Incidental Mutation 'PIT4791001:Cnr2'
ID 556964
Institutional Source Beutler Lab
Gene Symbol Cnr2
Ensembl Gene ENSMUSG00000062585
Gene Name cannabinoid receptor 2
Synonyms cannabinoid receptor 2 (spleen), CB2-R, CB2
Accession Numbers
Essential gene? Probably non essential (E-score: 0.072) question?
Stock # PIT4791001 (G1)
Quality Score 225.009
Status Not validated
Chromosome 4
Chromosomal Location 135622705-135647518 bp(+) (GRCm39)
Type of Mutation missense
DNA Base Change (assembly) A to G at 135644263 bp (GRCm39)
Zygosity Heterozygous
Amino Acid Change Threonine to Alanine at position 114 (T114A)
Ref Sequence ENSEMBL: ENSMUSP00000069957 (fasta)
Gene Model predicted gene model for transcript(s): [ENSMUST00000030434] [ENSMUST00000068830] [ENSMUST00000097843]
AlphaFold P47936
Predicted Effect probably benign
Transcript: ENSMUST00000030434
SMART Domains Protein: ENSMUSP00000030434
Gene: ENSMUSG00000028673

DomainStartEndE-ValueType
low complexity region 2 19 N/A INTRINSIC
Alpha_L_fucos 22 399 7.97e-234 SMART
Predicted Effect probably damaging
Transcript: ENSMUST00000068830
AA Change: T114A

PolyPhen 2 Score 0.997 (Sensitivity: 0.41; Specificity: 0.98)
SMART Domains Protein: ENSMUSP00000069957
Gene: ENSMUSG00000062585
AA Change: T114A

DomainStartEndE-ValueType
Pfam:7tm_1 50 299 1.8e-44 PFAM
Predicted Effect probably damaging
Transcript: ENSMUST00000097843
AA Change: T114A

PolyPhen 2 Score 0.997 (Sensitivity: 0.41; Specificity: 0.98)
SMART Domains Protein: ENSMUSP00000095454
Gene: ENSMUSG00000062585
AA Change: T114A

DomainStartEndE-ValueType
Pfam:7tm_1 50 299 8e-37 PFAM
Coding Region Coverage
  • 1x: 93.5%
  • 3x: 90.8%
  • 10x: 83.3%
  • 20x: 68.6%
Validation Efficiency
MGI Phenotype FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] The cannabinoid delta-9-tetrahydrocannabinol is the principal psychoactive ingredient of marijuana. The proteins encoded by this gene and the cannabinoid receptor 1 (brain) (CNR1) gene have the characteristics of a guanine nucleotide-binding protein (G-protein)-coupled receptor for cannabinoids. They inhibit adenylate cyclase activity in a dose-dependent, stereoselective, and pertussis toxin-sensitive manner. These proteins have been found to be involved in the cannabinoid-induced CNS effects (including alterations in mood and cognition) experienced by users of marijuana. The cannabinoid receptors are members of family 1 of the G-protein-coupled receptors. [provided by RefSeq, Jul 2008]
PHENOTYPE: Macrophages from homozygous mutant animals are resistant to the inhibitory effects of delta9-Tetrahydrocannabinol. Alopecia is seen in some but not all homozygotes. [provided by MGI curators]
Allele List at MGI
Other mutations in this stock
Total: 44 list
GeneRefVarChr/LocMutationPredicted EffectZygosity
Ago2 G A 15: 72,993,089 (GRCm39) T472I possibly damaging Het
Ambp T A 4: 63,072,298 (GRCm39) probably benign Het
As3mt T A 19: 46,708,788 (GRCm39) F261I probably damaging Het
Atxn1l A G 8: 110,458,579 (GRCm39) L561P probably damaging Het
Dchs1 A G 7: 105,408,178 (GRCm39) Y1885H probably damaging Het
Dcn A G 10: 97,343,604 (GRCm39) N188S probably benign Het
Dennd6b A C 15: 89,070,955 (GRCm39) probably null Het
Dstyk A G 1: 132,377,665 (GRCm39) T424A probably benign Het
Gabrr3 A G 16: 59,250,298 (GRCm39) probably null Het
Gnai3 A G 3: 108,025,621 (GRCm39) S153P probably benign Het
Igkv2-112 T A 6: 68,197,599 (GRCm39) S90R probably damaging Het
Lxn T C 3: 67,365,979 (GRCm39) D173G probably damaging Het
Mcm3ap T A 10: 76,342,307 (GRCm39) I1600N probably damaging Het
Myof A T 19: 37,971,406 (GRCm39) probably null Het
Nrxn1 T C 17: 90,762,931 (GRCm39) probably benign Het
Oaz3 A G 3: 94,340,852 (GRCm39) Y232H unknown Het
Or5aq1b A T 2: 86,902,046 (GRCm39) M144K possibly damaging Het
Pkdcc C T 17: 83,527,577 (GRCm39) R252* probably null Het
Plcl1 A G 1: 55,741,090 (GRCm39) N907S probably benign Het
Plscr1 T A 9: 92,145,150 (GRCm39) Y53* probably null Het
Pm20d2 T C 4: 33,174,756 (GRCm39) T377A probably benign Het
Ptchd3 T A 11: 121,722,875 (GRCm39) V321D probably damaging Het
Ranbp3l T C 15: 9,060,829 (GRCm39) I366T probably damaging Het
Sardh T C 2: 27,087,660 (GRCm39) E737G probably damaging Het
Scart2 T C 7: 139,853,975 (GRCm39) F326S possibly damaging Het
Scn1a T C 2: 66,103,626 (GRCm39) E1878G probably benign Het
Slit3 T C 11: 35,552,072 (GRCm39) V872A possibly damaging Het
Sugp2 C T 8: 70,713,195 (GRCm39) Q1054* probably null Het
Syne3 T C 12: 104,929,438 (GRCm39) T261A probably benign Het
Tgif2 T C 2: 156,695,232 (GRCm39) S126P probably benign Het
Ticrr T C 7: 79,319,386 (GRCm39) V457A possibly damaging Het
Tm7sf2 T C 19: 6,113,635 (GRCm39) I299V probably benign Het
Tnks1bp1 A G 2: 84,892,902 (GRCm39) E943G probably benign Het
Tpd52l2 T A 2: 181,141,681 (GRCm39) F21L probably benign Het
Tpm2 A G 4: 43,519,263 (GRCm39) L176P probably benign Het
Trim34a C A 7: 103,909,691 (GRCm39) T293K probably benign Het
Ttc23 A T 7: 67,312,135 (GRCm39) D14V probably damaging Het
Twsg1 T C 17: 66,236,706 (GRCm39) E108G probably benign Het
Txndc15 C T 13: 55,869,507 (GRCm39) A220V probably benign Het
Vmn1r183 A G 7: 23,754,266 (GRCm39) Q23R probably damaging Het
Vmn1r31 A G 6: 58,449,028 (GRCm39) V279A probably damaging Het
Vmo1 A C 11: 70,404,661 (GRCm39) C113W probably damaging Het
Zfp984 A G 4: 147,840,603 (GRCm39) S83P probably benign Het
Zik1 A C 7: 10,226,256 (GRCm39) L37R probably benign Het
Other mutations in Cnr2
AlleleSourceChrCoordTypePredicted EffectPPH Score
IGL01990:Cnr2 APN 4 135,644,116 (GRCm39) missense probably damaging 1.00
IGL02233:Cnr2 APN 4 135,644,522 (GRCm39) missense possibly damaging 0.76
IGL02887:Cnr2 APN 4 135,644,936 (GRCm39) missense possibly damaging 0.90
R0480:Cnr2 UTSW 4 135,644,912 (GRCm39) missense probably benign 0.00
R0616:Cnr2 UTSW 4 135,644,873 (GRCm39) missense probably benign 0.39
R0945:Cnr2 UTSW 4 135,644,632 (GRCm39) missense probably benign 0.16
R1242:Cnr2 UTSW 4 135,644,294 (GRCm39) missense probably damaging 1.00
R1538:Cnr2 UTSW 4 135,644,012 (GRCm39) missense probably benign 0.00
R4330:Cnr2 UTSW 4 135,644,237 (GRCm39) missense possibly damaging 0.56
R4911:Cnr2 UTSW 4 135,644,512 (GRCm39) missense possibly damaging 0.88
R4960:Cnr2 UTSW 4 135,644,918 (GRCm39) missense probably benign 0.01
R5289:Cnr2 UTSW 4 135,644,318 (GRCm39) missense probably damaging 1.00
R5642:Cnr2 UTSW 4 135,644,076 (GRCm39) missense probably damaging 1.00
R5681:Cnr2 UTSW 4 135,644,000 (GRCm39) missense probably damaging 0.99
R5807:Cnr2 UTSW 4 135,644,747 (GRCm39) missense probably benign 0.00
R6843:Cnr2 UTSW 4 135,644,900 (GRCm39) missense probably benign
R8024:Cnr2 UTSW 4 135,644,196 (GRCm39) missense probably damaging 1.00
R8423:Cnr2 UTSW 4 135,644,827 (GRCm39) missense probably damaging 1.00
R9330:Cnr2 UTSW 4 135,644,312 (GRCm39) missense probably damaging 1.00
Predicted Primers PCR Primer
(F):5'- GGCCGTGCTCTATATTATCCTG -3'
(R):5'- CAGCCCAGTAGGTAGTCGTTAG -3'

Sequencing Primer
(F):5'- ATATTATCCTGTCCTCCCGGCG -3'
(R):5'- CCCAGTAGGTAGTCGTTAGGGATC -3'
Posted On 2019-06-07