Mutagenetix > Incidental Mutation

Incidental Mutation 'R0600:Golgb1'

55344

Institutional Source

Beutler Lab

Gene Symbol

Golgb1

Ensembl Gene

ENSMUSG00000034243

Gene Name

golgi autoantigen, golgin subfamily b, macrogolgin 1

Synonyms

Giantin, C130074L01Rik, F730017E11Rik, Gm6840, 6330407A06Rik

MMRRC Submission

038789-MU

Accession Numbers

Essential gene?

Probably essential (E-score: 0.911) question?

Stock #

R0600 (G1)

Quality Score

225

Status

Validated

Chromosome

Chromosomal Location

36875140-36933085 bp(+) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

T to A at 36916271 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Leucine to Glutamine at position 1960 (L1960Q)

Ref Sequence

ENSEMBL: ENSMUSP00000110460 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000039855] [ENSMUST00000114812]

AlphaFold

no structure available at present

Predicted Effect

probably damaging
Transcript: ENSMUST00000039855
AA Change: L2001Q

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)

SMART Domains

Protein: ENSMUSP00000045239
Gene: ENSMUSG00000034243
AA Change: L2001Q

Domain	Start	End	E-Value	Type
internal_repeat_2	24	61	7.47e-6	PROSPERO
low complexity region	87	107	N/A	INTRINSIC
coiled coil region	130	219	N/A	INTRINSIC
low complexity region	491	512	N/A	INTRINSIC
internal_repeat_3	519	558	7.47e-6	PROSPERO
coiled coil region	563	594	N/A	INTRINSIC
internal_repeat_4	627	661	3.38e-5	PROSPERO
coiled coil region	679	1121	N/A	INTRINSIC
coiled coil region	1153	1240	N/A	INTRINSIC
internal_repeat_4	1253	1288	3.38e-5	PROSPERO
low complexity region	1300	1314	N/A	INTRINSIC
internal_repeat_1	1321	1352	3.51e-6	PROSPERO
low complexity region	1357	1369	N/A	INTRINSIC
coiled coil region	1402	1755	N/A	INTRINSIC
internal_repeat_2	1760	1798	7.47e-6	PROSPERO
internal_repeat_3	1761	1804	7.47e-6	PROSPERO
coiled coil region	1818	2034	N/A	INTRINSIC
low complexity region	2291	2306	N/A	INTRINSIC
internal_repeat_1	2351	2382	3.51e-6	PROSPERO
low complexity region	2400	2418	N/A	INTRINSIC
low complexity region	2538	2549	N/A	INTRINSIC
coiled coil region	2775	2827	N/A	INTRINSIC
coiled coil region	2854	2943	N/A	INTRINSIC
low complexity region	2964	2976	N/A	INTRINSIC
coiled coil region	3007	3057	N/A	INTRINSIC
coiled coil region	3117	3163	N/A	INTRINSIC
transmembrane domain	3215	3237	N/A	INTRINSIC

Predicted Effect

probably damaging
Transcript: ENSMUST00000114812
AA Change: L1960Q

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)

SMART Domains

Protein: ENSMUSP00000110460
Gene: ENSMUSG00000034243
AA Change: L1960Q

Domain	Start	End	E-Value	Type
internal_repeat_2	24	61	6.71e-6	PROSPERO
low complexity region	87	107	N/A	INTRINSIC
low complexity region	120	134	N/A	INTRINSIC
low complexity region	143	154	N/A	INTRINSIC
low complexity region	200	219	N/A	INTRINSIC
low complexity region	450	471	N/A	INTRINSIC
internal_repeat_3	478	517	6.71e-6	PROSPERO
coiled coil region	522	553	N/A	INTRINSIC
internal_repeat_4	586	620	3.05e-5	PROSPERO
coiled coil region	638	1080	N/A	INTRINSIC
coiled coil region	1112	1199	N/A	INTRINSIC
internal_repeat_4	1212	1247	3.05e-5	PROSPERO
low complexity region	1259	1273	N/A	INTRINSIC
internal_repeat_1	1280	1311	3.14e-6	PROSPERO
low complexity region	1316	1328	N/A	INTRINSIC
coiled coil region	1361	1714	N/A	INTRINSIC
internal_repeat_2	1719	1757	6.71e-6	PROSPERO
internal_repeat_3	1720	1763	6.71e-6	PROSPERO
coiled coil region	1777	1993	N/A	INTRINSIC
low complexity region	2250	2265	N/A	INTRINSIC
internal_repeat_1	2310	2341	3.14e-6	PROSPERO
low complexity region	2359	2377	N/A	INTRINSIC
low complexity region	2497	2508	N/A	INTRINSIC
coiled coil region	2734	2786	N/A	INTRINSIC
coiled coil region	2813	2902	N/A	INTRINSIC
low complexity region	2923	2935	N/A	INTRINSIC
coiled coil region	2966	3016	N/A	INTRINSIC
coiled coil region	3076	3122	N/A	INTRINSIC
transmembrane domain	3174	3196	N/A	INTRINSIC

Meta Mutation Damage Score

0.2017

Coding Region Coverage

1x: 99.3%
3x: 98.7%
10x: 97.1%
20x: 93.5%

Validation Efficiency

99% (95/96)

MGI Phenotype

PHENOTYPE: Homozygous knockout affects glycosylation of glycoproteins in the extra-cellular matrix of the palatal shelves, resulting in their failure to elevate and fuse, leading to cleft palate. [provided by MGI curators]

Allele List at MGI

All alleles(7) : Targeted, other(2) Gene trapped(5)

Other mutations in this stock

Total: 92 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
2310057M21Rik	A	G	7: 131,357,660 (GRCm38)	S150P	probably damaging	Het
4932431P20Rik	T	A	7: 29,533,265 (GRCm38)		noncoding transcript	Het
5530400C23Rik	T	G	6: 133,293,211 (GRCm38)		probably benign	Het
Ahctf1	A	C	1: 179,763,468 (GRCm38)		probably null	Het
Ang5	T	C	14: 43,962,749 (GRCm38)	V90A	probably benign	Het
Ano9	C	T	7: 141,104,710 (GRCm38)	G442R	probably damaging	Het
Apaf1	G	A	10: 91,060,052 (GRCm38)	T386I	probably damaging	Het
Apob	C	A	12: 8,006,440 (GRCm38)	H1608N	probably damaging	Het
Arhgap12	C	A	18: 6,064,433 (GRCm38)		probably benign	Het
Asxl1	T	A	2: 153,399,904 (GRCm38)	D791E	probably benign	Het
Avl9	T	C	6: 56,736,906 (GRCm38)	V383A	probably benign	Het
Btbd1	A	C	7: 81,816,006 (GRCm38)	D197E	probably damaging	Het
C87499	A	T	4: 88,629,299 (GRCm38)	I45K	probably damaging	Het
Camta2	T	C	11: 70,673,959 (GRCm38)	I938V	possibly damaging	Het
Cdca7	C	A	2: 72,483,467 (GRCm38)	A200D	possibly damaging	Het
Cep104	A	T	4: 154,006,792 (GRCm38)	Y923F	possibly damaging	Het
Cep135	G	C	5: 76,621,305 (GRCm38)	V601L	probably benign	Het
Ces2b	G	A	8: 104,835,910 (GRCm38)	G291S	probably benign	Het
Col6a6	C	T	9: 105,761,440 (GRCm38)	G1400D	probably damaging	Het
Cyth2	T	C	7: 45,813,117 (GRCm38)	E1G	probably damaging	Het
Dand5	A	T	8: 84,816,292 (GRCm38)	L185Q	probably damaging	Het
Dck	T	C	5: 88,781,221 (GRCm38)	V253A	probably benign	Het
Ddx20	A	G	3: 105,679,080 (GRCm38)	S650P	probably damaging	Het
Dicer1	G	A	12: 104,706,864 (GRCm38)	P799S	probably damaging	Het
Dst	C	T	1: 34,189,119 (GRCm38)	P1606L	probably damaging	Het
Eya2	G	A	2: 165,769,237 (GRCm38)	C477Y	probably damaging	Het
Fam208b	A	T	13: 3,576,054 (GRCm38)	F1299I	probably benign	Het
Fip1l1	T	A	5: 74,595,842 (GRCm38)	N498K	probably damaging	Het
Flt4	C	T	11: 49,636,339 (GRCm38)		probably benign	Het
Galntl6	T	C	8: 57,837,183 (GRCm38)		probably null	Het
Gda	A	T	19: 21,434,303 (GRCm38)	F44I	possibly damaging	Het
Gli2	G	A	1: 118,840,389 (GRCm38)	R703C	probably damaging	Het
Gm14085	A	T	2: 122,514,398 (GRCm38)	I162F	probably damaging	Het
Gramd1b	T	C	9: 40,308,355 (GRCm38)	D341G	probably damaging	Het
Grid2	G	T	6: 63,503,435 (GRCm38)	A78S	probably benign	Het
Hao2	A	T	3: 98,883,560 (GRCm38)		probably benign	Het
Hook3	A	G	8: 26,118,986 (GRCm38)	V10A	probably benign	Het
Kif20a	A	G	18: 34,629,209 (GRCm38)	E425G	probably damaging	Het
Lrp1	T	C	10: 127,567,383 (GRCm38)	D2107G	probably benign	Het
Lrriq3	T	C	3: 155,187,736 (GRCm38)	I358T	possibly damaging	Het
Mad2l2	A	G	4: 148,140,924 (GRCm38)	D17G	possibly damaging	Het
Mastl	G	T	2: 23,133,346 (GRCm38)	T455K	probably benign	Het
Mkln1	G	T	6: 31,432,927 (GRCm38)		probably benign	Het
Mmp1b	A	T	9: 7,387,947 (GRCm38)	Y16N	possibly damaging	Het
Mmp24	C	T	2: 155,792,597 (GRCm38)	A79V	probably benign	Het
Mrps35	T	A	6: 147,070,734 (GRCm38)	C292S	possibly damaging	Het
Myom1	T	C	17: 71,120,648 (GRCm38)	F1435L	possibly damaging	Het
Nars2	C	T	7: 97,039,923 (GRCm38)	H351Y	probably damaging	Het
Nat2	A	T	8: 67,501,267 (GRCm38)	I10F	probably damaging	Het
Nfix	G	A	8: 84,726,526 (GRCm38)	R300C	probably damaging	Het
Olfm5	A	T	7: 104,153,869 (GRCm38)	Y462*	probably null	Het
Olfr1228	C	A	2: 89,249,398 (GRCm38)	E87*	probably null	Het
Olfr1339	C	T	4: 118,734,789 (GRCm38)	H87Y	probably damaging	Het
Olfr1508	T	C	14: 52,463,509 (GRCm38)	I167V	probably benign	Het
Olfr322	C	A	11: 58,666,160 (GRCm38)	F200L	probably damaging	Het
Olfr340	C	A	2: 36,452,648 (GRCm38)	A21E	probably benign	Het
Olfr44	A	T	9: 39,484,988 (GRCm38)	F85L	probably benign	Het
Olfr495	T	A	7: 108,395,231 (GRCm38)	I37N	probably damaging	Het
Olfr855	T	C	9: 19,585,304 (GRCm38)	S256P	possibly damaging	Het
Olfr926	T	A	9: 38,877,815 (GRCm38)	I213N	probably damaging	Het
Otog	A	T	7: 46,251,395 (GRCm38)		probably benign	Het
Pdcd2l	A	T	7: 34,192,807 (GRCm38)	D212E	possibly damaging	Het
Pex5	T	C	6: 124,404,637 (GRCm38)	N213S	probably benign	Het
Pkn3	C	T	2: 30,081,134 (GRCm38)	P238S	probably benign	Het
Prl2b1	A	T	13: 27,390,740 (GRCm38)		probably null	Het
Ptprb	A	T	10: 116,368,807 (GRCm38)	I1849L	possibly damaging	Het
Rasal3	G	T	17: 32,393,526 (GRCm38)	S787Y	probably damaging	Het
Scn2a	T	A	2: 65,701,833 (GRCm38)	D596E	possibly damaging	Het
Sdhd	A	T	9: 50,603,764 (GRCm38)	V9D	possibly damaging	Het
Serinc5	T	C	13: 92,708,057 (GRCm38)	S436P	probably damaging	Het
Slc27a1	C	T	8: 71,584,164 (GRCm38)	P348L	probably damaging	Het
Smg1	G	A	7: 118,160,383 (GRCm38)		probably benign	Het
Sorl1	A	T	9: 42,043,900 (GRCm38)		probably benign	Het
Sprtn	T	A	8: 124,900,218 (GRCm38)	H112Q	probably damaging	Het
Tet2	A	G	3: 133,467,602 (GRCm38)	M1633T	probably benign	Het
Tet2	T	A	3: 133,467,725 (GRCm38)	D1592V	probably benign	Het
Tmem68	A	T	4: 3,569,667 (GRCm38)	C8S	probably damaging	Het
Tnrc6a	T	A	7: 123,171,816 (GRCm38)	I943N	probably benign	Het
Trib2	A	T	12: 15,794,068 (GRCm38)	V191D	probably damaging	Het
Tsc22d4	T	C	5: 137,762,655 (GRCm38)	S113P	probably damaging	Het
Ttc21b	T	C	2: 66,239,570 (GRCm38)	R250G	probably damaging	Het
Ubr2	T	C	17: 46,967,248 (GRCm38)	Y721C	probably damaging	Het
Ubtfl1	A	T	9: 18,409,364 (GRCm38)	I63F	probably damaging	Het
Ush1c	G	A	7: 46,224,908 (GRCm38)	P171S	probably benign	Het
Utp20	A	T	10: 88,767,461 (GRCm38)	N1843K	probably damaging	Het
Vangl1	A	G	3: 102,166,937 (GRCm38)	Y285H	probably damaging	Het
Virma	A	G	4: 11,498,769 (GRCm38)	D70G	probably damaging	Het
Vmn2r102	T	C	17: 19,678,015 (GRCm38)	F431L	probably benign	Het
Wdr17	A	G	8: 54,661,495 (GRCm38)	I662T	probably damaging	Het
Wisp2	G	A	2: 163,825,313 (GRCm38)	C78Y	probably damaging	Het
Zfp160	G	A	17: 21,027,006 (GRCm38)	R606H	probably benign	Het
Zfp369	C	T	13: 65,296,434 (GRCm38)	R464C	probably damaging	Het

Other mutations in Golgb1

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01394:Golgb1	APN	16	36,931,564 (GRCm38)	missense	probably damaging	1.00
IGL01717:Golgb1	APN	16	36,915,502 (GRCm38)	nonsense	probably null
IGL01965:Golgb1	APN	16	36,917,920 (GRCm38)	missense	probably damaging	1.00
IGL02128:Golgb1	APN	16	36,916,304 (GRCm38)	missense	probably damaging	1.00
IGL02268:Golgb1	APN	16	36,913,128 (GRCm38)	missense	probably benign	0.25
IGL02383:Golgb1	APN	16	36,886,200 (GRCm38)	missense	probably benign	0.01
IGL02444:Golgb1	APN	16	36,907,816 (GRCm38)	splice site	probably benign
IGL02635:Golgb1	APN	16	36,915,013 (GRCm38)	missense	probably benign	0.00
IGL02655:Golgb1	APN	16	36,918,080 (GRCm38)	missense	probably damaging	0.98
IGL02887:Golgb1	APN	16	36,925,849 (GRCm38)	missense	probably damaging	0.99
IGL02937:Golgb1	APN	16	36,916,210 (GRCm38)	missense	probably damaging	1.00
IGL02973:Golgb1	APN	16	36,912,080 (GRCm38)	missense	possibly damaging	0.92
IGL02982:Golgb1	APN	16	36,925,810 (GRCm38)	missense	probably damaging	0.98
IGL03065:Golgb1	APN	16	36,912,866 (GRCm38)	missense	probably benign	0.11
IGL03109:Golgb1	APN	16	36,915,611 (GRCm38)	missense	possibly damaging	0.93
IGL03323:Golgb1	APN	16	36,913,453 (GRCm38)	nonsense	probably null
I2288:Golgb1	UTSW	16	36,898,542 (GRCm38)	missense	probably benign	0.00
I2289:Golgb1	UTSW	16	36,898,542 (GRCm38)	missense	probably benign	0.00
R0071:Golgb1	UTSW	16	36,915,503 (GRCm38)	missense	probably benign	0.00
R0071:Golgb1	UTSW	16	36,915,503 (GRCm38)	missense	probably benign	0.00
R0080:Golgb1	UTSW	16	36,898,611 (GRCm38)	missense	probably damaging	1.00
R0102:Golgb1	UTSW	16	36,875,468 (GRCm38)	intron	probably benign
R0242:Golgb1	UTSW	16	36,875,630 (GRCm38)	nonsense	probably null
R0242:Golgb1	UTSW	16	36,875,630 (GRCm38)	nonsense	probably null
R0276:Golgb1	UTSW	16	36,913,876 (GRCm38)	missense	probably damaging	1.00
R0394:Golgb1	UTSW	16	36,875,579 (GRCm38)	intron	probably benign
R0469:Golgb1	UTSW	16	36,931,635 (GRCm38)	missense	probably benign	0.41
R0522:Golgb1	UTSW	16	36,915,205 (GRCm38)	frame shift	probably null
R0575:Golgb1	UTSW	16	36,918,809 (GRCm38)	missense	probably benign
R0608:Golgb1	UTSW	16	36,916,330 (GRCm38)	nonsense	probably null
R0711:Golgb1	UTSW	16	36,918,790 (GRCm38)	missense	probably damaging	1.00
R0785:Golgb1	UTSW	16	36,898,790 (GRCm38)	missense	possibly damaging	0.95
R0893:Golgb1	UTSW	16	36,912,277 (GRCm38)	missense	possibly damaging	0.64
R1163:Golgb1	UTSW	16	36,916,126 (GRCm38)	missense	possibly damaging	0.50
R1208:Golgb1	UTSW	16	36,915,205 (GRCm38)	frame shift	probably null
R1315:Golgb1	UTSW	16	36,914,900 (GRCm38)	missense	probably benign	0.40
R1429:Golgb1	UTSW	16	36,900,563 (GRCm38)	missense	possibly damaging	0.93
R1505:Golgb1	UTSW	16	36,919,643 (GRCm38)	missense	possibly damaging	0.79
R1537:Golgb1	UTSW	16	36,898,788 (GRCm38)	missense	possibly damaging	0.89
R1610:Golgb1	UTSW	16	36,926,101 (GRCm38)	missense	probably benign	0.25
R1659:Golgb1	UTSW	16	36,887,617 (GRCm38)	missense	probably benign	0.01
R1769:Golgb1	UTSW	16	36,916,001 (GRCm38)	missense	probably damaging	1.00
R2105:Golgb1	UTSW	16	36,914,664 (GRCm38)	missense	probably benign
R2212:Golgb1	UTSW	16	36,887,347 (GRCm38)	missense	probably damaging	1.00
R2261:Golgb1	UTSW	16	36,893,360 (GRCm38)	missense	probably damaging	1.00
R2352:Golgb1	UTSW	16	36,898,559 (GRCm38)	missense	probably damaging	0.99
R2357:Golgb1	UTSW	16	36,912,008 (GRCm38)	missense	probably damaging	1.00
R2400:Golgb1	UTSW	16	36,918,466 (GRCm38)	missense	possibly damaging	0.62
R2513:Golgb1	UTSW	16	36,915,151 (GRCm38)	missense	possibly damaging	0.73
R3103:Golgb1	UTSW	16	36,894,849 (GRCm38)	missense	probably damaging	1.00
R3413:Golgb1	UTSW	16	36,887,347 (GRCm38)	missense	probably damaging	1.00
R3748:Golgb1	UTSW	16	36,918,912 (GRCm38)	missense	probably benign	0.00
R3847:Golgb1	UTSW	16	36,898,733 (GRCm38)	missense	probably benign	0.00
R3850:Golgb1	UTSW	16	36,898,733 (GRCm38)	missense	probably benign	0.00
R3936:Golgb1	UTSW	16	36,914,056 (GRCm38)	nonsense	probably null
R3975:Golgb1	UTSW	16	36,918,571 (GRCm38)	missense	probably damaging	0.99
R4025:Golgb1	UTSW	16	36,915,344 (GRCm38)	missense	probably benign	0.00
R4369:Golgb1	UTSW	16	36,916,907 (GRCm38)	missense	probably damaging	1.00
R4518:Golgb1	UTSW	16	36,929,263 (GRCm38)	missense	probably damaging	0.98
R4600:Golgb1	UTSW	16	36,918,625 (GRCm38)	missense	probably damaging	1.00
R4610:Golgb1	UTSW	16	36,918,625 (GRCm38)	missense	probably damaging	1.00
R4660:Golgb1	UTSW	16	36,887,618 (GRCm38)	missense	probably damaging	0.99
R4811:Golgb1	UTSW	16	36,891,419 (GRCm38)	missense	probably damaging	1.00
R4815:Golgb1	UTSW	16	36,913,115 (GRCm38)	missense	possibly damaging	0.79
R4835:Golgb1	UTSW	16	36,891,407 (GRCm38)	missense	possibly damaging	0.86
R4904:Golgb1	UTSW	16	36,893,386 (GRCm38)	missense	probably damaging	1.00
R4916:Golgb1	UTSW	16	36,916,118 (GRCm38)	missense	probably benign	0.05
R5121:Golgb1	UTSW	16	36,919,258 (GRCm38)	missense	probably damaging	0.99
R5133:Golgb1	UTSW	16	36,891,457 (GRCm38)	missense	possibly damaging	0.75
R5143:Golgb1	UTSW	16	36,898,689 (GRCm38)	missense	probably benign	0.09
R5185:Golgb1	UTSW	16	36,875,141 (GRCm38)	unclassified	probably benign
R5188:Golgb1	UTSW	16	36,918,465 (GRCm38)	missense	probably benign	0.13
R5260:Golgb1	UTSW	16	36,913,141 (GRCm38)	missense	probably benign	0.00
R5297:Golgb1	UTSW	16	36,875,616 (GRCm38)	intron	probably benign
R5386:Golgb1	UTSW	16	36,912,315 (GRCm38)	nonsense	probably null
R5438:Golgb1	UTSW	16	36,900,508 (GRCm38)	missense	probably benign	0.15
R5439:Golgb1	UTSW	16	36,900,508 (GRCm38)	missense	probably benign	0.15
R5494:Golgb1	UTSW	16	36,928,683 (GRCm38)	missense	possibly damaging	0.67
R5592:Golgb1	UTSW	16	36,925,763 (GRCm38)	missense	probably benign	0.02
R5740:Golgb1	UTSW	16	36,919,000 (GRCm38)	missense	probably damaging	0.99
R5862:Golgb1	UTSW	16	36,926,091 (GRCm38)	splice site	silent
R5928:Golgb1	UTSW	16	36,911,987 (GRCm38)	missense	probably damaging	1.00
R6009:Golgb1	UTSW	16	36,914,959 (GRCm38)	missense	probably damaging	1.00
R6062:Golgb1	UTSW	16	36,914,671 (GRCm38)	missense	possibly damaging	0.89
R6102:Golgb1	UTSW	16	36,912,865 (GRCm38)	missense	probably damaging	1.00
R6198:Golgb1	UTSW	16	36,893,395 (GRCm38)	missense	probably damaging	1.00
R6253:Golgb1	UTSW	16	36,915,622 (GRCm38)	missense	possibly damaging	0.77
R6254:Golgb1	UTSW	16	36,913,978 (GRCm38)	missense	probably damaging	0.99
R6321:Golgb1	UTSW	16	36,918,197 (GRCm38)	nonsense	probably null
R6700:Golgb1	UTSW	16	36,875,584 (GRCm38)	intron	probably benign
R6870:Golgb1	UTSW	16	36,918,203 (GRCm38)	missense	probably damaging	1.00
R6882:Golgb1	UTSW	16	36,913,990 (GRCm38)	missense	probably benign
R6944:Golgb1	UTSW	16	36,912,113 (GRCm38)	missense	probably benign
R7108:Golgb1	UTSW	16	36,913,721 (GRCm38)	missense	probably benign	0.01
R7124:Golgb1	UTSW	16	36,913,673 (GRCm38)	missense	probably benign	0.01
R7125:Golgb1	UTSW	16	36,917,963 (GRCm38)	missense	possibly damaging	0.85
R7187:Golgb1	UTSW	16	36,916,150 (GRCm38)	missense	probably benign	0.43
R7205:Golgb1	UTSW	16	36,875,301 (GRCm38)	missense	unknown
R7206:Golgb1	UTSW	16	36,913,749 (GRCm38)	missense	probably benign	0.41
R7233:Golgb1	UTSW	16	36,914,758 (GRCm38)	missense	possibly damaging	0.91
R7320:Golgb1	UTSW	16	36,915,951 (GRCm38)	nonsense	probably null
R7367:Golgb1	UTSW	16	36,898,546 (GRCm38)	missense	probably benign	0.00
R7408:Golgb1	UTSW	16	36,898,547 (GRCm38)	missense	probably damaging	0.98
R7419:Golgb1	UTSW	16	36,912,919 (GRCm38)	missense	possibly damaging	0.95
R7556:Golgb1	UTSW	16	36,915,793 (GRCm38)	missense	probably benign	0.03
R7599:Golgb1	UTSW	16	36,875,396 (GRCm38)	missense	unknown
R7673:Golgb1	UTSW	16	36,913,669 (GRCm38)	missense	probably benign	0.05
R7789:Golgb1	UTSW	16	36,875,399 (GRCm38)	missense	unknown
R7792:Golgb1	UTSW	16	36,918,730 (GRCm38)	missense	probably benign	0.43
R7830:Golgb1	UTSW	16	36,898,721 (GRCm38)	missense	possibly damaging	0.93
R7847:Golgb1	UTSW	16	36,931,920 (GRCm38)	missense	probably damaging	1.00
R7905:Golgb1	UTSW	16	36,913,685 (GRCm38)	missense	probably benign
R7944:Golgb1	UTSW	16	36,914,104 (GRCm38)	missense	probably benign	0.02
R7945:Golgb1	UTSW	16	36,914,104 (GRCm38)	missense	probably benign	0.02
R7950:Golgb1	UTSW	16	36,915,424 (GRCm38)	missense	probably benign	0.13
R8040:Golgb1	UTSW	16	36,913,479 (GRCm38)	missense	possibly damaging	0.85
R8077:Golgb1	UTSW	16	36,918,633 (GRCm38)	missense	probably damaging	0.99
R8181:Golgb1	UTSW	16	36,916,830 (GRCm38)	missense	probably damaging	1.00
R8370:Golgb1	UTSW	16	36,912,317 (GRCm38)	missense	probably benign	0.00
R8684:Golgb1	UTSW	16	36,914,402 (GRCm38)	missense	possibly damaging	0.92
R8725:Golgb1	UTSW	16	36,919,201 (GRCm38)	missense	probably damaging	1.00
R8727:Golgb1	UTSW	16	36,919,201 (GRCm38)	missense	probably damaging	1.00
R8738:Golgb1	UTSW	16	36,916,313 (GRCm38)	missense	probably damaging	1.00
R8785:Golgb1	UTSW	16	36,919,744 (GRCm38)	missense	probably damaging	0.99
R8824:Golgb1	UTSW	16	36,915,689 (GRCm38)	missense	probably benign
R8825:Golgb1	UTSW	16	36,919,447 (GRCm38)	missense	probably benign	0.00
R8940:Golgb1	UTSW	16	36,916,397 (GRCm38)	missense	probably damaging	1.00
R8962:Golgb1	UTSW	16	36,913,616 (GRCm38)	missense	probably damaging	1.00
R9245:Golgb1	UTSW	16	36,918,819 (GRCm38)	nonsense	probably null
R9365:Golgb1	UTSW	16	36,915,762 (GRCm38)	missense	probably damaging	1.00
R9612:Golgb1	UTSW	16	36,919,605 (GRCm38)	missense	probably benign	0.41
R9620:Golgb1	UTSW	16	36,919,449 (GRCm38)	missense	probably benign
R9691:Golgb1	UTSW	16	36,898,634 (GRCm38)	missense	probably damaging	1.00
R9747:Golgb1	UTSW	16	36,893,407 (GRCm38)	missense	probably damaging	1.00
V1662:Golgb1	UTSW	16	36,898,542 (GRCm38)	missense	probably benign	0.00
X0067:Golgb1	UTSW	16	36,914,303 (GRCm38)	nonsense	probably null
Z1088:Golgb1	UTSW	16	36,919,742 (GRCm38)	missense	probably damaging	1.00

Predicted Primers

PCR Primer
(F):5'- AGAGTCGGAAATGCGGAACCTTC -3'
(R):5'- GCTTTGGGTGTCATCGAGCAGTATC -3'

Sequencing Primer
(F):5'- TGCGGAACCTTCAAAGATGTG -3'
(R):5'- GTCATCGAGCAGTATCTTGAAGC -3'

Posted On

2013-07-11