Mutagenetix > Incidental Mutations

Incidental Mutation 'R7137:Zfp445'

553138

Institutional Source

Beutler Lab

Gene Symbol

Zfp445

Ensembl Gene

ENSMUSG00000047036

Gene Name

zinc finger protein 445

Synonyms

ZNF168

MMRRC Submission

Accession Numbers

Is this an essential gene?

Possibly essential (E-score: 0.513) question?

Stock #

R7137 (G1)

Quality Score

225.009

Status

Not validated

Chromosome

Chromosomal Location

122844529-122866006 bp(-) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

T to C at 122854778 bp

Zygosity

Heterozygous

Amino Acid Change

Glutamic Acid to Glycine at position 272 (E272G)

Ref Sequence

ENSEMBL: ENSMUSP00000055738 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000056467] [ENSMUST00000213971] [ENSMUST00000214626] [ENSMUST00000216063]

Predicted Effect

probably damaging
Transcript: ENSMUST00000056467
AA Change: E272G

PolyPhen 2 Score 0.996 (Sensitivity: 0.55; Specificity: 0.98)

SMART Domains

Protein: ENSMUSP00000055738
Gene: ENSMUSG00000047036
AA Change: E272G

Domain	Start	End	E-Value	Type
SCAN	48	160	1.07e-59	SMART
KRAB	219	278	6.74e-30	SMART
low complexity region	320	334	N/A	INTRINSIC
low complexity region	419	430	N/A	INTRINSIC
ZnF_C2H2	470	492	2.09e-3	SMART
ZnF_C2H2	498	520	3.16e-3	SMART
ZnF_C2H2	553	575	1.41e0	SMART
ZnF_C2H2	581	603	1.04e-3	SMART
ZnF_C2H2	634	656	1.6e-4	SMART
ZnF_C2H2	662	686	6.78e-3	SMART
ZnF_C2H2	718	740	1.67e-2	SMART
ZnF_C2H2	746	768	1.2e-3	SMART
ZnF_C2H2	796	818	2.02e-1	SMART
ZnF_C2H2	824	846	2.95e-3	SMART
ZnF_C2H2	933	955	2.49e-1	SMART
ZnF_C2H2	961	983	4.61e-5	SMART

Predicted Effect

probably damaging
Transcript: ENSMUST00000213971
AA Change: E98G

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)

Predicted Effect

probably damaging
Transcript: ENSMUST00000214626
AA Change: E272G

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)

Predicted Effect

probably damaging
Transcript: ENSMUST00000216063
AA Change: E272G

PolyPhen 2 Score 0.996 (Sensitivity: 0.55; Specificity: 0.98)

Meta Mutation Damage Score

0.3297

Coding Region Coverage

1x: 100.0%
3x: 99.9%
10x: 99.6%
20x: 98.9%

Validation Efficiency

Allele List at MGI

Other mutations in this stock

Total: 51 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
A2m	T	C	6: 121,677,985	S1423P	possibly damaging	Het
Acvr1c	A	G	2: 58,283,387		probably null	Het
Adgrf5	A	G	17: 43,450,897	K1161R	probably damaging	Het
Arhgap32	T	G	9: 32,151,936	D80E	probably benign	Het
Aspg	T	C	12: 112,112,198	V30A	possibly damaging	Het
Bcl2a1d	T	C	9: 88,731,478	D81G	probably damaging	Het
Cep170b	T	C	12: 112,735,167	V160A	probably benign	Het
Ces1c	T	C	8: 93,130,842	Y37C	probably benign	Het
Cntnap5a	T	C	1: 116,089,376	L233P	probably damaging	Het
Crem	C	A	18: 3,273,459	A245S	possibly damaging	Het
Cyp2d12	C	T	15: 82,557,821	A280V	probably benign	Het
Dnah2	C	T	11: 69,491,555	G1243D	probably damaging	Het
Emcn	T	G	3: 137,403,991	N131K	probably damaging	Het
Fat3	A	T	9: 15,997,148	D2519E	probably damaging	Het
Fibin	T	A	2: 110,362,656	D47V	probably damaging	Het
Fsd1	G	A	17: 55,993,876	R245H	probably damaging	Het
Gimap8	C	A	6: 48,650,253	L54I	probably damaging	Het
Gm20481	A	G	17: 34,970,095	Y27C	unknown	Het
Gm21319	A	T	12: 87,773,546	L81Q	possibly damaging	Het
Grin1	T	G	2: 25,313,538	M154L	probably benign	Het
Ighv8-13	A	G	12: 115,765,577	L20P	probably damaging	Het
Insc	G	A	7: 114,811,615	V236I	probably benign	Het
Lrrk1	A	G	7: 66,285,279	F1031L	probably benign	Het
Man2a2	C	A	7: 80,359,751	R785L	probably benign	Het
Mcur1	C	A	13: 43,544,455		probably null	Het
Med12l	A	C	3: 59,258,254	R1464S	probably damaging	Het
Mycbp2	A	T	14: 103,282,679	M767K	possibly damaging	Het
Naalad2	T	C	9: 18,323,487	I762V	probably benign	Het
Nfkbid	A	G	7: 30,426,256	T357A	possibly damaging	Het
Pcdh17	A	G	14: 84,533,549	R1156G	possibly damaging	Het
Pcdhb1	T	C	18: 37,267,392	S799P	possibly damaging	Het
Pde3a	A	T	6: 141,498,746	E1093D	probably benign	Het
Plcg1	T	C	2: 160,753,926	Y572H	possibly damaging	Het
Plxna4	A	G	6: 32,517,264	L139P	probably damaging	Het
Psma1	A	G	7: 114,274,448	Y6H	probably damaging	Het
Psmd2	A	G	16: 20,652,627	E76G	probably benign	Het
Ptk2	G	A	15: 73,221,809	P854S	possibly damaging	Het
Pyy	T	G	11: 102,107,273	D27A	possibly damaging	Het
Slc22a2	A	G	17: 12,584,341	T21A	probably benign	Het
Slc25a2	T	C	18: 37,638,147	I110V	probably benign	Het
Sult1c2	A	C	17: 53,838,394	W85G	probably damaging	Het
Svs1	A	G	6: 48,990,149	Y677C	probably damaging	Het
Syt12	C	T	19: 4,453,950	D218N	probably damaging	Het
Tln1	C	T	4: 43,540,616	V1462M	probably damaging	Het
Tor1aip2	T	A	1: 156,051,976	N33K	possibly damaging	Het
Usp17lb	C	A	7: 104,841,591	W43L	probably benign	Het
Vmn1r76	A	G	7: 11,930,685	Y201H	possibly damaging	Het
Wnk1	C	A	6: 120,038,212		probably benign	Het
Wrnip1	A	G	13: 32,802,749	D171G	probably benign	Het
Zar1	T	A	5: 72,580,816	N81I	probably damaging	Het
Zbtb45	T	C	7: 13,007,156	T392A	probably benign	Het

Other mutations in Zfp445

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL02427:Zfp445	APN	9	122852230	missense	probably benign	0.02
IGL02608:Zfp445	APN	9	122861875	missense	probably damaging	0.98
IGL03216:Zfp445	APN	9	122851978	missense	probably damaging	0.99
IGL03218:Zfp445	APN	9	122857529	missense	probably benign	0.00
Nonpareil	UTSW	9	122852345	missense	probably benign	0.02
R0080:Zfp445	UTSW	9	122852356	missense	probably damaging	0.98
R0082:Zfp445	UTSW	9	122852356	missense	probably damaging	0.98
R0453:Zfp445	UTSW	9	122853513	missense	possibly damaging	0.92
R0610:Zfp445	UTSW	9	122852981	missense	probably benign	0.44
R0730:Zfp445	UTSW	9	122861758	missense	probably damaging	1.00
R1622:Zfp445	UTSW	9	122852549	missense	possibly damaging	0.90
R1719:Zfp445	UTSW	9	122852642	missense	probably damaging	1.00
R2108:Zfp445	UTSW	9	122852240	missense	probably benign	0.13
R2117:Zfp445	UTSW	9	122853437	nonsense	probably null
R2143:Zfp445	UTSW	9	122853482	missense	possibly damaging	0.70
R2162:Zfp445	UTSW	9	122852476	missense	probably damaging	0.99
R3620:Zfp445	UTSW	9	122852768	missense	probably benign
R3621:Zfp445	UTSW	9	122852768	missense	probably benign
R3745:Zfp445	UTSW	9	122854726	missense	probably benign	0.00
R3829:Zfp445	UTSW	9	122853077	missense	probably benign
R3831:Zfp445	UTSW	9	122852476	missense	probably damaging	0.99
R4172:Zfp445	UTSW	9	122851937	missense	probably benign	0.01
R4180:Zfp445	UTSW	9	122852524	missense	probably benign	0.00
R4747:Zfp445	UTSW	9	122857150	missense	possibly damaging	0.81
R4923:Zfp445	UTSW	9	122852293	missense	probably benign
R5010:Zfp445	UTSW	9	122852345	missense	probably benign	0.02
R5578:Zfp445	UTSW	9	122853337	missense	probably benign	0.00
R5759:Zfp445	UTSW	9	122853146	missense	probably benign	0.00
R5864:Zfp445	UTSW	9	122853487	missense	probably benign	0.00
R5865:Zfp445	UTSW	9	122853487	missense	probably benign	0.00
R5987:Zfp445	UTSW	9	122853886	missense	probably benign
R6481:Zfp445	UTSW	9	122857566	missense	probably benign	0.00
R6738:Zfp445	UTSW	9	122862058	missense	probably damaging	0.96
R6917:Zfp445	UTSW	9	122862294	splice site	probably null
R7224:Zfp445	UTSW	9	122852143	missense	probably benign	0.28
R8056:Zfp445	UTSW	9	122851967	missense	possibly damaging	0.95
R8263:Zfp445	UTSW	9	122852813	missense	probably benign	0.00
R8313:Zfp445	UTSW	9	122853630	missense	possibly damaging	0.48

Predicted Primers

PCR Primer
(F):5'- AGAAAGTGGCCCAGGGTTCTAG -3'
(R):5'- TCTTAGGGCTATCACAAATGGCTG -3'

Sequencing Primer
(F):5'- GGTTCTAGCACCCCTTAGTAAG -3'
(R):5'- GGCCAGGCATTCTGTCCTAG -3'

Posted On

2019-05-15