	probably damaging	1.00
R0050:Lama3	UTSW	18	12,404,103 (GRCm38)	missense	probably damaging	1.00
R0063:Lama3	UTSW	18	12,528,705 (GRCm38)	splice site	probably benign
R0063:Lama3	UTSW	18	12,528,705 (GRCm38)	splice site	probably benign
R0106:Lama3	UTSW	18	12,403,982 (GRCm38)	missense	probably damaging	0.96
R0148:Lama3	UTSW	18	12,448,272 (GRCm38)	missense	probably damaging	1.00
R0165:Lama3	UTSW	18	12,524,810 (GRCm38)	missense	probably damaging	0.99
R0240:Lama3	UTSW	18	12,539,823 (GRCm38)	splice site	probably null
R0240:Lama3	UTSW	18	12,539,823 (GRCm38)	splice site	probably null
R0316:Lama3	UTSW	18	12,519,877 (GRCm38)	missense	probably benign	0.09
R0325:Lama3	UTSW	18	12,482,126 (GRCm38)	missense	probably damaging	1.00
R0365:Lama3	UTSW	18	12,507,007 (GRCm38)	missense	probably damaging	0.96
R0390:Lama3	UTSW	18	12,407,563 (GRCm38)	missense	probably benign	0.10
R0408:Lama3	UTSW	18	12,456,837 (GRCm38)	missense	probably benign
R0449:Lama3	UTSW	18	12,500,512 (GRCm38)	splice site	probably null
R0453:Lama3	UTSW	18	12,465,478 (GRCm38)	missense	possibly damaging	0.63
R0480:Lama3	UTSW	18	12,450,424 (GRCm38)	missense	possibly damaging	0.81
R0536:Lama3	UTSW	18	12,525,894 (GRCm38)	missense	probably damaging	1.00
R0545:Lama3	UTSW	18	12,561,701 (GRCm38)	missense	possibly damaging	0.90
R0567:Lama3	UTSW	18	12,549,252 (GRCm38)	missense	probably benign
R0605:Lama3	UTSW	18	12,506,949 (GRCm38)	missense	probably benign	0.02
R0617:Lama3	UTSW	18	12,419,258 (GRCm38)	critical splice donor site	probably null
R0629:Lama3	UTSW	18	12,419,245 (GRCm38)	missense	possibly damaging	0.79
R0671:Lama3	UTSW	18	12,477,590 (GRCm38)	missense	possibly damaging	0.80
R0730:Lama3	UTSW	18	12,456,850 (GRCm38)	splice site	probably benign
R1216:Lama3	UTSW	18	12,421,134 (GRCm38)	splice site	probably benign
R1356:Lama3	UTSW	18	12,500,577 (GRCm38)	unclassified	probably benign
R1386:Lama3	UTSW	18	12,477,370 (GRCm38)	missense	probably benign	0.04
R1424:Lama3	UTSW	18	12,519,991 (GRCm38)	missense	probably benign	0.13
R1426:Lama3	UTSW	18	12,481,098 (GRCm38)	critical splice donor site	probably null
R1437:Lama3	UTSW	18	12,549,227 (GRCm38)	missense	possibly damaging	0.46
R1468:Lama3	UTSW	18	12,441,107 (GRCm38)	missense	probably benign	0.00
R1468:Lama3	UTSW	18	12,441,107 (GRCm38)	missense	probably benign	0.00
R1472:Lama3	UTSW	18	12,482,045 (GRCm38)	missense	probably benign	0.23
R1557:Lama3	UTSW	18	12,513,731 (GRCm38)	splice site	probably benign
R1571:Lama3	UTSW	18	12,539,717 (GRCm38)	missense	probably damaging	0.98
R1599:Lama3	UTSW	18	12,450,400 (GRCm38)	nonsense	probably null
R1631:Lama3	UTSW	18	12,407,494 (GRCm38)	missense	probably damaging	1.00
R1647:Lama3	UTSW	18	12,532,199 (GRCm38)	missense	possibly damaging	0.90
R1648:Lama3	UTSW	18	12,532,199 (GRCm38)	missense	possibly damaging	0.90
R1719:Lama3	UTSW	18	12,479,872 (GRCm38)	critical splice donor site	probably null
R1757:Lama3	UTSW	18	12,465,499 (GRCm38)	missense	probably benign	0.10
R1766:Lama3	UTSW	18	12,402,062 (GRCm38)	missense	probably damaging	1.00
R1853:Lama3	UTSW	18	12,513,705 (GRCm38)	missense	possibly damaging	0.75
R1856:Lama3	UTSW	18	12,537,781 (GRCm38)	nonsense	probably null
R1909:Lama3	UTSW	18	12,581,798 (GRCm38)	missense	probably benign	0.19
R1913:Lama3	UTSW	18	12,495,279 (GRCm38)	missense	probably benign	0.15
R1975:Lama3	UTSW	18	12,453,863 (GRCm38)	missense	probably damaging	1.00
R2014:Lama3	UTSW	18	12,524,721 (GRCm38)	splice site	probably benign
R2059:Lama3	UTSW	18	12,528,333 (GRCm38)	missense	probably damaging	0.98
R2060:Lama3	UTSW	18	12,528,726 (GRCm38)	missense	probably benign	0.30
R2086:Lama3	UTSW	18	12,524,830 (GRCm38)	missense	probably benign	0.39
R2115:Lama3	UTSW	18	12,402,849 (GRCm38)	missense	possibly damaging	0.94
R2291:Lama3	UTSW	18	12,525,079 (GRCm38)	missense	probably damaging	0.98
R2860:Lama3	UTSW	18	12,453,750 (GRCm38)	missense	probably damaging	1.00
R2861:Lama3	UTSW	18	12,453,750 (GRCm38)	missense	probably damaging	1.00
R2862:Lama3	UTSW	18	12,453,750 (GRCm38)	missense	probably damaging	1.00
R3410:Lama3	UTSW	18	12,413,858 (GRCm38)	critical splice donor site	probably null
R3614:Lama3	UTSW	18	12,448,288 (GRCm38)	missense	probably benign	0.03
R3696:Lama3	UTSW	18	12,439,475 (GRCm38)	splice site	probably benign
R3752:Lama3	UTSW	18	12,507,029 (GRCm38)	missense	probably damaging	1.00
R3967:Lama3	UTSW	18	12,580,341 (GRCm38)	missense	probably damaging	1.00
R3968:Lama3	UTSW	18	12,580,341 (GRCm38)	missense	probably damaging	1.00
R3969:Lama3	UTSW	18	12,580,341 (GRCm38)	missense	probably damaging	1.00
R3970:Lama3	UTSW	18	12,580,341 (GRCm38)	missense	probably damaging	1.00
R4088:Lama3	UTSW	18	12,504,308 (GRCm38)	nonsense	probably null
R4118:Lama3	UTSW	18	12,450,431 (GRCm38)	missense	probably benign	0.01
R4222:Lama3	UTSW	18	12,450,403 (GRCm38)	missense	probably damaging	1.00
R4223:Lama3	UTSW	18	12,450,403 (GRCm38)	missense	probably damaging	1.00
R4224:Lama3	UTSW	18	12,450,403 (GRCm38)	missense	probably damaging	1.00
R4225:Lama3	UTSW	18	12,450,403 (GRCm38)	missense	probably damaging	1.00
R4367:Lama3	UTSW	18	12,513,690 (GRCm38)	missense	probably damaging	1.00
R4404:Lama3	UTSW	18	12,582,531 (GRCm38)	missense	probably benign	0.01
R4424:Lama3	UTSW	18	12,519,872 (GRCm38)	nonsense	probably null
R4483:Lama3	UTSW	18	12,549,253 (GRCm38)	missense	probably benign	0.32
R4484:Lama3	UTSW	18	12,481,088 (GRCm38)	missense	probably benign
R4516:Lama3	UTSW	18	12,495,358 (GRCm38)	missense	probably damaging	1.00
R4556:Lama3	UTSW	18	12,479,759 (GRCm38)	missense	possibly damaging	0.63
R4616:Lama3	UTSW	18	12,504,397 (GRCm38)	critical splice donor site	probably null
R4702:Lama3	UTSW	18	12,578,029 (GRCm38)	nonsense	probably null
R4704:Lama3	UTSW	18	12,553,223 (GRCm38)	missense	probably benign	0.08
R4750:Lama3	UTSW	18	12,504,359 (GRCm38)	missense	probably benign	0.25
R4753:Lama3	UTSW	18	12,482,084 (GRCm38)	missense	probably damaging	1.00
R4767:Lama3	UTSW	18	12,500,563 (GRCm38)	missense	probably benign	0.32
R4777:Lama3	UTSW	18	12,413,771 (GRCm38)	missense	probably damaging	1.00
R4782:Lama3	UTSW	18	12,411,570 (GRCm38)	nonsense	probably null
R4784:Lama3	UTSW	18	12,449,544 (GRCm38)	missense	probably benign	0.20
R4816:Lama3	UTSW	18	12,477,604 (GRCm38)	missense	possibly damaging	0.93
R4833:Lama3	UTSW	18	12,441,131 (GRCm38)	missense	probably benign	0.01
R4854:Lama3	UTSW	18	12,411,542 (GRCm38)	missense	probably benign	0.00
R4863:Lama3	UTSW	18	12,498,678 (GRCm38)	intron	probably benign
R4863:Lama3	UTSW	18	12,539,793 (GRCm38)	missense	probably damaging	0.99
R4953:Lama3	UTSW	18	12,448,305 (GRCm38)	missense	probably damaging	1.00
R4974:Lama3	UTSW	18	12,552,826 (GRCm38)	missense	probably damaging	0.98
R4996:Lama3	UTSW	18	12,518,743 (GRCm38)	missense	probably benign	0.24
R5049:Lama3	UTSW	18	12,582,611 (GRCm38)	missense	probably benign	0.19
R5057:Lama3	UTSW	18	12,531,948 (GRCm38)	missense	probably null	0.82
R5090:Lama3	UTSW	18	12,542,402 (GRCm38)	missense	possibly damaging	0.94
R5122:Lama3	UTSW	18	12,539,766 (GRCm38)	missense	possibly damaging	0.53
R5215:Lama3	UTSW	18	12,577,900 (GRCm38)	missense	probably damaging	1.00
R5245:Lama3	UTSW	18	12,419,893 (GRCm38)	missense	probably damaging	1.00
R5259:Lama3	UTSW	18	12,465,508 (GRCm38)	missense	probably damaging	1.00
R5320:Lama3	UTSW	18	12,552,855 (GRCm38)	missense	probably damaging	0.99
R5377:Lama3	UTSW	18	12,453,746 (GRCm38)	missense	probably damaging	0.99
R5432:Lama3	UTSW	18	12,572,066 (GRCm38)	missense	probably damaging	1.00
R5500:Lama3	UTSW	18	12,456,764 (GRCm38)	missense	possibly damaging	0.93
R5534:Lama3	UTSW	18	12,553,210 (GRCm38)	missense	probably benign	0.00
R5589:Lama3	UTSW	18	12,472,220 (GRCm38)	missense	possibly damaging	0.46
R5604:Lama3	UTSW	18	12,439,348 (GRCm38)	missense	probably benign
R5617:Lama3	UTSW	18	12,498,936 (GRCm38)	intron	probably benign
R5709:Lama3	UTSW	18	12,539,799 (GRCm38)	missense	probably damaging	1.00
R5965:Lama3	UTSW	18	12,429,887 (GRCm38)	missense	possibly damaging	0.67
R6042:Lama3	UTSW	18	12,574,254 (GRCm38)	missense	probably damaging	1.00
R6065:Lama3	UTSW	18	12,469,928 (GRCm38)	missense	possibly damaging	0.53
R6085:Lama3	UTSW	18	12,482,099 (GRCm38)	missense	probably benign	0.01
R6212:Lama3	UTSW	18	12,513,645 (GRCm38)	missense	probably damaging	1.00
R6268:Lama3	UTSW	18	12,524,737 (GRCm38)	missense	probably damaging	0.98
R6276:Lama3	UTSW	18	12,506,949 (GRCm38)	missense	probably benign	0.02
R6366:Lama3	UTSW	18	12,482,137 (GRCm38)	missense	probably damaging	1.00
R6393:Lama3	UTSW	18	12,479,756 (GRCm38)	missense	probably benign	0.44
R6493:Lama3	UTSW	18	12,482,148 (GRCm38)	critical splice donor site	probably null
R6505:Lama3	UTSW	18	12,495,348 (GRCm38)	missense	probably benign	0.02
R6563:Lama3	UTSW	18	12,537,766 (GRCm38)	missense	probably damaging	1.00
R6582:Lama3	UTSW	18	12,577,840 (GRCm38)	missense	probably damaging	1.00
R6585:Lama3	UTSW	18	12,419,257 (GRCm38)	critical splice donor site	probably null
R6609:Lama3	UTSW	18	12,513,678 (GRCm38)	missense	probably damaging	0.99
R6656:Lama3	UTSW	18	12,549,226 (GRCm38)	missense	possibly damaging	0.66
R6833:Lama3	UTSW	18	12,491,548 (GRCm38)	missense	probably damaging	1.00
R6834:Lama3	UTSW	18	12,491,548 (GRCm38)	missense	probably damaging	1.00
R7019:Lama3	UTSW	18	12,528,418 (GRCm38)	missense	probably damaging	0.97
R7026:Lama3	UTSW	18	12,516,548 (GRCm38)	missense	probably damaging	0.98
R7088:Lama3	UTSW	18	12,582,545 (GRCm38)	missense	possibly damaging	0.90
R7100:Lama3	UTSW	18	12,582,644 (GRCm38)	missense	possibly damaging	0.80
R7102:Lama3	UTSW	18	12,552,813 (GRCm38)	missense	possibly damaging	0.66
R7103:Lama3	UTSW	18	12,531,879 (GRCm38)	missense	probably benign	0.00
R7133:Lama3	UTSW	18	12,539,786 (GRCm38)	missense	probably benign	0.05
R7150:Lama3	UTSW	18	12,468,289 (GRCm38)	missense	probably damaging	1.00
R7158:Lama3	UTSW	18	12,456,812 (GRCm38)	missense	probably benign	0.20
R7170:Lama3	UTSW	18	12,404,076 (GRCm38)	missense	probably benign	0.26
R7216:Lama3	UTSW	18	12,430,000 (GRCm38)	missense	probably damaging	1.00
R7223:Lama3	UTSW	18	12,582,608 (GRCm38)	missense	possibly damaging	0.53
R7243:Lama3	UTSW	18	12,419,845 (GRCm38)	missense	probably damaging	1.00
R7282:Lama3	UTSW	18	12,439,392 (GRCm38)	missense	probably damaging	0.99
R7337:Lama3	UTSW	18	12,507,040 (GRCm38)	splice site	probably null
R7442:Lama3	UTSW	18	12,472,181 (GRCm38)	critical splice acceptor site	probably null
R7487:Lama3	UTSW	18	12,419,237 (GRCm38)	missense	probably benign
R7604:Lama3	UTSW	18	12,500,493 (GRCm38)	missense	possibly damaging	0.93
R7609:Lama3	UTSW	18	12,531,834 (GRCm38)	critical splice acceptor site	probably null
R7650:Lama3	UTSW	18	12,537,838 (GRCm38)	missense	probably benign	0.01
R7894:Lama3	UTSW	18	12,462,807 (GRCm38)	missense	probably benign	0.07
R7975:Lama3	UTSW	18	12,537,739 (GRCm38)	missense	probably damaging	1.00
R8099:Lama3	UTSW	18	12,534,063 (GRCm38)	missense	probably damaging	0.97
R8168:Lama3	UTSW	18	12,506,942 (GRCm38)	missense	probably null
R8219:Lama3	UTSW	18	12,439,360 (GRCm38)	missense	probably benign	0.07
R8227:Lama3	UTSW	18	12,407,551 (GRCm38)	missense	probably benign
R8229:Lama3	UTSW	18	12,407,551 (GRCm38)	missense	probably benign
R8298:Lama3	UTSW	18	12,525,853 (GRCm38)	missense	probably benign	0.12
R8351:Lama3	UTSW	18	12,540,613 (GRCm38)	missense	probably damaging	1.00
R8364:Lama3	UTSW	18	12,528,347 (GRCm38)	missense	probably damaging	0.99
R8463:Lama3	UTSW	18	12,449,839 (GRCm38)	missense	probably damaging	0.96
R8515:Lama3	UTSW	18	12,411,631 (GRCm38)	missense	probably null	0.01
R8784:Lama3	UTSW	18	12,421,155 (GRCm38)	missense	probably benign
R8799:Lama3	UTSW	18	12,490,943 (GRCm38)	missense	probably damaging	0.96
R8874:Lama3	UTSW	18	12,449,586 (GRCm38)	critical splice donor site	probably null
R8938:Lama3	UTSW	18	12,556,705 (GRCm38)	missense	probably damaging	1.00
R8967:Lama3	UTSW	18	12,532,039 (GRCm38)	missense	possibly damaging	0.46
R9039:Lama3	UTSW	18	12,481,063 (GRCm38)	nonsense	probably null
R9126:Lama3	UTSW	18	12,450,470 (GRCm38)	missense	probably damaging	1.00
R9200:Lama3	UTSW	18	12,472,240 (GRCm38)	missense	probably benign	0.00
R9203:Lama3	UTSW	18	12,462,812 (GRCm38)	missense	probably benign	0.04
R9246:Lama3	UTSW	18	12,577,902 (GRCm38)	missense	probably damaging	0.99
R9284:Lama3	UTSW	18	12,450,484 (GRCm38)	nonsense	probably null
R9553:Lama3	UTSW	18	12,429,962 (GRCm38)	missense	probably damaging	1.00
R9716:Lama3	UTSW	18	12,450,403 (GRCm38)	missense	probably damaging	1.00
R9734:Lama3	UTSW	18	12,549,263 (GRCm38)	missense	possibly damaging	0.94
X0019:Lama3	UTSW	18	12,582,574 (GRCm38)	missense	possibly damaging	0.94
Z1177:Lama3	UTSW	18	12,429,879 (GRCm38)	critical splice acceptor site	probably null

Predicted Primers

PCR Primer
(F):5'- TGCTCAATGCCTGTGATGTCC -3'
(R):5'- CCCCTGAGAAACACTTCTGG -3'

Sequencing Primer
(F):5'- CCTAAGACTTTGTTGAAAGGACGTC -3'
(R):5'- CTGAGAAACACTTCTGGCTCTTGAG -3'

Posted On

2019-05-15