Mutagenetix > Incidental Mutation

Incidental Mutation 'R7153:Tmem225'

554263

Institutional Source

Beutler Lab

Gene Symbol

Tmem225

Ensembl Gene

ENSMUSG00000040541

Gene Name

transmembrane protein 225

Synonyms

MMRRC Submission

045255-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.052) question?

Stock #

R7153 (G1)

Quality Score

225.009

Status

Validated

Chromosome

Chromosomal Location

40148122-40150880 bp(+) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

T to C at 40148368 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Phenylalanine to Leucine at position 15 (F15L)

Ref Sequence

ENSEMBL: ENSMUSP00000036816 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000046333]

AlphaFold

Q9D9S2

Predicted Effect

probably benign
Transcript: ENSMUST00000046333
AA Change: F15L

PolyPhen 2 Score 0.010 (Sensitivity: 0.96; Specificity: 0.77)

SMART Domains

Protein: ENSMUSP00000036816
Gene: ENSMUSG00000040541
AA Change: F15L

Domain	Start	End	E-Value	Type
transmembrane domain	12	34	N/A	INTRINSIC
transmembrane domain	68	90	N/A	INTRINSIC
transmembrane domain	97	119	N/A	INTRINSIC
transmembrane domain	139	161	N/A	INTRINSIC

Coding Region Coverage

1x: 100.0%
3x: 99.9%
10x: 99.6%
20x: 98.8%

Validation Efficiency

100% (75/75)

Allele List at MGI

Other mutations in this stock

Total: 75 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Ano2	A	G	6: 125,992,943	T741A	possibly damaging	Het
Aox4	T	C	1: 58,250,219	M767T	probably damaging	Het
Arhgap39	C	A	15: 76,765,491	S27I	probably benign	Het
AU018091	T	A	7: 3,159,513	M296L	probably benign	Het
Axin1	A	G	17: 26,187,968	T512A	probably benign	Het
B4galnt3	A	T	6: 120,214,968	D602E	probably benign	Het
Bcl6	G	A	16: 23,966,226	R675*	probably null	Het
Bsnd	A	T	4: 106,492,033	D3E	probably benign	Het
Btbd18	C	T	2: 84,666,428	R137*	probably null	Het
C2cd5	A	G	6: 143,019,409	S871P	probably benign	Het
Cobl	G	T	11: 12,254,128	P858Q	probably damaging	Het
Col6a1	C	T	10: 76,710,341		probably null	Het
Crb2	A	T	2: 37,787,408	H304L	probably benign	Het
Crybg1	T	A	10: 43,964,666	Y1812F	possibly damaging	Het
Ddx60	A	T	8: 61,988,108	K1070N	possibly damaging	Het
Defb22	T	C	2: 152,485,920	K115R	unknown	Het
Dhx8	C	T	11: 101,740,175		probably null	Het
Dnah5	T	A	15: 28,365,522		probably null	Het
Dnah7b	T	A	1: 46,126,804	F543Y	probably benign	Het
Ehhadh	A	G	16: 21,766,321	F270S	probably damaging	Het
Esyt2	A	G	12: 116,346,508	M397V	probably benign	Het
Fanca	A	T	8: 123,316,425	L74H	probably damaging	Het
Fbxl17	C	A	17: 63,060,351	V676F	probably benign	Het
Fndc1	A	G	17: 7,801,645	V102A	probably damaging	Het
Gpalpp1	T	C	14: 76,095,011	Y273C	probably damaging	Het
Gpatch8	TTCCTCCTCCTCCTCTTCCTCCTCCTC	TTCCTCCTCCTCCTCCTCTTCCTCCTCCTC	11: 102,480,188		probably benign	Het
Grik2	T	C	10: 49,535,367	H225R	probably benign	Het
Helz2	A	T	2: 181,231,285	S2411T	probably benign	Het
Hnmt	C	T	2: 24,014,341	A103T	probably damaging	Het
Ighv15-2	G	T	12: 114,564,590	Y114*	probably null	Het
Irak2	A	G	6: 113,678,709	H357R	probably benign	Het
Kcp	T	C	6: 29,499,015	E320G	probably damaging	Het
Kpna4	A	G	3: 69,089,798	L352S	probably damaging	Het
Krt36	G	A	11: 100,105,146	Q151*	probably null	Het
Krt77	G	A	15: 101,865,496	T241M	probably benign	Het
Lca5l	T	A	16: 96,173,809	N305I	probably damaging	Het
Lefty1	T	A	1: 180,937,767	L300Q	probably benign	Het
Mertk	A	G	2: 128,736,649	Y185C	probably damaging	Het
Mmp14	T	C	14: 54,436,251	I124T	possibly damaging	Het
Nlrc4	T	C	17: 74,447,103	E95G	possibly damaging	Het
Npc1	A	G	18: 12,213,291	F283L	possibly damaging	Het
Olfr1180	A	C	2: 88,412,118	L180W	probably damaging	Het
Olfr1282	A	T	2: 111,335,901	M59K	probably damaging	Het
Pcdha11	T	C	18: 37,011,225	V123A	probably damaging	Het
Pcdhga2	T	C	18: 37,669,208	V35A	probably damaging	Het
Pgd	G	T	4: 149,161,678	T94K	probably benign	Het
Pik3c2a	T	C	7: 116,342,252	N1593S	probably damaging	Het
Pkd1l3	GACACCTGCATCCAGCAGCCCAACAAACATGACATCAGACACACCTGCATCCAGCAGCCCAACAAACATGACATCAGACACACCTGCATCCAGCAGCCCAACAAACATGACATCAGACACACCTGCATCCAGCAGCCCA	GACACCTGCATCCAGCAGCCCAACAAACATGACATCAGACACACCTGCATCCAGCAGCCCAACAAACATGACATCAGACACACCTGCATCCAGCAGCCCA	8: 109,624,195		probably benign	Het
Plcb3	A	G	19: 6,958,084		probably null	Het
Prkd3	C	T	17: 78,966,355	D491N	probably benign	Het
Ptprf	T	C	4: 118,231,543	T688A	probably damaging	Het
Pycr2	T	A	1: 180,906,682	L210M	probably damaging	Het
Rpl36	T	C	17: 56,614,137	I81T	probably benign	Het
Rraga	C	T	4: 86,576,016	A33V	probably damaging	Het
Slc29a1	G	A	17: 45,585,762	A429V	probably damaging	Het
Stab1	T	G	14: 31,160,584	E432A	probably benign	Het
Synj1	C	T	16: 90,948,090	G1189R	probably benign	Het
Synpo2	A	G	3: 123,112,404	*1088Q	probably null	Het
Tbc1d21	T	C	9: 58,363,093	D133G	probably damaging	Het
Tenm2	A	G	11: 36,024,182	V2176A	probably damaging	Het
Tff1	A	G	17: 31,162,798	I35T	probably benign	Het
Thnsl1	T	C	2: 21,212,953	V506A	possibly damaging	Het
Timm10b	A	G	7: 105,640,880	Q50R	unknown	Het
Tjp2	A	T	19: 24,101,981	N843K	probably benign	Het
Tle1	A	T	4: 72,139,061	F101I	probably benign	Het
Trav12-3	T	C	14: 53,622,161	L88P	probably benign	Het
Ttc7b	A	T	12: 100,355,034	W613R	probably damaging	Het
Ttn	T	A	2: 76,778,504	M17723L	probably benign	Het
Ttn	T	C	2: 76,945,316	D1840G	unknown	Het
Tubgcp2	T	C	7: 140,001,036	H668R	probably benign	Het
Ush2a	C	A	1: 188,728,484	N2647K	possibly damaging	Het
Vmn1r14	C	T	6: 57,233,866	S143F	probably benign	Het
Vmn2r70	A	G	7: 85,565,054	S297P	probably damaging	Het
Ywhab	T	C	2: 164,014,060	F119L	probably damaging	Het
Zmym2	T	A	14: 56,950,202	D1108E	probably benign	Het

Other mutations in Tmem225

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01833:Tmem225	APN	9	40148429	missense	probably damaging	0.98
IGL03094:Tmem225	APN	9	40148386	missense	possibly damaging	0.46
R0599:Tmem225	UTSW	9	40149747	missense	possibly damaging	0.46
R1134:Tmem225	UTSW	9	40149847	missense	possibly damaging	0.92
R2842:Tmem225	UTSW	9	40149801	missense	probably damaging	0.99
R4556:Tmem225	UTSW	9	40149466	missense	probably damaging	0.96
R5024:Tmem225	UTSW	9	40149343	missense	probably benign	0.01
R5526:Tmem225	UTSW	9	40150706	missense	possibly damaging	0.92
R5540:Tmem225	UTSW	9	40149385	missense	probably damaging	0.99
R5597:Tmem225	UTSW	9	40149430	missense	possibly damaging	0.94
R6267:Tmem225	UTSW	9	40148435	missense	probably damaging	0.97
R6314:Tmem225	UTSW	9	40150720	missense	probably benign
R8031:Tmem225	UTSW	9	40149393	missense	possibly damaging	0.46
R8245:Tmem225	UTSW	9	40150659	missense	probably damaging	0.99
X0064:Tmem225	UTSW	9	40149786	missense	possibly damaging	0.85

Predicted Primers

PCR Primer
(F):5'- CTGATGCCTCTGCCAACATC -3'
(R):5'- GAGAAAGTTGCTCCCAGGAATAATG -3'

Sequencing Primer
(F):5'- TCTCTGCAGTCAGTGAACATCCAG -3'
(R):5'- TGGATAAGTTGATCAGATCAGCC -3'

Posted On

2019-05-15