Mutagenetix > Incidental Mutation

Incidental Mutation 'PIT4508001:Ogfrl1'

556213

Institutional Source

Beutler Lab

Gene Symbol

Ogfrl1

Ensembl Gene

ENSMUSG00000026158

Gene Name

opioid growth factor receptor-like 1

Synonyms

2210417C17Rik

Accession Numbers

Is this an essential gene?

Probably non essential (E-score: 0.129) question?

Stock #

PIT4508001 (G1)

Quality Score

225.009

Status

Not validated

Chromosome

Chromosomal Location

23366424-23397772 bp(-) (GRCm38)

Type of Mutation

nonsense

DNA Base Change (assembly)

T to A at 23370270 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Arginine to Stop codon at position 292 (R292*)

Ref Sequence

ENSEMBL: ENSMUSP00000027343 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000027343] [ENSMUST00000188677]

AlphaFold

Q8VE52

Predicted Effect

probably null
Transcript: ENSMUST00000027343
AA Change: R292*

SMART Domains

Protein: ENSMUSP00000027343
Gene: ENSMUSG00000026158
AA Change: R292*

Domain	Start	End	E-Value	Type
low complexity region	28	44	N/A	INTRINSIC
low complexity region	72	86	N/A	INTRINSIC
Pfam:OGFr_N	114	320	1.7e-104	PFAM

Predicted Effect

probably null
Transcript: ENSMUST00000188677
AA Change: R92*

SMART Domains

Protein: ENSMUSP00000139453
Gene: ENSMUSG00000026158
AA Change: R92*

Domain	Start	End	E-Value	Type
Pfam:OGFr_N	1	92	1.6e-38	PFAM

Coding Region Coverage

1x: 93.4%
3x: 90.7%
10x: 84.6%
20x: 71.6%

Validation Efficiency

Allele List at MGI

Other mutations in this stock

Total: 34 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Abcc5	T	C	16: 20,357,378	T1228A	probably damaging	Het
Actg2	A	T	6: 83,513,007	I370N	possibly damaging	Het
Ankrd36	A	G	11: 5,607,137	T330A	possibly damaging	Het
Atrip	C	T	9: 109,073,989	A6T	possibly damaging	Het
Bms1	G	A	6: 118,383,806	R1234C	probably benign	Het
Cacnb2	A	T	2: 14,984,419	T487S	probably benign	Het
Col4a3bp	T	C	13: 96,630,776	F478S	probably damaging	Het
Esco2	A	T	14: 65,831,465	V132E	probably damaging	Het
Gjb5	A	T	4: 127,356,240	L37Q	probably damaging	Het
Gm8020	T	A	14: 42,502,277	Y24F		Het
H2-Q6	T	C	17: 35,425,820	L195P	probably damaging	Het
Hc	T	C	2: 34,984,804	T1602A	probably damaging	Het
Hcrtr2	A	T	9: 76,246,380	Y243*	probably null	Het
Hist1h2bg	CTCGACCATCACGTC	CTC	13: 23,571,693		probably benign	Het
Itga3	T	C	11: 95,055,893	H730R	probably benign	Het
Kif1a	A	T	1: 93,046,729	L866Q	probably damaging	Het
Krt81	A	T	15: 101,462,725	L127Q	probably damaging	Het
Mdn1	T	C	4: 32,719,223	I2262T	probably damaging	Het
Mxd3	A	T	13: 55,325,894	D170E	probably benign	Het
Myh2	A	G	11: 67,185,505	M811V	probably benign	Het
Naa16	A	T	14: 79,369,087	D335E	probably benign	Het
Nkd1	A	G	8: 88,522,400	T58A	probably benign	Het
Olfr1136	G	A	2: 87,693,715	H56Y	probably damaging	Het
Olfr589	T	A	7: 103,155,313	M145L	probably benign	Het
Pom121l12	A	G	11: 14,599,689	R132G	possibly damaging	Het
Prss46	A	G	9: 110,851,416	K210E	probably damaging	Het
Scn8a	T	C	15: 101,029,692	Y1351H	probably damaging	Het
Shisa9	G	A	16: 12,267,480	V318I	probably benign	Het
Slc41a2	T	G	10: 83,254,880	H480P	probably damaging	Het
Slc45a1	C	A	4: 150,638,435	A331S	probably benign	Het
Smg1	A	T	7: 118,185,541	F885I	unknown	Het
Speer4f1	T	C	5: 17,480,414	S236P	unknown	Het
Zfp326	A	T	5: 105,914,690	Q475L	probably benign	Het
Zzef1	T	G	11: 72,895,176	V2058G	probably benign	Het

Other mutations in Ogfrl1

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00913:Ogfrl1	APN	1	23370090	missense	probably benign
IGL02200:Ogfrl1	APN	1	23370069	missense	probably benign	0.00
R0012:Ogfrl1	UTSW	1	23370125	missense	possibly damaging	0.83
R0735:Ogfrl1	UTSW	1	23375754	missense	possibly damaging	0.76
R1474:Ogfrl1	UTSW	1	23375809	missense	probably damaging	1.00
R3837:Ogfrl1	UTSW	1	23369960	missense	probably benign	0.03
R4037:Ogfrl1	UTSW	1	23378964	splice site	probably benign
R4039:Ogfrl1	UTSW	1	23378964	splice site	probably benign
R4332:Ogfrl1	UTSW	1	23375829	missense	probably damaging	1.00
R4780:Ogfrl1	UTSW	1	23370321	missense	probably damaging	1.00
R5056:Ogfrl1	UTSW	1	23379049	missense	probably damaging	0.97
R5994:Ogfrl1	UTSW	1	23378989	missense	probably damaging	1.00
R6167:Ogfrl1	UTSW	1	23376228	missense	probably damaging	1.00
R6340:Ogfrl1	UTSW	1	23369863	missense	probably benign	0.04
R6341:Ogfrl1	UTSW	1	23369863	missense	probably benign	0.04
R6342:Ogfrl1	UTSW	1	23369863	missense	probably benign	0.04
R6343:Ogfrl1	UTSW	1	23369863	missense	probably benign	0.04
R6363:Ogfrl1	UTSW	1	23370113	missense	probably benign	0.01
R6584:Ogfrl1	UTSW	1	23369863	missense	probably benign	0.04
R6586:Ogfrl1	UTSW	1	23369863	missense	probably benign	0.04
R7419:Ogfrl1	UTSW	1	23382982	nonsense	probably null
R8364:Ogfrl1	UTSW	1	23375743	nonsense	probably null
R8749:Ogfrl1	UTSW	1	23370318	missense	probably damaging	1.00
R9539:Ogfrl1	UTSW	1	23376241	missense	probably damaging	1.00

Predicted Primers

PCR Primer
(F):5'- ATTTACGTGACTAGCTCCTGGGG -3'
(R):5'- AATGCTCTCTTTCAGGTCTCAG -3'

Sequencing Primer
(F):5'- ACTAGCTCCTGGGGAAATTTTG -3'
(R):5'- CAGGTCTCAGCACAACTATTTAAG -3'

Posted On

2019-06-07