Mutagenetix > Incidental Mutations

Incidental Mutation 'R7122:Olfr340'

552051

Institutional Source

Beutler Lab

Gene Symbol

Olfr340

Ensembl Gene

ENSMUSG00000094266

Gene Name

olfactory receptor 340

Synonyms

GA_x6K02T2NLDC-33147742-33148680, MOR136-1

MMRRC Submission

Accession Numbers

Is this an essential gene?

Probably non essential (E-score: 0.091) question?

Stock #

R7122 (G1)

Quality Score

225.009

Status

Validated

Chromosome

Chromosomal Location

36452587-36453525 bp(+) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

A to G at 36452690 bp

Zygosity

Heterozygous

Amino Acid Change

Tyrosine to Cysteine at position 35 (Y35C)

Ref Sequence

ENSEMBL: ENSMUSP00000072632 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000072854]

Predicted Effect

probably damaging
Transcript: ENSMUST00000072854
AA Change: Y35C

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)

SMART Domains

Protein: ENSMUSP00000072632
Gene: ENSMUSG00000094266
AA Change: Y35C

Domain	Start	End	E-Value	Type
Pfam:7tm_4	31	308	4.2e-56	PFAM
Pfam:7TM_GPCR_Srsx	35	305	5.8e-6	PFAM
Pfam:7tm_1	41	290	1.4e-23	PFAM

Meta Mutation Damage Score

0.1144

Coding Region Coverage

1x: 100.0%
3x: 100.0%
10x: 99.7%
20x: 99.0%

Validation Efficiency

99% (75/76)

MGI Phenotype

FUNCTION: Olfactory receptors interact with odorant molecules in the nose, to initiate a neuronal response that triggers the perception of a smell. The olfactory receptor proteins are members of a large family of G-protein-coupled receptors (GPCR) arising from single coding-exon genes. Olfactory receptors share a 7-transmembrane domain structure with many neurotransmitter and hormone receptors and are responsible for the recognition and G protein-mediated transduction of odorant signals. The olfactory receptor gene family is the largest in the genome. The nomenclature assigned to the olfactory receptor genes and proteins for this organism is independent of other organisms. [provided by RefSeq, Jul 2008]

Allele List at MGI

Other mutations in this stock

Total: 74 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Akr1c20	T	A	13: 4,511,276	E126V	probably benign	Het
Alg3	A	T	16: 20,607,852	L213H	probably damaging	Het
Atp1a4	T	A	1: 172,231,936	Y863F	possibly damaging	Het
Atp6v0e	A	G	17: 26,695,416	T72A	probably benign	Het
Aup1	A	G	6: 83,055,142	T97A	probably benign	Het
Bcl6	T	C	16: 23,972,902	D234G	probably damaging	Het
Cdc42bpa	T	C	1: 180,065,018	L249P	probably damaging	Het
Celsr3	A	G	9: 108,828,567	K750E	possibly damaging	Het
Ces1g	C	A	8: 93,317,037	G425C	possibly damaging	Het
Chl1	T	C	6: 103,706,448	L745P	probably damaging	Het
Csmd2	G	A	4: 128,449,227	V1471M		Het
Dnah12	G	A	14: 26,778,912		probably null	Het
E330017A01Rik	C	A	16: 58,637,777	A54S	probably benign	Het
E4f1	G	A	17: 24,444,834	Q569*	probably null	Het
Fbxw24	A	T	9: 109,601,260	C439S	probably benign	Het
Gm11639	A	T	11: 105,008,961	I4350F	possibly damaging	Het
Gmip	C	T	8: 69,817,802	P721S	probably benign	Het
Grip1	A	G	10: 120,035,374	I669V	possibly damaging	Het
Gsn	A	T	2: 35,295,049	K339*	probably null	Het
Herc1	G	T	9: 66,399,774	A959S	possibly damaging	Het
Hic1	A	G	11: 75,169,230	V97A	probably benign	Het
Il11ra1	A	G	4: 41,766,189	Y242C	probably damaging	Het
Iqsec3	A	C	6: 121,473,358	V69G	unknown	Het
Jak3	T	A	8: 71,685,957	M933K	probably damaging	Het
Kbtbd7	G	A	14: 79,428,317	V530I	probably damaging	Het
Kif22	G	A	7: 127,032,978	R345C	probably benign	Het
Klhl7	G	A	5: 24,138,435	E250K	probably damaging	Het
Klk1b4	A	G	7: 44,211,107	H183R	probably damaging	Het
Lhfpl4	C	T	6: 113,176,671	V140I	probably benign	Het
Lhx5	T	A	5: 120,436,345	M238K	probably benign	Het
Lin28b	A	T	10: 45,469,148	H27Q	probably benign	Het
Mgat4c	A	T	10: 102,378,209	R18*	probably null	Het
Myct1	C	A	10: 5,604,492	H120N	probably damaging	Het
Nek1	A	T	8: 61,106,795	D984V	probably benign	Het
Nfu1	A	T	6: 87,009,881		probably benign	Het
Nipsnap1	A	T	11: 4,883,366		probably null	Het
Nlrp9c	A	G	7: 26,385,621	Y178H	probably damaging	Het
Nrxn3	T	A	12: 89,510,607	M520K	probably damaging	Het
Oacyl	A	G	18: 65,720,252	D143G	probably benign	Het
Obox5	A	G	7: 15,758,807	Y229C	probably damaging	Het
Olfr347	G	A	2: 36,734,424	M34I	probably benign	Het
Olfr448	T	C	6: 42,897,090	V213A	probably damaging	Het
Olfr669	T	C	7: 104,939,198	L224P	probably damaging	Het
Otogl	C	T	10: 107,866,654	A713T	probably benign	Het
Pcdhb17	A	C	18: 37,486,513	N452T	probably benign	Het
Pi16	A	G	17: 29,326,339	Y192C	probably damaging	Het
Pla2g5	T	C	4: 138,804,519	D58G	probably damaging	Het
Plpp4	G	T	7: 129,379,483	V153F	unknown	Het
Plxna2	C	A	1: 194,644,568	S270*	probably null	Het
Pole	T	A	5: 110,325,102		probably null	Het
Prmt7	T	C	8: 106,235,100	F215S	unknown	Het
Ptpn21	C	T	12: 98,688,912	V599I	probably damaging	Het
Rab26	C	A	17: 24,530,678	R131L	probably damaging	Het
Raph1	A	T	1: 60,525,977	V117D	probably benign	Het
Sacs	T	C	14: 61,210,396	V3297A	probably damaging	Het
Serpine1	C	A	5: 137,066,942	A262S	probably benign	Het
Sgf29	A	G	7: 126,672,049	D193G	probably null	Het
Sh3rf2	G	A	18: 42,104,162		probably null	Het
Sipa1l1	T	C	12: 82,422,462	V1245A	possibly damaging	Het
Slc22a7	A	G	17: 46,438,298	L31P	probably damaging	Het
Slc24a5	G	A	2: 125,088,191	V471I	probably benign	Het
Slc26a7	A	T	4: 14,533,639	Y395N	probably damaging	Het
Slc38a2	T	C	15: 96,693,301	M229V	probably damaging	Het
Sorbs1	G	C	19: 40,376,800	R180G	probably benign	Het
Susd1	G	A	4: 59,411,318	R225*	probably null	Het
Suz12	T	A	11: 79,993,593	F92I	probably damaging	Het
Tmem114	C	A	16: 8,424,746		probably benign	Het
Tmem68	A	G	4: 3,564,107	V159A	probably benign	Het
Tpra1	T	A	6: 88,908,294	I76N	probably damaging	Het
Trim45	A	G	3: 100,932,037	T752A	unknown	Het
Trp63	C	A	16: 25,820,477	H138Q	probably damaging	Het
Ugt2a2	T	C	5: 87,460,396	D528G	possibly damaging	Het
Vmn2r39	T	G	7: 9,014,762	K858N	possibly damaging	Het
Zfp629	A	T	7: 127,611,312	S442T	probably damaging	Het

Other mutations in Olfr340

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01467:Olfr340	APN	2	36452644	nonsense	probably null
IGL01590:Olfr340	APN	2	36452992	missense	probably benign	0.39
IGL01614:Olfr340	APN	2	36452624	missense	probably benign	0.32
IGL02470:Olfr340	APN	2	36452597	missense	probably benign	0.00
IGL02943:Olfr340	APN	2	36453039	missense	probably benign	0.05
R0089:Olfr340	UTSW	2	36453095	missense	probably benign	0.00
R0600:Olfr340	UTSW	2	36452648	missense	probably benign	0.06
R0881:Olfr340	UTSW	2	36453440	missense	probably damaging	1.00
R1945:Olfr340	UTSW	2	36453031	missense	probably damaging	1.00
R2184:Olfr340	UTSW	2	36453034	missense	probably benign
R2196:Olfr340	UTSW	2	36452588	start codon destroyed	probably null	1.00
R2419:Olfr340	UTSW	2	36453326	missense	probably damaging	1.00
R2859:Olfr340	UTSW	2	36453130	missense	probably benign	0.01
R2964:Olfr340	UTSW	2	36452767	missense	probably damaging	1.00
R4677:Olfr340	UTSW	2	36453050	missense	probably benign	0.00
R4867:Olfr340	UTSW	2	36453199	missense	probably benign
R5468:Olfr340	UTSW	2	36453443	missense	probably damaging	0.99
R5582:Olfr340	UTSW	2	36453221	missense	probably benign	0.03
R6335:Olfr340	UTSW	2	36452722	missense	probably benign	0.22
R6415:Olfr340	UTSW	2	36452605	missense	probably damaging	0.99
R6664:Olfr340	UTSW	2	36453098	missense	probably benign	0.00
R6873:Olfr340	UTSW	2	36453496	missense	probably benign	0.00
R7097:Olfr340	UTSW	2	36452690	missense	probably damaging	1.00
R7199:Olfr340	UTSW	2	36452860	missense	probably damaging	1.00
R7275:Olfr340	UTSW	2	36452839	missense	probably benign	0.05
R7812:Olfr340	UTSW	2	36453278	missense	probably benign	0.00
Z1088:Olfr340	UTSW	2	36452906	missense	possibly damaging	0.66

Predicted Primers

PCR Primer
(F):5'- GATCTACAACTGGGTCTCATCTG -3'
(R):5'- ACACCCAGCATATGAGATGG -3'

Sequencing Primer
(F):5'- ACTGGGTCTCATCTGATAATAGTTC -3'
(R):5'- CCCAGCATATGAGATGGATTGACTG -3'

Posted On

2019-05-15