Mutagenetix > Incidental Mutation

Incidental Mutation 'R7131:Cfap54'

552673

Institutional Source

Beutler Lab

Gene Symbol

Cfap54

Ensembl Gene

ENSMUSG00000020014

Gene Name

cilia and flagella associated protein 54

Synonyms

LOC380653, Gm872, 4930485B16Rik

MMRRC Submission

045216-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.071) question?

Stock #

R7131 (G1)

Quality Score

225.009

Status

Not validated

Chromosome

Chromosomal Location

92775619-93081618 bp(-) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

T to C at 92821104 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Lysine to Glutamic Acid at position 3029 (K3029E)

Ref Sequence

ENSEMBL: ENSMUSP00000148636 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000168110] [ENSMUST00000212902]

AlphaFold

no structure available at present

Predicted Effect

unknown
Transcript: ENSMUST00000168110
AA Change: K2964E

SMART Domains

Protein: ENSMUSP00000129517
Gene: ENSMUSG00000020014
AA Change: K2964E

Domain	Start	End	E-Value	Type
low complexity region	3	37	N/A	INTRINSIC
low complexity region	39	48	N/A	INTRINSIC
Pfam:DUF4486	104	642	1.1e-269	PFAM
low complexity region	842	851	N/A	INTRINSIC
low complexity region	902	915	N/A	INTRINSIC
Blast:FN3	916	1002	4e-48	BLAST
low complexity region	1409	1426	N/A	INTRINSIC
low complexity region	1974	1984	N/A	INTRINSIC
low complexity region	2354	2370	N/A	INTRINSIC
low complexity region	2500	2513	N/A	INTRINSIC
low complexity region	2605	2616	N/A	INTRINSIC

Predicted Effect

probably benign
Transcript: ENSMUST00000212902
AA Change: K3029E

PolyPhen 2 Score 0.026 (Sensitivity: 0.95; Specificity: 0.81)

Coding Region Coverage

1x: 100.0%
3x: 100.0%
10x: 99.7%
20x: 99.0%

Validation Efficiency

MGI Phenotype

PHENOTYPE: Homozygous inactivation of this gene causes background-dependent lethality and hydroencephaly, male sterility associated with defects in spermiogenesis, and impaired mucociliary clearance. Airway epithelial cilia show structural defects and a decrease in ciliary beat frequency and cilia-driven flow. [provided by MGI curators]

Allele List at MGI

Other mutations in this stock

Total: 100 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Aadacl4fm4	A	G	4: 144,670,067 (GRCm38)	V365A	probably damaging	Het
Abca13	A	G	11: 9,291,893 (GRCm38)	N1252S	probably benign	Het
Abcc3	A	T	11: 94,365,031 (GRCm38)	F543I	probably damaging	Het
Adam15	T	A	3: 89,346,980 (GRCm38)	Q170L	possibly damaging	Het
Aldoc	A	G	11: 78,324,456 (GRCm38)	I19V	possibly damaging	Het
Aoc1l1	T	A	6: 48,976,372 (GRCm38)	Y410*	probably null	Het
Atrip	T	C	9: 109,060,420 (GRCm38)	I711M	probably benign	Het
Brwd1	A	C	16: 96,066,498 (GRCm38)	L149R	probably damaging	Het
Capn9	A	T	8: 124,576,278 (GRCm38)	D45V	probably damaging	Het
Card9	G	A	2: 26,358,835 (GRCm38)	R101C	probably damaging	Het
Ccdc87	A	G	19: 4,841,757 (GRCm38)	E759G	probably damaging	Het
Ccn1	T	C	3: 145,648,781 (GRCm38)	D125G	probably damaging	Het
Cep250	T	C	2: 155,965,077 (GRCm38)	M193T	probably damaging	Het
Chrna9	G	A	5: 65,977,141 (GRCm38)	G445D	possibly damaging	Het
Cluap1	C	T	16: 3,940,775 (GRCm38)	S367L	probably benign	Het
Col5a1	A	G	2: 27,929,486 (GRCm38)	D200G	unknown	Het
Crhr2	T	C	6: 55,092,127 (GRCm38)	N388D		Het
Cyp2b23	A	G	7: 26,681,413 (GRCm38)	L129P	probably benign	Het
Dctd	A	G	8: 48,112,040 (GRCm38)	S67G	probably benign	Het
Dhtkd1	C	G	2: 5,904,070 (GRCm38)	V738L	probably benign	Het
Dnah17	C	T	11: 118,079,658 (GRCm38)	D2173N	probably benign	Het
Dnah7c	G	T	1: 46,681,772 (GRCm38)	A2819S	probably benign	Het
Dnai7	A	T	6: 145,177,406 (GRCm38)	L578Q	probably null	Het
Dot1l	A	G	10: 80,792,341 (GRCm38)	H1071R	unknown	Het
Dync2h1	T	A	9: 7,075,786 (GRCm38)	D3027V	probably damaging	Het
Efl1	A	G	7: 82,658,064 (GRCm38)	Y56C	probably damaging	Het
Eif4e1b	A	T	13: 54,784,100 (GRCm38)	R29W	probably null	Het
Evc2	A	G	5: 37,410,258 (GRCm38)	R860G	probably damaging	Het
Fbxl12	G	A	9: 20,644,383 (GRCm38)		probably benign	Het
Fibp	T	A	19: 5,461,491 (GRCm38)	I129N	probably damaging	Het
Folh1	G	T	7: 86,726,112 (GRCm38)	H555Q	probably damaging	Het
Gcnt4	A	T	13: 96,946,519 (GRCm38)	T108S	probably damaging	Het
H1f7	T	A	15: 98,256,369 (GRCm38)	K300*	probably null	Het
Hecw2	A	T	1: 53,865,121 (GRCm38)	V1156E	probably damaging	Het
Herc3	C	G	6: 58,887,424 (GRCm38)	A681G	probably damaging	Het
Igkv13-84	T	A	6: 68,939,780 (GRCm38)	C20*	probably null	Het
Iho1	A	T	9: 108,417,420 (GRCm38)	D98E	probably benign	Het
Iqca1l	A	G	5: 24,548,956 (GRCm38)	V435A	possibly damaging	Het
Ivd	C	A	2: 118,869,774 (GRCm38)	T94K	probably damaging	Het
Kank2	C	T	9: 21,794,679 (GRCm38)	A348T	probably benign	Het
Kcnma1	T	C	14: 23,367,494 (GRCm38)	Y889C	probably damaging	Het
Kif13b	T	C	14: 64,773,068 (GRCm38)	V1272A	probably damaging	Het
Kmt2d	GCTGCTGCT	GCTGCTGCTCCTGCTGCT	15: 98,849,616 (GRCm38)		probably benign	Het
Kmt5b	T	A	19: 3,815,412 (GRCm38)	D825E	probably benign	Het
Krt39	C	T	11: 99,520,871 (GRCm38)	A130T	probably benign	Het
Krtap4-9	C	T	11: 99,785,457 (GRCm38)	T68I	unknown	Het
Lmbr1l	A	T	15: 98,906,323 (GRCm38)	V365E	probably benign	Het
Lonp1	C	T	17: 56,617,814 (GRCm38)	R531Q	probably damaging	Het
Mecom	T	A	3: 29,980,945 (GRCm38)	H194L	probably damaging	Het
Mlx	C	T	11: 101,089,242 (GRCm38)	H188Y	probably damaging	Het
Mrps10	T	C	17: 47,375,015 (GRCm38)	S77P	probably damaging	Het
Mst1	A	G	9: 108,084,931 (GRCm38)	E716G	probably null	Het
Mttp	C	T	3: 138,116,132 (GRCm38)	V210I	probably benign	Het
Mug2	T	C	6: 122,075,247 (GRCm38)	V988A	probably damaging	Het
Ndufb6	A	G	4: 40,279,336 (GRCm38)	M1T	probably null	Het
Neurog1	A	T	13: 56,251,750 (GRCm38)	N61K	probably benign	Het
Nlrp14	A	G	7: 107,184,814 (GRCm38)	D581G	possibly damaging	Het
Nlrp4a	C	A	7: 26,449,833 (GRCm38)	N288K	probably benign	Het
Notch3	T	A	17: 32,144,217 (GRCm38)	H1264L	probably benign	Het
Or1e25	A	T	11: 73,602,736 (GRCm38)	D52V	possibly damaging	Het
Or1e34	A	T	11: 73,887,954 (GRCm38)	C139*	probably null	Het
Or3a1c	A	G	11: 74,155,780 (GRCm38)	M209V	probably benign	Het
Patl2	A	T	2: 122,121,782 (GRCm38)		probably null	Het
Pfkfb4	A	G	9: 109,007,302 (GRCm38)	T133A	probably benign	Het
Pla2g4f	T	C	2: 120,304,554 (GRCm38)	E471G	probably null	Het
Postn	T	C	3: 54,362,635 (GRCm38)	V45A	probably damaging	Het
Ppan	T	A	9: 20,891,154 (GRCm38)	V257E	possibly damaging	Het
Ptcra	C	G	17: 46,763,596 (GRCm38)	A7P	probably damaging	Het
Ptprd	C	T	4: 76,066,340 (GRCm38)	R523H	probably damaging	Het
Pycr3	A	T	15: 75,918,695 (GRCm38)	I105N	possibly damaging	Het
Rfx1	C	A	8: 84,095,079 (GRCm38)	Q815K	probably damaging	Het
Rfx3	T	A	19: 27,768,628 (GRCm38)	K668*	probably null	Het
Ryr2	A	T	13: 11,640,327 (GRCm38)	D3661E	possibly damaging	Het
Ryr2	A	G	13: 11,668,811 (GRCm38)		probably null	Het
Sec23ip	T	A	7: 128,779,640 (GRCm38)	S974T	probably damaging	Het
Semp2l2a	A	T	8: 13,836,982 (GRCm38)	W370R	probably damaging	Het
Setbp1	A	G	18: 79,086,960 (GRCm38)	F19S	probably benign	Het
Setd1a	AAGCAGCAGCAGCAGCAGCAG	AAGCAGCAGCAGCAGCAG	7: 127,796,418 (GRCm38)		probably benign	Het
Sh3pxd2b	A	G	11: 32,422,072 (GRCm38)	K413R	probably damaging	Het
Slc39a12	T	C	2: 14,449,803 (GRCm38)	V544A	probably damaging	Het
Slc6a7	T	C	18: 61,002,202 (GRCm38)	Y418C	probably damaging	Het
Snx19	T	G	9: 30,427,893 (GRCm38)	I109S	probably damaging	Het
Spart	T	C	3: 55,121,799 (GRCm38)		probably null	Het
Sptbn2	A	T	19: 4,749,460 (GRCm38)	Q2097L	probably null	Het
Syne1	T	C	10: 5,228,221 (GRCm38)	K4751R	probably damaging	Het
Tigit	C	A	16: 43,662,252 (GRCm38)	G40C	probably damaging	Het
Tmem181a	T	A	17: 6,297,972 (GRCm38)	I264K	probably damaging	Het
Tom1	T	A	8: 75,057,249 (GRCm38)	I287N	possibly damaging	Het
Top2a	G	A	11: 99,004,182 (GRCm38)	P864L	possibly damaging	Het
Trmt44	A	T	5: 35,571,066 (GRCm38)	V290E	probably damaging	Het
Try4	T	C	6: 41,304,403 (GRCm38)	I93T	probably benign	Het
Usp24	T	A	4: 106,382,303 (GRCm38)	H1147Q	possibly damaging	Het
Uspl1	A	T	5: 149,193,935 (GRCm38)	R109S	probably benign	Het
Vav3	T	A	3: 109,664,346 (GRCm38)	F755I	probably damaging	Het
Vezt	A	T	10: 93,970,547 (GRCm38)	Y667*	probably null	Het
Vmn1r231	T	A	17: 20,889,878 (GRCm38)	L258F	possibly damaging	Het
Zfp207	T	C	11: 80,395,528 (GRCm38)	M489T	unknown	Het
Zfp462	A	G	4: 55,009,380 (GRCm38)	T449A	probably benign	Het
Zfp956	C	A	6: 47,955,847 (GRCm38)	Q19K	probably benign	Het
Zkscan8	A	T	13: 21,525,273 (GRCm38)	W152R	probably damaging	Het

Other mutations in Cfap54

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01329:Cfap54	APN	10	93,081,523 (GRCm38)	missense	unknown
IGL02034:Cfap54	APN	10	93,061,485 (GRCm38)	missense	probably damaging	0.99
IGL02082:Cfap54	APN	10	93,081,458 (GRCm38)	missense	unknown
IGL02434:Cfap54	APN	10	93,066,754 (GRCm38)	missense	probably benign	0.20
R0011:Cfap54	UTSW	10	93,065,225 (GRCm38)	missense	probably damaging	0.97
R0011:Cfap54	UTSW	10	93,065,225 (GRCm38)	missense	probably damaging	0.97
R0032:Cfap54	UTSW	10	92,932,697 (GRCm38)	missense	probably benign	0.04
R0032:Cfap54	UTSW	10	92,932,697 (GRCm38)	missense	probably benign	0.04
R0040:Cfap54	UTSW	10	92,977,039 (GRCm38)	missense	probably benign	0.33
R0044:Cfap54	UTSW	10	93,035,433 (GRCm38)	missense	probably null	0.46
R0086:Cfap54	UTSW	10	93,028,594 (GRCm38)	missense	possibly damaging	0.86
R0104:Cfap54	UTSW	10	93,028,652 (GRCm38)	missense	probably damaging	1.00
R0194:Cfap54	UTSW	10	93,034,662 (GRCm38)	unclassified	probably benign
R0234:Cfap54	UTSW	10	92,899,160 (GRCm38)	nonsense	probably null
R0308:Cfap54	UTSW	10	92,885,364 (GRCm38)	missense	unknown
R0332:Cfap54	UTSW	10	93,035,457 (GRCm38)	missense	probably damaging	1.00
R0409:Cfap54	UTSW	10	92,776,213 (GRCm38)	missense	probably benign	0.00
R0433:Cfap54	UTSW	10	92,979,080 (GRCm38)	splice site	probably benign
R0436:Cfap54	UTSW	10	93,038,975 (GRCm38)	missense	possibly damaging	0.95
R0463:Cfap54	UTSW	10	92,874,943 (GRCm38)	critical splice donor site	probably null
R0523:Cfap54	UTSW	10	92,908,883 (GRCm38)	utr 3 prime	probably benign
R0551:Cfap54	UTSW	10	93,025,122 (GRCm38)	missense	probably benign	0.35
R0595:Cfap54	UTSW	10	92,884,736 (GRCm38)	missense	unknown
R0617:Cfap54	UTSW	10	92,829,650 (GRCm38)	splice site	probably benign
R0632:Cfap54	UTSW	10	92,885,096 (GRCm38)	missense	unknown
R0730:Cfap54	UTSW	10	93,034,737 (GRCm38)	missense	probably benign	0.05
R0786:Cfap54	UTSW	10	92,967,535 (GRCm38)	missense	possibly damaging	0.72
R0883:Cfap54	UTSW	10	92,870,669 (GRCm38)	missense	unknown
R1004:Cfap54	UTSW	10	93,066,696 (GRCm38)	splice site	probably benign
R1033:Cfap54	UTSW	10	92,839,449 (GRCm38)	missense	probably benign	0.07
R1168:Cfap54	UTSW	10	92,937,920 (GRCm38)	missense	probably damaging	0.99
R1186:Cfap54	UTSW	10	92,875,994 (GRCm38)	missense	unknown
R1429:Cfap54	UTSW	10	92,821,038 (GRCm38)	missense	probably benign	0.01
R1443:Cfap54	UTSW	10	92,932,721 (GRCm38)	missense	probably damaging	1.00
R1467:Cfap54	UTSW	10	92,969,763 (GRCm38)	missense	probably benign	0.01
R1557:Cfap54	UTSW	10	92,984,227 (GRCm38)	missense	possibly damaging	0.68
R1687:Cfap54	UTSW	10	92,932,640 (GRCm38)	missense	probably damaging	1.00
R1690:Cfap54	UTSW	10	93,035,442 (GRCm38)	missense	possibly damaging	0.95
R1711:Cfap54	UTSW	10	93,011,020 (GRCm38)	missense	probably damaging	1.00
R1756:Cfap54	UTSW	10	93,048,061 (GRCm38)	missense	probably damaging	1.00
R1769:Cfap54	UTSW	10	92,904,263 (GRCm38)	critical splice donor site	probably null
R1835:Cfap54	UTSW	10	92,962,375 (GRCm38)	missense	probably benign	0.35
R1889:Cfap54	UTSW	10	93,034,710 (GRCm38)	missense	possibly damaging	0.94
R1915:Cfap54	UTSW	10	92,884,702 (GRCm38)	missense	unknown
R1958:Cfap54	UTSW	10	92,997,342 (GRCm38)	missense	probably benign	0.18
R2005:Cfap54	UTSW	10	92,884,768 (GRCm38)	missense	unknown
R2018:Cfap54	UTSW	10	93,016,604 (GRCm38)	missense	probably benign	0.00
R2045:Cfap54	UTSW	10	93,038,809 (GRCm38)	splice site	probably null
R2059:Cfap54	UTSW	10	92,942,979 (GRCm38)	unclassified	probably benign
R2100:Cfap54	UTSW	10	93,001,937 (GRCm38)	missense	possibly damaging	0.84
R2110:Cfap54	UTSW	10	92,886,367 (GRCm38)	missense	unknown
R2392:Cfap54	UTSW	10	93,025,011 (GRCm38)	critical splice donor site	probably null
R2508:Cfap54	UTSW	10	92,997,374 (GRCm38)	missense	possibly damaging	0.72
R2852:Cfap54	UTSW	10	92,940,155 (GRCm38)	missense	probably damaging	1.00
R2857:Cfap54	UTSW	10	93,045,282 (GRCm38)	missense	probably damaging	0.99
R2871:Cfap54	UTSW	10	92,921,419 (GRCm38)	missense	possibly damaging	0.86
R2871:Cfap54	UTSW	10	92,921,419 (GRCm38)	missense	possibly damaging	0.86
R3107:Cfap54	UTSW	10	92,994,683 (GRCm38)	missense	probably benign	0.04
R3108:Cfap54	UTSW	10	92,994,683 (GRCm38)	missense	probably benign	0.04
R3157:Cfap54	UTSW	10	92,999,056 (GRCm38)	missense	probably benign	0.03
R3158:Cfap54	UTSW	10	92,999,056 (GRCm38)	missense	probably benign	0.03
R3159:Cfap54	UTSW	10	92,999,056 (GRCm38)	missense	probably benign	0.03
R3161:Cfap54	UTSW	10	93,045,278 (GRCm38)	missense	probably damaging	1.00
R3162:Cfap54	UTSW	10	93,045,278 (GRCm38)	missense	probably damaging	1.00
R3162:Cfap54	UTSW	10	93,045,278 (GRCm38)	missense	probably damaging	1.00
R3508:Cfap54	UTSW	10	92,885,424 (GRCm38)	missense	unknown
R3730:Cfap54	UTSW	10	93,011,473 (GRCm38)	nonsense	probably null
R3770:Cfap54	UTSW	10	92,878,536 (GRCm38)	missense	unknown
R3776:Cfap54	UTSW	10	93,045,100 (GRCm38)	missense	probably damaging	1.00
R3778:Cfap54	UTSW	10	92,904,344 (GRCm38)	utr 3 prime	probably benign
R3795:Cfap54	UTSW	10	92,942,873 (GRCm38)	unclassified	probably benign
R3834:Cfap54	UTSW	10	92,801,123 (GRCm38)	splice site	probably benign
R3891:Cfap54	UTSW	10	93,038,846 (GRCm38)	missense	possibly damaging	0.87
R3932:Cfap54	UTSW	10	92,829,757 (GRCm38)	missense	probably benign	0.03
R3973:Cfap54	UTSW	10	92,839,471 (GRCm38)	missense	possibly damaging	0.95
R3974:Cfap54	UTSW	10	92,839,471 (GRCm38)	missense	possibly damaging	0.95
R3976:Cfap54	UTSW	10	92,839,471 (GRCm38)	missense	possibly damaging	0.95
R3978:Cfap54	UTSW	10	92,962,412 (GRCm38)	missense	probably benign	0.01
R4190:Cfap54	UTSW	10	92,885,023 (GRCm38)	missense	unknown
R4389:Cfap54	UTSW	10	92,967,500 (GRCm38)	missense	probably benign	0.37
R4542:Cfap54	UTSW	10	93,025,129 (GRCm38)	missense	probably benign	0.12
R4564:Cfap54	UTSW	10	92,839,540 (GRCm38)	unclassified	probably benign
R4576:Cfap54	UTSW	10	93,043,228 (GRCm38)	critical splice donor site	probably null
R4620:Cfap54	UTSW	10	92,969,757 (GRCm38)	missense	probably benign	0.01
R4714:Cfap54	UTSW	10	92,815,918 (GRCm38)	missense	probably benign	0.01
R4762:Cfap54	UTSW	10	93,061,453 (GRCm38)	splice site	probably null
R4776:Cfap54	UTSW	10	92,972,694 (GRCm38)	missense	possibly damaging	0.96
R4819:Cfap54	UTSW	10	92,836,477 (GRCm38)	nonsense	probably null
R4827:Cfap54	UTSW	10	92,902,075 (GRCm38)	utr 3 prime	probably benign
R4832:Cfap54	UTSW	10	92,967,528 (GRCm38)	missense	probably benign	0.01
R4965:Cfap54	UTSW	10	93,066,799 (GRCm38)	missense	probably benign	0.23
R5001:Cfap54	UTSW	10	92,964,534 (GRCm38)	missense	probably benign	0.01
R5060:Cfap54	UTSW	10	93,039,151 (GRCm38)	missense	probably damaging	1.00
R5067:Cfap54	UTSW	10	93,066,766 (GRCm38)	missense	probably benign	0.17
R5069:Cfap54	UTSW	10	92,937,774 (GRCm38)	missense	probably benign
R5094:Cfap54	UTSW	10	92,898,999 (GRCm38)	utr 3 prime	probably benign
R5109:Cfap54	UTSW	10	92,937,891 (GRCm38)	missense	probably benign	0.03
R5127:Cfap54	UTSW	10	92,886,387 (GRCm38)	splice site	probably null
R5143:Cfap54	UTSW	10	93,029,158 (GRCm38)	missense	possibly damaging	0.73
R5147:Cfap54	UTSW	10	92,937,838 (GRCm38)	missense	probably benign	0.00
R5158:Cfap54	UTSW	10	93,065,197 (GRCm38)	missense	probably damaging	1.00
R5256:Cfap54	UTSW	10	93,045,023 (GRCm38)	splice site	probably null
R5256:Cfap54	UTSW	10	92,935,091 (GRCm38)	nonsense	probably null
R5266:Cfap54	UTSW	10	92,815,902 (GRCm38)	missense	probably benign	0.16
R5304:Cfap54	UTSW	10	92,821,106 (GRCm38)	missense	probably damaging	0.97
R5369:Cfap54	UTSW	10	93,061,257 (GRCm38)	intron	probably benign
R5406:Cfap54	UTSW	10	93,001,858 (GRCm38)	missense	probably benign	0.33
R5471:Cfap54	UTSW	10	93,028,660 (GRCm38)	missense	probably damaging	1.00
R5485:Cfap54	UTSW	10	93,029,117 (GRCm38)	missense	probably damaging	1.00
R5540:Cfap54	UTSW	10	92,972,608 (GRCm38)	missense	possibly damaging	0.85
R5586:Cfap54	UTSW	10	92,972,611 (GRCm38)	nonsense	probably null
R5614:Cfap54	UTSW	10	93,045,049 (GRCm38)	missense	probably damaging	1.00
R5634:Cfap54	UTSW	10	92,904,263 (GRCm38)	critical splice donor site	probably benign
R5680:Cfap54	UTSW	10	92,979,017 (GRCm38)	nonsense	probably null
R5797:Cfap54	UTSW	10	92,967,576 (GRCm38)	missense	probably benign	0.11
R5859:Cfap54	UTSW	10	93,016,524 (GRCm38)	nonsense	probably null
R5878:Cfap54	UTSW	10	92,964,561 (GRCm38)	missense	probably benign	0.01
R5910:Cfap54	UTSW	10	93,065,181 (GRCm38)	missense	probably damaging	0.99
R5936:Cfap54	UTSW	10	92,962,412 (GRCm38)	missense	probably benign	0.01
R5994:Cfap54	UTSW	10	93,039,081 (GRCm38)	missense	probably damaging	0.99
R6080:Cfap54	UTSW	10	93,045,335 (GRCm38)	missense	possibly damaging	0.64
R6268:Cfap54	UTSW	10	93,038,909 (GRCm38)	missense	probably damaging	1.00
R6296:Cfap54	UTSW	10	93,066,846 (GRCm38)	missense	probably damaging	1.00
R6409:Cfap54	UTSW	10	92,967,492 (GRCm38)	missense	probably benign	0.04
R6545:Cfap54	UTSW	10	92,836,457 (GRCm38)	missense	probably benign	0.31
R6570:Cfap54	UTSW	10	92,815,958 (GRCm38)	missense	unknown
R6597:Cfap54	UTSW	10	92,999,040 (GRCm38)	missense	possibly damaging	0.85
R6702:Cfap54	UTSW	10	92,868,734 (GRCm38)	missense	unknown
R6703:Cfap54	UTSW	10	92,868,734 (GRCm38)	missense	unknown
R6720:Cfap54	UTSW	10	92,821,119 (GRCm38)	missense	probably benign	0.07
R6841:Cfap54	UTSW	10	92,875,015 (GRCm38)	missense	unknown
R6910:Cfap54	UTSW	10	92,836,512 (GRCm38)	missense	probably benign	0.29
R6953:Cfap54	UTSW	10	92,994,678 (GRCm38)	missense	probably benign	0.19
R7009:Cfap54	UTSW	10	92,875,019 (GRCm38)	missense	unknown
R7129:Cfap54	UTSW	10	93,016,571 (GRCm38)	missense	probably benign	0.06
R7171:Cfap54	UTSW	10	92,776,210 (GRCm38)	missense	probably damaging	0.99
R7189:Cfap54	UTSW	10	92,937,728 (GRCm38)	missense	unknown
R7225:Cfap54	UTSW	10	92,904,374 (GRCm38)	missense	unknown
R7270:Cfap54	UTSW	10	92,839,458 (GRCm38)	missense	probably benign	0.03
R7323:Cfap54	UTSW	10	92,801,138 (GRCm38)	missense	probably benign	0.00
R7380:Cfap54	UTSW	10	93,047,978 (GRCm38)	missense	probably damaging	1.00
R7395:Cfap54	UTSW	10	92,884,703 (GRCm38)	missense	unknown
R7411:Cfap54	UTSW	10	92,868,755 (GRCm38)	missense	unknown
R7503:Cfap54	UTSW	10	92,887,436 (GRCm38)	splice site	probably null
R7622:Cfap54	UTSW	10	92,956,944 (GRCm38)	missense	unknown
R7679:Cfap54	UTSW	10	92,967,512 (GRCm38)	missense	probably benign	0.01
R7776:Cfap54	UTSW	10	92,868,741 (GRCm38)	missense	unknown
R7844:Cfap54	UTSW	10	92,902,058 (GRCm38)	missense	unknown
R7980:Cfap54	UTSW	10	92,982,060 (GRCm38)	missense	possibly damaging	0.95
R7988:Cfap54	UTSW	10	92,902,079 (GRCm38)	missense	unknown
R8101:Cfap54	UTSW	10	92,884,796 (GRCm38)	missense	unknown
R8119:Cfap54	UTSW	10	92,868,810 (GRCm38)	missense	unknown
R8134:Cfap54	UTSW	10	92,878,516 (GRCm38)	missense	unknown
R8168:Cfap54	UTSW	10	92,908,877 (GRCm38)	missense	unknown
R8179:Cfap54	UTSW	10	92,997,316 (GRCm38)	missense	possibly damaging	0.68
R8392:Cfap54	UTSW	10	92,962,417 (GRCm38)	missense	unknown
R8436:Cfap54	UTSW	10	92,964,536 (GRCm38)	missense	unknown
R8505:Cfap54	UTSW	10	92,978,993 (GRCm38)	missense	probably benign	0.03
R8671:Cfap54	UTSW	10	92,955,072 (GRCm38)	missense	unknown
R8716:Cfap54	UTSW	10	92,964,632 (GRCm38)	missense	probably benign	0.00
R8816:Cfap54	UTSW	10	92,878,592 (GRCm38)	missense	unknown
R8822:Cfap54	UTSW	10	93,039,141 (GRCm38)	missense	probably benign	0.09
R8827:Cfap54	UTSW	10	92,938,248 (GRCm38)	missense	unknown
R8920:Cfap54	UTSW	10	92,940,337 (GRCm38)	critical splice acceptor site	probably null
R8924:Cfap54	UTSW	10	93,001,823 (GRCm38)	missense	probably damaging	0.99
R8954:Cfap54	UTSW	10	93,043,393 (GRCm38)	missense	probably damaging	1.00
R8963:Cfap54	UTSW	10	93,028,700 (GRCm38)	nonsense	probably null
R9010:Cfap54	UTSW	10	92,899,059 (GRCm38)	missense	unknown
R9017:Cfap54	UTSW	10	92,816,021 (GRCm38)	missense	probably benign	0.07
R9093:Cfap54	UTSW	10	92,815,908 (GRCm38)	missense	probably benign	0.03
R9095:Cfap54	UTSW	10	93,011,020 (GRCm38)	missense	probably damaging	1.00
R9142:Cfap54	UTSW	10	92,984,235 (GRCm38)	missense	possibly damaging	0.87
R9178:Cfap54	UTSW	10	92,994,717 (GRCm38)	missense	probably benign	0.10
R9196:Cfap54	UTSW	10	93,037,891 (GRCm38)	missense	probably benign	0.22
R9203:Cfap54	UTSW	10	93,045,128 (GRCm38)	missense	probably benign	0.30
R9258:Cfap54	UTSW	10	92,935,098 (GRCm38)	missense	unknown
R9275:Cfap54	UTSW	10	93,039,186 (GRCm38)	missense	possibly damaging	0.86
R9287:Cfap54	UTSW	10	92,969,703 (GRCm38)	missense	possibly damaging	0.50
R9289:Cfap54	UTSW	10	92,821,074 (GRCm38)	missense	possibly damaging	0.83
R9310:Cfap54	UTSW	10	92,962,315 (GRCm38)	missense	unknown
R9397:Cfap54	UTSW	10	92,997,285 (GRCm38)	missense	probably damaging	0.96
R9462:Cfap54	UTSW	10	92,902,058 (GRCm38)	missense	unknown
R9697:Cfap54	UTSW	10	92,956,989 (GRCm38)	missense	unknown
R9746:Cfap54	UTSW	10	92,801,219 (GRCm38)	missense	probably benign	0.03
R9755:Cfap54	UTSW	10	92,921,368 (GRCm38)	missense	unknown
X0022:Cfap54	UTSW	10	92,932,614 (GRCm38)	missense	probably damaging	1.00
X0022:Cfap54	UTSW	10	92,878,603 (GRCm38)	missense	unknown
X0027:Cfap54	UTSW	10	93,001,888 (GRCm38)	missense	possibly damaging	0.86
X0027:Cfap54	UTSW	10	92,878,538 (GRCm38)	missense	unknown
Z1177:Cfap54	UTSW	10	92,979,026 (GRCm38)	missense	probably damaging	1.00

Predicted Primers

PCR Primer
(F):5'- TATGGGCAGCAAAGCTCTG -3'
(R):5'- CTCTTCACAGTGAATTGAAAGGG -3'

Sequencing Primer
(F):5'- ATGGACTCCAAGCTCTCCTG -3'
(R):5'- GGTGCCCTTTTCTATAAGGA -3'

Posted On

2019-05-15