Mutagenetix > Incidental Mutation

Incidental Mutation 'PIT4362001:Gstp2'

554660

Institutional Source

Beutler Lab

Gene Symbol

Gstp2

Ensembl Gene

ENSMUSG00000038155

Gene Name

glutathione S-transferase, pi 2

Synonyms

Gst p-2, GSTpiA, Gst3, Gst-3

Accession Numbers

Essential gene?

Possibly non essential (E-score: 0.428) question?

Stock #

PIT4362001 (G1)

Quality Score

161.009

Status

Not validated

Chromosome

Chromosomal Location

4040285-4042236 bp(-) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

C to A at 4040713 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Aspartic acid to Tyrosine at position 147 (D147Y)

Ref Sequence

ENSEMBL: ENSMUSP00000038931 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000042700] [ENSMUST00000169613]

AlphaFold

P46425

Predicted Effect

possibly damaging
Transcript: ENSMUST00000042700
AA Change: D147Y

PolyPhen 2 Score 0.825 (Sensitivity: 0.84; Specificity: 0.93)

SMART Domains

Protein: ENSMUSP00000038931
Gene: ENSMUSG00000038155
AA Change: D147Y

Domain	Start	End	E-Value	Type
Pfam:GST_N	2	75	4.2e-8	PFAM
Pfam:GST_C	97	188	1.4e-15	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000169613

SMART Domains

Protein: ENSMUSP00000129565
Gene: ENSMUSG00000060803

Domain	Start	End	E-Value	Type
Pfam:GST_N	4	75	2.3e-8	PFAM
Pfam:GST_C	97	188	1.5e-15	PFAM

Coding Region Coverage

1x: 93.4%
3x: 91.2%
10x: 87.0%
20x: 78.3%

Validation Efficiency

MGI Phenotype

PHENOTYPE: Mutant mice with null mutations in both Gstp1 and Gstp2 exhibit an increased susceptibility to DMBA and TPA induced skin papillomas. Male mutant mice exhibit an increased body weight with age. [provided by MGI curators]

Allele List at MGI

Other mutations in this stock

Total: 71 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Abcg1	T	C	17: 31,064,424	S28P	possibly damaging	Het
Akr1c14	T	C	13: 4,079,100	V165A	probably damaging	Het
Aox2	A	T	1: 58,282,680	T44S	probably damaging	Het
Arhgap29	T	A	3: 122,003,212	N482K	probably benign	Het
Arhgef38	T	A	3: 133,160,830	D182V		Het
Atad5	C	T	11: 80,111,567	H1062Y	probably benign	Het
Atp6v0b	A	G	4: 117,885,256	S147P	possibly damaging	Het
Cblb	T	A	16: 52,139,542	Y299*	probably null	Het
Ccdc28b	T	C	4: 129,621,025	N97S	probably benign	Het
Cdh23	A	G	10: 60,465,458	V479A	probably benign	Het
Chuk	A	G	19: 44,098,583		probably null	Het
Cmtr2	G	A	8: 110,222,336	G426D	probably damaging	Het
Cog4	G	A	8: 110,866,672	D472N	probably damaging	Het
Creb3	T	A	4: 43,565,472	L193*	probably null	Het
Cxcr6	A	T	9: 123,810,461	I183F	probably benign	Het
Dbf4	A	G	5: 8,403,664	F253L	probably benign	Het
Dcaf8	T	C	1: 172,172,797	V174A	probably damaging	Het
Ddhd2	T	C	8: 25,735,752	Y526C	probably damaging	Het
Dnal4	A	G	15: 79,763,565	V33A	probably benign	Het
Egfl7	C	T	2: 26,591,040	P188L	probably benign	Het
Ephb3	A	G	16: 21,220,857	E707G	probably damaging	Het
Epn3	C	T	11: 94,496,523	R7H	probably damaging	Het
Fam198b	C	A	3: 79,886,939	S238Y	possibly damaging	Het
Fbp1	T	A	13: 62,867,380	I262F	probably damaging	Het
Fgfbp3	G	A	19: 36,918,688	R177*	probably null	Het
Gbp5	T	C	3: 142,500,710	S52P	probably damaging	Het
Glb1l2	A	T	9: 26,773,981	S282T	probably benign	Het
Glg1	A	T	8: 111,258,799	V133E	possibly damaging	Het
Gm4787	T	G	12: 81,377,175	L736F	probably benign	Het
Gm5565	A	T	5: 146,158,299	S212R	probably benign	Het
Grin2c	T	C	11: 115,249,633	T1220A	probably benign	Het
Grp	T	G	18: 65,886,226	S133A	probably benign	Het
Igkv14-130	T	C	6: 67,791,408	F84L	probably damaging	Het
Inppl1	T	C	7: 101,826,013	R944G	probably benign	Het
Lama2	A	T	10: 27,369,136	N216K	probably damaging	Het
Lrp2	T	A	2: 69,537,538	D210V	probably damaging	Het
Lrrc2	A	T	9: 110,962,540	Q120L	possibly damaging	Het
Lrriq1	A	G	10: 103,071,194	I1555T	probably benign	Het
Map2	G	A	1: 66,412,518	G189D	probably benign	Het
Mdc1	A	G	17: 35,844,469	E12G	possibly damaging	Het
Mdga2	T	C	12: 66,797,768	D152G	possibly damaging	Het
Med23	A	G	10: 24,874,571	M99V	probably benign	Het
Mutyh	G	A	4: 116,817,070	V273M	probably damaging	Het
Neurod2	T	A	11: 98,327,882	Y152F	probably damaging	Het
Olfr1002	A	C	2: 85,647,724	L199R	probably damaging	Het
Olfr1177-ps	C	T	2: 88,344,013	A247T	probably benign	Het
Olfr898	G	T	9: 38,349,198	L32F	probably benign	Het
Pcdh20	G	C	14: 88,467,026	P946R	probably damaging	Het
Pde6b	T	C	5: 108,423,585		probably null	Het
Pdzrn4	A	T	15: 92,769,881	D638V	possibly damaging	Het
Polr1b	A	G	2: 129,109,292	D275G	possibly damaging	Het
Rnf157	T	A	11: 116,360,317	D127V	probably damaging	Het
Rspo4	T	A	2: 151,867,883	C69*	probably null	Het
Scara3	T	C	14: 65,936,402	T63A	probably benign	Het
Scn2a	G	A	2: 65,683,838	E289K	probably benign	Het
Sh3gl2	A	G	4: 85,377,549	T163A	probably benign	Het
Slc2a10	T	A	2: 165,516,293	F446Y	probably damaging	Het
Snapc1	C	T	12: 73,982,495	R351C	probably damaging	Het
Snx6	A	G	12: 54,768,030	Y169H	possibly damaging	Het
Stab2	A	T	10: 86,861,435	C1996*	probably null	Het
Steap4	A	G	5: 7,980,337	T398A	probably benign	Het
Tep1	A	T	14: 50,866,053	L260Q	probably benign	Het
Tspan12	A	T	6: 21,835,464	V70D	possibly damaging	Het
Ttn	G	A	2: 76,739,018	A27177V	probably damaging	Het
Ube3a	T	C	7: 59,276,122	V237A	possibly damaging	Het
Vil1	G	A	1: 74,421,383	R233H	probably damaging	Het
Xrcc5	A	G	1: 72,393,929	T716A	probably benign	Het
Zbp1	A	G	2: 173,216,990	I18T	probably damaging	Het
Zfp292	A	G	4: 34,807,524	V1845A	probably benign	Het
Zfp407	A	T	18: 84,561,268	N573K	possibly damaging	Het
Zfp64	T	C	2: 168,925,815	T626A	probably benign	Het

Other mutations in Gstp2

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL02387:Gstp2	APN	19	4041094	unclassified	probably benign
R0196:Gstp2	UTSW	19	4040514	splice site	probably null
R0545:Gstp2	UTSW	19	4041633	missense	possibly damaging	0.88
R6324:Gstp2	UTSW	19	4040499	missense	probably benign	0.00
R6478:Gstp2	UTSW	19	4040499	missense	probably benign	0.00
R7318:Gstp2	UTSW	19	4041065	missense	probably benign	0.00
R8218:Gstp2	UTSW	19	4041668	missense	probably benign	0.00
R9799:Gstp2	UTSW	19	4041901	critical splice donor site	probably null
Z1177:Gstp2	UTSW	19	4041583	critical splice donor site	probably null

Predicted Primers

PCR Primer
(F):5'- AGGACTTGGTGGATCAGCAG -3'
(R):5'- TGGAATTCATTACAGGGCAGC -3'

Sequencing Primer
(F):5'- TCCAGCAAGTTGTAATCGGC -3'
(R):5'- TTCATTACAGGGCAGCAGGAGTC -3'

Posted On

2019-06-07