Incidental Mutation 'R7158:Alox8'
ID 557456
Institutional Source Beutler Lab
Gene Symbol Alox8
Ensembl Gene ENSMUSG00000020891
Gene Name arachidonate 8-lipoxygenase
Synonyms 8S-LOX, 8S-lipoxygenase, 8-LOX, Alox15b
MMRRC Submission 045329-MU
Accession Numbers
Essential gene? Probably non essential (E-score: 0.114) question?
Stock # R7158 (G1)
Quality Score 225.009
Status Validated
Chromosome 11
Chromosomal Location 69074758-69088669 bp(-) (GRCm39)
Type of Mutation missense
DNA Base Change (assembly) T to A at 69076696 bp (GRCm39)
Zygosity Heterozygous
Amino Acid Change Methionine to Leucine at position 568 (M568L)
Ref Sequence ENSEMBL: ENSMUSP00000021262 (fasta)
Gene Model predicted gene model for transcript(s): [ENSMUST00000021262] [ENSMUST00000094078]
AlphaFold O35936
Predicted Effect probably benign
Transcript: ENSMUST00000021262
AA Change: M568L

PolyPhen 2 Score 0.003 (Sensitivity: 0.98; Specificity: 0.44)
SMART Domains Protein: ENSMUSP00000021262
Gene: ENSMUSG00000020891
AA Change: M568L

DomainStartEndE-ValueType
LH2 2 122 1.07e-36 SMART
Pfam:Lipoxygenase 163 662 5.5e-70 PFAM
Predicted Effect probably benign
Transcript: ENSMUST00000094078
AA Change: M539L

PolyPhen 2 Score 0.046 (Sensitivity: 0.94; Specificity: 0.83)
SMART Domains Protein: ENSMUSP00000091621
Gene: ENSMUSG00000020891
AA Change: M539L

DomainStartEndE-ValueType
LH2 2 122 1.07e-36 SMART
Pfam:Lipoxygenase 205 405 6.9e-44 PFAM
Pfam:Lipoxygenase 402 640 1.9e-60 PFAM
Coding Region Coverage
  • 1x: 100.0%
  • 3x: 99.9%
  • 10x: 99.7%
  • 20x: 98.8%
Validation Efficiency 100% (68/68)
MGI Phenotype FUNCTION: This gene belongs to the lipoxygenase (LOX) gene family whose members encode enzymes that catalyze the addition of molecular oxygen to polyunsaturated fatty acids (PUFAs) to yield fatty acid hydroperoxides. The encoded enzyme preferentially metabolizes arachidonic acid to yield 8-hydroxyeicosatetraenoic acid (8-HETE), while metabolizing linoleic acid less efficiently. The gene may also function as a tumor suppressor. This gene is located in a cluster of related genes that spans approximately 75 kilobases on chromosome 11. [provided by RefSeq, Jan 2013]
Allele List at MGI
Other mutations in this stock
Total: 65 list
GeneRefVarChr/LocMutationPredicted EffectZygosity
Abca13 A T 11: 9,223,982 (GRCm39) M454L probably benign Het
Abca8b T A 11: 109,825,415 (GRCm39) E1595V probably damaging Het
Actr5 T A 2: 158,468,334 (GRCm39) C155S possibly damaging Het
Acvr1b T A 15: 101,091,939 (GRCm39) V73E probably benign Het
Add2 T C 6: 86,062,934 (GRCm39) Y31H probably damaging Het
Akap13 G T 7: 75,229,342 (GRCm39) V92F probably damaging Het
Birc6 A T 17: 74,901,371 (GRCm39) E1145V probably benign Het
Bltp3a T A 17: 28,105,407 (GRCm39) C644* probably null Het
Cacng1 A T 11: 107,594,665 (GRCm39) M166K probably damaging Het
Ces1f A G 8: 93,994,644 (GRCm39) F256L probably benign Het
Clpb A G 7: 101,313,039 (GRCm39) R8G probably benign Het
Col6a5 A G 9: 105,741,407 (GRCm39) V2504A possibly damaging Het
Coq8a T C 1: 180,006,749 (GRCm39) D93G probably benign Het
Cry2 A T 2: 92,244,060 (GRCm39) I371N probably damaging Het
Ddx43 A C 9: 78,319,501 (GRCm39) Q276H probably damaging Het
Dock10 T C 1: 80,564,589 (GRCm39) probably null Het
Dst A G 1: 34,313,366 (GRCm39) T4378A probably benign Het
Fezf1 T C 6: 23,245,789 (GRCm39) T459A probably benign Het
Gm10097 C T 10: 5,019,407 (GRCm39) A72V unknown Het
Hydin A G 8: 111,336,303 (GRCm39) S5027G possibly damaging Het
Inhbb A T 1: 119,348,752 (GRCm39) L22* probably null Het
Kat8 G A 7: 127,521,331 (GRCm39) G228S probably benign Het
Kif15 T A 9: 122,828,379 (GRCm39) S897T probably benign Het
Kndc1 A G 7: 139,511,773 (GRCm39) Y1460C possibly damaging Het
Krt78 T A 15: 101,860,241 (GRCm39) D225V probably benign Het
Lama3 A T 18: 12,589,869 (GRCm39) I800F probably benign Het
Mdn1 C A 4: 32,725,121 (GRCm39) T2580K probably benign Het
Mest T C 6: 30,744,913 (GRCm39) F201S possibly damaging Het
Mtmr9 A G 14: 63,764,318 (GRCm39) F470L probably benign Het
Nfic C T 10: 81,256,439 (GRCm39) R75Q probably damaging Het
Nme2 G A 11: 93,846,484 (GRCm39) probably benign Het
Nrg4 A G 9: 55,149,384 (GRCm39) L71P probably damaging Het
Pacsin2 C T 15: 83,263,943 (GRCm39) E365K possibly damaging Het
Pappa T C 4: 65,123,104 (GRCm39) I813T possibly damaging Het
Pcdhgb4 A G 18: 37,853,938 (GRCm39) E111G probably damaging Het
Pdzd8 C T 19: 59,288,589 (GRCm39) R937H probably damaging Het
Per1 T G 11: 68,994,930 (GRCm39) probably benign Het
Pex2 A G 3: 5,626,396 (GRCm39) F138L probably benign Het
Pold1 A T 7: 44,188,290 (GRCm39) N529K probably damaging Het
Pou6f2 T C 13: 18,326,623 (GRCm39) I316V Het
Prom1 A C 5: 44,170,255 (GRCm39) I682S probably damaging Het
Prpf40a T C 2: 53,042,565 (GRCm39) K481E probably damaging Het
Prrg4 A T 2: 104,662,958 (GRCm39) V216E probably damaging Het
Ptch2 C T 4: 116,971,981 (GRCm39) P1168S possibly damaging Het
Pvr C A 7: 19,652,562 (GRCm39) E118* probably null Het
R3hcc1l C T 19: 42,571,868 (GRCm39) P716S probably damaging Het
Rasa4 A G 5: 136,130,875 (GRCm39) E382G probably damaging Het
Rbp3 T A 14: 33,677,513 (GRCm39) M487K probably benign Het
Rsad2 A T 12: 26,500,779 (GRCm39) probably null Het
Shmt1 T C 11: 60,681,068 (GRCm39) I353V probably benign Het
Shprh C T 10: 11,042,474 (GRCm39) T819I probably damaging Het
Skap1 T A 11: 96,416,883 (GRCm39) F56Y possibly damaging Het
Slc22a16 G T 10: 40,449,737 (GRCm39) V79L possibly damaging Het
Slc34a1 A G 13: 55,549,044 (GRCm39) T165A probably damaging Het
Smchd1 A C 17: 71,707,145 (GRCm39) I941R probably damaging Het
Syne1 C A 10: 5,007,931 (GRCm39) V98F probably damaging Het
Tcaim A G 9: 122,648,055 (GRCm39) D190G possibly damaging Het
Tdrd9 G C 12: 112,002,800 (GRCm39) E816D probably benign Het
Tmem132d T C 5: 128,214,083 (GRCm39) K326E possibly damaging Het
Usp35 T C 7: 96,975,171 (GRCm39) M1V probably null Het
Wdr62 C T 7: 29,970,163 (GRCm39) V215I possibly damaging Het
Zan T C 5: 137,398,906 (GRCm39) T4153A unknown Het
Zfp239 G T 6: 117,848,690 (GRCm39) E143* probably null Het
Zfp292 T C 4: 34,808,679 (GRCm39) D1460G probably benign Het
Zfp647 C T 15: 76,801,505 (GRCm39) G90R probably benign Het
Other mutations in Alox8
AlleleSourceChrCoordTypePredicted EffectPPH Score
IGL01411:Alox8 APN 11 69,079,516 (GRCm39) missense probably benign
IGL01878:Alox8 APN 11 69,087,864 (GRCm39) missense probably benign 0.00
IGL02342:Alox8 APN 11 69,077,053 (GRCm39) missense probably damaging 1.00
IGL02694:Alox8 APN 11 69,077,455 (GRCm39) missense probably damaging 0.99
IGL03246:Alox8 APN 11 69,076,841 (GRCm39) missense probably damaging 1.00
IGL03373:Alox8 APN 11 69,077,443 (GRCm39) missense probably benign 0.00
R0567:Alox8 UTSW 11 69,082,348 (GRCm39) critical splice donor site probably null
R1575:Alox8 UTSW 11 69,076,067 (GRCm39) missense possibly damaging 0.94
R1688:Alox8 UTSW 11 69,080,732 (GRCm39) missense probably benign 0.01
R2021:Alox8 UTSW 11 69,077,114 (GRCm39) missense probably damaging 0.98
R2041:Alox8 UTSW 11 69,088,517 (GRCm39) missense possibly damaging 0.67
R2175:Alox8 UTSW 11 69,078,592 (GRCm39) missense possibly damaging 0.85
R2237:Alox8 UTSW 11 69,076,597 (GRCm39) missense probably benign 0.00
R3821:Alox8 UTSW 11 69,077,308 (GRCm39) missense probably damaging 0.98
R4870:Alox8 UTSW 11 69,077,394 (GRCm39) missense probably damaging 1.00
R6836:Alox8 UTSW 11 69,080,715 (GRCm39) missense possibly damaging 0.82
R6836:Alox8 UTSW 11 69,077,331 (GRCm39) missense probably damaging 1.00
R7003:Alox8 UTSW 11 69,082,416 (GRCm39) missense possibly damaging 0.70
R7316:Alox8 UTSW 11 69,077,064 (GRCm39) missense probably benign 0.01
R7513:Alox8 UTSW 11 69,078,670 (GRCm39) missense probably benign 0.34
R9515:Alox8 UTSW 11 69,075,950 (GRCm39) missense probably damaging 1.00
R9721:Alox8 UTSW 11 69,087,911 (GRCm39) missense probably benign 0.19
X0065:Alox8 UTSW 11 69,076,079 (GRCm39) missense probably damaging 1.00
Z1177:Alox8 UTSW 11 69,076,047 (GRCm39) missense probably damaging 1.00
Z1186:Alox8 UTSW 11 69,088,322 (GRCm39) missense probably benign
Z1186:Alox8 UTSW 11 69,076,873 (GRCm39) missense probably benign 0.00
Z1187:Alox8 UTSW 11 69,088,322 (GRCm39) missense probably benign
Z1187:Alox8 UTSW 11 69,076,873 (GRCm39) missense probably benign 0.00
Z1188:Alox8 UTSW 11 69,088,322 (GRCm39) missense probably benign
Z1188:Alox8 UTSW 11 69,076,873 (GRCm39) missense probably benign 0.00
Z1189:Alox8 UTSW 11 69,088,322 (GRCm39) missense probably benign
Z1189:Alox8 UTSW 11 69,076,873 (GRCm39) missense probably benign 0.00
Z1190:Alox8 UTSW 11 69,088,322 (GRCm39) missense probably benign
Z1190:Alox8 UTSW 11 69,076,873 (GRCm39) missense probably benign 0.00
Z1191:Alox8 UTSW 11 69,088,322 (GRCm39) missense probably benign
Z1191:Alox8 UTSW 11 69,076,873 (GRCm39) missense probably benign 0.00
Z1192:Alox8 UTSW 11 69,088,322 (GRCm39) missense probably benign
Z1192:Alox8 UTSW 11 69,076,873 (GRCm39) missense probably benign 0.00
Predicted Primers PCR Primer
(F):5'- ACAATAGATCCGTTCCTATCCAG -3'
(R):5'- TTGTTGGATACCCGGGAAGC -3'

Sequencing Primer
(F):5'- AATAGATCCGTTCCTATCCAGCCTAG -3'
(R):5'- GAAGCCCTGGTCCAGTATATC -3'
Posted On 2019-06-26