Mutagenetix > Incidental Mutation

Incidental Mutation 'PIT4366001:Akap9'

554753

Institutional Source

Beutler Lab

Gene Symbol

Akap9

Ensembl Gene

ENSMUSG00000040407

Gene Name

A kinase (PRKA) anchor protein (yotiao) 9

Synonyms

AKAP450, G1-448-15, 5730481H23Rik, mei2-5, repro12

Accession Numbers

Essential gene?

Possibly non essential (E-score: 0.327) question?

Stock #

PIT4366001 (G1)

Quality Score

225.009

Status

Not validated

Chromosome

Chromosomal Location

3928054-4081310 bp(+) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

T to A at 4046221 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Aspartic acid to Glutamic Acid at position 2365 (D2365E)

Ref Sequence

ENSEMBL: ENSMUSP00000046129 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000044492]

AlphaFold

no structure available at present

Predicted Effect

probably benign
Transcript: ENSMUST00000044492
AA Change: D2365E

PolyPhen 2 Score 0.237 (Sensitivity: 0.91; Specificity: 0.88)

SMART Domains

Protein: ENSMUSP00000046129
Gene: ENSMUSG00000040407
AA Change: D2365E

Domain	Start	End	E-Value	Type
low complexity region	9	18	N/A	INTRINSIC
low complexity region	33	44	N/A	INTRINSIC
low complexity region	98	115	N/A	INTRINSIC
Blast:HPT	126	197	6e-21	BLAST
low complexity region	237	249	N/A	INTRINSIC
low complexity region	297	315	N/A	INTRINSIC
coiled coil region	404	593	N/A	INTRINSIC
coiled coil region	622	756	N/A	INTRINSIC
coiled coil region	777	843	N/A	INTRINSIC
coiled coil region	888	958	N/A	INTRINSIC
low complexity region	982	997	N/A	INTRINSIC
coiled coil region	1037	1065	N/A	INTRINSIC
low complexity region	1233	1246	N/A	INTRINSIC
internal_repeat_2	1247	1312	7.75e-5	PROSPERO
internal_repeat_1	1377	1485	2.63e-5	PROSPERO
coiled coil region	1522	1589	N/A	INTRINSIC
coiled coil region	1789	2107	N/A	INTRINSIC
coiled coil region	2132	2318	N/A	INTRINSIC
internal_repeat_1	2322	2445	2.63e-5	PROSPERO
coiled coil region	2455	2494	N/A	INTRINSIC
low complexity region	2587	2598	N/A	INTRINSIC
low complexity region	2627	2640	N/A	INTRINSIC
internal_repeat_2	2934	2997	7.75e-5	PROSPERO
low complexity region	3000	3016	N/A	INTRINSIC
coiled coil region	3109	3307	N/A	INTRINSIC
coiled coil region	3455	3493	N/A	INTRINSIC
coiled coil region	3521	3556	N/A	INTRINSIC
Pfam:PACT_coil_coil	3576	3657	1.2e-27	PFAM

Coding Region Coverage

1x: 93.0%
3x: 90.7%
10x: 85.5%
20x: 73.8%

Validation Efficiency

MGI Phenotype

PHENOTYPE: Mice homozygous for a chemically induced allele exhibit male infertily with abnormal spermatogenesis and Sertoli maturation. [provided by MGI curators]

Allele List at MGI

Other mutations in this stock

Total: 68 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Aatk	C	A	11: 120,010,960 (GRCm38)	S870I	possibly damaging	Het
Abca13	T	C	11: 9,294,962 (GRCm38)	V2275A	probably benign	Het
Abcb5	T	C	12: 118,936,098 (GRCm38)	K278R	probably damaging	Het
Adcy2	TA	TAA	13: 68,709,990 (GRCm38)		probably benign	Het
Add3	C	T	19: 53,216,867 (GRCm38)	P16L	unknown	Het
Asah1	A	G	8: 41,343,746 (GRCm38)	S300P	possibly damaging	Het
Astn1	A	T	1: 158,597,209 (GRCm38)	H655L	probably benign	Het
Astn1	A	T	1: 158,597,211 (GRCm38)	I656F	probably benign	Het
Bmpr1b	T	A	3: 141,880,463 (GRCm38)	T13S	probably benign	Het
Camsap2	A	G	1: 136,280,317 (GRCm38)	F478L		Het
Card10	G	A	15: 78,787,431 (GRCm38)	S611L	probably benign	Het
Cep68	T	C	11: 20,240,007 (GRCm38)	N335S	probably benign	Het
Chac1	T	C	2: 119,351,505 (GRCm38)	W35R	probably damaging	Het
Cntn6	T	C	6: 104,832,537 (GRCm38)	V511A	probably benign	Het
Cpne9	G	T	6: 113,294,746 (GRCm38)	G356W	probably damaging	Het
Dnah10	C	A	5: 124,775,524 (GRCm38)	T1939K	possibly damaging	Het
Dock10	T	C	1: 80,595,721 (GRCm38)	N239D	probably benign	Het
Dock5	T	C	14: 67,824,674 (GRCm38)	K415R	possibly damaging	Het
Dtd2	C	A	12: 51,999,799 (GRCm38)	D86Y	probably damaging	Het
Efcab9	A	G	11: 32,523,608 (GRCm38)	F127S	probably damaging	Het
Ermp1	T	C	19: 29,628,789 (GRCm38)	H375R	probably benign	Het
Frem2	A	T	3: 53,653,201 (GRCm38)	M1295K	probably damaging	Het
Gbp7	T	G	3: 142,542,951 (GRCm38)	I325R	probably benign	Het
Gpr179	T	C	11: 97,336,851 (GRCm38)	K1493E	probably benign	Het
Gucy2g	T	A	19: 55,237,782 (GRCm38)	E234V	probably null	Het
Itpr1	T	A	6: 108,493,757 (GRCm38)	C2215*	probably null	Het
Ltn1	G	A	16: 87,380,840 (GRCm38)	R1634*	probably null	Het
Lynx1	A	T	15: 74,751,409 (GRCm38)	M58K	possibly damaging	Het
Macc1	T	C	12: 119,446,949 (GRCm38)	V484A	probably benign	Het
Map3k9	C	T	12: 81,772,761 (GRCm38)	V240M	possibly damaging	Het
Marc1	A	G	1: 184,807,186 (GRCm38)	F96S	probably benign	Het
Mars	A	G	10: 127,299,398 (GRCm38)	M608T	possibly damaging	Het
Mc2r	T	C	18: 68,407,755 (GRCm38)	M156V	probably benign	Het
Mchr1	G	T	15: 81,237,216 (GRCm38)	V56L	probably benign	Het
Mlxip	C	T	5: 123,395,110 (GRCm38)	P61S	probably benign	Het
Muc4	A	G	16: 32,754,796 (GRCm38)	T1557A	unknown	Het
Myt1	T	C	2: 181,825,938 (GRCm38)	V1135A	probably damaging	Het
Ndufaf6	A	T	4: 11,073,215 (GRCm38)	S76T	probably benign	Het
Ntrk2	A	G	13: 59,060,335 (GRCm38)	Y665C	probably damaging	Het
Oaz3	T	G	3: 94,433,594 (GRCm38)	R215S	unknown	Het
Olfr1022	T	C	2: 85,868,882 (GRCm38)	C97R	probably damaging	Het
Olfr1031	A	T	2: 85,992,041 (GRCm38)	N75Y	probably damaging	Het
Olfr1133	C	T	2: 87,645,190 (GRCm38)	W311*	probably null	Het
Olfr417	T	C	1: 174,369,090 (GRCm38)	Y58H	probably damaging	Het
Perm1	G	T	4: 156,218,735 (GRCm38)	V579L	probably benign	Het
Phyhipl	A	G	10: 70,568,958 (GRCm38)	V140A	probably benign	Het
Pip5k1c	T	A	10: 81,309,008 (GRCm38)	S228T	probably damaging	Het
Plekhn1	T	G	4: 156,224,811 (GRCm38)	T213P	probably damaging	Het
Pomt2	A	T	12: 87,116,529 (GRCm38)		probably null	Het
Ptcd1	A	G	5: 145,151,335 (GRCm38)	V622A	probably benign	Het
Ptn	T	A	6: 36,741,349 (GRCm38)	H127L	probably benign	Het
Ptprk	A	T	10: 28,586,019 (GRCm38)	M1193L	probably benign	Het
Ptpru	G	A	4: 131,799,712 (GRCm38)	P650S	probably benign	Het
Pum2	T	A	12: 8,733,390 (GRCm38)	L613Q	probably damaging	Het
Rapgef6	A	G	11: 54,691,620 (GRCm38)	T1458A	probably damaging	Het
Sap130	C	T	18: 31,677,409 (GRCm38)	A470V	probably benign	Het
Sertad2	C	A	11: 20,648,116 (GRCm38)	P104Q	probably benign	Het
Sfrp4	A	T	13: 19,630,244 (GRCm38)	M324L	unknown	Het
Sh3bp4	A	G	1: 89,145,434 (GRCm38)	N668S	probably benign	Het
Snx30	C	A	4: 59,894,653 (GRCm38)	D410E	probably benign	Het
Spata31	C	A	13: 64,921,505 (GRCm38)	S489*	probably null	Het
Taok3	T	A	5: 117,227,985 (GRCm38)	M367K	probably benign	Het
Tfap2a	T	C	13: 40,721,374 (GRCm38)	N254D	possibly damaging	Het
Tgtp1	T	G	11: 48,987,040 (GRCm38)	L279F	possibly damaging	Het
Tmpo	A	G	10: 91,163,310 (GRCm38)	F205S	probably damaging	Het
Tmprss5	A	T	9: 49,112,217 (GRCm38)	I218L	probably benign	Het
Tti2	A	G	8: 31,151,196 (GRCm38)	E116G	probably benign	Het
Vmn2r59	A	T	7: 42,045,781 (GRCm38)	N402K	possibly damaging	Het

Other mutations in Akap9

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00478:Akap9	APN	5	4,046,639 (GRCm38)	missense	probably damaging	0.97
IGL00642:Akap9	APN	5	3,960,842 (GRCm38)	missense	probably damaging	0.99
IGL00786:Akap9	APN	5	4,070,522 (GRCm38)	missense	probably damaging	1.00
IGL00788:Akap9	APN	5	4,060,480 (GRCm38)	missense	probably damaging	1.00
IGL00969:Akap9	APN	5	4,001,550 (GRCm38)	missense	probably benign
IGL01014:Akap9	APN	5	3,968,683 (GRCm38)	missense	probably benign	0.41
IGL01302:Akap9	APN	5	3,970,711 (GRCm38)	missense	probably benign	0.27
IGL01610:Akap9	APN	5	4,032,839 (GRCm38)	missense	possibly damaging	0.95
IGL01620:Akap9	APN	5	3,960,218 (GRCm38)	missense	probably benign	0.11
IGL01862:Akap9	APN	5	4,065,856 (GRCm38)	missense	probably damaging	0.99
IGL01862:Akap9	APN	5	3,951,705 (GRCm38)	missense	probably damaging	0.99
IGL02151:Akap9	APN	5	4,032,728 (GRCm38)	nonsense	probably null
IGL02635:Akap9	APN	5	4,070,500 (GRCm38)	missense	possibly damaging	0.59
IGL02858:Akap9	APN	5	4,069,130 (GRCm38)	missense	possibly damaging	0.88
IGL02967:Akap9	APN	5	3,976,164 (GRCm38)	missense	probably benign	0.07
IGL03064:Akap9	APN	5	3,968,755 (GRCm38)	missense	probably damaging	1.00
IGL03289:Akap9	APN	5	4,077,261 (GRCm38)	missense	probably damaging	1.00
Andy	UTSW	5	3,961,764 (GRCm38)	nonsense	probably null
blimey	UTSW	5	4,070,397 (GRCm38)	nonsense	probably null
hoarder	UTSW	5	4,069,089 (GRCm38)	missense	probably benign	0.00
marinarum	UTSW	5	4,013,875 (GRCm38)	nonsense	probably null
miser	UTSW	5	4,046,064 (GRCm38)	missense	probably benign	0.13
naviculus	UTSW	5	3,960,865 (GRCm38)	missense	probably damaging	0.98
thrifty	UTSW	5	3,976,209 (GRCm38)	missense	probably damaging	0.99
wee_one	UTSW	5	4,043,925 (GRCm38)	missense	probably damaging	1.00
FR4449:Akap9	UTSW	5	3,981,214 (GRCm38)	unclassified	probably benign
PIT1430001:Akap9	UTSW	5	4,029,849 (GRCm38)	missense	probably damaging	1.00
R0088:Akap9	UTSW	5	3,961,946 (GRCm38)	missense	probably benign	0.22
R0309:Akap9	UTSW	5	4,069,038 (GRCm38)	missense	probably benign	0.01
R0387:Akap9	UTSW	5	3,951,678 (GRCm38)	splice site	probably benign
R0440:Akap9	UTSW	5	4,064,569 (GRCm38)	missense	probably damaging	0.99
R0441:Akap9	UTSW	5	3,961,714 (GRCm38)	missense	probably benign	0.15
R0491:Akap9	UTSW	5	3,972,851 (GRCm38)	unclassified	probably benign
R0501:Akap9	UTSW	5	3,970,685 (GRCm38)	missense	probably damaging	1.00
R0507:Akap9	UTSW	5	4,069,043 (GRCm38)	missense	probably benign	0.41
R0544:Akap9	UTSW	5	4,069,185 (GRCm38)	missense	probably benign	0.22
R0581:Akap9	UTSW	5	4,050,620 (GRCm38)	missense	probably benign	0.03
R0611:Akap9	UTSW	5	3,954,870 (GRCm38)	missense	probably benign	0.00
R0620:Akap9	UTSW	5	4,064,136 (GRCm38)	missense	probably damaging	0.98
R0639:Akap9	UTSW	5	4,060,318 (GRCm38)	missense	probably damaging	1.00
R0932:Akap9	UTSW	5	4,046,492 (GRCm38)	missense	possibly damaging	0.77
R0944:Akap9	UTSW	5	4,064,742 (GRCm38)	splice site	probably null
R1101:Akap9	UTSW	5	4,046,205 (GRCm38)	missense	probably benign	0.00
R1159:Akap9	UTSW	5	3,960,865 (GRCm38)	missense	probably damaging	0.98
R1170:Akap9	UTSW	5	4,055,671 (GRCm38)	missense	probably benign
R1185:Akap9	UTSW	5	3,948,783 (GRCm38)	missense	probably benign	0.13
R1185:Akap9	UTSW	5	3,948,783 (GRCm38)	missense	probably benign	0.13
R1185:Akap9	UTSW	5	3,948,783 (GRCm38)	missense	probably benign	0.13
R1453:Akap9	UTSW	5	3,975,614 (GRCm38)	splice site	probably null
R1551:Akap9	UTSW	5	4,069,174 (GRCm38)	missense	probably benign	0.02
R1608:Akap9	UTSW	5	3,961,783 (GRCm38)	missense	probably damaging	1.00
R1652:Akap9	UTSW	5	4,077,210 (GRCm38)	missense	probably damaging	1.00
R1659:Akap9	UTSW	5	4,064,633 (GRCm38)	missense	probably damaging	1.00
R1713:Akap9	UTSW	5	4,039,345 (GRCm38)	critical splice donor site	probably null
R1719:Akap9	UTSW	5	3,957,645 (GRCm38)	nonsense	probably null
R1720:Akap9	UTSW	5	3,972,791 (GRCm38)	missense	possibly damaging	0.63
R1757:Akap9	UTSW	5	4,001,667 (GRCm38)	missense	probably benign	0.41
R1872:Akap9	UTSW	5	4,001,406 (GRCm38)	missense	probably damaging	1.00
R1876:Akap9	UTSW	5	3,961,809 (GRCm38)	missense	probably benign	0.28
R1881:Akap9	UTSW	5	4,050,173 (GRCm38)	missense	probably benign
R1950:Akap9	UTSW	5	3,960,677 (GRCm38)	missense	probably damaging	1.00
R1980:Akap9	UTSW	5	3,972,771 (GRCm38)	missense	probably damaging	0.99
R1993:Akap9	UTSW	5	4,038,520 (GRCm38)	splice site	probably null
R2008:Akap9	UTSW	5	3,960,131 (GRCm38)	missense	possibly damaging	0.47
R2020:Akap9	UTSW	5	3,961,967 (GRCm38)	missense	probably damaging	1.00
R2051:Akap9	UTSW	5	3,975,685 (GRCm38)	nonsense	probably null
R2061:Akap9	UTSW	5	3,961,010 (GRCm38)	missense	probably damaging	1.00
R2109:Akap9	UTSW	5	4,044,847 (GRCm38)	missense	possibly damaging	0.47
R2135:Akap9	UTSW	5	4,064,509 (GRCm38)	missense	probably damaging	1.00
R2225:Akap9	UTSW	5	4,077,271 (GRCm38)	missense	probably damaging	0.96
R2232:Akap9	UTSW	5	4,046,603 (GRCm38)	missense	probably damaging	1.00
R2424:Akap9	UTSW	5	4,065,279 (GRCm38)	missense	probably damaging	0.97
R2483:Akap9	UTSW	5	3,976,235 (GRCm38)	missense	possibly damaging	0.65
R2879:Akap9	UTSW	5	3,976,353 (GRCm38)	intron	probably benign
R3622:Akap9	UTSW	5	3,976,235 (GRCm38)	missense	possibly damaging	0.65
R3623:Akap9	UTSW	5	3,976,235 (GRCm38)	missense	possibly damaging	0.65
R3624:Akap9	UTSW	5	3,976,235 (GRCm38)	missense	possibly damaging	0.65
R3722:Akap9	UTSW	5	4,070,351 (GRCm38)	missense	probably damaging	1.00
R3806:Akap9	UTSW	5	3,954,410 (GRCm38)	missense	probably benign	0.00
R3919:Akap9	UTSW	5	3,961,764 (GRCm38)	nonsense	probably null
R4023:Akap9	UTSW	5	3,992,077 (GRCm38)	missense	possibly damaging	0.66
R4093:Akap9	UTSW	5	4,043,996 (GRCm38)	missense	probably damaging	0.99
R4434:Akap9	UTSW	5	4,032,708 (GRCm38)	missense	probably damaging	0.99
R4529:Akap9	UTSW	5	4,043,948 (GRCm38)	missense	probably damaging	1.00
R4530:Akap9	UTSW	5	4,043,948 (GRCm38)	missense	probably damaging	1.00
R4532:Akap9	UTSW	5	4,043,948 (GRCm38)	missense	probably damaging	1.00
R4533:Akap9	UTSW	5	4,043,948 (GRCm38)	missense	probably damaging	1.00
R4585:Akap9	UTSW	5	3,976,151 (GRCm38)	missense	probably benign	0.00
R4586:Akap9	UTSW	5	3,976,151 (GRCm38)	missense	probably benign	0.00
R4655:Akap9	UTSW	5	4,046,403 (GRCm38)	missense	probably benign	0.14
R4676:Akap9	UTSW	5	4,064,515 (GRCm38)	nonsense	probably null
R4676:Akap9	UTSW	5	4,032,774 (GRCm38)	missense	probably damaging	1.00
R4724:Akap9	UTSW	5	4,055,339 (GRCm38)	missense	probably benign
R4731:Akap9	UTSW	5	3,962,266 (GRCm38)	missense	possibly damaging	0.54
R4732:Akap9	UTSW	5	4,013,901 (GRCm38)	missense	probably damaging	0.98
R4733:Akap9	UTSW	5	4,013,901 (GRCm38)	missense	probably damaging	0.98
R4743:Akap9	UTSW	5	3,961,013 (GRCm38)	missense	probably damaging	1.00
R4749:Akap9	UTSW	5	3,968,737 (GRCm38)	missense	probably benign	0.41
R4756:Akap9	UTSW	5	4,001,418 (GRCm38)	missense	probably damaging	0.99
R4757:Akap9	UTSW	5	4,008,382 (GRCm38)	missense	probably damaging	1.00
R4860:Akap9	UTSW	5	4,034,916 (GRCm38)	intron	probably benign
R4937:Akap9	UTSW	5	4,050,145 (GRCm38)	splice site	probably null
R4960:Akap9	UTSW	5	3,957,664 (GRCm38)	missense	probably benign	0.15
R4974:Akap9	UTSW	5	3,961,466 (GRCm38)	missense	possibly damaging	0.81
R5101:Akap9	UTSW	5	4,001,748 (GRCm38)	missense	probably damaging	0.96
R5160:Akap9	UTSW	5	4,030,007 (GRCm38)	missense	probably damaging	1.00
R5200:Akap9	UTSW	5	3,960,734 (GRCm38)	missense	probably benign	0.00
R5245:Akap9	UTSW	5	3,976,209 (GRCm38)	missense	probably damaging	0.99
R5293:Akap9	UTSW	5	3,948,687 (GRCm38)	missense	probably damaging	0.99
R5408:Akap9	UTSW	5	4,058,458 (GRCm38)	missense	possibly damaging	0.84
R5507:Akap9	UTSW	5	3,968,683 (GRCm38)	missense	probably benign	0.41
R5517:Akap9	UTSW	5	4,001,665 (GRCm38)	missense	possibly damaging	0.76
R5579:Akap9	UTSW	5	4,064,714 (GRCm38)	missense	possibly damaging	0.93
R5619:Akap9	UTSW	5	3,954,760 (GRCm38)	intron	probably benign
R5645:Akap9	UTSW	5	4,050,590 (GRCm38)	missense	probably benign	0.09
R5669:Akap9	UTSW	5	4,050,540 (GRCm38)	nonsense	probably null
R5686:Akap9	UTSW	5	3,971,926 (GRCm38)	missense	probably benign	0.00
R5697:Akap9	UTSW	5	3,960,170 (GRCm38)	missense	possibly damaging	0.92
R5821:Akap9	UTSW	5	4,046,064 (GRCm38)	missense	probably benign	0.13
R5875:Akap9	UTSW	5	4,077,285 (GRCm38)	missense	probably benign	0.01
R5897:Akap9	UTSW	5	4,077,904 (GRCm38)	missense	probably benign	0.23
R5999:Akap9	UTSW	5	4,043,925 (GRCm38)	missense	probably damaging	1.00
R6025:Akap9	UTSW	5	4,032,801 (GRCm38)	missense	probably damaging	1.00
R6078:Akap9	UTSW	5	4,067,924 (GRCm38)	critical splice donor site	probably null
R6138:Akap9	UTSW	5	4,067,924 (GRCm38)	critical splice donor site	probably null
R6225:Akap9	UTSW	5	3,962,105 (GRCm38)	missense	probably damaging	1.00
R6243:Akap9	UTSW	5	4,065,000 (GRCm38)	splice site	probably null
R6326:Akap9	UTSW	5	3,962,061 (GRCm38)	missense	probably damaging	1.00
R6564:Akap9	UTSW	5	4,028,491 (GRCm38)	missense	probably damaging	0.98
R6617:Akap9	UTSW	5	3,968,745 (GRCm38)	missense	probably benign	0.04
R6625:Akap9	UTSW	5	3,968,745 (GRCm38)	missense	probably benign	0.04
R6632:Akap9	UTSW	5	4,013,842 (GRCm38)	splice site	probably null
R6677:Akap9	UTSW	5	4,029,869 (GRCm38)	missense	probably benign	0.21
R6717:Akap9	UTSW	5	4,064,086 (GRCm38)	missense	probably damaging	1.00
R6893:Akap9	UTSW	5	3,961,709 (GRCm38)	missense	probably benign	0.32
R6915:Akap9	UTSW	5	3,960,551 (GRCm38)	missense	probably benign	0.03
R6938:Akap9	UTSW	5	4,046,628 (GRCm38)	missense	possibly damaging	0.91
R6972:Akap9	UTSW	5	4,046,699 (GRCm38)	missense	possibly damaging	0.62
R6973:Akap9	UTSW	5	4,046,699 (GRCm38)	missense	possibly damaging	0.62
R6993:Akap9	UTSW	5	4,065,866 (GRCm38)	missense	possibly damaging	0.65
R7032:Akap9	UTSW	5	3,954,896 (GRCm38)	missense	probably benign
R7164:Akap9	UTSW	5	4,060,364 (GRCm38)	missense	probably damaging	0.96
R7170:Akap9	UTSW	5	3,968,745 (GRCm38)	missense	probably benign	0.04
R7192:Akap9	UTSW	5	4,005,723 (GRCm38)	splice site	probably null
R7284:Akap9	UTSW	5	3,956,246 (GRCm38)	missense	probably damaging	1.00
R7299:Akap9	UTSW	5	4,032,696 (GRCm38)	missense	probably damaging	1.00
R7313:Akap9	UTSW	5	4,004,933 (GRCm38)	missense	probably damaging	1.00
R7326:Akap9	UTSW	5	4,045,930 (GRCm38)	missense	possibly damaging	0.47
R7343:Akap9	UTSW	5	4,046,364 (GRCm38)	missense	probably damaging	0.99
R7455:Akap9	UTSW	5	3,972,792 (GRCm38)	missense	probably benign	0.03
R7482:Akap9	UTSW	5	3,968,745 (GRCm38)	missense	probably benign	0.04
R7489:Akap9	UTSW	5	4,004,933 (GRCm38)	missense	probably damaging	1.00
R7525:Akap9	UTSW	5	3,968,745 (GRCm38)	missense	probably benign	0.04
R7528:Akap9	UTSW	5	3,968,745 (GRCm38)	missense	probably benign	0.04
R7576:Akap9	UTSW	5	3,968,745 (GRCm38)	missense	probably benign	0.04
R7577:Akap9	UTSW	5	3,968,745 (GRCm38)	missense	probably benign	0.04
R7578:Akap9	UTSW	5	3,968,745 (GRCm38)	missense	probably benign	0.04
R7610:Akap9	UTSW	5	3,957,677 (GRCm38)	missense	possibly damaging	0.95
R7658:Akap9	UTSW	5	3,968,745 (GRCm38)	missense	probably benign	0.04
R7754:Akap9	UTSW	5	4,046,736 (GRCm38)	missense	probably benign	0.03
R7818:Akap9	UTSW	5	4,013,875 (GRCm38)	nonsense	probably null
R7979:Akap9	UTSW	5	4,050,381 (GRCm38)	missense	probably benign
R7991:Akap9	UTSW	5	4,064,949 (GRCm38)	splice site	probably null
R8036:Akap9	UTSW	5	4,070,397 (GRCm38)	nonsense	probably null
R8054:Akap9	UTSW	5	4,038,707 (GRCm38)	critical splice donor site	probably null
R8116:Akap9	UTSW	5	4,061,183 (GRCm38)	missense	probably benign	0.04
R8150:Akap9	UTSW	5	3,961,982 (GRCm38)	missense	probably damaging	1.00
R8234:Akap9	UTSW	5	4,044,845 (GRCm38)	missense	probably benign	0.18
R8348:Akap9	UTSW	5	3,948,897 (GRCm38)	critical splice donor site	probably null
R8365:Akap9	UTSW	5	3,968,745 (GRCm38)	missense	probably benign	0.04
R8366:Akap9	UTSW	5	3,968,745 (GRCm38)	missense	probably benign	0.04
R8448:Akap9	UTSW	5	3,948,897 (GRCm38)	critical splice donor site	probably null
R8466:Akap9	UTSW	5	4,038,659 (GRCm38)	missense	probably damaging	1.00
R8772:Akap9	UTSW	5	4,046,255 (GRCm38)	missense	probably damaging	1.00
R8881:Akap9	UTSW	5	3,961,279 (GRCm38)	missense
R8937:Akap9	UTSW	5	4,044,048 (GRCm38)	missense	possibly damaging	0.78
R8956:Akap9	UTSW	5	3,948,805 (GRCm38)	missense	possibly damaging	0.79
R9000:Akap9	UTSW	5	4,055,650 (GRCm38)	missense	probably benign
R9049:Akap9	UTSW	5	4,064,597 (GRCm38)	missense
R9074:Akap9	UTSW	5	4,077,959 (GRCm38)	missense	probably benign	0.40
R9124:Akap9	UTSW	5	4,061,284 (GRCm38)	missense	probably damaging	0.99
R9129:Akap9	UTSW	5	4,069,089 (GRCm38)	missense	probably benign	0.00
R9371:Akap9	UTSW	5	3,961,852 (GRCm38)	missense	possibly damaging	0.83
R9424:Akap9	UTSW	5	3,962,224 (GRCm38)	nonsense	probably null
R9424:Akap9	UTSW	5	3,962,223 (GRCm38)	nonsense	probably null
R9509:Akap9	UTSW	5	4,046,349 (GRCm38)	missense	probably benign
R9515:Akap9	UTSW	5	4,055,709 (GRCm38)	missense	probably damaging	1.00
R9567:Akap9	UTSW	5	4,077,311 (GRCm38)	missense	possibly damaging	0.89
R9587:Akap9	UTSW	5	4,069,149 (GRCm38)	missense	probably damaging	1.00
R9619:Akap9	UTSW	5	4,044,833 (GRCm38)	missense	probably damaging	1.00
R9635:Akap9	UTSW	5	4,050,545 (GRCm38)	missense	probably benign	0.20
R9680:Akap9	UTSW	5	3,961,587 (GRCm38)	missense	probably benign	0.03
R9691:Akap9	UTSW	5	3,960,491 (GRCm38)	missense	probably damaging	1.00
R9726:Akap9	UTSW	5	4,003,757 (GRCm38)	missense	probably benign	0.39
U15987:Akap9	UTSW	5	4,067,924 (GRCm38)	critical splice donor site	probably null
X0026:Akap9	UTSW	5	4,014,039 (GRCm38)	missense	probably damaging	1.00
X0057:Akap9	UTSW	5	3,975,598 (GRCm38)	critical splice acceptor site	probably null
Z1176:Akap9	UTSW	5	3,962,251 (GRCm38)	missense	probably damaging	0.96
Z1177:Akap9	UTSW	5	4,046,189 (GRCm38)	missense	probably damaging	1.00

Predicted Primers

PCR Primer
(F):5'- TGGTTGAGAAACTGCAGCAG -3'
(R):5'- AGGGCTTTTCTGAAGACGCG -3'

Sequencing Primer
(F):5'- CAGGAGGTGGTCAGCACTG -3'
(R):5'- CTTTTCTGAAGACGCGTAAGG -3'

Posted On

2019-06-07