Mutagenetix > Incidental Mutations

Incidental Mutation 'PIT4585001:Tas2r104'

556599

Institutional Source

Beutler Lab

Gene Symbol

Tas2r104

Ensembl Gene

ENSMUSG00000061977

Gene Name

taste receptor, type 2, member 104

Synonyms

T2R04, mGR04, Tas2r4, mt2r45

Accession Numbers

Is this an essential gene?

Probably non essential (E-score: 0.054) question?

Stock #

PIT4585001 (G1)

Quality Score

225.009

Status

Not validated

Chromosome

Chromosomal Location

131684836-131685744 bp(-) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

T to C at 131685558 bp

Zygosity

Heterozygous

Amino Acid Change

Threonine to Alanine at position 63 (T63A)

Ref Sequence

ENSEMBL: ENSMUSP00000072237 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000053652] [ENSMUST00000072404] [ENSMUST00000080619]

Predicted Effect

probably benign
Transcript: ENSMUST00000053652

SMART Domains

Protein: ENSMUSP00000058006
Gene: ENSMUSG00000051153

Domain	Start	End	E-Value	Type
Pfam:TAS2R	1	298	9.4e-109	PFAM

Predicted Effect

possibly damaging
Transcript: ENSMUST00000072404
AA Change: T63A

PolyPhen 2 Score 0.495 (Sensitivity: 0.88; Specificity: 0.90)

SMART Domains

Protein: ENSMUSP00000072237
Gene: ENSMUSG00000061977
AA Change: T63A

Domain	Start	End	E-Value	Type
Pfam:TAS2R	1	298	8.3e-102	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000080619

SMART Domains

Protein: ENSMUSP00000079453
Gene: ENSMUSG00000063478

Domain	Start	End	E-Value	Type
Pfam:TAS2R	1	298	8.1e-104	PFAM

Coding Region Coverage

1x: 93.6%
3x: 91.2%
10x: 86.5%
20x: 76.8%

Validation Efficiency

Allele List at MGI

Other mutations in this stock

Total: 55 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Aurka	T	C	2: 172,357,197	M318V	probably benign	Het
Cacna2d1	T	A	5: 16,326,344	D560E	probably damaging	Het
Ccdc148	T	A	2: 58,982,976	T202S	probably benign	Het
Ccdc155	C	T	7: 45,200,271	G76D	probably benign	Het
Cdc42bpb	T	C	12: 111,304,978	D1149G	probably damaging	Het
Clasp1	T	A	1: 118,462,555	N156K	probably damaging	Het
Cox18	G	A	5: 90,217,575	T255I	possibly damaging	Het
Cse1l	A	G	2: 166,941,474	T783A	probably damaging	Het
Dnajc16	T	C	4: 141,764,685	Y609C	probably damaging	Het
Doc2g	A	G	19: 4,006,630	T339A	probably benign	Het
Eif5a	T	C	11: 69,918,070		probably benign	Het
Epha3	A	G	16: 63,566,577		probably null	Het
Esco1	A	T	18: 10,594,355	C310*	probably null	Het
Fam208b	G	A	13: 3,574,979	A1657V	possibly damaging	Het
Fam222a	A	G	5: 114,611,040	Y99C	probably damaging	Het
Fzd2	T	C	11: 102,605,747	L339P	probably damaging	Het
Gfral	A	T	9: 76,197,294	N145K	probably damaging	Het
Gga1	T	A	15: 78,893,790	N618K	probably benign	Het
Gpatch3	T	A	4: 133,583,086	H447Q	probably damaging	Het
Gpn1	A	T	5: 31,509,403	R346*	probably null	Het
Gsg1	T	C	6: 135,237,560	E317G	probably benign	Het
Gsk3b	A	G	16: 38,184,454	N129S	probably damaging	Het
Hmg20b	G	T	10: 81,348,955	D94E	possibly damaging	Het
Klhdc9	T	A	1: 171,359,818	H204L	possibly damaging	Het
Klhl24	A	G	16: 20,106,888	I55M	probably benign	Het
Kmt2c	T	C	5: 25,315,106	D2002G	probably benign	Het
Lama4	A	G	10: 39,074,746	N1015S	probably damaging	Het
Lpp	T	C	16: 24,761,947	C263R	probably benign	Het
Lrp1b	T	C	2: 41,269,204	I1689V		Het
Mipep	C	A	14: 60,784,835	Q50K	probably benign	Het
Mx1	T	C	16: 97,456,254	D101G	probably benign	Het
Nabp2	C	G	10: 128,408,807	E37Q	possibly damaging	Het
Nme6	A	G	9: 109,842,036	I115V	possibly damaging	Het
Nup93	A	T	8: 94,243,727	T85S	probably benign	Het
Oit3	T	A	10: 59,431,013	I224F	possibly damaging	Het
Parp14	T	C	16: 35,858,605	K331R	probably benign	Het
Pls1	T	A	9: 95,761,390	T519S	probably benign	Het
Rcn3	A	G	7: 45,086,694	F197L	probably benign	Het
Rnf213	C	T	11: 119,458,392	T3773I		Het
Rprd1b	A	T	2: 158,047,957	I153L	probably benign	Het
Scel	A	G	14: 103,592,368	D462G	possibly damaging	Het
Sh3bp1	C	T	15: 78,910,076	S548L	possibly damaging	Het
Sim1	T	A	10: 50,984,188	Y715*	probably null	Het
Slc18a2	A	T	19: 59,293,861	Q500L	possibly damaging	Het
Slc5a8	T	G	10: 88,886,503	M66R	probably damaging	Het
Slco1a6	T	C	6: 142,109,520	T233A	probably damaging	Het
Smu1	T	C	4: 40,739,623	T396A	probably benign	Het
Top2a	C	T	11: 99,001,373	A1088T	probably benign	Het
Ucp1	T	C	8: 83,293,948	F129S	probably damaging	Het
Unc13b	T	A	4: 43,091,298	D41E	probably benign	Het
Usp10	T	G	8: 119,954,892	V696G	probably benign	Het
Xylt2	C	T	11: 94,666,240	V745M	probably damaging	Het
Zbtb49	A	T	5: 38,216,476	N41K	probably damaging	Het
Zfp109	T	A	7: 24,229,354	D218V	probably benign	Het
Zfp420	G	A	7: 29,876,005	R550Q	probably benign	Het

Other mutations in Tas2r104

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00590:Tas2r104	APN	6	131685567	missense	probably damaging	1.00
IGL01390:Tas2r104	APN	6	131685485	missense	probably benign	0.13
IGL02751:Tas2r104	APN	6	131685144	missense	probably damaging	1.00
R0218:Tas2r104	UTSW	6	131685092	missense	probably damaging	1.00
R0453:Tas2r104	UTSW	6	131685341	missense	probably benign	0.00
R0472:Tas2r104	UTSW	6	131685471	missense	probably benign	0.06
R0614:Tas2r104	UTSW	6	131685202	missense	probably damaging	1.00
R1290:Tas2r104	UTSW	6	131684845	nonsense	probably null
R1480:Tas2r104	UTSW	6	131685294	missense	probably benign	0.17
R1698:Tas2r104	UTSW	6	131685584	missense	probably damaging	1.00
R2050:Tas2r104	UTSW	6	131685120	missense	probably damaging	1.00
R2229:Tas2r104	UTSW	6	131685132	missense	probably damaging	1.00
R3824:Tas2r104	UTSW	6	131685039	missense	possibly damaging	0.55
R3852:Tas2r104	UTSW	6	131684925	missense	probably benign	0.09
R4283:Tas2r104	UTSW	6	131685411	missense	probably damaging	1.00
R4583:Tas2r104	UTSW	6	131685435	missense	probably benign	0.00
R4710:Tas2r104	UTSW	6	131685444	missense	probably damaging	0.96
R4954:Tas2r104	UTSW	6	131685005	missense	probably damaging	0.99
R5559:Tas2r104	UTSW	6	131685131	missense	probably damaging	1.00
R5765:Tas2r104	UTSW	6	131685273	missense	probably benign
R5843:Tas2r104	UTSW	6	131684975	missense	probably damaging	0.99
R7304:Tas2r104	UTSW	6	131685042	missense	possibly damaging	0.90

Predicted Primers

PCR Primer
(F):5'- CTTCATCCAGAGAAATATGTAGTGGG -3'
(R):5'- TCCAGCAGAAGCCTTCTTGG -3'

Sequencing Primer
(F):5'- AGTGGGAAAAATTTGCTATCTTCAG -3'
(R):5'- AAGCCTTCTTGGAAACTGGC -3'

Posted On

2019-06-07