Mutagenetix > Incidental Mutations

Incidental Mutation 'R7144:Pgbd5'

553647

Institutional Source

Beutler Lab

Gene Symbol

Pgbd5

Ensembl Gene

ENSMUSG00000050751

Gene Name

piggyBac transposable element derived 5

Synonyms

MMRRC Submission

Accession Numbers

Is this an essential gene?

Non essential (E-score: 0.000) question?

Stock #

R7144 (G1)

Quality Score

225.009

Status

Validated

Chromosome

Chromosomal Location

124369049-124439658 bp(-) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

A to T at 124374317 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Methionine to Lysine at position 400 (M400K)

Ref Sequence

ENSEMBL: ENSMUSP00000120984 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000052580] [ENSMUST00000136892] [ENSMUST00000140012] [ENSMUST00000172566]

AlphaFold

D3YZI9

Predicted Effect

possibly damaging
Transcript: ENSMUST00000052580
AA Change: M286K

PolyPhen 2 Score 0.828 (Sensitivity: 0.84; Specificity: 0.93)

SMART Domains

Protein: ENSMUSP00000054788
Gene: ENSMUSG00000050751
AA Change: M286K

Domain	Start	End	E-Value	Type
Pfam:DDE_Tnp_1_7	6	372	5e-86	PFAM
low complexity region	392	403	N/A	INTRINSIC

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000128312

Predicted Effect

possibly damaging
Transcript: ENSMUST00000136892
AA Change: M286K

PolyPhen 2 Score 0.828 (Sensitivity: 0.84; Specificity: 0.93)

SMART Domains

Protein: ENSMUSP00000123265
Gene: ENSMUSG00000050751
AA Change: M286K

Domain	Start	End	E-Value	Type
Pfam:DDE_Tnp_1_7	6	372	5e-86	PFAM
low complexity region	392	403	N/A	INTRINSIC

Predicted Effect

possibly damaging
Transcript: ENSMUST00000140012
AA Change: M400K

PolyPhen 2 Score 0.828 (Sensitivity: 0.84; Specificity: 0.93)

SMART Domains

Protein: ENSMUSP00000120984
Gene: ENSMUSG00000050751
AA Change: M400K

Domain	Start	End	E-Value	Type
low complexity region	9	22	N/A	INTRINSIC
low complexity region	47	60	N/A	INTRINSIC
Pfam:DDE_Tnp_1_7	120	486	5.6e-90	PFAM
low complexity region	506	517	N/A	INTRINSIC

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000140126

Predicted Effect

possibly damaging
Transcript: ENSMUST00000172566
AA Change: M309K

PolyPhen 2 Score 0.577 (Sensitivity: 0.88; Specificity: 0.91)

SMART Domains

Protein: ENSMUSP00000133560
Gene: ENSMUSG00000050751
AA Change: M309K

Domain	Start	End	E-Value	Type
Pfam:DDE_Tnp_1_7	29	395	2e-86	PFAM
low complexity region	415	426	N/A	INTRINSIC

Coding Region Coverage

1x: 100.0%
3x: 100.0%
10x: 99.8%
20x: 99.3%

Validation Efficiency

99% (95/96)

MGI Phenotype

FUNCTION: The piggyBac family of proteins, found in diverse animals, are transposases related to the transposase of the canonical piggyBac transposon from the moth, Trichoplusia ni. This family also includes genes in several genomes that appear to have been derived from the piggyBac transposons. This gene belongs to the subfamily of piggyBac transposable element derived (PGBD) genes. The PGBD proteins appear to be novel, with no obvious relationship to other transposases, or other known protein families. The exact function of this gene is not known. [provided by RefSeq, Jul 2008]

Allele List at MGI

Other mutations in this stock

Total: 96 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Abca16	A	T	7: 120,433,573	I272F	possibly damaging	Het
Adcy10	G	T	1: 165,510,370	M184I	probably benign	Het
Aldoa	G	T	7: 126,796,862	T124N	possibly damaging	Het
Ap4e1	T	A	2: 127,011,807	I55N	probably damaging	Het
Arhgap21	T	C	2: 20,865,387	T913A	probably benign	Het
Atrnl1	G	A	19: 58,042,352	E1309K	probably damaging	Het
BC034090	A	T	1: 155,242,031	C114S	probably damaging	Het
Brinp2	A	G	1: 158,295,424		probably null	Het
Ccna1	G	T	3: 55,045,699	H408Q	probably benign	Het
Cd209g	T	G	8: 4,135,189		probably benign	Het
Cdc20b	A	G	13: 113,083,371	I433V	probably benign	Het
Cdk2ap1	G	A	5: 124,354,358	P5L	probably damaging	Het
Cep128	T	C	12: 91,294,159	E310G	probably damaging	Het
Cflar	G	T	1: 58,753,848	V458F		Het
Clec4b2	A	G	6: 123,181,384	T70A	probably benign	Het
Cntnap5c	A	G	17: 58,286,888	T741A	probably benign	Het
Csf3r	A	T	4: 126,043,722	T800S	probably benign	Het
Csnk1g2	T	C	10: 80,637,899	Y67H	probably damaging	Het
Cyp2c67	A	T	19: 39,615,694	V406E	probably benign	Het
Dnah10	A	G	5: 124,822,942	D3868G	probably damaging	Het
Dnah7a	A	T	1: 53,698,708		probably null	Het
Dst	A	G	1: 34,152,243	N208S	probably damaging	Het
Echdc3	A	G	2: 6,206,413		probably null	Het
Edrf1	T	C	7: 133,637,849	S13P	probably benign	Het
Ephb1	T	C	9: 101,964,077	Y734C	probably damaging	Het
Eps8l1	A	G	7: 4,472,185	Y325C	probably damaging	Het
Evc2	A	G	5: 37,386,839	D644G	probably damaging	Het
Eya4	A	C	10: 23,173,045	D54E	probably benign	Het
Filip1	T	C	9: 79,820,213	S375G	possibly damaging	Het
Fmo9	A	G	1: 166,677,620	M68T	probably benign	Het
Gemin5	G	C	11: 58,141,663	P772A	probably benign	Het
Gle1	T	G	2: 29,943,793	C401G	probably damaging	Het
Gm14548	A	G	7: 3,897,616	V45A	probably damaging	Het
Gm7298	A	T	6: 121,761,587	I376F	probably damaging	Het
Gpr35	A	C	1: 92,982,631	I22L	probably benign	Het
Grin2b	T	G	6: 135,733,476	D1024A	possibly damaging	Het
Hmcn1	T	C	1: 150,663,873	N2956D	probably damaging	Het
Htt	T	C	5: 34,846,006	L1275P	probably damaging	Het
Ibtk	T	C	9: 85,743,691	D2G	probably benign	Het
Il16	T	A	7: 83,646,451	D1170V	probably damaging	Het
Iqgap3	T	A	3: 88,116,910	I1513N	probably damaging	Het
Kiz	T	G	2: 146,950,510		probably null	Het
Krt12	A	T	11: 99,416,013	*488K	probably null	Het
Lap3	A	T	5: 45,496,948	T83S	probably benign	Het
Lars2	T	A	9: 123,431,993	S410T	probably damaging	Het
Limch1	A	G	5: 67,017,658	T518A	probably benign	Het
Lrrc49	A	T	9: 60,615,156	S381T	probably damaging	Het
Lrrk2	A	G	15: 91,734,055	D919G	possibly damaging	Het
Mmp1a	A	T	9: 7,475,318	S363C	probably damaging	Het
Mrps22	A	C	9: 98,601,471		probably null	Het
Mybpc3	T	C	2: 91,134,604	I1066T	probably benign	Het
Myo10	A	T	15: 25,723,925	N215I	probably damaging	Het
Myocd	A	C	11: 65,218,648	L99R	probably damaging	Het
Nadk	T	G	4: 155,589,336	I394S	probably damaging	Het
Nadsyn1	T	C	7: 143,811,215	N251S	probably damaging	Het
Nbeal1	G	C	1: 60,237,151	V684L	probably benign	Het
Ncapd2	G	A	6: 125,176,670	P694L	probably benign	Het
Olfr1101	G	T	2: 86,988,820	R119S	probably damaging	Het
Olfr1243	T	C	2: 89,527,557	I284M	probably damaging	Het
Olfr317	A	C	11: 58,732,745	L140R	probably damaging	Het
Olfr705	T	A	7: 106,873,868	I126F	probably damaging	Het
Pcdhb13	T	A	18: 37,443,256	I229K	probably damaging	Het
Phactr3	A	G	2: 178,302,736	N409S	probably damaging	Het
Pik3c2g	C	A	6: 139,629,870	P305Q	probably damaging	Het
Pik3r4	G	A	9: 105,650,584	V379M	probably damaging	Het
Pkd1l2	A	T	8: 117,076,131	C250*	probably null	Het
Pramef17	A	C	4: 143,991,533	S447A	probably benign	Het
Rapgef6	A	C	11: 54,657,365	T792P	possibly damaging	Het
Rexo5	A	G	7: 119,805,191	D170G	probably damaging	Het
Rnf17	G	A	14: 56,512,332		probably null	Het
Sept11	A	G	5: 93,156,866	I181V	probably benign	Het
Serpina1e	T	G	12: 103,947,018	*414C	probably null	Het
Serpine1	C	A	5: 137,071,064	Q80H	probably damaging	Het
Sh3bp2	A	G	5: 34,561,631	N560S	probably benign	Het
Slc25a25	A	G	2: 32,419,166	F221S	probably damaging	Het
Spag17	G	T	3: 100,027,401		probably null	Het
Sspn	T	C	6: 145,961,155	L104P	probably damaging	Het
St18	A	C	1: 6,833,594	E693A	probably damaging	Het
St6galnac3	T	C	3: 153,411,532	I185V	possibly damaging	Het
St8sia1	T	C	6: 142,876,669	D156G	probably damaging	Het
Syne2	C	A	12: 76,005,378	S4092R	probably benign	Het
Tll1	T	A	8: 64,124,945	D76V	possibly damaging	Het
Tmco1	C	T	1: 167,308,453		probably benign	Het
Tnfaip3	T	A	10: 19,007,281	T179S	probably benign	Het
Trav16	T	C	14: 53,743,639	I95T	possibly damaging	Het
Trip12	A	T	1: 84,793,714	S280T	probably damaging	Het
Unc79	A	G	12: 103,142,626	M2166V	probably benign	Het
Vmn2r1	A	G	3: 64,089,941	I339M	probably damaging	Het
Vwa5b1	A	G	4: 138,605,431		probably null	Het
Washc4	A	T	10: 83,573,774		probably null	Het
Wisp1	T	A	15: 66,913,030	V184E	probably damaging	Het
Wiz	A	G	17: 32,357,628	S642P	possibly damaging	Het
Zeb2	T	A	2: 45,110,041	K60N	possibly damaging	Het
Zfat	T	A	15: 68,178,782	T797S	probably benign	Het
Zfp74	T	C	7: 29,935,165	K373E	probably damaging	Het
Zswim5	T	C	4: 116,975,976		probably null	Het

Other mutations in Pgbd5

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01642:Pgbd5	APN	8	124384202	missense	probably benign	0.00
IGL01669:Pgbd5	APN	8	124374399	missense	possibly damaging	0.86
IGL01759:Pgbd5	APN	8	124384379	missense	probably damaging	1.00
IGL01762:Pgbd5	APN	8	124370610	missense	probably damaging	1.00
IGL02398:Pgbd5	APN	8	124384518	missense	probably damaging	1.00
R0348:Pgbd5	UTSW	8	124434032	missense	probably damaging	0.98
R0702:Pgbd5	UTSW	8	124374255	missense	probably benign	0.21
R0981:Pgbd5	UTSW	8	124384293	nonsense	probably null
R1259:Pgbd5	UTSW	8	124370585	missense	probably damaging	0.98
R1598:Pgbd5	UTSW	8	124374287	missense	probably benign	0.26
R1609:Pgbd5	UTSW	8	124434011	missense	probably benign	0.00
R1742:Pgbd5	UTSW	8	124380307	missense	probably damaging	1.00
R1938:Pgbd5	UTSW	8	124374249	nonsense	probably null
R1985:Pgbd5	UTSW	8	124370592	missense	probably benign	0.00
R2169:Pgbd5	UTSW	8	124384624	critical splice acceptor site	probably null
R4573:Pgbd5	UTSW	8	124376227	nonsense	probably null
R4917:Pgbd5	UTSW	8	124370566	missense	probably benign	0.14
R4918:Pgbd5	UTSW	8	124370566	missense	probably benign	0.14
R4946:Pgbd5	UTSW	8	124370585	missense	possibly damaging	0.93
R5409:Pgbd5	UTSW	8	124371880	missense	probably damaging	1.00
R5885:Pgbd5	UTSW	8	124384466	missense	probably damaging	1.00
R5946:Pgbd5	UTSW	8	124374317	missense	possibly damaging	0.83
R6907:Pgbd5	UTSW	8	124380282	missense	probably damaging	0.97
R6986:Pgbd5	UTSW	8	124384473	missense	possibly damaging	0.56
R7342:Pgbd5	UTSW	8	124433970	missense	probably benign	0.36
R7475:Pgbd5	UTSW	8	124434011	missense	probably benign	0.00
R8934:Pgbd5	UTSW	8	124384259	missense	possibly damaging	0.93
R8960:Pgbd5	UTSW	8	124384436	missense	probably benign	0.04
R9238:Pgbd5	UTSW	8	124380191	missense	probably damaging	0.96
X0067:Pgbd5	UTSW	8	124371912	missense	probably damaging	1.00
Z1188:Pgbd5	UTSW	8	124380216	missense	probably damaging	1.00
Z1190:Pgbd5	UTSW	8	124380216	missense	probably damaging	1.00

Predicted Primers

PCR Primer
(F):5'- TGATGAGACCAAGCCACCTG -3'
(R):5'- CCTTCAGGTGTGCTCTTATAGAGG -3'

Sequencing Primer
(F):5'- GCTAAGAGCTTCGTGCAGC -3'
(R):5'- AAAGGCCTCAGCAGCTG -3'

Posted On

2019-05-15