Mutagenetix > Incidental Mutation

Incidental Mutation 'R7141:Mrgprb1'

553339

Institutional Source

Beutler Lab

Gene Symbol

Mrgprb1

Ensembl Gene

ENSMUSG00000070547

Gene Name

MAS-related GPR, member B1

Synonyms

MrgB1

MMRRC Submission

045221-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.048) question?

Stock #

R7141 (G1)

Quality Score

225.009

Status

Validated

Chromosome

Chromosomal Location

48444113-48456342 bp(-) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

A to G at 48447687 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Valine to Alanine at position 159 (V159A)

Ref Sequence

ENSEMBL: ENSMUSP00000091946 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000094384] [ENSMUST00000188095] [ENSMUST00000188918]

AlphaFold

Q3UG61

Predicted Effect

possibly damaging
Transcript: ENSMUST00000094384
AA Change: V159A

PolyPhen 2 Score 0.613 (Sensitivity: 0.87; Specificity: 0.91)

SMART Domains

Protein: ENSMUSP00000091946
Gene: ENSMUSG00000070547
AA Change: V159A

Domain	Start	End	E-Value	Type
Pfam:7TM_GPCR_Srx	50	227	5.5e-11	PFAM
Pfam:7tm_1	59	290	4.3e-10	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000188095

Predicted Effect

probably benign
Transcript: ENSMUST00000188918

SMART Domains

Protein: ENSMUSP00000140432
Gene: ENSMUSG00000070547

Domain	Start	End	E-Value	Type
SCOP:d1l9ha_	23	84	3e-6	SMART

Coding Region Coverage

1x: 100.0%
3x: 99.9%
10x: 99.6%
20x: 98.9%

Validation Efficiency

100% (56/56)

Allele List at MGI

Other mutations in this stock

Total: 58 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Aamp	A	C	1: 74,284,111 (GRCm38)		probably null	Het
Abhd10	C	T	16: 45,742,806 (GRCm38)	R29Q	probably benign	Het
Adgrv1	A	G	13: 81,492,501 (GRCm38)	Y3369H	probably benign	Het
Agl	A	G	3: 116,753,286 (GRCm38)	I1305T	probably benign	Het
Apba3	A	G	10: 81,273,055 (GRCm38)	I551V	probably damaging	Het
Appbp2	A	T	11: 85,191,751 (GRCm38)	Y551*	probably null	Het
Btbd17	T	C	11: 114,791,815 (GRCm38)	N357S	possibly damaging	Het
Casp2	T	C	6: 42,280,395 (GRCm38)	F426S	possibly damaging	Het
Cdh5	A	G	8: 104,113,001 (GRCm38)	N35D	probably benign	Het
Cep350	G	T	1: 155,914,748 (GRCm38)	Q1354K	probably damaging	Het
Cep44	A	G	8: 56,539,851 (GRCm38)	C243R	probably damaging	Het
Chst4	A	T	8: 110,030,839 (GRCm38)	S131T	probably damaging	Het
Cox15	T	C	19: 43,736,747 (GRCm38)	N406D	probably benign	Het
Cttnbp2	C	T	6: 18,380,468 (GRCm38)	R1467H	probably benign	Het
Dock8	A	G	19: 25,181,620 (GRCm38)	D1714G	probably null	Het
Erbb2	G	T	11: 98,427,309 (GRCm38)	R457L	probably damaging	Het
Esyt3	T	C	9: 99,321,440 (GRCm38)	N463S	probably benign	Het
Fam171a1	C	T	2: 3,225,152 (GRCm38)	Q441*	probably null	Het
Fam186b	T	A	15: 99,283,892 (GRCm38)	M142L	probably benign	Het
Git2	G	T	5: 114,769,698 (GRCm38)	C35*	probably null	Het
Gm21103	T	G	14: 6,301,807 (GRCm38)	Q202P	probably damaging	Het
Gm5622	G	T	14: 51,655,882 (GRCm38)	E89*	probably null	Het
Hmcn2	C	A	2: 31,360,896 (GRCm38)	T790K	probably benign	Het
Hspg2	T	A	4: 137,552,116 (GRCm38)	L3114H	probably damaging	Het
Igkv3-10	A	T	6: 70,572,981 (GRCm38)	Q37L	possibly damaging	Het
Kcp	G	T	6: 29,487,512 (GRCm38)	Y1106*	probably null	Het
Khsrp	T	C	17: 57,025,602 (GRCm38)	D226G	possibly damaging	Het
Klre1	T	A	6: 129,583,166 (GRCm38)	W134R	probably damaging	Het
Lrrc66	A	G	5: 73,629,977 (GRCm38)	I10T	probably benign	Het
Map4	T	C	9: 109,978,870 (GRCm38)	M1T	probably null	Het
Met	T	A	6: 17,527,155 (GRCm38)	I535K	probably benign	Het
Mmut	T	C	17: 40,952,839 (GRCm38)	V500A	possibly damaging	Het
Mrpl41	A	G	2: 24,974,456 (GRCm38)	L68P	probably damaging	Het
Mtmr4	T	C	11: 87,600,613 (GRCm38)	W135R	probably damaging	Het
Myh10	A	G	11: 68,802,139 (GRCm38)	D1420G	probably benign	Het
Naglu	A	C	11: 101,072,230 (GRCm38)	D229A	probably benign	Het
Naip5	T	A	13: 100,219,830 (GRCm38)	E1092D	probably benign	Het
Ncln	G	T	10: 81,487,849 (GRCm38)	Y517*	probably null	Het
Nr1h4	G	A	10: 89,498,229 (GRCm38)	R100*	probably null	Het
Or5k1	G	A	16: 58,797,408 (GRCm38)	T146M	probably benign	Het
P2rx5	A	T	11: 73,160,648 (GRCm38)	T18S	probably damaging	Het
Pclo	T	C	5: 14,679,257 (GRCm38)	S2710P	unknown	Het
Pdhx	A	T	2: 103,073,314 (GRCm38)	F46I	probably benign	Het
Piezo2	A	T	18: 63,145,110 (GRCm38)	L241*	probably null	Het
Pitpnm1	G	A	19: 4,102,787 (GRCm38)	V65M	probably damaging	Het
Rgs3	A	G	4: 62,690,487 (GRCm38)	D330G	probably damaging	Het
Scaf8	T	C	17: 3,159,182 (GRCm38)	V60A	unknown	Het
Sema4c	A	G	1: 36,553,020 (GRCm38)	Y249H	probably damaging	Het
Spp2	T	C	1: 88,407,328 (GRCm38)	Y27H	probably damaging	Het
Sugct	T	A	13: 17,644,787 (GRCm38)	I158F	possibly damaging	Het
Sympk	A	T	7: 19,054,092 (GRCm38)	I1178F	probably benign	Het
Tmppe	T	C	9: 114,404,968 (GRCm38)	Y112H	probably benign	Het
Trp53bp2	A	G	1: 182,448,508 (GRCm38)	T187A		Het
Tspoap1	A	G	11: 87,774,697 (GRCm38)	S754G	probably damaging	Het
Vmn1r214	C	T	13: 23,034,669 (GRCm38)	A111V	probably benign	Het
Vmn2r70	G	C	7: 85,558,836 (GRCm38)	S811C	probably benign	Het
Zfp638	T	C	6: 83,867,199 (GRCm38)	S15P	unknown	Het
Zfp763	A	G	17: 33,018,795 (GRCm38)	S459P	probably damaging	Het

Other mutations in Mrgprb1

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00540:Mrgprb1	APN	7	48,447,543 (GRCm38)	missense	probably damaging	0.99
IGL01141:Mrgprb1	APN	7	48,448,027 (GRCm38)	missense	probably benign	0.36
IGL01393:Mrgprb1	APN	7	48,448,006 (GRCm38)	missense	possibly damaging	0.48
IGL02430:Mrgprb1	APN	7	48,447,661 (GRCm38)	missense	possibly damaging	0.95
IGL02485:Mrgprb1	APN	7	48,447,717 (GRCm38)	missense	possibly damaging	0.88
R0026:Mrgprb1	UTSW	7	48,447,204 (GRCm38)	missense	possibly damaging	0.66
R0051:Mrgprb1	UTSW	7	48,447,214 (GRCm38)	missense	probably benign	0.01
R0789:Mrgprb1	UTSW	7	48,456,184 (GRCm38)	splice site	probably benign
R1223:Mrgprb1	UTSW	7	48,447,687 (GRCm38)	missense	possibly damaging	0.61
R1327:Mrgprb1	UTSW	7	48,447,429 (GRCm38)	missense	possibly damaging	0.87
R1456:Mrgprb1	UTSW	7	48,448,029 (GRCm38)	missense	probably damaging	0.98
R1561:Mrgprb1	UTSW	7	48,447,125 (GRCm38)	splice site	probably null
R1567:Mrgprb1	UTSW	7	48,447,453 (GRCm38)	missense	probably damaging	0.97
R2030:Mrgprb1	UTSW	7	48,447,328 (GRCm38)	missense	possibly damaging	0.83
R2165:Mrgprb1	UTSW	7	48,447,322 (GRCm38)	missense	probably benign	0.00
R2885:Mrgprb1	UTSW	7	48,447,721 (GRCm38)	missense	probably damaging	1.00
R3108:Mrgprb1	UTSW	7	48,447,328 (GRCm38)	missense	possibly damaging	0.93
R3919:Mrgprb1	UTSW	7	48,448,081 (GRCm38)	missense	probably benign	0.03
R4021:Mrgprb1	UTSW	7	48,447,123 (GRCm38)	missense	possibly damaging	0.95
R4613:Mrgprb1	UTSW	7	48,447,708 (GRCm38)	missense	possibly damaging	0.91
R4809:Mrgprb1	UTSW	7	48,447,991 (GRCm38)	missense	possibly damaging	0.89
R5249:Mrgprb1	UTSW	7	48,447,477 (GRCm38)	missense	possibly damaging	0.91
R5425:Mrgprb1	UTSW	7	48,447,971 (GRCm38)	missense	possibly damaging	0.81
R5555:Mrgprb1	UTSW	7	48,447,775 (GRCm38)	missense	probably benign	0.06
R5595:Mrgprb1	UTSW	7	48,447,684 (GRCm38)	missense	probably damaging	0.99
R5982:Mrgprb1	UTSW	7	48,447,820 (GRCm38)	missense	probably benign	0.01
R6746:Mrgprb1	UTSW	7	48,447,897 (GRCm38)	missense	possibly damaging	0.82
R7066:Mrgprb1	UTSW	7	48,447,676 (GRCm38)	missense	probably benign	0.27
R7633:Mrgprb1	UTSW	7	48,447,583 (GRCm38)	missense	probably benign	0.01
R8072:Mrgprb1	UTSW	7	48,448,147 (GRCm38)	nonsense	probably null
R8080:Mrgprb1	UTSW	7	48,446,910 (GRCm38)	splice site	probably null
R8112:Mrgprb1	UTSW	7	48,447,934 (GRCm38)	missense	probably damaging	0.97
R8493:Mrgprb1	UTSW	7	48,447,573 (GRCm38)	missense	probably damaging	0.99
R8817:Mrgprb1	UTSW	7	48,447,322 (GRCm38)	missense	probably benign	0.01
R9135:Mrgprb1	UTSW	7	48,447,298 (GRCm38)	missense	possibly damaging	0.95

Predicted Primers

PCR Primer
(F):5'- TAAGCACCAAGGCCAGACTG -3'
(R):5'- AGGAATGCCTTCTCTGTCTACATC -3'

Sequencing Primer
(F):5'- GCCAGACTGGATCCCAAGAG -3'
(R):5'- ACATCCTCAACCTTGCTGG -3'

Posted On

2019-05-15