Incidental Mutation 'R7155:Slc22a3'
ID 557264
Institutional Source Beutler Lab
Gene Symbol Slc22a3
Ensembl Gene ENSMUSG00000023828
Gene Name solute carrier family 22 (organic cation transporter), member 3
Synonyms EMT, Oct3, Orct3
MMRRC Submission 045226-MU
Accession Numbers
Essential gene? Non essential (E-score: 0.000) question?
Stock # R7155 (G1)
Quality Score 225.009
Status Not validated
Chromosome 17
Chromosomal Location 12638859-12726591 bp(-) (GRCm39)
Type of Mutation missense
DNA Base Change (assembly) G to A at 12652518 bp (GRCm39)
Zygosity Heterozygous
Amino Acid Change Leucine to Phenylalanine at position 369 (L369F)
Ref Sequence ENSEMBL: ENSMUSP00000024595 (fasta)
Gene Model predicted gene model for transcript(s): [ENSMUST00000024595]
AlphaFold Q9WTW5
Predicted Effect possibly damaging
Transcript: ENSMUST00000024595
AA Change: L369F

PolyPhen 2 Score 0.829 (Sensitivity: 0.84; Specificity: 0.93)
SMART Domains Protein: ENSMUSP00000024595
Gene: ENSMUSG00000023828
AA Change: L369F

DomainStartEndE-ValueType
transmembrane domain 21 43 N/A INTRINSIC
Pfam:Sugar_tr 105 526 1.2e-28 PFAM
Pfam:MFS_1 144 395 3.3e-22 PFAM
Coding Region Coverage
  • 1x: 100.0%
  • 3x: 99.9%
  • 10x: 99.7%
  • 20x: 98.8%
Validation Efficiency
MGI Phenotype FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] Polyspecific organic cation transporters in the liver, kidney, intestine, and other organs are critical for elimination of many endogenous small organic cations as well as a wide array of drugs and environmental toxins. This gene is one of three similar cation transporter genes located in a cluster on chromosome 6. The encoded protein contains twelve putative transmembrane domains and is a plasma integral membrane protein. [provided by RefSeq, Jul 2008]
PHENOTYPE: Mice homozygous for a knock-out allele exhibit increased susceptibility to paraquat-induced dopamine neuron neurotoxicity. [provided by MGI curators]
Allele List at MGI
Other mutations in this stock
Total: 85 list
GeneRefVarChr/LocMutationPredicted EffectZygosity
4921509C19Rik A G 2: 151,315,489 (GRCm39) L63P possibly damaging Het
Abca13 A T 11: 9,479,010 (GRCm39) Y4286F probably benign Het
Aldh5a1 A C 13: 25,095,572 (GRCm39) V515G possibly damaging Het
Ankrd42 T C 7: 92,241,141 (GRCm39) E406G possibly damaging Het
Arfgef2 T A 2: 166,707,733 (GRCm39) M1043K probably benign Het
Arhgef11 T A 3: 87,616,879 (GRCm39) Y383* probably null Het
Aste1 C T 9: 105,282,335 (GRCm39) P614L probably damaging Het
B3galt5 T A 16: 96,117,005 (GRCm39) S213T probably damaging Het
Bak1 A G 17: 27,241,434 (GRCm39) L108P possibly damaging Het
Bcl6 G A 16: 23,784,976 (GRCm39) R675* probably null Het
Bdp1 T G 13: 100,197,659 (GRCm39) T909P possibly damaging Het
Cacna1i A G 15: 80,279,439 (GRCm39) H2060R probably benign Het
Cdhr3 G T 12: 33,111,772 (GRCm39) P246Q probably damaging Het
Colgalt1 T C 8: 72,076,354 (GRCm39) S602P probably damaging Het
Csde1 T C 3: 102,947,269 (GRCm39) S74P probably damaging Het
Cyp51 A T 5: 4,137,846 (GRCm39) C366S possibly damaging Het
Dcaf7 A G 11: 105,928,016 (GRCm39) N23D probably damaging Het
Ddx10 G A 9: 53,028,588 (GRCm39) A772V probably benign Het
Ddx4 A G 13: 112,750,319 (GRCm39) F404S probably benign Het
Dlg4 T A 11: 69,908,042 (GRCm39) M1K probably null Het
Dnajc6 T C 4: 101,470,142 (GRCm39) V293A probably damaging Het
Etl4 G T 2: 20,811,742 (GRCm39) R1643L probably damaging Het
F13b G A 1: 139,435,895 (GRCm39) E234K probably damaging Het
Fam171a1 T C 2: 3,226,766 (GRCm39) I633T probably benign Het
Frem3 A G 8: 81,342,668 (GRCm39) I1654V probably benign Het
Galr2 A G 11: 116,174,408 (GRCm39) E346G possibly damaging Het
Gck G A 11: 5,899,705 (GRCm39) probably benign Het
Gen1 G T 12: 11,291,833 (GRCm39) T717K probably benign Het
Gpatch8 TTCCTCCTCCTCCTCTTCCTCCTCCTC TTCCTCCTCCTCCTCCTCTTCCTCCTCCTC 11: 102,371,014 (GRCm39) probably benign Het
Hat1 A G 2: 71,251,595 (GRCm39) T215A possibly damaging Het
Hmbox1 A T 14: 65,134,486 (GRCm39) M38K probably damaging Het
Ifi47 A G 11: 48,987,369 (GRCm39) K379E probably benign Het
Ift81 T C 5: 122,707,062 (GRCm39) Y460C probably damaging Het
Jarid2 T A 13: 45,055,938 (GRCm39) S381R probably damaging Het
Kmt2b A T 7: 30,279,388 (GRCm39) V1458E probably damaging Het
Kpna4 G T 3: 68,997,266 (GRCm39) P336Q probably damaging Het
Krt84 T C 15: 101,440,689 (GRCm39) R168G probably damaging Het
Lhx8 T A 3: 154,030,221 (GRCm39) Y137F possibly damaging Het
Lin7b T C 7: 45,019,651 (GRCm39) E19G probably damaging Het
Lrmda A T 14: 22,634,608 (GRCm39) R131S probably damaging Het
Lrrc75b C T 10: 75,389,512 (GRCm39) A280T possibly damaging Het
Megf11 G A 9: 64,555,233 (GRCm39) R268K probably null Het
Mgat4e G T 1: 134,469,697 (GRCm39) Q116K probably damaging Het
Mlkl A G 8: 112,046,035 (GRCm39) L325P probably damaging Het
Mup2 A G 4: 60,137,641 (GRCm39) L134P probably damaging Het
Myo19 A G 11: 84,791,412 (GRCm39) E489G probably damaging Het
Ncbp3 C A 11: 72,938,835 (GRCm39) P37Q probably damaging Het
Nf2 A G 11: 4,749,964 (GRCm39) V236A probably damaging Het
Nlrp1a A T 11: 71,014,905 (GRCm39) M115K possibly damaging Het
Nsf T A 11: 103,719,356 (GRCm39) K649* probably null Het
Olfml2b A T 1: 170,494,354 (GRCm39) I313L probably benign Het
Or2ak4 T A 11: 58,649,109 (GRCm39) I206N probably damaging Het
Or52u1 T A 7: 104,237,764 (GRCm39) V251D possibly damaging Het
Papln T A 12: 83,823,295 (GRCm39) L444Q probably damaging Het
Phc3 T C 3: 30,968,346 (GRCm39) I897V probably benign Het
Plcg1 T C 2: 160,596,300 (GRCm39) L632P probably damaging Het
Pramel21 A T 4: 143,342,735 (GRCm39) I281F probably benign Het
Prkg1 T C 19: 31,279,701 (GRCm39) T178A probably damaging Het
Ptges T A 2: 30,782,816 (GRCm39) T79S probably benign Het
Rab26 T C 17: 24,751,263 (GRCm39) T81A probably damaging Het
Rai14 T A 15: 10,595,089 (GRCm39) I145L possibly damaging Het
Rasgrf1 A G 9: 89,884,414 (GRCm39) T960A possibly damaging Het
Rfx1 A T 8: 84,821,455 (GRCm39) I755F probably damaging Het
Rims1 T G 1: 22,503,174 (GRCm39) L670F probably damaging Het
Rtkn G A 6: 83,126,692 (GRCm39) C297Y probably damaging Het
Sec23a A T 12: 59,036,229 (GRCm39) N378K probably benign Het
Slc1a2 T C 2: 102,597,340 (GRCm39) M449T probably damaging Het
Slc34a1 A G 13: 24,006,390 (GRCm39) E472G probably benign Het
Slc49a4 G T 16: 35,555,947 (GRCm39) T171K probably benign Het
Smad6 G T 9: 63,929,069 (GRCm39) D82E unknown Het
Smgc A T 15: 91,736,811 (GRCm39) I463F possibly damaging Het
Strada C A 11: 106,061,865 (GRCm39) G166C probably damaging Het
Tcaf3 A G 6: 42,570,825 (GRCm39) V309A probably benign Het
Tet2 C T 3: 133,175,352 (GRCm39) E1332K possibly damaging Het
Tfcp2l1 A G 1: 118,596,362 (GRCm39) N366D probably damaging Het
Tll2 G A 19: 41,105,723 (GRCm39) P369L possibly damaging Het
Tox4 T C 14: 52,529,554 (GRCm39) V505A probably benign Het
Trbv4 A G 6: 41,036,787 (GRCm39) D104G probably damaging Het
Ugdh T C 5: 65,574,380 (GRCm39) E416G probably damaging Het
Usp47 T A 7: 111,686,220 (GRCm39) C613S probably damaging Het
Vmn1r11 T A 6: 57,115,147 (GRCm39) N270K probably benign Het
Wsb2 T A 5: 117,509,160 (GRCm39) L147Q probably damaging Het
Xrn1 G A 9: 95,861,198 (GRCm39) A453T possibly damaging Het
Zan A G 5: 137,460,106 (GRCm39) S1262P unknown Het
Zswim4 A G 8: 84,946,556 (GRCm39) L700P probably damaging Het
Other mutations in Slc22a3
AlleleSourceChrCoordTypePredicted EffectPPH Score
IGL00960:Slc22a3 APN 17 12,644,497 (GRCm39) missense probably damaging 1.00
IGL01343:Slc22a3 APN 17 12,644,516 (GRCm39) missense probably damaging 1.00
IGL01621:Slc22a3 APN 17 12,677,379 (GRCm39) missense probably benign 0.02
IGL02228:Slc22a3 APN 17 12,678,697 (GRCm39) missense probably damaging 1.00
R0466:Slc22a3 UTSW 17 12,677,380 (GRCm39) nonsense probably null
R1501:Slc22a3 UTSW 17 12,725,991 (GRCm39) missense probably benign 0.12
R1688:Slc22a3 UTSW 17 12,652,694 (GRCm39) missense probably damaging 1.00
R3030:Slc22a3 UTSW 17 12,676,521 (GRCm39) missense probably benign 0.00
R4604:Slc22a3 UTSW 17 12,678,658 (GRCm39) missense probably benign 0.38
R4754:Slc22a3 UTSW 17 12,726,082 (GRCm39) missense probably benign 0.03
R4796:Slc22a3 UTSW 17 12,642,675 (GRCm39) missense probably damaging 1.00
R4865:Slc22a3 UTSW 17 12,683,419 (GRCm39) missense probably benign 0.03
R5433:Slc22a3 UTSW 17 12,677,377 (GRCm39) missense probably damaging 1.00
R5483:Slc22a3 UTSW 17 12,683,354 (GRCm39) missense probably damaging 0.99
R5719:Slc22a3 UTSW 17 12,642,691 (GRCm39) missense probably damaging 1.00
R5750:Slc22a3 UTSW 17 12,652,395 (GRCm39) missense probably benign 0.01
R5872:Slc22a3 UTSW 17 12,652,355 (GRCm39) missense probably damaging 1.00
R5988:Slc22a3 UTSW 17 12,652,782 (GRCm39) missense possibly damaging 0.92
R6197:Slc22a3 UTSW 17 12,677,438 (GRCm39) missense probably benign 0.00
R7764:Slc22a3 UTSW 17 12,677,383 (GRCm39) missense probably damaging 1.00
R7775:Slc22a3 UTSW 17 12,683,350 (GRCm39) missense probably damaging 1.00
R7824:Slc22a3 UTSW 17 12,683,350 (GRCm39) missense probably damaging 1.00
R8098:Slc22a3 UTSW 17 12,642,619 (GRCm39) critical splice donor site probably null
R8407:Slc22a3 UTSW 17 12,640,368 (GRCm39) missense probably benign 0.08
R9135:Slc22a3 UTSW 17 12,645,619 (GRCm39) missense possibly damaging 0.92
R9251:Slc22a3 UTSW 17 12,726,093 (GRCm39) missense probably damaging 1.00
R9515:Slc22a3 UTSW 17 12,726,057 (GRCm39) missense probably damaging 0.99
X0027:Slc22a3 UTSW 17 12,677,358 (GRCm39) missense possibly damaging 0.91
Z1088:Slc22a3 UTSW 17 12,644,568 (GRCm39) nonsense probably null
Z1177:Slc22a3 UTSW 17 12,726,062 (GRCm39) missense probably damaging 1.00
Z1177:Slc22a3 UTSW 17 12,726,058 (GRCm39) missense probably damaging 1.00
Z1177:Slc22a3 UTSW 17 12,725,945 (GRCm39) missense probably benign
Predicted Primers PCR Primer
(F):5'- GCTCAGAGAGACAAGTGACC -3'
(R):5'- CTTGGTAAGTTGACTCGGGC -3'

Sequencing Primer
(F):5'- CTTTGGGAACTGTCAACTAAATGTG -3'
(R):5'- CTCGGGCTGGAATTCAAAGCTG -3'
Posted On 2019-06-26