Mutagenetix > Incidental Mutations

Incidental Mutation 'PIT4445001:Zscan25'

555400

Institutional Source

Beutler Lab

Gene Symbol

Zscan25

Ensembl Gene

ENSMUSG00000070420

Gene Name

zinc finger and SCAN domain containing 25

Synonyms

Zfp498, EG666311

Accession Numbers

Is this an essential gene?

Probably non essential (E-score: 0.158) question?

Stock #

PIT4445001 (G1)

Quality Score

225.009

Status

Not validated

Chromosome

Chromosomal Location

145280500-145291278 bp(+) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

T to A at 145290612 bp

Zygosity

Heterozygous

Amino Acid Change

Valine to Aspartic acid at position 362 (V362D)

Ref Sequence

ENSEMBL: ENSMUSP00000091664 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000094116] [ENSMUST00000199563] [ENSMUST00000200246]

Predicted Effect

probably damaging
Transcript: ENSMUST00000094116
AA Change: V362D

PolyPhen 2 Score 0.999 (Sensitivity: 0.14; Specificity: 0.99)

SMART Domains

Protein: ENSMUSP00000091664
Gene: ENSMUSG00000070420
AA Change: V362D

Domain	Start	End	E-Value	Type
SCAN	38	150	5.94e-53	SMART
Blast:KRAB	231	288	4e-31	BLAST
ZnF_C2H2	346	368	2.15e-5	SMART
ZnF_C2H2	374	396	5.06e-2	SMART
ZnF_C2H2	402	424	8.34e-3	SMART
ZnF_C2H2	430	452	3.39e-3	SMART
ZnF_C2H2	458	479	1.08e1	SMART
ZnF_C2H2	485	507	4.47e-3	SMART
ZnF_C2H2	513	536	5.59e-4	SMART

Predicted Effect

probably benign
Transcript: ENSMUST00000199563

SMART Domains

Protein: ENSMUSP00000143449
Gene: ENSMUSG00000070420

Domain	Start	End	E-Value	Type
SCAN	38	150	2.1e-54	SMART

Predicted Effect

probably benign
Transcript: ENSMUST00000200246

SMART Domains

Protein: ENSMUSP00000142367
Gene: ENSMUSG00000070420

Domain	Start	End	E-Value	Type
SCAN	38	150	1.9e-55	SMART

Coding Region Coverage

1x: 93.5%
3x: 90.8%
10x: 84.5%
20x: 70.9%

Validation Efficiency

MGI Phenotype

FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] This gene encodes a protein that bears some similarity to zinc finger proteins, which are involved in DNA binding and protein-protein interactions. Multiple alternatively spliced transcript variants have been identified, but the full-length nature for most of them has not been determined. [provided by RefSeq, Jul 2008]

Allele List at MGI

Other mutations in this stock

Total: 73 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Abca8a	T	C	11: 110,075,551	K419E	probably damaging	Het
Adam7	T	A	14: 68,509,748	K587N	possibly damaging	Het
Agl	T	C	3: 116,771,460	M382V		Het
Agpat3	A	T	10: 78,274,093	F341I	possibly damaging	Het
Ampd2	A	T	3: 108,075,012	L767Q	probably damaging	Het
Arhgap28	A	T	17: 67,896,235	D124E	possibly damaging	Het
Arhgap32	T	C	9: 32,260,856	L1644P	probably damaging	Het
Asic4	T	C	1: 75,451,127	V99A	probably benign	Het
Asns	T	A	6: 7,689,277	N75I	probably damaging	Het
Atp10a	A	G	7: 58,803,467	D798G	probably damaging	Het
Atp8a1	A	G	5: 67,622,660	V1145A		Het
BC017158	A	G	7: 128,276,534	V243A	probably benign	Het
Ccdc171	A	G	4: 83,661,747	Q577R	probably damaging	Het
Cd247	T	A	1: 165,861,036	D154E	probably damaging	Het
Ceacam1	A	T	7: 25,476,456	N104K	probably damaging	Het
Chfr	A	G	5: 110,151,677	D312G	possibly damaging	Het
Ddb1	C	A	19: 10,625,970	L881I	probably damaging	Het
Dgkh	A	T	14: 78,575,942	I1032N	possibly damaging	Het
Efcab6	A	T	15: 83,904,267	V942D	probably benign	Het
Fmo5	A	G	3: 97,651,528	T435A	probably benign	Het
Fpr1	T	C	17: 17,876,893	Q278R	probably benign	Het
Fzr1	C	T	10: 81,369,394	W256*	probably null	Het
Gabrb1	T	C	5: 72,108,782	S261P	probably damaging	Het
Galr2	G	T	11: 116,281,648	A55S	probably benign	Het
Gbp2	A	T	3: 142,637,466	K581N	probably benign	Het
Gm7682	T	G	5: 94,448,507	W468G	probably benign	Het
Gria1	A	T	11: 57,185,838	Y89F	probably damaging	Het
Ibsp	T	A	5: 104,302,304	I26N	possibly damaging	Het
Igf1r	T	C	7: 68,207,463	F1058L	probably damaging	Het
Ighv1-16	A	C	12: 114,666,060	C36G	probably benign	Het
Igll1	T	C	16: 16,860,919	T176A	probably benign	Het
Ilkap	T	C	1: 91,385,345	T143A	probably benign	Het
Kdm3b	A	T	18: 34,793,115	K103*	probably null	Het
Mphosph9	A	G	5: 124,298,790	I497T	possibly damaging	Het
Mrgpra3	G	A	7: 47,590,160	T6I	possibly damaging	Het
Mrpl38	T	C	11: 116,132,558		probably null	Het
Myo3a	A	T	2: 22,542,415	E813V	possibly damaging	Het
Myo7b	G	T	18: 31,959,466	Q2093K	possibly damaging	Het
Myo7b	T	C	18: 31,962,352	K1851R	probably damaging	Het
P3h2	T	A	16: 25,984,999	D339V	probably benign	Het
Pcdhb6	C	A	18: 37,335,247	P407Q	possibly damaging	Het
Plch2	C	A	4: 155,009,026	R153L	probably damaging	Het
Plppr4	G	A	3: 117,360,308		probably benign	Het
Ppp1r3a	A	G	6: 14,717,777	F1046S	probably damaging	Het
Prkacb	A	T	3: 146,755,691	L107M	probably benign	Het
Ranbp17	A	G	11: 33,481,020		probably null	Het
Rasl11b	C	A	5: 74,197,333	P88Q	probably damaging	Het
Rcc2	A	G	4: 140,721,149	E503G	possibly damaging	Het
Rnf216	T	A	5: 143,086,003	K407N	probably damaging	Het
Scrn3	G	A	2: 73,318,329	M81I	possibly damaging	Het
Scube2	T	C	7: 109,809,180	T687A	probably benign	Het
Serpinb10	T	A	1: 107,535,998	F3L	probably benign	Het
Sgcb	C	T	5: 73,639,812	V202I	probably damaging	Het
Sgce	A	G	6: 4,689,654	V429A	possibly damaging	Het
Sh3rf2	T	C	18: 42,153,164	V574A	probably benign	Het
Sncaip	T	C	18: 52,868,944	L179S	probably damaging	Het
Sntg2	A	T	12: 30,312,572	D58E	probably damaging	Het
Taar3	T	C	10: 23,949,688	I44T	possibly damaging	Het
Tmem87b	G	A	2: 128,831,471	V227I	probably benign	Het
Tnfsf9	T	C	17: 57,105,517	L29P	possibly damaging	Het
Tnip2	T	C	5: 34,496,871	H391R	probably benign	Het
Traf5	A	G	1: 191,997,807	Y125H		Het
Ttc23	T	G	7: 67,667,213	I77M	probably damaging	Het
Ube2l3	T	C	16: 17,160,172	N43S	probably benign	Het
Vars2	A	T	17: 35,666,211	C142*	probably null	Het
Vmn2r15	A	C	5: 109,287,142	D565E	probably damaging	Het
Vmn2r56	C	T	7: 12,715,226		probably null	Het
Vmn2r79	A	T	7: 87,002,200	D269V	possibly damaging	Het
Vmn2r85	A	T	10: 130,425,703	M255K	probably benign	Het
Wfdc6a	T	A	2: 164,579,826	*137C	probably null	Het
Wipi2	T	C	5: 142,666,884	V417A	probably benign	Het
Xirp2	G	T	2: 67,509,772	V786L	possibly damaging	Het
Zdhhc13	G	A	7: 48,795,949	G26S	probably benign	Het

Other mutations in Zscan25

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00309:Zscan25	APN	5	145283749	missense	probably damaging	1.00
IGL02622:Zscan25	APN	5	145290702	missense	probably damaging	1.00
IGL02881:Zscan25	APN	5	145290486	missense	probably benign	0.25
R1713:Zscan25	UTSW	5	145283691	missense	probably damaging	0.99
R1828:Zscan25	UTSW	5	145291048	missense	possibly damaging	0.95
R2233:Zscan25	UTSW	5	145283692	missense	probably damaging	1.00
R3882:Zscan25	UTSW	5	145291052	missense	probably damaging	1.00
R4330:Zscan25	UTSW	5	145290551	missense	probably damaging	0.99
R4527:Zscan25	UTSW	5	145283458	missense	probably damaging	1.00
R4539:Zscan25	UTSW	5	145288391	missense	probably benign	0.01
R4598:Zscan25	UTSW	5	145291005	missense	probably benign	0.30
R4611:Zscan25	UTSW	5	145291116	missense	probably damaging	1.00
R4662:Zscan25	UTSW	5	145286310	missense	unknown
R6733:Zscan25	UTSW	5	145290913	unclassified	probably null
R6751:Zscan25	UTSW	5	145290563	missense	probably damaging	1.00
R7161:Zscan25	UTSW	5	145286441	missense	probably benign	0.00
R7912:Zscan25	UTSW	5	145290511	missense	probably benign	0.00
R7993:Zscan25	UTSW	5	145290511	missense	probably benign	0.00

Predicted Primers

PCR Primer
(F):5'- AAGCCGACATCCAGATTCCTG -3'
(R):5'- CCAGCAGTCTGAGCACTTGTAG -3'

Sequencing Primer
(F):5'- AGCTCTGGGACCAGTCT -3'
(R):5'- TCTCCCCAGTGTGGCTACG -3'

Posted On

2019-06-07