Incidental Mutation 'PIT4810001:Gpr158'
| ID |
556827 |
| Institutional Source |
Beutler Lab
|
| Gene Symbol |
Gpr158
|
| Ensembl Gene |
ENSMUSG00000045967 |
| Gene Name |
G protein-coupled receptor 158 |
| Synonyms |
5330427M13Rik |
| Accession Numbers |
|
| Essential gene? |
Non essential
(E-score: 0.000)
|
| Stock # |
PIT4810001 (G1)
|
| Quality Score |
225.009 |
|
Status
|
Not validated
|
| Chromosome |
2 |
| Chromosomal Location |
21372378-21835355 bp(+) (GRCm39) |
| Type of Mutation |
missense |
| DNA Base Change (assembly) |
C to G
at 21831682 bp (GRCm39)
|
| Zygosity |
Heterozygous |
| Amino Acid Change |
Aspartic acid to Glutamic Acid
at position 927
(D927E)
|
| Ref Sequence |
ENSEMBL: ENSMUSP00000049708
(fasta)
|
| Gene Model |
predicted gene model for transcript(s):
[ENSMUST00000055946]
|
| AlphaFold |
Q8C419 |
| Predicted Effect |
probably benign
Transcript: ENSMUST00000055946
AA Change: D927E
PolyPhen 2
Score 0.006 (Sensitivity: 0.97; Specificity: 0.75)
|
| SMART Domains |
Protein: ENSMUSP00000049708
Gene: ENSMUSG00000045967
AA Change: D927E
| Domain | Start | End | E-Value | Type |
|---|
|
signal peptide
|
1 |
20 |
N/A |
INTRINSIC |
|
low complexity region
|
110 |
125 |
N/A |
INTRINSIC |
|
SCOP:d1edmb_
|
313 |
359 |
5e-4 |
SMART |
|
Blast:EGF
|
318 |
365 |
2e-27 |
BLAST |
|
Pfam:7tm_3
|
426 |
669 |
1.2e-35 |
PFAM |
|
low complexity region
|
840 |
863 |
N/A |
INTRINSIC |
|
| Coding Region Coverage |
- 1x: 93.8%
- 3x: 91.1%
- 10x: 85.1%
- 20x: 72.2%
|
| Validation Efficiency |
|
| Allele List at MGI |
|
| Other mutations in this stock |
Total: 65 list
| Gene | Ref | Var | Chr/Loc | Mutation | Predicted Effect | Zygosity |
|---|
| 4932438H23Rik |
A |
G |
16: 90,852,822 (GRCm39) |
S105P |
probably damaging |
Het |
| Adtrp |
C |
T |
13: 41,981,724 (GRCm39) |
W48* |
probably null |
Het |
| Agxt2 |
A |
T |
15: 10,399,151 (GRCm39) |
M413L |
probably benign |
Het |
| Ahnak2 |
T |
A |
12: 112,749,214 (GRCm39) |
D211V |
|
Het |
| Aplnr |
T |
A |
2: 84,967,628 (GRCm39) |
C218S |
probably damaging |
Het |
| Arhgdib |
T |
A |
6: 136,901,162 (GRCm39) |
E189V |
probably damaging |
Het |
| Atp10a |
T |
C |
7: 58,463,596 (GRCm39) |
I1043T |
probably damaging |
Het |
| Atp13a5 |
C |
T |
16: 29,133,382 (GRCm39) |
C468Y |
probably damaging |
Het |
| Cars2 |
A |
T |
8: 11,564,699 (GRCm39) |
C459S |
probably benign |
Het |
| Ccr3 |
T |
A |
9: 123,829,645 (GRCm39) |
Y327N |
probably benign |
Het |
| Ceacam1 |
T |
A |
7: 25,171,400 (GRCm39) |
I355F |
probably damaging |
Het |
| Celsr3 |
T |
C |
9: 108,722,932 (GRCm39) |
L2773P |
probably damaging |
Het |
| Ces2g |
A |
T |
8: 105,691,521 (GRCm39) |
H192L |
possibly damaging |
Het |
| Cfap157 |
A |
G |
2: 32,671,444 (GRCm39) |
F121L |
probably damaging |
Het |
| Champ1 |
G |
A |
8: 13,929,234 (GRCm39) |
S464N |
probably benign |
Het |
| Clasp2 |
T |
A |
9: 113,735,135 (GRCm39) |
L990Q |
probably damaging |
Het |
| Col6a3 |
A |
T |
1: 90,706,516 (GRCm39) |
L2806Q |
unknown |
Het |
| Cpz |
T |
C |
5: 35,665,536 (GRCm39) |
E435G |
possibly damaging |
Het |
| Crbn |
T |
A |
6: 106,761,440 (GRCm39) |
R233* |
probably null |
Het |
| Ctc1 |
C |
T |
11: 68,913,352 (GRCm39) |
T116I |
probably benign |
Het |
| Cyp4f17 |
T |
C |
17: 32,743,574 (GRCm39) |
S314P |
possibly damaging |
Het |
| Dmxl1 |
A |
G |
18: 50,065,030 (GRCm39) |
E2625G |
probably damaging |
Het |
| Dock7 |
T |
A |
4: 98,833,796 (GRCm39) |
R1874* |
probably null |
Het |
| E130311K13Rik |
A |
T |
3: 63,823,122 (GRCm39) |
L174* |
probably null |
Het |
| Etnppl |
A |
G |
3: 130,414,363 (GRCm39) |
D86G |
probably benign |
Het |
| Fbxw18 |
C |
A |
9: 109,505,958 (GRCm39) |
E438* |
probably null |
Het |
| Gpatch8 |
T |
C |
11: 102,372,668 (GRCm39) |
N290S |
unknown |
Het |
| Gpr155 |
A |
C |
2: 73,178,607 (GRCm39) |
L727R |
probably benign |
Het |
| Igsf10 |
T |
C |
3: 59,225,903 (GRCm39) |
Y2590C |
probably damaging |
Het |
| Ints7 |
A |
G |
1: 191,328,348 (GRCm39) |
D207G |
probably damaging |
Het |
| Iqsec1 |
A |
G |
6: 90,647,473 (GRCm39) |
L743P |
probably damaging |
Het |
| Kansl1l |
A |
T |
1: 66,801,308 (GRCm39) |
S553T |
probably damaging |
Het |
| Klhl14 |
G |
T |
18: 21,690,880 (GRCm39) |
Y523* |
probably null |
Het |
| Krtap14 |
A |
G |
16: 88,622,515 (GRCm39) |
S155P |
probably damaging |
Het |
| Lrrc3b |
A |
G |
14: 15,358,273 (GRCm38) |
V111A |
probably benign |
Het |
| Maml2 |
A |
G |
9: 13,531,320 (GRCm39) |
N178S |
|
Het |
| Megf8 |
G |
A |
7: 25,041,710 (GRCm39) |
C1208Y |
probably damaging |
Het |
| Mocos |
A |
G |
18: 24,819,759 (GRCm39) |
D667G |
probably damaging |
Het |
| Nid2 |
A |
G |
14: 19,860,158 (GRCm39) |
T1359A |
possibly damaging |
Het |
| Nup210 |
T |
C |
6: 91,007,106 (GRCm39) |
E1357G |
probably damaging |
Het |
| Or2d2 |
A |
T |
7: 106,727,766 (GRCm39) |
M278K |
probably benign |
Het |
| Or4a2 |
A |
T |
2: 89,248,297 (GRCm39) |
H153Q |
probably damaging |
Het |
| Or9s15 |
T |
A |
1: 92,524,876 (GRCm39) |
F212I |
probably benign |
Het |
| Pign |
A |
G |
1: 105,525,487 (GRCm39) |
V447A |
possibly damaging |
Het |
| Pomp |
T |
C |
5: 147,806,229 (GRCm39) |
L57P |
probably benign |
Het |
| Pou2af2 |
C |
T |
9: 51,202,992 (GRCm39) |
G54D |
possibly damaging |
Het |
| Rbms3 |
T |
A |
9: 116,885,861 (GRCm39) |
Y128F |
probably damaging |
Het |
| Slc12a4 |
A |
G |
8: 106,678,228 (GRCm39) |
F322L |
probably benign |
Het |
| Slc16a9 |
T |
A |
10: 70,119,762 (GRCm39) |
C468* |
probably null |
Het |
| Slc22a18 |
A |
G |
7: 143,046,668 (GRCm39) |
I271V |
probably benign |
Het |
| Slc25a41 |
A |
G |
17: 57,346,933 (GRCm39) |
V20A |
possibly damaging |
Het |
| Smim7 |
A |
T |
8: 73,324,857 (GRCm39) |
L6Q |
probably damaging |
Het |
| Snx29 |
T |
C |
16: 11,218,845 (GRCm39) |
V227A |
probably damaging |
Het |
| Sowaha |
A |
T |
11: 53,369,290 (GRCm39) |
L482Q |
probably damaging |
Het |
| Srp19 |
A |
G |
18: 34,467,523 (GRCm39) |
Y68C |
probably damaging |
Het |
| Tecr |
A |
G |
8: 84,298,884 (GRCm39) |
S298P |
probably damaging |
Het |
| Terb2 |
T |
A |
2: 122,035,379 (GRCm39) |
I200N |
probably damaging |
Het |
| Togaram1 |
G |
A |
12: 65,030,286 (GRCm39) |
S1030N |
probably damaging |
Het |
| Trav7n-4 |
A |
G |
14: 53,329,193 (GRCm39) |
M68V |
probably benign |
Het |
| Tshz1 |
A |
C |
18: 84,031,375 (GRCm39) |
L1011R |
possibly damaging |
Het |
| Tubb3 |
A |
G |
8: 124,148,396 (GRCm39) |
E443G |
possibly damaging |
Het |
| Uba5 |
A |
T |
9: 103,932,396 (GRCm39) |
I189K |
probably damaging |
Het |
| Vasn |
A |
G |
16: 4,467,909 (GRCm39) |
T619A |
probably benign |
Het |
| Wbp1l |
A |
G |
19: 46,642,761 (GRCm39) |
D254G |
probably benign |
Het |
| Zzef1 |
T |
C |
11: 72,741,571 (GRCm39) |
F738L |
probably damaging |
Het |
|
| Other mutations in Gpr158 |
| Allele | Source | Chr | Coord | Type | Predicted Effect | PPH Score |
|---|
|
IGL00340:Gpr158
|
APN |
2 |
21,373,494 (GRCm39) |
missense |
probably damaging |
1.00 |
|
IGL00469:Gpr158
|
APN |
2 |
21,751,606 (GRCm39) |
splice site |
probably benign |
|
|
IGL00706:Gpr158
|
APN |
2 |
21,751,584 (GRCm39) |
missense |
probably damaging |
1.00 |
|
IGL00780:Gpr158
|
APN |
2 |
21,831,629 (GRCm39) |
nonsense |
probably null |
|
|
IGL00885:Gpr158
|
APN |
2 |
21,653,832 (GRCm39) |
missense |
probably damaging |
1.00 |
|
IGL01339:Gpr158
|
APN |
2 |
21,373,842 (GRCm39) |
missense |
possibly damaging |
0.73 |
|
IGL01368:Gpr158
|
APN |
2 |
21,831,909 (GRCm39) |
missense |
probably damaging |
1.00 |
|
IGL02141:Gpr158
|
APN |
2 |
21,788,101 (GRCm39) |
missense |
probably damaging |
0.99 |
|
IGL02455:Gpr158
|
APN |
2 |
21,373,511 (GRCm39) |
missense |
probably benign |
0.00 |
|
IGL02554:Gpr158
|
APN |
2 |
21,831,407 (GRCm39) |
missense |
probably benign |
|
|
IGL02681:Gpr158
|
APN |
2 |
21,820,441 (GRCm39) |
missense |
probably damaging |
1.00 |
|
IGL02752:Gpr158
|
APN |
2 |
21,831,638 (GRCm39) |
missense |
possibly damaging |
0.95 |
|
IGL02756:Gpr158
|
APN |
2 |
21,831,890 (GRCm39) |
missense |
possibly damaging |
0.47 |
|
IGL03181:Gpr158
|
APN |
2 |
21,787,972 (GRCm39) |
missense |
probably benign |
0.02 |
|
IGL03258:Gpr158
|
APN |
2 |
21,830,085 (GRCm39) |
missense |
probably damaging |
1.00 |
|
IGL03386:Gpr158
|
APN |
2 |
21,831,057 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R0071:Gpr158
|
UTSW |
2 |
21,815,479 (GRCm39) |
missense |
probably benign |
0.08 |
|
R0081:Gpr158
|
UTSW |
2 |
21,831,528 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R0528:Gpr158
|
UTSW |
2 |
21,830,019 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R0560:Gpr158
|
UTSW |
2 |
21,830,085 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R0603:Gpr158
|
UTSW |
2 |
21,820,480 (GRCm39) |
missense |
possibly damaging |
0.67 |
|
R1560:Gpr158
|
UTSW |
2 |
21,831,125 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R1561:Gpr158
|
UTSW |
2 |
21,820,505 (GRCm39) |
splice site |
probably null |
|
|
R1609:Gpr158
|
UTSW |
2 |
21,788,104 (GRCm39) |
missense |
possibly damaging |
0.61 |
|
R1741:Gpr158
|
UTSW |
2 |
21,832,359 (GRCm39) |
missense |
probably benign |
0.00 |
|
R1827:Gpr158
|
UTSW |
2 |
21,832,129 (GRCm39) |
missense |
probably benign |
|
|
R1854:Gpr158
|
UTSW |
2 |
21,373,935 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R1871:Gpr158
|
UTSW |
2 |
21,820,426 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R2151:Gpr158
|
UTSW |
2 |
21,832,325 (GRCm39) |
missense |
possibly damaging |
0.82 |
|
R2273:Gpr158
|
UTSW |
2 |
21,831,674 (GRCm39) |
missense |
probably benign |
|
|
R2275:Gpr158
|
UTSW |
2 |
21,831,674 (GRCm39) |
missense |
probably benign |
|
|
R3004:Gpr158
|
UTSW |
2 |
21,831,810 (GRCm39) |
missense |
probably damaging |
0.99 |
|
R3151:Gpr158
|
UTSW |
2 |
21,581,771 (GRCm39) |
missense |
possibly damaging |
0.68 |
|
R3943:Gpr158
|
UTSW |
2 |
21,373,370 (GRCm39) |
missense |
possibly damaging |
0.65 |
|
R4238:Gpr158
|
UTSW |
2 |
21,373,362 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R4379:Gpr158
|
UTSW |
2 |
21,830,025 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R4381:Gpr158
|
UTSW |
2 |
21,832,403 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R4464:Gpr158
|
UTSW |
2 |
21,831,810 (GRCm39) |
missense |
probably damaging |
0.99 |
|
R4467:Gpr158
|
UTSW |
2 |
21,831,810 (GRCm39) |
missense |
probably damaging |
0.99 |
|
R4496:Gpr158
|
UTSW |
2 |
21,831,810 (GRCm39) |
missense |
probably damaging |
0.99 |
|
R4506:Gpr158
|
UTSW |
2 |
21,831,810 (GRCm39) |
missense |
probably damaging |
0.99 |
|
R4530:Gpr158
|
UTSW |
2 |
21,373,811 (GRCm39) |
missense |
probably benign |
0.03 |
|
R4646:Gpr158
|
UTSW |
2 |
21,831,864 (GRCm39) |
missense |
probably benign |
|
|
R4798:Gpr158
|
UTSW |
2 |
21,787,993 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R4882:Gpr158
|
UTSW |
2 |
21,830,059 (GRCm39) |
missense |
probably damaging |
0.98 |
|
R4943:Gpr158
|
UTSW |
2 |
21,831,968 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R5334:Gpr158
|
UTSW |
2 |
21,832,316 (GRCm39) |
missense |
probably benign |
0.01 |
|
R5560:Gpr158
|
UTSW |
2 |
21,831,101 (GRCm39) |
missense |
possibly damaging |
0.67 |
|
R5600:Gpr158
|
UTSW |
2 |
21,832,046 (GRCm39) |
missense |
probably benign |
|
|
R5637:Gpr158
|
UTSW |
2 |
21,788,083 (GRCm39) |
missense |
probably benign |
0.00 |
|
R5701:Gpr158
|
UTSW |
2 |
21,751,520 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R5744:Gpr158
|
UTSW |
2 |
21,373,331 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R5911:Gpr158
|
UTSW |
2 |
21,373,932 (GRCm39) |
missense |
possibly damaging |
0.95 |
|
R5991:Gpr158
|
UTSW |
2 |
21,373,319 (GRCm39) |
missense |
probably damaging |
0.99 |
|
R6200:Gpr158
|
UTSW |
2 |
21,404,227 (GRCm39) |
missense |
probably damaging |
0.97 |
|
R6306:Gpr158
|
UTSW |
2 |
21,820,422 (GRCm39) |
missense |
possibly damaging |
0.84 |
|
R6324:Gpr158
|
UTSW |
2 |
21,815,365 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R6384:Gpr158
|
UTSW |
2 |
21,831,099 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R6698:Gpr158
|
UTSW |
2 |
21,831,921 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R6997:Gpr158
|
UTSW |
2 |
21,653,802 (GRCm39) |
missense |
possibly damaging |
0.46 |
|
R7086:Gpr158
|
UTSW |
2 |
21,831,386 (GRCm39) |
missense |
probably benign |
0.01 |
|
R7175:Gpr158
|
UTSW |
2 |
21,373,113 (GRCm39) |
missense |
probably benign |
0.13 |
|
R7197:Gpr158
|
UTSW |
2 |
21,815,412 (GRCm39) |
missense |
probably damaging |
0.99 |
|
R7293:Gpr158
|
UTSW |
2 |
21,581,750 (GRCm39) |
missense |
possibly damaging |
0.47 |
|
R7427:Gpr158
|
UTSW |
2 |
21,832,129 (GRCm39) |
missense |
probably benign |
|
|
R7515:Gpr158
|
UTSW |
2 |
21,373,092 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R7730:Gpr158
|
UTSW |
2 |
21,831,158 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R8122:Gpr158
|
UTSW |
2 |
21,831,674 (GRCm39) |
missense |
probably benign |
|
|
R8311:Gpr158
|
UTSW |
2 |
21,373,701 (GRCm39) |
missense |
probably benign |
0.00 |
|
R8754:Gpr158
|
UTSW |
2 |
21,581,693 (GRCm39) |
missense |
probably benign |
0.00 |
|
R8782:Gpr158
|
UTSW |
2 |
21,404,149 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R8792:Gpr158
|
UTSW |
2 |
21,558,137 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R8842:Gpr158
|
UTSW |
2 |
21,581,751 (GRCm39) |
missense |
possibly damaging |
0.88 |
|
R9009:Gpr158
|
UTSW |
2 |
21,581,760 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R9102:Gpr158
|
UTSW |
2 |
21,830,078 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R9150:Gpr158
|
UTSW |
2 |
21,831,251 (GRCm39) |
missense |
probably benign |
0.17 |
|
R9254:Gpr158
|
UTSW |
2 |
21,373,042 (GRCm39) |
start gained |
probably benign |
|
|
R9317:Gpr158
|
UTSW |
2 |
21,832,037 (GRCm39) |
missense |
probably benign |
|
|
R9379:Gpr158
|
UTSW |
2 |
21,373,042 (GRCm39) |
start gained |
probably benign |
|
|
R9428:Gpr158
|
UTSW |
2 |
21,787,972 (GRCm39) |
missense |
probably benign |
|
|
R9497:Gpr158
|
UTSW |
2 |
21,831,825 (GRCm39) |
missense |
probably benign |
0.00 |
|
R9667:Gpr158
|
UTSW |
2 |
21,830,054 (GRCm39) |
missense |
probably damaging |
0.99 |
|
R9681:Gpr158
|
UTSW |
2 |
21,831,315 (GRCm39) |
missense |
probably damaging |
0.99 |
|
X0062:Gpr158
|
UTSW |
2 |
21,831,180 (GRCm39) |
missense |
probably damaging |
1.00 |
|
Z1176:Gpr158
|
UTSW |
2 |
21,815,501 (GRCm39) |
critical splice donor site |
probably null |
|
|
Z1177:Gpr158
|
UTSW |
2 |
21,832,083 (GRCm39) |
missense |
possibly damaging |
0.46 |
|
| Predicted Primers |
PCR Primer
(F):5'- GAAAATTCAACCTTGGAGTCGC -3'
(R):5'- TCACGTCAGGATTGGCAGTG -3'
Sequencing Primer
(F):5'- TCAACCTTGGAGTCGCTGTCAAG -3'
(R):5'- TCAGGATTGGCAGTGGGGAG -3'
|
| Posted On |
2019-06-07 |
Incidental Mutation