Mutagenetix > Incidental Mutation

Incidental Mutation 'R7133:Gpr25'

552792

Institutional Source

Beutler Lab

Gene Symbol

Gpr25

Ensembl Gene

ENSMUSG00000052759

Gene Name

G protein-coupled receptor 25

Synonyms

LOC383563

MMRRC Submission

Accession Numbers

Essential gene?

Non essential (E-score: 0.000) question?

Stock #

R7133 (G1)

Quality Score

225.009

Status

Validated

Chromosome

Chromosomal Location

136259797-136260873 bp(-) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

T to A at 136260821 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Tyrosine to Phenylalanine at position 18 (Y18F)

Ref Sequence

ENSEMBL: ENSMUSP00000083583 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000086395]

AlphaFold

P0C5I1

Predicted Effect

probably damaging
Transcript: ENSMUST00000086395
AA Change: Y18F

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)

SMART Domains

Protein: ENSMUSP00000083583
Gene: ENSMUSG00000052759
AA Change: Y18F

Domain	Start	End	E-Value	Type
Pfam:7tm_1	56	304	1.1e-40	PFAM
low complexity region	328	342	N/A	INTRINSIC

Coding Region Coverage

1x: 100.0%
3x: 100.0%
10x: 99.7%
20x: 99.0%

Validation Efficiency

98% (91/93)

MGI Phenotype

FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] This gene is intronless and encodes a member of the G-protein coupled receptor 1 family. G-protein coupled receptors are membrane proteins which activate signaling cascades as a response to extracellular stress. This gene has been linked to arterial stiffness. [provided by RefSeq, Nov 2012]

Allele List at MGI

Other mutations in this stock

Total: 91 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
2610008E11Rik	T	A	10: 79,066,639	K614N	probably benign	Het
2610008E11Rik	T	G	10: 79,066,640	K614T	probably benign	Het
Ahsa1	T	A	12: 87,270,342	S120R	probably benign	Het
Anapc16	G	A	10: 59,996,480	T37I	possibly damaging	Het
Arid2	C	A	15: 96,378,875	P1606H	probably damaging	Het
Ash1l	TATCTCCTTTTCCAAAAA	TA	3: 88,983,457		probably null	Het
Asic1	T	A	15: 99,672,087	N96K	probably damaging	Het
Asph	A	T	4: 9,484,575	D582E	probably benign	Het
Astn1	A	G	1: 158,572,987	D528G	probably damaging	Het
Atp9b	A	G	18: 80,909,656	V164A		Het
Cbx6	C	T	15: 79,828,665	G187D	possibly damaging	Het
Cct7	A	G	6: 85,466,645	T332A	probably benign	Het
Clca4a	A	T	3: 144,961,890	L440*	probably null	Het
Crybg2	A	T	4: 134,065,443	I130F	probably benign	Het
Crybg3	T	C	16: 59,536,804	E798G	probably damaging	Het
Ctnnd2	A	G	15: 30,480,849	E81G	possibly damaging	Het
Cwc22	C	T	2: 77,929,478	R75H	possibly damaging	Het
Cym	T	G	3: 107,214,214	D254A	probably damaging	Het
D1Ertd622e	G	A	1: 97,645,920	P140L	probably benign	Het
Dcaf11	T	A	14: 55,568,926		probably null	Het
Dctn1	A	T	6: 83,180,044		probably null	Het
Dennd5a	C	T	7: 109,896,242		probably null	Het
Dmpk	T	A	7: 19,087,307	C217S	probably damaging	Het
Dnah1	C	A	14: 31,286,076	V2125L	probably benign	Het
Exoc4	G	A	6: 33,438,473	A427T	probably benign	Het
Farp2	G	T	1: 93,621,234	V1021F	probably damaging	Het
Flg2	T	C	3: 93,219,762	S1994P	unknown	Het
Fmnl1	T	C	11: 103,181,784		probably null	Het
Frem2	T	A	3: 53,572,339	I1978F	possibly damaging	Het
Gan	T	A	8: 117,187,230	C122*	probably null	Het
Hoxa7	C	T	6: 52,215,740	E223K	probably benign	Het
Hypk	A	G	2: 121,453,480		probably null	Het
Ibsp	A	T	5: 104,302,306	K27*	probably null	Het
Ighv6-5	T	A	12: 114,416,775	T41S	probably benign	Het
Ighv9-4	T	C	12: 114,300,137	M59V	probably benign	Het
Il17rb	T	C	14: 29,996,871	D418G	probably damaging	Het
Ints5	T	A	19: 8,895,559	V294E	probably damaging	Het
Irx6	G	A	8: 92,678,413	C303Y	probably damaging	Het
Itih3	T	A	14: 30,917,698	I389F	probably damaging	Het
Lama1	C	T	17: 67,782,146	T1604I		Het
Lama3	C	A	18: 12,539,786	Q873K	probably benign	Het
Lhx1	T	G	11: 84,519,920	S284R	probably benign	Het
Lrba	T	A	3: 86,394,931		probably null	Het
Lrg1	C	T	17: 56,120,592	G127R	possibly damaging	Het
Mapre1	T	A	2: 153,764,963	L205H	probably benign	Het
Meltf	A	G	16: 31,892,799	N581S	probably damaging	Het
Mgat5	A	C	1: 127,365,189	M149L	probably benign	Het
Myh1	A	G	11: 67,202,586	T168A	probably benign	Het
Naalad2	C	T	9: 18,327,377	V681I	probably benign	Het
Olfr584	T	A	7: 103,085,998	L155Q	probably damaging	Het
Olfr78	A	G	7: 102,742,317	S229P	probably damaging	Het
Pcnx2	T	C	8: 125,801,504	T1326A	probably benign	Het
Peg3	A	T	7: 6,708,945	C1093S	probably damaging	Het
Pidd1	T	C	7: 141,439,900	S650G	probably benign	Het
Pik3c2b	T	C	1: 133,090,234	S945P	possibly damaging	Het
Plec	T	C	15: 76,176,027	T3237A	possibly damaging	Het
Prpf3	A	T	3: 95,833,740		probably null	Het
Prpf4b	C	A	13: 34,901,494	H974Q	probably benign	Het
Ptpn3	T	C	4: 57,225,863	T451A	probably benign	Het
Ptprs	A	G	17: 56,417,429	Y1577H	probably damaging	Het
Rgr	C	T	14: 37,048,925	M1I	probably null	Het
Rxrg	A	T	1: 167,631,109	N257I	probably benign	Het
Sbno2	T	C	10: 80,086,312	D9G	probably damaging	Het
Scd1	T	C	19: 44,406,595	K64E	probably damaging	Het
Serpinb1a	T	A	13: 32,850,325	I28F	possibly damaging	Het
Sfmbt2	A	T	2: 10,402,007	E39V	probably damaging	Het
Slc29a1	A	G	17: 45,589,971	M89T	possibly damaging	Het
Slco4a1	T	A	2: 180,472,063	V431E	possibly damaging	Het
Smg1	C	T	7: 118,152,908	C2698Y	unknown	Het
Sptlc2	C	T	12: 87,350,377	D212N	probably benign	Het
St6galnac1	T	C	11: 116,767,073	T334A	possibly damaging	Het
Stim2	A	T	5: 53,998,921	D13V	possibly damaging	Het
Syne1	A	G	10: 5,231,592	W4248R	probably damaging	Het
Tanc1	T	C	2: 59,797,609	Y584H	probably benign	Het
Thoc6	C	A	17: 23,673,660		probably null	Het
Trim30a	T	A	7: 104,429,326	N181I	possibly damaging	Het
Trip11	T	A	12: 101,884,070	Q1245L	probably damaging	Het
Tshz3	A	G	7: 36,770,569	D661G	probably benign	Het
Ttll8	C	A	15: 88,915,427	V604L	probably damaging	Het
Ubtfl1	A	G	9: 18,409,635	D153G	probably damaging	Het
Ufsp2	C	A	8: 45,983,624	N137K	probably benign	Het
Ugt2a3	A	G	5: 87,325,534	I508T	possibly damaging	Het
Uso1	G	A	5: 92,158,465	E94K	probably benign	Het
Usp54	A	G	14: 20,561,242	S1169P	probably benign	Het
Vmn1r235	C	T	17: 21,262,030	P206S	probably benign	Het
Vmn1r69	T	C	7: 10,581,068		probably benign	Het
Zfp263	T	A	16: 3,749,391	C523*	probably null	Het
Zfp799	G	T	17: 32,820,236	T352K	probably benign	Het
Zfr	T	A	15: 12,180,638	V951E	probably damaging	Het
Zfyve26	T	C	12: 79,284,152	D431G	probably benign	Het
Zswim9	C	A	7: 13,259,737	A831S	probably damaging	Het

Other mutations in Gpr25

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL03028:Gpr25	APN	1	136260812	missense	probably benign	0.00
R1728:Gpr25	UTSW	1	136260710	missense	probably benign
R1729:Gpr25	UTSW	1	136260710	missense	probably benign
R1732:Gpr25	UTSW	1	136260128	missense	probably benign	0.01
R1739:Gpr25	UTSW	1	136260710	missense	probably benign
R1762:Gpr25	UTSW	1	136260710	missense	probably benign
R1783:Gpr25	UTSW	1	136260710	missense	probably benign
R1785:Gpr25	UTSW	1	136260710	missense	probably benign
R1907:Gpr25	UTSW	1	136260800	missense	probably benign	0.00
R2877:Gpr25	UTSW	1	136260815	missense	possibly damaging	0.86
R3081:Gpr25	UTSW	1	136259885	missense	possibly damaging	0.68
R5132:Gpr25	UTSW	1	136260365	missense	probably damaging	1.00
R8163:Gpr25	UTSW	1	136259858	missense	probably damaging	1.00
R8248:Gpr25	UTSW	1	136260677	missense	probably benign	0.03
R9051:Gpr25	UTSW	1	136260288	missense	probably benign	0.00

Predicted Primers

PCR Primer
(F):5'- AGCTGCTGACTTTGCACAG -3'
(R):5'- TGGAAGGTAACATCACCACC -3'

Sequencing Primer
(F):5'- TGACTTTGCACAGGCCGTC -3'
(R):5'- AAAGCCTCGGCCTAGGGTG -3'

Posted On

2019-05-15