Mutagenetix > Incidental Mutations

Incidental Mutation 'R7166:Zfp960'

557960

Institutional Source

Beutler Lab

Gene Symbol

Zfp960

Ensembl Gene

ENSMUSG00000096696

Gene Name

zinc finger protein 960

Synonyms

BC018101

MMRRC Submission

Accession Numbers

Is this an essential gene?

Possibly non essential (E-score: 0.290) question?

Stock #

R7166 (G1)

Quality Score

225.009

Status

Not validated

Chromosome

Chromosomal Location

17064113-17089628 bp(+) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

T to A at 17088499 bp

Zygosity

Heterozygous

Amino Acid Change

Cysteine to Serine at position 492 (C492S)

Ref Sequence

ENSEMBL: ENSMUSP00000123102 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000127027]

Predicted Effect

probably damaging
Transcript: ENSMUST00000127027
AA Change: C492S

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)

SMART Domains

Protein: ENSMUSP00000123102
Gene: ENSMUSG00000096696
AA Change: C492S

Domain	Start	End	E-Value	Type
KRAB	13	82	4.42e-16	SMART
ZnF_C2H2	158	180	3.95e-4	SMART
ZnF_C2H2	186	208	4.4e-2	SMART
ZnF_C2H2	238	260	2.95e-3	SMART
ZnF_C2H2	266	288	6.32e-3	SMART
ZnF_C2H2	294	316	8.94e-3	SMART
ZnF_C2H2	322	344	2.53e-2	SMART
ZnF_C2H2	350	372	3.21e-4	SMART
ZnF_C2H2	378	400	3.89e-3	SMART
ZnF_C2H2	406	428	2.2e-2	SMART
ZnF_C2H2	434	456	7.9e-4	SMART
ZnF_C2H2	462	484	7.9e-4	SMART
ZnF_C2H2	490	512	7.78e-3	SMART
ZnF_C2H2	518	540	3.95e-4	SMART

Coding Region Coverage

1x: 100.0%
3x: 100.0%
10x: 99.7%
20x: 99.0%

Validation Efficiency

98% (52/53)

Allele List at MGI

Other mutations in this stock

Total: 53 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Ambn	T	C	5: 88,467,528	L272P	possibly damaging	Het
Ash2l	C	A	8: 25,827,320	G335V	probably damaging	Het
Atp13a1	T	A	8: 69,799,316		probably null	Het
Atp13a2	G	A	4: 141,006,984	R1139Q	possibly damaging	Het
Atxn2	T	A	5: 121,796,397	N886K	possibly damaging	Het
Celsr3	G	T	9: 108,842,951	C2512F	probably damaging	Het
Cfap221	A	G	1: 119,948,113	V449A	probably benign	Het
Cfhr2	A	T	1: 139,831,101	C70*	probably null	Het
Chfr	C	A	5: 110,158,805	P472Q	probably benign	Het
Crybg2	G	A	4: 134,060,882	R22Q	probably damaging	Het
Eef2k	T	C	7: 120,884,772	F244L	probably damaging	Het
Efcab11	A	T	12: 99,883,355	M23K		Het
Ercc8	T	A	13: 108,169,433	M114K	possibly damaging	Het
Fam217a	T	C	13: 34,910,315	Y487C	probably benign	Het
Farsb	T	C	1: 78,471,184	N205S	probably benign	Het
Glra1	A	G	11: 55,515,078	F370S	probably benign	Het
Gm12258	T	A	11: 58,858,473	M158K		Het
Gm14305	T	A	2: 176,720,943	H209Q	probably damaging	Het
Gm4924	A	T	10: 82,378,201	Q611L	unknown	Het
Gm5580	A	G	6: 116,551,368	I69V	probably benign	Het
Haus6	T	C	4: 86,583,687	E649G	possibly damaging	Het
Hist1h4j	A	G	13: 21,735,151	H19R	unknown	Het
Hlcs	C	T	16: 94,262,726	D345N	possibly damaging	Het
Htt	C	A	5: 34,852,894	Q1564K	probably benign	Het
Itpr1	G	A	6: 108,378,190	V481I	probably benign	Het
Jak3	T	C	8: 71,682,316	I531T	probably damaging	Het
Kng1	T	A	16: 23,079,678	H609Q	probably benign	Het
Mdn1	T	A	4: 32,746,446	S4131T	probably damaging	Het
Npnt	A	G	3: 132,948,128	S31P	probably damaging	Het
Olfr1098	T	A	2: 86,922,748	K261N	probably damaging	Het
Olfr1186	A	T	2: 88,525,646	Q21L	possibly damaging	Het
Olfr398	A	T	11: 73,984,295	F104L	possibly damaging	Het
Paxx	A	T	2: 25,460,226	L123Q	probably damaging	Het
Prdm13	C	T	4: 21,683,528	R144Q	unknown	Het
Rab2b	C	A	14: 52,279,345		probably benign	Het
Rnf207	A	G	4: 152,311,780	I509T	probably damaging	Het
Ropn1l	T	C	15: 31,453,509	Q12R		Het
Ryr3	T	G	2: 112,875,028	Y847S	probably damaging	Het
Slc1a6	A	T	10: 78,812,812	T456S	possibly damaging	Het
Slc26a2	A	T	18: 61,198,829	M510K	possibly damaging	Het
Slc5a9	T	C	4: 111,883,839	T537A	probably benign	Het
Slc9b2	T	C	3: 135,326,178	Y132H	unknown	Het
Sltm	T	C	9: 70,584,850	L725S	probably damaging	Het
Spz1	A	G	13: 92,575,927	C14R	probably benign	Het
Srrm4	T	A	5: 116,471,242	Q172L	unknown	Het
Synj2bp	T	C	12: 81,504,515	D92G	probably benign	Het
Tmem169	A	C	1: 72,301,070	T220P	probably benign	Het
Ttn	T	A	2: 76,888,028	I7270F	unknown	Het
Txndc16	T	G	14: 45,183,154	N137H	probably benign	Het
Ubr5	A	G	15: 37,976,145	Y2499H		Het
Ugt2b38	T	C	5: 87,410,446	D452G	probably damaging	Het
Zfp12	T	A	5: 143,245,502	I560N	possibly damaging	Het
Zfp60	A	G	7: 27,749,512	K535R	possibly damaging	Het

Other mutations in Zfp960

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
R1026:Zfp960	UTSW	17	17088256	missense	probably damaging	1.00
R1511:Zfp960	UTSW	17	17088256	missense	probably damaging	1.00
R2141:Zfp960	UTSW	17	17087884	missense	probably benign	0.09
R3729:Zfp960	UTSW	17	17088371	missense	probably damaging	1.00
R3730:Zfp960	UTSW	17	17088371	missense	probably damaging	1.00
R3731:Zfp960	UTSW	17	17088371	missense	probably damaging	1.00
R4799:Zfp960	UTSW	17	17088436	missense	probably damaging	1.00
R5418:Zfp960	UTSW	17	17087543	missense	probably damaging	1.00
R5513:Zfp960	UTSW	17	17087734	missense	possibly damaging	0.64
R6309:Zfp960	UTSW	17	17088377	missense	probably benign	0.08
R6836:Zfp960	UTSW	17	17088172	missense	probably damaging	1.00
R7528:Zfp960	UTSW	17	17087563	missense	possibly damaging	0.84
R7576:Zfp960	UTSW	17	17087965	missense	probably benign	0.05
R8063:Zfp960	UTSW	17	17088361	missense	probably benign	0.03
R8263:Zfp960	UTSW	17	17087940	nonsense	probably null
R8354:Zfp960	UTSW	17	17088199	missense	probably benign	0.00
R8454:Zfp960	UTSW	17	17088199	missense	probably benign	0.00

Predicted Primers

PCR Primer
(F):5'- CGTCACAGCACTCTTCAAAGACAT -3'
(R):5'- GTACTGTGACCTGCAAAGGCTT -3'

Sequencing Primer
(F):5'- AGCCTTTGCATGTCACAGATAC -3'
(R):5'- GCCACATTGATTACATTCATACGGG -3'

Posted On

2019-06-26