Mutagenetix > Incidental Mutation

Incidental Mutation 'R7150:Cntrl'

554039

Institutional Source

Beutler Lab

Gene Symbol

Cntrl

Ensembl Gene

ENSMUSG00000057110

Gene Name

centriolin

Synonyms

IB3/5, b2b1468Clo, Cep1, 6720467O09Rik, Ma2a8, Cep110

MMRRC Submission

045252-MU

Accession Numbers

Essential gene?

Probably essential (E-score: 0.915) question?

Stock #

R7150 (G1)

Quality Score

225.009

Status

Validated

Chromosome

Chromosomal Location

34999504-35068834 bp(+) (GRCm39)

Type of Mutation

critical splice acceptor site

DNA Base Change (assembly)

A to T at 35055457 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Ref Sequence

ENSEMBL: ENSMUSP00000108655 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000028237] [ENSMUST00000113032] [ENSMUST00000113032] [ENSMUST00000113032] [ENSMUST00000113034] [ENSMUST00000113037] [ENSMUST00000124081] [ENSMUST00000156933]

AlphaFold

A2AL36

Predicted Effect

probably null
Transcript: ENSMUST00000028237

SMART Domains

Protein: ENSMUSP00000028237
Gene: ENSMUSG00000057110

Domain	Start	End	E-Value	Type
low complexity region	21	38	N/A	INTRINSIC
LRR	146	167	2.54e1	SMART
LRR	168	190	3.24e0	SMART
LRR	192	214	7.16e0	SMART
Blast:LRR	217	239	8e-6	BLAST
low complexity region	275	292	N/A	INTRINSIC
coiled coil region	437	800	N/A	INTRINSIC
coiled coil region	858	971	N/A	INTRINSIC
low complexity region	975	995	N/A	INTRINSIC
coiled coil region	998	1102	N/A	INTRINSIC
internal_repeat_1	1119	1132	1.95e-5	PROSPERO
low complexity region	1153	1161	N/A	INTRINSIC
low complexity region	1268	1301	N/A	INTRINSIC
coiled coil region	1320	1629	N/A	INTRINSIC
coiled coil region	1661	2155	N/A	INTRINSIC
low complexity region	2193	2208	N/A	INTRINSIC
internal_repeat_1	2252	2265	1.95e-5	PROSPERO
low complexity region	2289	2307	N/A	INTRINSIC

Predicted Effect

probably null
Transcript: ENSMUST00000113032

SMART Domains

Protein: ENSMUSP00000108655
Gene: ENSMUSG00000057110

Domain	Start	End	E-Value	Type
low complexity region	20	53	N/A	INTRINSIC
coiled coil region	72	381	N/A	INTRINSIC
coiled coil region	413	907	N/A	INTRINSIC
low complexity region	945	960	N/A	INTRINSIC
coiled coil region	989	1011	N/A	INTRINSIC
low complexity region	1041	1059	N/A	INTRINSIC

Predicted Effect

probably null
Transcript: ENSMUST00000113032

SMART Domains

Protein: ENSMUSP00000108655
Gene: ENSMUSG00000057110

Domain	Start	End	E-Value	Type
low complexity region	20	53	N/A	INTRINSIC
coiled coil region	72	381	N/A	INTRINSIC
coiled coil region	413	907	N/A	INTRINSIC
low complexity region	945	960	N/A	INTRINSIC
coiled coil region	989	1011	N/A	INTRINSIC
low complexity region	1041	1059	N/A	INTRINSIC

Predicted Effect

probably null
Transcript: ENSMUST00000113032

SMART Domains

Protein: ENSMUSP00000108655
Gene: ENSMUSG00000057110

Domain	Start	End	E-Value	Type
low complexity region	20	53	N/A	INTRINSIC
coiled coil region	72	381	N/A	INTRINSIC
coiled coil region	413	907	N/A	INTRINSIC
low complexity region	945	960	N/A	INTRINSIC
coiled coil region	989	1011	N/A	INTRINSIC
low complexity region	1041	1059	N/A	INTRINSIC

Predicted Effect

probably benign
Transcript: ENSMUST00000113034

SMART Domains

Protein: ENSMUSP00000108657
Gene: ENSMUSG00000057110

Domain	Start	End	E-Value	Type
coiled coil region	1	247	N/A	INTRINSIC
internal_repeat_3	261	278	5.68e-5	PROSPERO
coiled coil region	305	418	N/A	INTRINSIC
low complexity region	422	442	N/A	INTRINSIC
coiled coil region	445	549	N/A	INTRINSIC
internal_repeat_1	566	579	1.52e-6	PROSPERO
internal_repeat_2	568	596	2.75e-5	PROSPERO
low complexity region	600	608	N/A	INTRINSIC
internal_repeat_2	626	653	2.75e-5	PROSPERO
low complexity region	715	748	N/A	INTRINSIC
coiled coil region	767	1076	N/A	INTRINSIC
internal_repeat_3	1095	1112	5.68e-5	PROSPERO
low complexity region	1184	1224	N/A	INTRINSIC
low complexity region	1344	1356	N/A	INTRINSIC
low complexity region	1366	1388	N/A	INTRINSIC
low complexity region	1400	1415	N/A	INTRINSIC
low complexity region	1421	1432	N/A	INTRINSIC
low complexity region	1640	1655	N/A	INTRINSIC
internal_repeat_1	1699	1712	1.52e-6	PROSPERO
low complexity region	1736	1754	N/A	INTRINSIC

Predicted Effect

probably null
Transcript: ENSMUST00000113037

SMART Domains

Protein: ENSMUSP00000108660
Gene: ENSMUSG00000057110

Domain	Start	End	E-Value	Type
coiled coil region	1	247	N/A	INTRINSIC
internal_repeat_3	261	278	5.34e-5	PROSPERO
coiled coil region	305	548	N/A	INTRINSIC
internal_repeat_1	565	578	1.42e-6	PROSPERO
internal_repeat_2	567	595	2.58e-5	PROSPERO
low complexity region	599	607	N/A	INTRINSIC
internal_repeat_2	625	652	2.58e-5	PROSPERO
low complexity region	714	747	N/A	INTRINSIC
coiled coil region	766	1075	N/A	INTRINSIC
internal_repeat_3	1094	1111	5.34e-5	PROSPERO
low complexity region	1183	1223	N/A	INTRINSIC
low complexity region	1343	1355	N/A	INTRINSIC
low complexity region	1365	1387	N/A	INTRINSIC
low complexity region	1399	1414	N/A	INTRINSIC
low complexity region	1420	1431	N/A	INTRINSIC
low complexity region	1639	1654	N/A	INTRINSIC
internal_repeat_1	1698	1711	1.42e-6	PROSPERO
low complexity region	1735	1753	N/A	INTRINSIC

Predicted Effect

probably benign
Transcript: ENSMUST00000124081

SMART Domains

Protein: ENSMUSP00000143087
Gene: ENSMUSG00000057110

Domain	Start	End	E-Value	Type
coiled coil region	1	51	N/A	INTRINSIC

Predicted Effect

probably null
Transcript: ENSMUST00000156933

SMART Domains

Protein: ENSMUSP00000118731
Gene: ENSMUSG00000057110

Domain	Start	End	E-Value	Type
low complexity region	21	38	N/A	INTRINSIC
LRR	146	167	2.54e1	SMART
LRR	168	190	3.24e0	SMART
LRR	192	214	7.16e0	SMART
Blast:LRR	217	239	7e-6	BLAST
low complexity region	275	292	N/A	INTRINSIC
coiled coil region	437	800	N/A	INTRINSIC
coiled coil region	858	971	N/A	INTRINSIC
low complexity region	975	995	N/A	INTRINSIC
coiled coil region	998	1102	N/A	INTRINSIC
internal_repeat_1	1119	1132	1.65e-5	PROSPERO
low complexity region	1153	1161	N/A	INTRINSIC
low complexity region	1268	1301	N/A	INTRINSIC
coiled coil region	1320	1629	N/A	INTRINSIC
coiled coil region	1661	2155	N/A	INTRINSIC
low complexity region	2193	2208	N/A	INTRINSIC
internal_repeat_1	2252	2265	1.65e-5	PROSPERO
low complexity region	2289	2307	N/A	INTRINSIC

Meta Mutation Damage Score

0.9584

Coding Region Coverage

1x: 100.0%
3x: 99.9%
10x: 99.7%
20x: 99.2%

Validation Efficiency

98% (59/60)

MGI Phenotype

FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] This gene encodes a centrosomal protein required for the centrosome to function as a microtubule organizing center. The gene product is also associated with centrosome maturation. One version of stem cell myeloproliferative disorder is the result of a reciprocal translocation between chromosomes 8 and 9, with the breakpoint associated with fibroblast growth factor receptor 1 and centrosomal protein 1. [provided by RefSeq, Jul 2008]
PHENOTYPE: Mice homozygous for an ENU-induced allele exhibit cardiac defects, including double outlet right ventricle, atrial septal defects, ventricular septal defects, tricuspid valve stenosis and heart right ventricle hypoplasia, and develop kidney cysts and hydronephrosis. [provided by MGI curators]

Allele List at MGI

Other mutations in this stock

Total: 60 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
2810004N23Rik	T	C	8: 125,589,653 (GRCm39)	D20G	possibly damaging	Het
5430401F13Rik	T	C	6: 131,529,630 (GRCm39)	S75P	probably benign	Het
Acad8	A	T	9: 26,889,750 (GRCm39)	F315I	probably damaging	Het
Adcy5	A	G	16: 35,118,904 (GRCm39)	Y1085C	probably damaging	Het
Aldh1a7	T	C	19: 20,693,382 (GRCm39)	K179R	probably damaging	Het
Arhgap10	C	T	8: 77,977,583 (GRCm39)	G776R	probably damaging	Het
Arhgap35	A	C	7: 16,296,491 (GRCm39)	F858C	probably damaging	Het
Ash1l	C	T	3: 88,984,381 (GRCm39)	R2957W	probably damaging	Het
Atp6ap1l	G	T	13: 91,031,848 (GRCm39)	A278E	probably damaging	Het
Btn1a1	A	T	13: 23,643,521 (GRCm39)	D309E	probably damaging	Het
Casd1	C	T	6: 4,624,211 (GRCm39)	R335C	probably benign	Het
Ceacam3	A	G	7: 16,885,487 (GRCm39)	Q30R		Het
Cfap20	C	T	8: 96,148,795 (GRCm39)	C119Y	probably damaging	Het
Crot	A	G	5: 9,037,878 (GRCm39)	V146A	probably damaging	Het
Cx3cl1	A	G	8: 95,506,591 (GRCm39)	S199G	probably damaging	Het
Ddhd1	A	T	14: 45,895,263 (GRCm39)	L69Q	probably damaging	Het
Dnah12	T	C	14: 26,583,689 (GRCm39)	F3195L	probably damaging	Het
Dpy19l3	A	T	7: 35,408,055 (GRCm39)	D450E	probably benign	Het
Ercc3	A	T	18: 32,390,325 (GRCm39)	N538I	probably damaging	Het
Fzd1	A	T	5: 4,806,145 (GRCm39)	V479E	probably benign	Het
Gart	T	C	16: 91,425,351 (GRCm39)	Y638C	possibly damaging	Het
Gm2381	C	A	7: 42,469,888 (GRCm39)	V79L	probably benign	Het
Gucy1b1	T	C	3: 81,950,469 (GRCm39)	Y312C	probably damaging	Het
Hecw1	T	A	13: 14,609,045 (GRCm39)	M1L	probably benign	Het
Ip6k2	A	G	9: 108,673,930 (GRCm39)	I99V	unknown	Het
Itgb5	C	T	16: 33,761,013 (GRCm39)	T616I	probably benign	Het
Kmt2c	A	C	5: 25,505,360 (GRCm39)	V3316G	possibly damaging	Het
Lama3	C	T	18: 12,601,346 (GRCm39)	R1008C	probably damaging	Het
Lin54	T	C	5: 100,633,159 (GRCm39)	D175G	possibly damaging	Het
Lrp2	A	G	2: 69,318,395 (GRCm39)	S2019P	probably damaging	Het
Lrrc9	A	G	12: 72,513,726 (GRCm39)	E528G	probably benign	Het
Lyz3	T	C	10: 117,073,647 (GRCm39)	N62S	probably benign	Het
Mto1	A	G	9: 78,364,565 (GRCm39)	H299R	probably damaging	Het
Myo19	G	A	11: 84,796,439 (GRCm39)	G684R	probably benign	Het
Or14c45	A	C	7: 86,176,322 (GRCm39)	D119A	probably damaging	Het
Or7g32	A	T	9: 19,408,145 (GRCm39)	M34L	probably benign	Het
Pcdh18	C	A	3: 49,709,143 (GRCm39)	C724F	probably benign	Het
Pcdha3	A	T	18: 37,080,165 (GRCm39)	K302N	probably benign	Het
Pcdhgb2	T	C	18: 37,825,300 (GRCm39)	F764L	possibly damaging	Het
Pik3cb	C	A	9: 98,975,143 (GRCm39)	G246W	probably damaging	Het
Polr2m	A	T	9: 71,390,626 (GRCm39)	I192N	probably damaging	Het
Polr2m	G	T	9: 71,390,815 (GRCm39)	T129K	probably benign	Het
Pramel48	A	G	5: 95,630,680 (GRCm39)	I186V	possibly damaging	Het
Ptpn4	C	T	1: 119,619,475 (GRCm39)		probably null	Het
Scgb1b19	A	T	7: 32,986,940 (GRCm39)	K30N	possibly damaging	Het
Shpk	A	G	11: 73,104,315 (GRCm39)	T155A	probably damaging	Het
Slit3	T	C	11: 35,461,546 (GRCm39)	Y261H	probably damaging	Het
Sox13	A	G	1: 133,313,243 (GRCm39)	F438L	possibly damaging	Het
Sphk1	A	T	11: 116,425,907 (GRCm39)	E61D	probably benign	Het
St18	A	G	1: 6,873,243 (GRCm39)	D326G	probably damaging	Het
Syde1	G	A	10: 78,422,032 (GRCm39)	Q566*	probably null	Het
Tacc2	A	G	7: 130,330,807 (GRCm39)	T254A	probably benign	Het
Tbc1d1	A	G	5: 64,330,827 (GRCm39)	E2G	probably damaging	Het
Trim5	A	T	7: 103,926,017 (GRCm39)	N181K	probably damaging	Het
Trpc7	A	T	13: 56,931,509 (GRCm39)	I730K	probably benign	Het
Uqcrc1	T	A	9: 108,776,926 (GRCm39)	M377K	probably benign	Het
Vmn2r4	T	C	3: 64,305,898 (GRCm39)	N508S	probably benign	Het
Vmp1	A	T	11: 86,477,402 (GRCm39)	S333T	probably benign	Het
Vps50	T	A	6: 3,578,854 (GRCm39)	M639K	possibly damaging	Het
Zfp874b	A	T	13: 67,622,622 (GRCm39)	C225*	probably null	Het

Other mutations in Cntrl

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00392:Cntrl	APN	2	35,027,826 (GRCm39)	splice site	probably benign
IGL00478:Cntrl	APN	2	35,050,613 (GRCm39)	missense	probably damaging	0.98
IGL01460:Cntrl	APN	2	35,055,856 (GRCm39)	missense	probably benign	0.04
IGL01556:Cntrl	APN	2	35,063,071 (GRCm39)	missense	probably benign	0.19
IGL02155:Cntrl	APN	2	35,050,250 (GRCm39)	splice site	probably benign
IGL02419:Cntrl	APN	2	35,024,055 (GRCm39)	missense	probably damaging	0.97
PIT4480001:Cntrl	UTSW	2	35,045,440 (GRCm39)	missense	probably damaging	0.96
R0179:Cntrl	UTSW	2	35,057,871 (GRCm39)	missense	probably benign	0.00
R0276:Cntrl	UTSW	2	35,041,744 (GRCm39)	missense	possibly damaging	0.62
R0471:Cntrl	UTSW	2	35,017,392 (GRCm39)	missense	probably benign	0.41
R0755:Cntrl	UTSW	2	35,035,151 (GRCm39)	missense	probably damaging	1.00
R0763:Cntrl	UTSW	2	35,061,078 (GRCm39)	missense	probably benign
R0781:Cntrl	UTSW	2	35,050,639 (GRCm39)	missense	possibly damaging	0.66
R0791:Cntrl	UTSW	2	35,045,291 (GRCm39)	missense	possibly damaging	0.83
R0792:Cntrl	UTSW	2	35,045,291 (GRCm39)	missense	possibly damaging	0.83
R0801:Cntrl	UTSW	2	35,065,107 (GRCm39)	splice site	probably benign
R1067:Cntrl	UTSW	2	35,039,034 (GRCm39)	unclassified	probably benign
R1110:Cntrl	UTSW	2	35,050,639 (GRCm39)	missense	possibly damaging	0.66
R1117:Cntrl	UTSW	2	35,017,985 (GRCm39)	missense	probably damaging	1.00
R1457:Cntrl	UTSW	2	35,012,768 (GRCm39)	missense	probably benign	0.00
R1472:Cntrl	UTSW	2	35,059,329 (GRCm39)	critical splice donor site	probably null
R1522:Cntrl	UTSW	2	35,045,291 (GRCm39)	missense	possibly damaging	0.83
R1702:Cntrl	UTSW	2	35,061,848 (GRCm39)	critical splice acceptor site	probably null
R1762:Cntrl	UTSW	2	35,012,818 (GRCm39)	frame shift	probably null
R1785:Cntrl	UTSW	2	35,012,818 (GRCm39)	frame shift	probably null
R1786:Cntrl	UTSW	2	35,012,818 (GRCm39)	frame shift	probably null
R1812:Cntrl	UTSW	2	35,039,481 (GRCm39)	missense	probably damaging	0.97
R1854:Cntrl	UTSW	2	35,012,696 (GRCm39)	missense	probably damaging	1.00
R1863:Cntrl	UTSW	2	35,008,131 (GRCm39)	missense	possibly damaging	0.93
R1868:Cntrl	UTSW	2	35,019,827 (GRCm39)	missense	probably benign	0.03
R1914:Cntrl	UTSW	2	35,052,873 (GRCm39)	missense	probably benign	0.00
R1915:Cntrl	UTSW	2	35,052,873 (GRCm39)	missense	probably benign	0.00
R2049:Cntrl	UTSW	2	35,012,818 (GRCm39)	frame shift	probably null
R2118:Cntrl	UTSW	2	35,051,977 (GRCm39)	missense	probably benign	0.31
R2140:Cntrl	UTSW	2	35,012,818 (GRCm39)	frame shift	probably null
R2142:Cntrl	UTSW	2	35,012,818 (GRCm39)	frame shift	probably null
R2203:Cntrl	UTSW	2	35,033,749 (GRCm39)	missense	possibly damaging	0.84
R2300:Cntrl	UTSW	2	35,017,525 (GRCm39)	missense	probably benign	0.00
R2349:Cntrl	UTSW	2	35,066,263 (GRCm39)	missense	probably benign	0.18
R2374:Cntrl	UTSW	2	35,043,288 (GRCm39)	missense	possibly damaging	0.46
R3429:Cntrl	UTSW	2	35,035,112 (GRCm39)	missense	probably damaging	1.00
R3890:Cntrl	UTSW	2	35,060,492 (GRCm39)	missense	probably benign	0.02
R3911:Cntrl	UTSW	2	35,010,061 (GRCm39)	missense	probably damaging	1.00
R3922:Cntrl	UTSW	2	35,019,751 (GRCm39)	missense	probably damaging	0.98
R4081:Cntrl	UTSW	2	35,065,137 (GRCm39)	missense	probably damaging	1.00
R4081:Cntrl	UTSW	2	35,051,938 (GRCm39)	splice site	probably benign
R4516:Cntrl	UTSW	2	35,017,993 (GRCm39)	missense	probably benign	0.00
R4518:Cntrl	UTSW	2	35,038,986 (GRCm39)	missense	probably damaging	1.00
R4519:Cntrl	UTSW	2	35,063,123 (GRCm39)	missense	probably damaging	1.00
R4646:Cntrl	UTSW	2	35,039,473 (GRCm39)	missense	probably damaging	0.99
R4753:Cntrl	UTSW	2	35,043,451 (GRCm39)	missense	possibly damaging	0.90
R4763:Cntrl	UTSW	2	35,065,563 (GRCm39)	missense	probably damaging	1.00
R4916:Cntrl	UTSW	2	35,055,694 (GRCm39)	missense	probably benign	0.42
R5168:Cntrl	UTSW	2	35,047,667 (GRCm39)	missense	probably damaging	1.00
R5291:Cntrl	UTSW	2	35,024,072 (GRCm39)	missense	probably damaging	1.00
R5356:Cntrl	UTSW	2	35,038,911 (GRCm39)	nonsense	probably null
R5774:Cntrl	UTSW	2	35,052,873 (GRCm39)	missense	probably benign	0.15
R5947:Cntrl	UTSW	2	35,006,691 (GRCm39)	missense	probably damaging	1.00
R6144:Cntrl	UTSW	2	35,055,745 (GRCm39)	missense	possibly damaging	0.93
R6147:Cntrl	UTSW	2	35,055,745 (GRCm39)	missense	possibly damaging	0.93
R6214:Cntrl	UTSW	2	35,019,646 (GRCm39)	missense	probably benign	0.10
R6267:Cntrl	UTSW	2	35,019,805 (GRCm39)	missense	probably damaging	1.00
R6332:Cntrl	UTSW	2	35,018,036 (GRCm39)	missense	possibly damaging	0.78
R6445:Cntrl	UTSW	2	35,052,860 (GRCm39)	missense	probably benign	0.05
R6487:Cntrl	UTSW	2	35,012,694 (GRCm39)	missense	possibly damaging	0.89
R6497:Cntrl	UTSW	2	35,025,584 (GRCm39)	missense	possibly damaging	0.66
R6782:Cntrl	UTSW	2	35,060,658 (GRCm39)	missense	possibly damaging	0.75
R6815:Cntrl	UTSW	2	35,039,503 (GRCm39)	missense	probably damaging	1.00
R6853:Cntrl	UTSW	2	35,019,833 (GRCm39)	missense	possibly damaging	0.87
R6858:Cntrl	UTSW	2	35,052,107 (GRCm39)	critical splice donor site	probably null
R6965:Cntrl	UTSW	2	35,052,845 (GRCm39)	missense	probably benign	0.20
R6970:Cntrl	UTSW	2	35,008,149 (GRCm39)	missense	probably benign
R7085:Cntrl	UTSW	2	35,055,804 (GRCm39)	missense	probably benign	0.00
R7213:Cntrl	UTSW	2	35,025,692 (GRCm39)	missense	possibly damaging	0.95
R7221:Cntrl	UTSW	2	35,041,869 (GRCm39)	missense	possibly damaging	0.46
R7389:Cntrl	UTSW	2	35,017,529 (GRCm39)	missense	probably benign	0.01
R7414:Cntrl	UTSW	2	35,055,479 (GRCm39)	missense	probably benign	0.02
R7427:Cntrl	UTSW	2	35,060,546 (GRCm39)	missense	probably benign	0.00
R7428:Cntrl	UTSW	2	35,060,546 (GRCm39)	missense	probably benign	0.00
R7453:Cntrl	UTSW	2	35,045,421 (GRCm39)	missense	possibly damaging	0.89
R7747:Cntrl	UTSW	2	35,006,810 (GRCm39)	missense	probably damaging	1.00
R7753:Cntrl	UTSW	2	35,001,691 (GRCm39)	missense	probably damaging	1.00
R7811:Cntrl	UTSW	2	35,052,873 (GRCm39)	missense	probably benign	0.00
R7882:Cntrl	UTSW	2	35,060,592 (GRCm39)	missense	probably benign	0.41
R7919:Cntrl	UTSW	2	35,017,413 (GRCm39)	missense	probably benign
R8314:Cntrl	UTSW	2	35,065,155 (GRCm39)	missense	probably benign	0.00
R8332:Cntrl	UTSW	2	35,016,037 (GRCm39)	missense	probably damaging	1.00
R8681:Cntrl	UTSW	2	35,038,600 (GRCm39)	missense	probably damaging	1.00
R8698:Cntrl	UTSW	2	35,023,974 (GRCm39)	missense	probably damaging	0.98
R8717:Cntrl	UTSW	2	35,003,351 (GRCm39)	missense	probably benign	0.40
R8960:Cntrl	UTSW	2	35,052,053 (GRCm39)	missense	possibly damaging	0.89
R9036:Cntrl	UTSW	2	35,016,071 (GRCm39)	missense	probably damaging	1.00
R9617:Cntrl	UTSW	2	35,035,077 (GRCm39)	missense	probably benign	0.00
R9621:Cntrl	UTSW	2	35,050,278 (GRCm39)	missense	probably damaging	0.96
RF007:Cntrl	UTSW	2	35,060,512 (GRCm39)	missense	probably benign
RF016:Cntrl	UTSW	2	35,009,998 (GRCm39)	missense	probably benign
RF017:Cntrl	UTSW	2	35,065,201 (GRCm39)	missense	probably damaging	0.96
X0024:Cntrl	UTSW	2	35,037,308 (GRCm39)	missense	probably damaging	1.00
X0026:Cntrl	UTSW	2	35,039,528 (GRCm39)	missense	probably damaging	1.00
X0027:Cntrl	UTSW	2	35,055,694 (GRCm39)	missense	probably benign	0.08
X0027:Cntrl	UTSW	2	35,047,780 (GRCm39)	missense	probably damaging	1.00
X0028:Cntrl	UTSW	2	35,037,356 (GRCm39)	missense	possibly damaging	0.83

Predicted Primers

PCR Primer
(F):5'- GAGTCGGGGCATTGTAACTG -3'
(R):5'- GCTCTAGTTTCTCCTGAGTGAGAG -3'

Sequencing Primer
(F):5'- CTGGAACTCACTTTGTAGACCAGG -3'
(R):5'- TTCTCCTGAGTGAGAGAGAAGATTC -3'

Posted On

2019-05-15