Mutagenetix > Incidental Mutation

Incidental Mutation 'R7157:Tnn'

557349

Institutional Source

Beutler Lab

Gene Symbol

Tnn

Ensembl Gene

ENSMUSG00000026725

Gene Name

tenascin N

Synonyms

Tnw, tenascin-W

MMRRC Submission

045258-MU

Accession Numbers

Genbank: NM_177839.3; Ensembl: ENSMUST00000039178

Essential gene?

Possibly non essential (E-score: 0.470) question?

Stock #

R7157 (G1)

Quality Score

225.009

Status

Not validated

Chromosome

Chromosomal Location

160085029-160153580 bp(-) (GRCm38)

Type of Mutation

nonsense

DNA Base Change (assembly)

T to A at 160126377 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Lysine to Stop codon at position 603 (K603*)

Ref Sequence

ENSEMBL: ENSMUSP00000039452 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000039178] [ENSMUST00000131919]

AlphaFold

Q80Z71

Predicted Effect

probably null
Transcript: ENSMUST00000039178
AA Change: K603*

SMART Domains

Protein: ENSMUSP00000039452
Gene: ENSMUSG00000026725
AA Change: K603*

Domain	Start	End	E-Value	Type
signal peptide	1	26	N/A	INTRINSIC
coiled coil region	100	132	N/A	INTRINSIC
EGF_like	170	198	3.5e1	SMART
EGF	201	229	2.29e1	SMART
EGF_like	232	260	2.86e1	SMART
FN3	262	341	1.81e-8	SMART
FN3	351	432	1.08e-6	SMART
FN3	443	521	1.19e-8	SMART
FN3	531	608	2.64e-10	SMART
FN3	619	696	1.6e-9	SMART
FN3	707	784	9.04e-9	SMART
FN3	795	872	7.34e-9	SMART
FN3	883	960	9.04e-9	SMART
FN3	971	1048	1.07e-10	SMART
FN3	1059	1136	7.57e-11	SMART
FN3	1147	1224	4.59e-10	SMART
FN3	1235	1312	1.95e-4	SMART
FBG	1327	1539	1.16e-114	SMART

Predicted Effect

probably null
Transcript: ENSMUST00000131919
AA Change: K603*

SMART Domains

Protein: ENSMUSP00000115685
Gene: ENSMUSG00000026725
AA Change: K603*

Domain	Start	End	E-Value	Type
signal peptide	1	26	N/A	INTRINSIC
coiled coil region	100	132	N/A	INTRINSIC
EGF_like	170	198	3.5e1	SMART
EGF	201	229	2.29e1	SMART
EGF_like	232	260	2.86e1	SMART
FN3	262	341	1.81e-8	SMART
FN3	351	432	1.08e-6	SMART
FN3	443	521	1.19e-8	SMART
FN3	531	608	2.64e-10	SMART
FN3	619	696	1.6e-9	SMART
FN3	707	784	9.04e-9	SMART
FN3	795	872	7.57e-11	SMART
FN3	883	960	4.59e-10	SMART
FN3	971	1048	1.95e-4	SMART
FBG	1063	1275	1.16e-114	SMART

Coding Region Coverage

1x: 100.0%
3x: 100.0%
10x: 99.8%
20x: 99.2%

Validation Efficiency

Allele List at MGI

All alleles(2) : Targeted, other(2)

Other mutations in this stock

Total: 65 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
2610021A01Rik	G	A	7: 41,626,976	G701D	probably damaging	Het
6030468B19Rik	T	A	11: 117,802,954	N82K	probably damaging	Het
Abca17	A	T	17: 24,335,590	V130E	possibly damaging	Het
Alpk2	T	A	18: 65,266,277	K2077*	probably null	Het
Aox2	T	C	1: 58,283,492	F73S	probably benign	Het
Ap3b1	T	A	13: 94,532,034	C965*	probably null	Het
Arhgef10	T	A	8: 14,930,030	V90E	probably damaging	Het
Atp5s	A	T	12: 69,741,788	N154Y	probably benign	Het
Atr	T	A	9: 95,869,900	H523Q	probably benign	Het
Bicdl1	G	T	5: 115,651,857	Q511K	possibly damaging	Het
Capn15	A	T	17: 25,965,254	N150K	probably damaging	Het
Card6	A	T	15: 5,100,109	W602R	probably benign	Het
Clnk	A	T	5: 38,769,891	S82T	possibly damaging	Het
Cmah	T	C	13: 24,436,629	I282T	probably damaging	Het
Cog8	A	G	8: 107,052,499	I382T	probably benign	Het
Dhrs7c	G	T	11: 67,809,896		probably null	Het
Dhrs9	T	A	2: 69,393,158	D83E	probably damaging	Het
Dmgdh	G	A	13: 93,715,535	G624E	probably damaging	Het
Dnajc30	C	A	5: 135,064,715	F155L	probably damaging	Het
Drc7	A	C	8: 95,074,150	K600T	probably damaging	Het
Efhb	A	C	17: 53,400,900	I745S	probably damaging	Het
Fasn	T	A	11: 120,810,465	K1988*	probably null	Het
Gad2	T	C	2: 22,635,023	L273P	probably damaging	Het
Gm45861	A	T	8: 27,542,508	K887*	probably null	Het
Gm45861	A	T	8: 27,542,509	K887I	unknown	Het
Gm9195	A	G	14: 72,480,781	Y152H	probably damaging	Het
Grip1	G	A	10: 119,945,156	D237N	probably damaging	Het
Hamp	A	C	7: 30,942,536	C65G	possibly damaging	Het
Hes3	A	G	4: 152,288,124		probably benign	Het
Hgh1	T	C	15: 76,370,450	I342T	probably damaging	Het
Hlcs	T	A	16: 94,268,164	K66*	probably null	Het
Ift80	A	T	3: 68,990,944	C19*	probably null	Het
Ikbkap	ACTTCTTCTTCTTCTTCTTCTTC	ACTTCTTCTTCTTCTTCTTC	4: 56,781,176		probably benign	Het
Kbtbd12	C	T	6: 88,618,668	C60Y	probably damaging	Het
Kdm4c	T	A	4: 74,345,567	V696E	probably benign	Het
Lect2	A	G	13: 56,542,990	I117T	unknown	Het
Lipn	T	A	19: 34,076,990	Y209*	probably null	Het
Lpin3	G	A	2: 160,898,707	V391I	probably benign	Het
Mocs2	A	T	13: 114,824,607	I47L	probably benign	Het
Mogs	T	A	6: 83,118,507	H768Q	probably benign	Het
Nbeal1	T	A	1: 60,237,158	I686N	probably damaging	Het
Nbeal1	T	A	1: 60,260,634	C1376*	probably null	Het
Ninl	A	G	2: 150,949,343	Y1087H	possibly damaging	Het
Olfr1076	T	A	2: 86,509,025	C189S	probably damaging	Het
Olfr1338	A	G	4: 118,754,418	V42A	possibly damaging	Het
Olfr518	A	G	7: 108,881,268	F113L	probably benign	Het
Olfr623	A	C	7: 103,660,581	L223R	probably damaging	Het
Olfr729	A	G	14: 50,148,232	L214S	probably damaging	Het
Olfr750	A	G	14: 51,071,159	V78A	possibly damaging	Het
Pkn1	A	G	8: 83,671,734	F768S	probably damaging	Het
Plekha2	A	G	8: 25,063,941	F82L	probably damaging	Het
Ppie	T	C	4: 123,135,107	E111G	probably benign	Het
Rps6ka5	T	C	12: 100,581,420	Y277C	probably damaging	Het
Scgb2b12	A	G	7: 32,326,677	V30A	probably benign	Het
Serac1	A	T	17: 6,074,201	S16T	probably benign	Het
Sf3a3	T	C	4: 124,722,900	Y192H	probably damaging	Het
Slc14a1	A	G	18: 78,102,411	V436A	probably benign	Het
Smc3	T	A	19: 53,641,898	M1112K	probably damaging	Het
Tnk2	G	A	16: 32,681,168	G1017E	probably damaging	Het
Trpm6	T	C	19: 18,838,098	S1183P	possibly damaging	Het
Ttn	A	T	2: 76,784,380	V16964E	possibly damaging	Het
Usp17le	G	A	7: 104,768,489	T482I	probably benign	Het
Vmn1r219	T	A	13: 23,163,355	M238K	probably damaging	Het
Wfs1	C	T	5: 36,967,172	G792S	probably benign	Het
Xpa	A	T	4: 46,185,612	L122Q	probably damaging	Het

Other mutations in Tnn

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00095:Tnn	APN	1	160125451	missense	possibly damaging	0.65
IGL00433:Tnn	APN	1	160098206	splice site	probably benign
IGL00858:Tnn	APN	1	160088392	critical splice donor site	probably null
IGL00939:Tnn	APN	1	160147530	missense	probably damaging	1.00
IGL01569:Tnn	APN	1	160120554	missense	possibly damaging	0.51
IGL01591:Tnn	APN	1	160125574	missense	probably damaging	1.00
IGL01628:Tnn	APN	1	160147602	missense	possibly damaging	0.89
IGL01811:Tnn	APN	1	160107135	missense	probably damaging	1.00
IGL01813:Tnn	APN	1	160088438	missense	probably damaging	1.00
IGL02340:Tnn	APN	1	160145205	missense	probably benign	0.00
IGL02488:Tnn	APN	1	160140593	missense	probably benign	0.21
IGL02535:Tnn	APN	1	160122652	splice site	probably null
IGL02563:Tnn	APN	1	160114553	missense	probably damaging	1.00
IGL02572:Tnn	APN	1	160086107	missense	probably damaging	1.00
IGL02740:Tnn	APN	1	160140777	splice site	probably benign
IGL02818:Tnn	APN	1	160116278	missense	possibly damaging	0.86
IGL03284:Tnn	APN	1	160125452	missense	probably benign	0.01
1mM(1):Tnn	UTSW	1	160097341	missense	probably damaging	1.00
PIT4305001:Tnn	UTSW	1	160086077	missense	possibly damaging	0.91
R0023:Tnn	UTSW	1	160104928	missense	probably benign	0.00
R0234:Tnn	UTSW	1	160088466	missense	probably damaging	1.00
R0234:Tnn	UTSW	1	160088466	missense	probably damaging	1.00
R0316:Tnn	UTSW	1	160120567	missense	possibly damaging	0.93
R0492:Tnn	UTSW	1	160120757	missense	probably damaging	0.99
R0547:Tnn	UTSW	1	160116337	intron	probably benign
R1067:Tnn	UTSW	1	160125398	missense	probably damaging	1.00
R1563:Tnn	UTSW	1	160125415	missense	probably damaging	1.00
R1565:Tnn	UTSW	1	160097265	missense	probably damaging	1.00
R1615:Tnn	UTSW	1	160118408	missense	possibly damaging	0.93
R1637:Tnn	UTSW	1	160147600	missense	probably damaging	1.00
R1707:Tnn	UTSW	1	160145144	missense	probably damaging	1.00
R1758:Tnn	UTSW	1	160147584	missense	possibly damaging	0.61
R1797:Tnn	UTSW	1	160140688	missense	probably damaging	1.00
R1847:Tnn	UTSW	1	160116182	missense	possibly damaging	0.51
R1925:Tnn	UTSW	1	160097229	missense	probably damaging	1.00
R2182:Tnn	UTSW	1	160140600	splice site	probably null
R2196:Tnn	UTSW	1	160097228	nonsense	probably null
R2225:Tnn	UTSW	1	160147465	missense	probably damaging	1.00
R2227:Tnn	UTSW	1	160147465	missense	probably damaging	1.00
R2286:Tnn	UTSW	1	160110509	missense	possibly damaging	0.89
R2850:Tnn	UTSW	1	160139287	missense	probably benign	0.00
R3110:Tnn	UTSW	1	160116286	missense	possibly damaging	0.71
R3111:Tnn	UTSW	1	160107055	missense	probably damaging	0.98
R3112:Tnn	UTSW	1	160116286	missense	possibly damaging	0.71
R3729:Tnn	UTSW	1	160146240	missense	probably damaging	1.00
R4183:Tnn	UTSW	1	160097355	missense	probably damaging	1.00
R4439:Tnn	UTSW	1	160116080	missense	probably benign
R4441:Tnn	UTSW	1	160116080	missense	probably benign
R4588:Tnn	UTSW	1	160145111	missense	probably benign	0.25
R4646:Tnn	UTSW	1	160146042	missense	probably benign
R4647:Tnn	UTSW	1	160146042	missense	probably benign
R4648:Tnn	UTSW	1	160146042	missense	probably benign
R4701:Tnn	UTSW	1	160147768	missense	possibly damaging	0.72
R4703:Tnn	UTSW	1	160116245	missense	possibly damaging	0.84
R4737:Tnn	UTSW	1	160146089	missense	probably damaging	1.00
R4801:Tnn	UTSW	1	160145033	missense	possibly damaging	0.90
R4802:Tnn	UTSW	1	160145033	missense	possibly damaging	0.90
R4868:Tnn	UTSW	1	160130873	missense	possibly damaging	0.64
R4977:Tnn	UTSW	1	160120618	missense	probably damaging	1.00
R5011:Tnn	UTSW	1	160126379	missense	possibly damaging	0.89
R5026:Tnn	UTSW	1	160146137	missense	probably benign	0.00
R5027:Tnn	UTSW	1	160145211	missense	probably damaging	1.00
R5049:Tnn	UTSW	1	160140738	missense	probably benign	0.00
R5119:Tnn	UTSW	1	160120552	missense	probably damaging	0.98
R5128:Tnn	UTSW	1	160122894	missense	probably damaging	0.98
R5234:Tnn	UTSW	1	160144999	missense	possibly damaging	0.95
R5398:Tnn	UTSW	1	160147522	missense	probably benign	0.00
R5424:Tnn	UTSW	1	160122702	missense	possibly damaging	0.69
R5452:Tnn	UTSW	1	160110261	missense	probably benign	0.13
R5466:Tnn	UTSW	1	160120536	missense	possibly damaging	0.93
R6022:Tnn	UTSW	1	160110358	missense	probably benign	0.00
R6062:Tnn	UTSW	1	160098278	missense	probably damaging	1.00
R6086:Tnn	UTSW	1	160086120	missense	probably damaging	1.00
R6132:Tnn	UTSW	1	160146071	missense	probably damaging	0.96
R6324:Tnn	UTSW	1	160145204	missense	probably damaging	0.96
R6455:Tnn	UTSW	1	160114719	missense	probably damaging	1.00
R6563:Tnn	UTSW	1	160088398	missense	probably damaging	1.00
R6650:Tnn	UTSW	1	160114583	missense	probably damaging	1.00
R6806:Tnn	UTSW	1	160120708	missense	possibly damaging	0.95
R6810:Tnn	UTSW	1	160104842	missense	probably damaging	1.00
R7243:Tnn	UTSW	1	160107117	missense	probably benign	0.07
R7340:Tnn	UTSW	1	160146022	missense	probably damaging	0.98
R7472:Tnn	UTSW	1	160110347	missense	probably benign	0.12
R7502:Tnn	UTSW	1	160110359	missense	probably benign	0.00
R7527:Tnn	UTSW	1	160118504	missense	possibly damaging	0.51
R7608:Tnn	UTSW	1	160088414	nonsense	probably null
R7746:Tnn	UTSW	1	160114685	missense	probably damaging	0.97
R8096:Tnn	UTSW	1	160122841	missense	probably damaging	1.00
R8136:Tnn	UTSW	1	160107060	missense	probably damaging	0.96
R8191:Tnn	UTSW	1	160125518	missense	probably damaging	1.00
R8334:Tnn	UTSW	1	160118483	missense	probably damaging	1.00
R8335:Tnn	UTSW	1	160118483	missense	probably damaging	1.00
R8337:Tnn	UTSW	1	160118483	missense	probably damaging	1.00
R8338:Tnn	UTSW	1	160118483	missense	probably damaging	1.00
R8427:Tnn	UTSW	1	160130686	missense	probably damaging	0.99
R8433:Tnn	UTSW	1	160097220	missense	possibly damaging	0.81
R8479:Tnn	UTSW	1	160122827	missense	probably benign	0.06
R8505:Tnn	UTSW	1	160146023	missense	probably damaging	0.98
R8554:Tnn	UTSW	1	160110416	missense	probably damaging	1.00
R8717:Tnn	UTSW	1	160116276	missense	possibly damaging	0.51
R8850:Tnn	UTSW	1	160110244	critical splice donor site	probably null
R8928:Tnn	UTSW	1	160125529	missense	probably damaging	1.00
R9209:Tnn	UTSW	1	160126416	missense	probably benign	0.02
X0019:Tnn	UTSW	1	160086146	missense	probably damaging	1.00
Z1176:Tnn	UTSW	1	160146293	missense	probably benign
Z1177:Tnn	UTSW	1	160126527	missense	probably damaging	0.98

Predicted Primers

PCR Primer
(F):5'- CTAAAAGCGTCACTCCTGTGC -3'
(R):5'- GTGACAGAGAACAGCCTCTC -3'

Sequencing Primer
(F):5'- ACTTAGATTGTGCCGCAAGC -3'
(R):5'- TCTCTGTCTCGTGGGACCCAG -3'

Posted On

2019-06-26