Mutagenetix > Incidental Mutation

Incidental Mutation 'R0605:Cry1'

55667

Institutional Source

Beutler Lab

Gene Symbol

Cry1

Ensembl Gene

ENSMUSG00000020038

Gene Name

cryptochrome 1 (photolyase-like)

Synonyms

Phll1

MMRRC Submission

038794-MU

Accession Numbers

Essential gene?

Non essential (E-score: 0.000) question?

Stock #

R0605 (G1)

Quality Score

173

Status

Validated

Chromosome

Chromosomal Location

85131700-85185064 bp(-) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

T to C at 85184359 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Aspartic acid to Glycine at position 38 (D38G)

Ref Sequence

ENSEMBL: ENSMUSP00000020227 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000020227]

AlphaFold

P97784

PDB Structure

Crystal Structure of Mouse Cryptochrome1 in Complex with Period2 [X-RAY DIFFRACTION]
Crystal structure of mouse Cryptochrome 1 [X-RAY DIFFRACTION]

Predicted Effect

probably damaging
Transcript: ENSMUST00000020227
AA Change: D38G

PolyPhen 2 Score 0.964 (Sensitivity: 0.78; Specificity: 0.95)

SMART Domains

Protein: ENSMUSP00000020227
Gene: ENSMUSG00000020038
AA Change: D38G

Domain	Start	End	E-Value	Type
Pfam:DNA_photolyase	5	168	1.4e-47	PFAM
Pfam:FAD_binding_7	213	486	6.9e-91	PFAM
internal_repeat_1	502	523	3.57e-8	PROSPERO
internal_repeat_1	521	543	3.57e-8	PROSPERO
low complexity region	544	555	N/A	INTRINSIC

Meta Mutation Damage Score

0.8956

Coding Region Coverage

1x: 99.4%
3x: 98.9%
10x: 97.6%
20x: 95.4%

Validation Efficiency

100% (85/85)

MGI Phenotype

FUNCTION: This gene encodes a flavin adenine dinucleotide-binding protein that is a key component of the circadian core oscillator complex, which regulates the circadian clock. This gene is upregulated by Clock/Arntl heterodimers but then represses this upregulation in a feedback loop using Per/Cry heterodimers to interact with Clock/Arntl. Polymorphisms in this gene have been associated with altered sleep patterns. The encoded protein is widely conserved across plants and animals. Loss of this gene results in a shortened circadian cycle in complete darkness. [provided by RefSeq, Feb 2014]
PHENOTYPE: Homozygotes for targeted null mutations exhibit a one-hour shorter circadian period under constant darkness and reduced expression of another circadian gene in the suprachiasmatic nucleus in response to acute light exposure. [provided by MGI curators]

Allele List at MGI

Other mutations in this stock

Total: 84 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
4933406P04Rik	G	A	10: 20,311,227		probably benign	Het
Adam28	A	T	14: 68,606,600		probably benign	Het
Adamts3	A	G	5: 89,861,475	W110R	possibly damaging	Het
Add1	T	C	5: 34,614,224	V342A	possibly damaging	Het
Aff3	A	G	1: 38,209,987	S680P	probably damaging	Het
Ak9	T	C	10: 41,345,139	Y322H	probably damaging	Het
Als2	A	G	1: 59,168,414	L1528S	probably benign	Het
Ass1	A	T	2: 31,514,819	N371Y	probably damaging	Het
Atp6v1a	A	C	16: 44,111,496		probably null	Het
Bpi	T	C	2: 158,261,394	L103P	probably damaging	Het
Cd80	G	A	16: 38,482,694	V168I	probably benign	Het
Cfh	T	C	1: 140,102,358	S926G	probably damaging	Het
Chrd	A	T	16: 20,735,439	T304S	probably damaging	Het
Chsy3	A	G	18: 59,409,053	Y421C	probably damaging	Het
Cmbl	T	G	15: 31,585,309	V101G	probably damaging	Het
Colgalt2	T	A	1: 152,495,792		probably benign	Het
Coq4	C	T	2: 29,789,998	Q101*	probably null	Het
Cr2	T	C	1: 195,163,596		probably benign	Het
Dmxl2	T	C	9: 54,419,945	D758G	probably benign	Het
Epsti1	C	T	14: 77,927,237		probably benign	Het
Fam24b	T	C	7: 131,327,186		probably benign	Het
Fem1c	G	A	18: 46,505,160	R592C	probably benign	Het
Foxred1	T	C	9: 35,204,882	Y490C	possibly damaging	Het
Gm14124	A	G	2: 150,268,603	I404M	unknown	Het
Gm9875	A	G	2: 13,557,888	K9R	unknown	Het
Grid2ip	T	C	5: 143,379,362	S322P	probably damaging	Het
Gucy1b2	A	G	14: 62,403,159		probably benign	Het
Hmcn1	A	T	1: 150,657,376		probably null	Het
Hpdl	C	T	4: 116,820,787	S159N	possibly damaging	Het
Hsd17b12	A	T	2: 94,033,642	M285K	probably benign	Het
Icam5	T	C	9: 21,032,197	I23T	probably benign	Het
Kat5	A	G	19: 5,608,336		probably benign	Het
Lama3	A	G	18: 12,506,949	N67S	probably benign	Het
Lamb2	T	C	9: 108,486,105		probably benign	Het
Lgals3bp	A	G	11: 118,393,394	F453S	probably damaging	Het
Lypd4	A	G	7: 24,865,375	Y113H	probably damaging	Het
Mdm1	C	T	10: 118,146,601	T47M	probably damaging	Het
Mei1	C	A	15: 82,070,150	T52K	probably benign	Het
Meiob	G	A	17: 24,818,262		probably benign	Het
Ndufaf6	A	G	4: 11,051,224	V292A	probably damaging	Het
Neb	T	A	2: 52,264,026	M2358L	possibly damaging	Het
Nlrp1b	A	G	11: 71,156,179	S1119P	possibly damaging	Het
Nsmaf	A	G	4: 6,418,470		probably null	Het
Ogfod1	T	C	8: 94,047,267		probably benign	Het
Olfr1097	T	C	2: 86,890,419	Y252C	possibly damaging	Het
Olfr1410	G	T	1: 92,607,896	V20L	probably benign	Het
Olfr291	T	C	7: 84,857,137	I256T	probably damaging	Het
Osbpl1a	T	A	18: 12,882,279		probably null	Het
Otud7b	T	A	3: 96,144,959		probably benign	Het
P3h3	T	A	6: 124,856,035	H185L	probably damaging	Het
P4htm	G	A	9: 108,583,724	A183V	probably null	Het
Peak1	C	T	9: 56,227,098		probably benign	Het
Phf20l1	A	G	15: 66,595,122	K88R	probably damaging	Het
Phlpp2	A	G	8: 109,933,211	N721S	probably benign	Het
Plagl2	T	C	2: 153,235,944	K39R	probably benign	Het
Plppr1	A	T	4: 49,323,466	N252I	probably damaging	Het
Pom121l2	C	T	13: 21,982,036	A159V	probably damaging	Het
Prom2	C	A	2: 127,539,995		probably null	Het
Prrc2c	T	C	1: 162,682,426	T1017A	probably damaging	Het
Rimbp3	G	T	16: 17,211,699	A996S	probably damaging	Het
Rnf213	A	G	11: 119,431,717	T1387A	probably benign	Het
Scaper	A	T	9: 55,815,518		probably benign	Het
Scara5	A	G	14: 65,759,648	E403G	possibly damaging	Het
Scrib	T	C	15: 76,067,553	I94V	possibly damaging	Het
Shank3	T	C	15: 89,524,147	F67L	possibly damaging	Het
Shprh	T	C	10: 11,207,112	F1562L	probably damaging	Het
Src	C	T	2: 157,469,921	T529M	probably damaging	Het
Sycp2l	T	A	13: 41,143,466	M341K	probably benign	Het
Syde1	T	C	10: 78,589,095		probably benign	Het
Tarsl2	A	T	7: 65,678,071	R509S	probably damaging	Het
Tle6	T	A	10: 81,594,346	H324L	probably damaging	Het
Tnfaip8	ACTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTC	ACTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTC	18: 50,046,845		probably benign	Het
Tnfrsf14	T	A	4: 154,925,380	K115*	probably null	Het
Trappc10	T	C	10: 78,201,497	N824S	possibly damaging	Het
Tsc1	C	T	2: 28,671,778	S309F	probably damaging	Het
Ttc21a	A	G	9: 119,961,842	I885V	possibly damaging	Het
Ttn	C	T	2: 76,740,453	A26699T	probably damaging	Het
Ttn	T	C	2: 76,948,371	Y1262C	unknown	Het
Usp49	T	C	17: 47,674,926		probably null	Het
Vmn1r226	A	T	17: 20,687,871	T122S	probably benign	Het
Vps8	A	T	16: 21,559,337	T1033S	probably benign	Het
Vwf	C	A	6: 125,685,837	T2728K	probably benign	Het
Wdr5b	T	C	16: 36,041,996	S162P	probably benign	Het
Xrn1	C	T	9: 96,026,877	Q1235*	probably null	Het

Other mutations in Cry1

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00234:Cry1	APN	10	85146834	missense	probably benign	0.11
IGL00737:Cry1	APN	10	85143040	missense	probably benign	0.02
IGL01349:Cry1	APN	10	85148739	missense	probably benign	0.00
IGL01544:Cry1	APN	10	85146496	nonsense	probably null
IGL01545:Cry1	APN	10	85184362	missense	possibly damaging	0.94
IGL01767:Cry1	APN	10	85146474	missense	probably damaging	1.00
IGL03392:Cry1	APN	10	85157129	missense	possibly damaging	0.88
R0119:Cry1	UTSW	10	85133240	critical splice donor site	probably null
R1618:Cry1	UTSW	10	85146454	missense	probably damaging	1.00
R1955:Cry1	UTSW	10	85144178	missense	probably benign	0.00
R2209:Cry1	UTSW	10	85146755	missense	probably damaging	0.98
R2221:Cry1	UTSW	10	85143753	missense	probably damaging	1.00
R2223:Cry1	UTSW	10	85143753	missense	probably damaging	1.00
R2314:Cry1	UTSW	10	85133311	missense	probably benign	0.08
R3851:Cry1	UTSW	10	85146499	missense	probably benign	0.15
R3872:Cry1	UTSW	10	85133160	critical splice acceptor site	probably null
R3981:Cry1	UTSW	10	85146592	missense	probably damaging	0.99
R4856:Cry1	UTSW	10	85148770	missense	probably damaging	0.97
R5162:Cry1	UTSW	10	85133286	missense	probably benign
R5404:Cry1	UTSW	10	85184419	missense	probably damaging	1.00
R5449:Cry1	UTSW	10	85133135	missense	probably benign	0.17
R5484:Cry1	UTSW	10	85146724	splice site	probably null
R5599:Cry1	UTSW	10	85144250	missense	probably benign	0.14
R5717:Cry1	UTSW	10	85146416	missense	probably damaging	1.00
R7031:Cry1	UTSW	10	85148662	missense	probably benign	0.00
R7371:Cry1	UTSW	10	85147919	missense	probably benign	0.03
R7943:Cry1	UTSW	10	85143120	missense	probably benign	0.03
R8022:Cry1	UTSW	10	85146402	missense	probably damaging	0.97
R8290:Cry1	UTSW	10	85143113	nonsense	probably null
R8805:Cry1	UTSW	10	85157105	missense	probably benign	0.09
Z1176:Cry1	UTSW	10	85144197	missense	probably benign	0.00

Predicted Primers

PCR Primer
(F):5'- AAATCAGGGAAGGCGTTCCCATC -3'
(R):5'- TGAAGCTCTCGGTAGAGGAAGTCG -3'

Sequencing Primer
(F):5'- CAGGGTCGTCAATAATCTCTACTAC -3'
(R):5'- TAGAGGAAGTCGGGGTGACC -3'

Posted On

2013-07-11