Mutagenetix > Incidental Mutation

Incidental Mutation 'PIT4280001:Gpr35'

554390

Institutional Source

Beutler Lab

Gene Symbol

Gpr35

Ensembl Gene

ENSMUSG00000026271

Gene Name

G protein-coupled receptor 35

Synonyms

Accession Numbers

Essential gene?

Non essential (E-score: 0.000) question?

Stock #

PIT4280001 (G1)

Quality Score

225.009

Status

Not validated

Chromosome

Chromosomal Location

92878587-92914113 bp(+) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

T to C at 92910356 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Tyrosine to Histidine at position 23 (Y23H)

Ref Sequence

ENSEMBL: ENSMUSP00000070832 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000027489] [ENSMUST00000064480] [ENSMUST00000169198] [ENSMUST00000185421] [ENSMUST00000186298] [ENSMUST00000189697]

AlphaFold

Q9ES90

Predicted Effect

probably benign
Transcript: ENSMUST00000027489

SMART Domains

Protein: ENSMUSP00000027489
Gene: ENSMUSG00000026271

Domain	Start	End	E-Value	Type
Pfam:7TM_GPCR_Srsx	28	289	3.5e-7	PFAM
Pfam:7tm_1	34	275	1.8e-34	PFAM

Predicted Effect

probably damaging
Transcript: ENSMUST00000064480
AA Change: Y23H

PolyPhen 2 Score 0.999 (Sensitivity: 0.14; Specificity: 0.99)

SMART Domains

Protein: ENSMUSP00000070832
Gene: ENSMUSG00000026271
AA Change: Y23H

Domain	Start	End	E-Value	Type
Pfam:7TM_GPCR_Srsx	28	289	3.5e-7	PFAM
Pfam:7tm_1	34	275	3.6e-42	PFAM

Predicted Effect

probably damaging
Transcript: ENSMUST00000169198
AA Change: Y23H

PolyPhen 2 Score 0.999 (Sensitivity: 0.14; Specificity: 0.99)

SMART Domains

Protein: ENSMUSP00000126914
Gene: ENSMUSG00000026271
AA Change: Y23H

Domain	Start	End	E-Value	Type
Pfam:7TM_GPCR_Srsx	28	289	3.5e-7	PFAM
Pfam:7tm_1	34	275	3.6e-42	PFAM

Predicted Effect

probably damaging
Transcript: ENSMUST00000185421
AA Change: Y23H

PolyPhen 2 Score 0.999 (Sensitivity: 0.14; Specificity: 0.99)

Predicted Effect

probably damaging
Transcript: ENSMUST00000186298
AA Change: Y23H

PolyPhen 2 Score 0.999 (Sensitivity: 0.14; Specificity: 0.99)

SMART Domains

Protein: ENSMUSP00000139648
Gene: ENSMUSG00000026271
AA Change: Y23H

Domain	Start	End	E-Value	Type
Pfam:7TM_GPCR_Srsx	28	289	3.5e-7	PFAM
Pfam:7tm_1	34	275	3.6e-42	PFAM

Predicted Effect

probably damaging
Transcript: ENSMUST00000189697
AA Change: Y23H

PolyPhen 2 Score 0.999 (Sensitivity: 0.14; Specificity: 0.99)

SMART Domains

Protein: ENSMUSP00000139787
Gene: ENSMUSG00000026271
AA Change: Y23H

Domain	Start	End	E-Value	Type
Pfam:7TM_GPCR_Srsx	28	147	5.7e-6	PFAM
Pfam:7tm_1	34	147	1.4e-24	PFAM

Coding Region Coverage

1x: 93.1%
3x: 90.9%
10x: 85.5%
20x: 73.2%

Validation Efficiency

Allele List at MGI

Other mutations in this stock

Total: 52 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
1700020L24Rik	A	G	11: 83,331,660 (GRCm39)	D161G	probably damaging	Het
Acer2	C	T	4: 86,805,320 (GRCm39)	L95F	probably damaging	Het
Adam15	C	T	3: 89,251,285 (GRCm39)		probably null	Het
Aoc1l3	A	T	6: 48,964,054 (GRCm39)	M21L	probably benign	Het
Bmi1	T	A	2: 18,687,820 (GRCm39)	Y98*	probably null	Het
Cdon	T	C	9: 35,398,231 (GRCm39)	C983R	probably damaging	Het
Cfap91	C	T	16: 38,153,135 (GRCm39)	V160I	probably benign	Het
Col12a1	T	C	9: 79,585,387 (GRCm39)	R1297G	probably damaging	Het
Cpd	A	T	11: 76,681,850 (GRCm39)	M1132K	probably benign	Het
Dnah17	T	A	11: 117,989,408 (GRCm39)	R1267W	possibly damaging	Het
Dnah9	G	A	11: 65,895,839 (GRCm39)	A2512V	probably benign	Het
Eri3	G	A	4: 117,439,831 (GRCm39)	G175D	probably damaging	Het
Fbxo9	C	A	9: 77,994,793 (GRCm39)	W244L	probably damaging	Het
Fgfr3	A	T	5: 33,889,576 (GRCm39)	H343L	probably benign	Het
Fmnl2	G	A	2: 53,008,208 (GRCm39)	A803T	unknown	Het
Fmnl3	A	G	15: 99,219,134 (GRCm39)		probably null	Het
Fth1	C	A	19: 9,961,973 (GRCm39)	A104E	probably damaging	Het
Gcm1	T	C	9: 77,966,915 (GRCm39)	Y45H	probably damaging	Het
Gm5157	C	G	7: 20,919,007 (GRCm39)	G179R	probably damaging	Het
Gpr179	A	G	11: 97,234,941 (GRCm39)	I463T	probably damaging	Het
Inpp4b	C	T	8: 82,761,046 (GRCm39)	H647Y	probably benign	Het
Kif6	A	T	17: 50,062,148 (GRCm39)	K436M	probably benign	Het
Lacc1	T	A	14: 77,272,517 (GRCm39)	Q93L	probably damaging	Het
Lamc1	T	C	1: 153,119,217 (GRCm39)	R801G	probably damaging	Het
Lrp2bp	T	G	8: 46,476,048 (GRCm39)	V263G	probably damaging	Het
Magi3	T	A	3: 103,961,668 (GRCm39)	K453N	probably damaging	Het
Mfsd6	T	A	1: 52,700,039 (GRCm39)	Y703F	probably benign	Het
Mms22l	A	G	4: 24,581,149 (GRCm39)	T820A	probably benign	Het
Ndufa12	T	C	10: 94,034,994 (GRCm39)		probably null	Het
Nlrp12	A	T	7: 3,290,063 (GRCm39)	C150S	possibly damaging	Het
Noc4l	G	A	5: 110,799,305 (GRCm39)	T159I	probably benign	Het
Olfml2b	T	A	1: 170,475,305 (GRCm39)	C77S	probably damaging	Het
Or4c12	A	T	2: 89,774,087 (GRCm39)	I124N	probably damaging	Het
Or9a4	C	A	6: 40,548,650 (GRCm39)	T110K	probably damaging	Het
Pdzd2	A	G	15: 12,399,374 (GRCm39)	V784A	probably damaging	Het
Pip5kl1	A	T	2: 32,473,470 (GRCm39)	Y369F	probably benign	Het
Pkdrej	A	T	15: 85,704,136 (GRCm39)	I600N	probably benign	Het
Psg19	T	C	7: 18,530,831 (GRCm39)	I108V	probably damaging	Het
Pxdn	G	A	12: 30,045,327 (GRCm39)	V539M	probably damaging	Het
Resf1	T	G	6: 149,227,023 (GRCm39)	I23S	probably benign	Het
Rsbn1l	T	A	5: 21,124,653 (GRCm39)	H383L	probably damaging	Het
Scamp2	A	T	9: 57,488,076 (GRCm39)	N118I	probably damaging	Het
Scn2a	T	C	2: 65,546,074 (GRCm39)	V879A	probably damaging	Het
Scn8a	G	A	15: 100,855,370 (GRCm39)	E172K	probably damaging	Het
Slc16a6	C	T	11: 109,349,419 (GRCm39)	C214Y	possibly damaging	Het
Stag1	A	G	9: 100,824,769 (GRCm39)	T922A	possibly damaging	Het
Tarbp1	T	C	8: 127,157,586 (GRCm39)	H1307R	probably damaging	Het
Tut1	T	G	19: 8,936,626 (GRCm39)	V150G	probably benign	Het
Ubr2	G	A	17: 47,255,789 (GRCm39)	R1371W	probably damaging	Het
Vmn2r124	A	G	17: 18,283,487 (GRCm39)	N394D	probably benign	Het
Vmn2r63	T	C	7: 42,553,409 (GRCm39)	T616A	probably damaging	Het
Zp3	G	A	5: 136,013,318 (GRCm39)	V217M	possibly damaging	Het

Other mutations in Gpr35

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00509:Gpr35	APN	1	92,910,594 (GRCm39)	missense	probably damaging	0.99
IGL03102:Gpr35	APN	1	92,910,299 (GRCm39)	missense	probably benign	0.00
R0015:Gpr35	UTSW	1	92,910,954 (GRCm39)	missense	probably damaging	1.00
R0412:Gpr35	UTSW	1	92,910,506 (GRCm39)	missense	probably benign	0.01
R1203:Gpr35	UTSW	1	92,910,870 (GRCm39)	missense	probably damaging	0.98
R1515:Gpr35	UTSW	1	92,910,770 (GRCm39)	missense	probably damaging	1.00
R2872:Gpr35	UTSW	1	92,910,848 (GRCm39)	missense	probably benign	0.34
R2872:Gpr35	UTSW	1	92,910,848 (GRCm39)	missense	probably benign	0.34
R3498:Gpr35	UTSW	1	92,911,113 (GRCm39)	missense	probably damaging	1.00
R3499:Gpr35	UTSW	1	92,911,113 (GRCm39)	missense	probably damaging	1.00
R4732:Gpr35	UTSW	1	92,911,107 (GRCm39)	missense	probably damaging	1.00
R4733:Gpr35	UTSW	1	92,911,107 (GRCm39)	missense	probably damaging	1.00
R5270:Gpr35	UTSW	1	92,910,299 (GRCm39)	missense	probably benign	0.00
R5969:Gpr35	UTSW	1	92,910,942 (GRCm39)	missense	probably damaging	1.00
R6769:Gpr35	UTSW	1	92,910,426 (GRCm39)	missense	probably damaging	0.97
R6771:Gpr35	UTSW	1	92,910,426 (GRCm39)	missense	probably damaging	0.97
R7144:Gpr35	UTSW	1	92,910,353 (GRCm39)	missense	probably benign	0.11
R7395:Gpr35	UTSW	1	92,910,929 (GRCm39)	missense	probably damaging	1.00
R8156:Gpr35	UTSW	1	92,910,437 (GRCm39)	missense	probably damaging	1.00
Z1177:Gpr35	UTSW	1	92,910,738 (GRCm39)	missense	probably benign	0.00

Predicted Primers

PCR Primer
(F):5'- ATGCCTGCTCCAGAATTCC -3'
(R):5'- TAGATGCCCTGTGAGAGCTG -3'

Sequencing Primer
(F):5'- AGAATTCCTGCCCAGAGGTG -3'
(R):5'- GTGTCTGAAGAACTATATTTCAGGG -3'

Posted On

2019-06-07