Mutagenetix > Incidental Mutation

Incidental Mutation 'R7164:Gpr89'

557782

Institutional Source

Beutler Lab

Gene Symbol

Gpr89

Ensembl Gene

ENSMUSG00000028096

Gene Name

G protein-coupled receptor 89

Synonyms

SH120, 4933412D19Rik

MMRRC Submission

045331-MU

Accession Numbers

Essential gene?

Possibly non essential (E-score: 0.445) question?

Stock #

R7164 (G1)

Quality Score

225.009

Status

Validated

Chromosome

Chromosomal Location

96775630-96812662 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

A to T at 96778714 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Methionine to Lysine at position 453 (M453K)

Ref Sequence

ENSEMBL: ENSMUSP00000029738 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000029738] [ENSMUST00000058865] [ENSMUST00000107069] [ENSMUST00000107070] [ENSMUST00000153256]

AlphaFold

Q8BS95

Predicted Effect

probably benign
Transcript: ENSMUST00000029738
AA Change: M453K

PolyPhen 2 Score 0.041 (Sensitivity: 0.94; Specificity: 0.83)

SMART Domains

Protein: ENSMUSP00000029738
Gene: ENSMUSG00000028096
AA Change: M453K

Domain	Start	End	E-Value	Type
transmembrane domain	4	26	N/A	INTRINSIC
transmembrane domain	38	60	N/A	INTRINSIC
transmembrane domain	75	97	N/A	INTRINSIC
transmembrane domain	110	127	N/A	INTRINSIC
Pfam:GPHR_N	140	207	1.1e-31	PFAM
Pfam:ABA_GPCR	276	446	4.9e-46	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000058865

SMART Domains

Protein: ENSMUSP00000058936
Gene: ENSMUSG00000038298

Domain	Start	End	E-Value	Type
PDZ	18	90	2.41e-17	SMART
PDZ	143	215	1e-14	SMART
PDZ	251	323	2.81e-18	SMART
PDZ	386	458	4.5e-17	SMART
low complexity region	505	514	N/A	INTRINSIC

Predicted Effect

probably benign
Transcript: ENSMUST00000107069

SMART Domains

Protein: ENSMUSP00000102684
Gene: ENSMUSG00000038298

Domain	Start	End	E-Value	Type
PDZ	18	90	2.41e-17	SMART
PDZ	143	215	1e-14	SMART
PDZ	251	323	2.81e-18	SMART
PDZ	386	458	4.5e-17	SMART
low complexity region	505	514	N/A	INTRINSIC

Predicted Effect

probably benign
Transcript: ENSMUST00000107070

SMART Domains

Protein: ENSMUSP00000102685
Gene: ENSMUSG00000038298

Domain	Start	End	E-Value	Type
PDZ	18	90	2.41e-17	SMART
PDZ	143	215	1e-14	SMART
PDZ	251	323	2.81e-18	SMART
PDZ	386	458	4.5e-17	SMART
low complexity region	505	514	N/A	INTRINSIC

Predicted Effect

probably benign
Transcript: ENSMUST00000153256

SMART Domains

Protein: ENSMUSP00000118846
Gene: ENSMUSG00000038298

Domain	Start	End	E-Value	Type
PDZ	18	90	2.41e-17	SMART
PDZ	143	215	1e-14	SMART
PDZ	251	323	2.81e-18	SMART
PDZ	386	458	4.5e-17	SMART
low complexity region	505	514	N/A	INTRINSIC

Coding Region Coverage

1x: 100.0%
3x: 100.0%
10x: 99.7%
20x: 99.2%

Validation Efficiency

100% (59/59)

Allele List at MGI

Other mutations in this stock

Total: 60 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Actn2	G	A	13: 12,293,847 (GRCm39)	H558Y	probably damaging	Het
Ajm1	T	A	2: 25,468,579 (GRCm39)	H444L	possibly damaging	Het
Akap9	G	C	5: 4,110,364 (GRCm39)	E3022D	probably damaging	Het
Anapc2	C	T	2: 25,175,011 (GRCm39)	R710C	probably damaging	Het
Bcl6	G	A	16: 23,784,976 (GRCm39)	R675*	probably null	Het
Carmil3	A	G	14: 55,738,739 (GRCm39)	E844G	probably damaging	Het
Cdk17	T	G	10: 93,068,343 (GRCm39)	S367A	probably benign	Het
Cfap206	T	A	4: 34,719,656 (GRCm39)	M253L	probably benign	Het
Chd3	T	G	11: 69,253,132 (GRCm39)	K228Q	probably damaging	Het
Cit	T	A	5: 116,123,846 (GRCm39)	I1503N	possibly damaging	Het
Csn1s1	A	G	5: 87,822,087 (GRCm39)	N119S	possibly damaging	Het
Czib	C	A	4: 107,752,087 (GRCm39)	D155E	not run	Het
Degs1	A	G	1: 182,106,690 (GRCm39)	S226P	probably damaging	Het
Espl1	T	C	15: 102,221,638 (GRCm39)	W976R	probably damaging	Het
Fbxl4	C	T	4: 22,386,218 (GRCm39)	P275L	probably benign	Het
Flnb	A	G	14: 7,915,944 (GRCm38)		probably null	Het
Gnptab	T	A	10: 88,269,932 (GRCm39)	Y878*	probably null	Het
Igsf5	A	T	16: 96,174,048 (GRCm39)	Q26L	possibly damaging	Het
Inpp5e	T	A	2: 26,297,995 (GRCm39)	D202V	possibly damaging	Het
Itga3	C	T	11: 94,943,305 (GRCm39)	V931M	possibly damaging	Het
Kcnab3	T	C	11: 69,222,184 (GRCm39)		probably null	Het
Klk4	T	C	7: 43,531,122 (GRCm39)	I17T	possibly damaging	Het
Lrrc73	T	C	17: 46,567,169 (GRCm39)	L206P	probably damaging	Het
Manba	A	T	3: 135,248,149 (GRCm39)	N346I	probably damaging	Het
Map4k4	T	C	1: 40,013,132 (GRCm39)	Y76H	possibly damaging	Het
Map4k5	T	C	12: 69,877,210 (GRCm39)	T312A	probably benign	Het
Masp2	A	G	4: 148,694,572 (GRCm39)		probably null	Het
Mast1	T	C	8: 85,661,933 (GRCm39)	D63G	possibly damaging	Het
Mtf2	T	A	5: 108,241,235 (GRCm39)	S254T	possibly damaging	Het
Myo16	A	T	8: 10,619,585 (GRCm39)	T1379S	unknown	Het
Myo5a	A	G	9: 75,087,435 (GRCm39)	E1097G	probably benign	Het
Nat1	T	C	8: 67,944,329 (GRCm39)	V238A	possibly damaging	Het
Nhlrc2	A	G	19: 56,580,931 (GRCm39)	D493G	probably damaging	Het
Or10ak9	C	T	4: 118,726,922 (GRCm39)	P315S	probably benign	Het
Or1j21	T	C	2: 36,683,709 (GRCm39)	S154P	probably benign	Het
Or5ae2	A	G	7: 84,506,251 (GRCm39)	I227V	possibly damaging	Het
Or5b108	A	T	19: 13,168,270 (GRCm39)	M80L	probably benign	Het
Or5d20-ps1	T	C	2: 87,932,176 (GRCm39)	K52E	probably damaging	Het
Or8g51	A	G	9: 38,609,515 (GRCm39)	I49T	possibly damaging	Het
Pcsk5	C	T	19: 17,429,349 (GRCm39)	C1543Y	probably damaging	Het
Pde4d	A	G	13: 109,169,222 (GRCm39)	D88G	probably benign	Het
Pld5	A	T	1: 176,041,187 (GRCm39)	M1K	probably null	Het
Prmt6	A	G	3: 110,157,680 (GRCm39)	M203T	probably benign	Het
Prr14l	A	T	5: 32,986,510 (GRCm39)	V995D	probably damaging	Het
Psg18	T	C	7: 18,084,862 (GRCm39)	E199G	possibly damaging	Het
Pth1r	A	G	9: 110,552,815 (GRCm39)	I439T	possibly damaging	Het
Ptprc	T	A	1: 138,045,600 (GRCm39)	I87F	probably benign	Het
Slc44a1	T	C	4: 53,528,711 (GRCm39)	S154P	probably benign	Het
Slco1c1	T	A	6: 141,487,855 (GRCm39)	Y192*	probably null	Het
Spag16	A	G	1: 70,764,025 (GRCm39)	H615R	possibly damaging	Het
Spata31h1	G	A	10: 82,122,063 (GRCm39)	T3649I	probably damaging	Het
Tas2r114	G	A	6: 131,666,728 (GRCm39)	A100V	possibly damaging	Het
U2af1l4	T	C	7: 30,264,544 (GRCm39)	S103P	probably benign	Het
Usp10	T	C	8: 120,668,847 (GRCm39)	S383P	probably damaging	Het
Vmn2r113	A	G	17: 23,167,137 (GRCm39)	R505G	probably benign	Het
Vmn2r75	A	C	7: 85,814,592 (GRCm39)	D300E	probably damaging	Het
Zfp318	T	A	17: 46,708,232 (GRCm39)		probably null	Het
Zfp318	T	C	17: 46,716,865 (GRCm39)	V999A	probably damaging	Het
Zfp324	A	T	7: 12,702,810 (GRCm39)	H58L	probably damaging	Het
Zfp707	A	G	15: 75,846,967 (GRCm39)	E339G	possibly damaging	Het

Other mutations in Gpr89

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00421:Gpr89	APN	3	96,805,839 (GRCm39)	missense	probably damaging	1.00
IGL00757:Gpr89	APN	3	96,778,850 (GRCm39)	missense	probably benign	0.00
IGL01114:Gpr89	APN	3	96,800,865 (GRCm39)	missense	probably damaging	0.98
IGL02417:Gpr89	APN	3	96,804,741 (GRCm39)	nonsense	probably null
explorer	UTSW	3	96,787,385 (GRCm39)	splice site	probably null
R0207:Gpr89	UTSW	3	96,778,796 (GRCm39)	missense	probably damaging	0.99
R0650:Gpr89	UTSW	3	96,804,640 (GRCm39)	splice site	probably benign
R0704:Gpr89	UTSW	3	96,787,484 (GRCm39)	critical splice acceptor site	probably null
R1496:Gpr89	UTSW	3	96,812,526 (GRCm39)	missense	probably benign	0.00
R1869:Gpr89	UTSW	3	96,782,975 (GRCm39)	missense	probably benign	0.16
R1913:Gpr89	UTSW	3	96,782,949 (GRCm39)	missense	possibly damaging	0.91
R2264:Gpr89	UTSW	3	96,779,831 (GRCm39)	missense	probably damaging	0.99
R2276:Gpr89	UTSW	3	96,804,743 (GRCm39)	missense	probably damaging	1.00
R3822:Gpr89	UTSW	3	96,800,260 (GRCm39)	missense	probably benign	0.03
R3922:Gpr89	UTSW	3	96,798,215 (GRCm39)	missense	probably damaging	1.00
R4984:Gpr89	UTSW	3	96,812,512 (GRCm39)	missense	probably benign	0.02
R5761:Gpr89	UTSW	3	96,800,196 (GRCm39)	missense	probably damaging	1.00
R6185:Gpr89	UTSW	3	96,798,149 (GRCm39)	missense	probably damaging	0.99
R7063:Gpr89	UTSW	3	96,783,014 (GRCm39)	missense	probably damaging	1.00
R7172:Gpr89	UTSW	3	96,787,385 (GRCm39)	splice site	probably null
R7215:Gpr89	UTSW	3	96,787,404 (GRCm39)	missense	probably damaging	1.00
R7536:Gpr89	UTSW	3	96,798,209 (GRCm39)	missense	probably damaging	0.96
R7708:Gpr89	UTSW	3	96,787,941 (GRCm39)	missense	possibly damaging	0.81
R7849:Gpr89	UTSW	3	96,778,806 (GRCm39)	nonsense	probably null
R8273:Gpr89	UTSW	3	96,812,505 (GRCm39)	missense	probably benign
R9643:Gpr89	UTSW	3	96,780,813 (GRCm39)	missense	probably damaging	0.97
RF019:Gpr89	UTSW	3	96,812,509 (GRCm39)	missense	probably benign

Predicted Primers

PCR Primer
(F):5'- TCACTGCACAGCCTCAGATC -3'
(R):5'- CAGGGTATGTACTTCGTGTCCTC -3'

Sequencing Primer
(F):5'- ACAGCCTCAGATCCATGCGG -3'
(R):5'- CTGTACTGCTGATCCGAATGAGC -3'

Posted On

2019-06-26