Mutagenetix > Incidental Mutation

Incidental Mutation 'R7175:Fam91a1'

558572

Institutional Source

Beutler Lab

Gene Symbol

Fam91a1

Ensembl Gene

ENSMUSG00000037119

Gene Name

family with sequence similarity 91, member A1

Synonyms

D15Ertd621e

MMRRC Submission

045231-MU

Accession Numbers

Essential gene?

Non essential (E-score: 0.000) question?

Stock #

R7175 (G1)

Quality Score

225.009

Status

Validated

Chromosome

Chromosomal Location

58287317-58329589 bp(+) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

A to T at 58302527 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Tyrosine to Phenylalanine at position 289 (Y289F)

Ref Sequence

ENSEMBL: ENSMUSP00000036524 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000037270]

AlphaFold

Q3UVG3

Predicted Effect

probably benign
Transcript: ENSMUST00000037270
AA Change: Y289F

PolyPhen 2 Score 0.061 (Sensitivity: 0.94; Specificity: 0.84)

SMART Domains

Protein: ENSMUSP00000036524
Gene: ENSMUSG00000037119
AA Change: Y289F

Domain	Start	End	E-Value	Type
Pfam:FAM91_N	8	312	2.8e-149	PFAM
Pfam:FAM91_C	374	821	3.8e-182	PFAM

Meta Mutation Damage Score

0.0638

Coding Region Coverage

1x: 100.0%
3x: 100.0%
10x: 99.7%
20x: 99.1%

Validation Efficiency

100% (63/63)

MGI Phenotype

PHENOTYPE: Mice homozygous for a transgenic gene disruption exhibit male infertility. [provided by MGI curators]

Allele List at MGI

Other mutations in this stock

Total: 63 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Abcb5	G	A	12: 118,831,611 (GRCm39)	T1247I	probably benign	Het
Afdn	T	C	17: 14,108,869 (GRCm39)	L1479P	probably damaging	Het
Alox12e	T	C	11: 70,210,534 (GRCm39)	R363G	probably damaging	Het
Ank2	A	T	3: 126,740,590 (GRCm39)	S1765T	unknown	Het
Anks6	T	C	4: 47,046,268 (GRCm39)		probably null	Het
Apob	A	C	12: 8,057,034 (GRCm39)	I1839L	probably benign	Het
Bdp1	C	A	13: 100,186,478 (GRCm39)	R1658I	probably damaging	Het
Ccs	T	A	19: 4,883,390 (GRCm39)	D136V	probably damaging	Het
Cd200	T	C	16: 45,220,578 (GRCm39)		probably null	Het
Cirbp	T	C	10: 80,006,297 (GRCm39)	S130P	probably benign	Het
Cpb1	A	G	3: 20,317,927 (GRCm39)	I199T	probably benign	Het
Csn3	C	T	5: 88,077,586 (GRCm39)	R31C	probably damaging	Het
Dcaf1	A	T	9: 106,735,775 (GRCm39)	I908F	probably benign	Het
Dhcr7	C	T	7: 143,399,227 (GRCm39)	T199I	probably damaging	Het
Dnah9	T	A	11: 66,024,463 (GRCm39)	Q277L	probably benign	Het
Echdc2	C	A	4: 108,031,366 (GRCm39)	P237T	probably damaging	Het
Efcab3	T	C	11: 104,838,237 (GRCm39)	V3625A	unknown	Het
Eif4g3	A	G	4: 137,853,526 (GRCm39)	N364S	probably damaging	Het
Eml6	T	A	11: 29,734,231 (GRCm39)	I1170L	probably benign	Het
Epha3	C	T	16: 63,403,863 (GRCm39)	R746Q	probably damaging	Het
Exosc5	G	A	7: 25,363,794 (GRCm39)	C102Y	probably damaging	Het
Fbxo38	A	C	18: 62,648,544 (GRCm39)	F665V	probably benign	Het
Fcrl5	T	C	3: 87,353,645 (GRCm39)	V330A	probably benign	Het
Fer	T	A	17: 64,231,090 (GRCm39)	D280E	probably benign	Het
Gpr158	A	G	2: 21,373,113 (GRCm39)	H16R	probably benign	Het
Gzmg	C	T	14: 56,396,979 (GRCm39)	M1I	probably null	Het
Hectd4	C	T	5: 121,411,692 (GRCm39)	A456V	possibly damaging	Het
Hk2	T	A	6: 82,711,830 (GRCm39)	Q613L	probably benign	Het
Inhca	A	G	9: 103,128,988 (GRCm39)		probably null	Het
Itsn1	T	C	16: 91,664,938 (GRCm39)	F1121L	unknown	Het
Lztr1	G	T	16: 17,340,895 (GRCm39)	C557F	possibly damaging	Het
Mdn1	A	G	4: 32,694,634 (GRCm39)	Y1119C	probably damaging	Het
Nfkb1	A	T	3: 135,319,751 (GRCm39)	L248Q	probably damaging	Het
Or1a1	T	A	11: 74,087,004 (GRCm39)	L225*	probably null	Het
Or1p1b	T	C	11: 74,130,803 (GRCm39)	F138L	probably benign	Het
Or2g7	T	A	17: 38,378,370 (GRCm39)	S103T	probably damaging	Het
Or52e19	T	C	7: 102,959,054 (GRCm39)	V42A	probably benign	Het
Otulinl	A	C	15: 27,658,374 (GRCm39)	D165E	probably damaging	Het
Pate1	T	G	9: 35,596,408 (GRCm39)	D119A	probably damaging	Het
Pcdhgc4	T	C	18: 37,949,424 (GRCm39)	V280A	possibly damaging	Het
Pik3ap1	T	C	19: 41,275,929 (GRCm39)	D717G	probably damaging	Het
Prdm13	A	T	4: 21,679,473 (GRCm39)	L339Q	unknown	Het
Rasgrf1	T	A	9: 89,862,802 (GRCm39)	N519K	probably benign	Het
Rergl	A	G	6: 139,473,533 (GRCm39)	V39A	probably benign	Het
Runx2	G	A	17: 45,125,079 (GRCm39)	P80L	probably damaging	Het
Sema4b	T	A	7: 79,848,402 (GRCm39)	M1K	probably null	Het
Slc20a1	T	G	2: 129,052,662 (GRCm39)	L648R	probably damaging	Het
Spata31h1	T	A	10: 82,122,583 (GRCm39)	S3476C	probably damaging	Het
Speg	A	G	1: 75,399,134 (GRCm39)	T2194A	probably benign	Het
Spns1	T	C	7: 125,972,961 (GRCm39)	D215G	probably damaging	Het
Tle4	A	T	19: 14,429,071 (GRCm39)	V717E	probably damaging	Het
Trim50	T	A	5: 135,382,151 (GRCm39)	M1K	probably null	Het
Trpa1	A	G	1: 14,963,431 (GRCm39)	V597A	possibly damaging	Het
Usp13	C	T	3: 32,971,757 (GRCm39)	Q746*	probably null	Het
Vmn1r35	A	T	6: 66,655,906 (GRCm39)	W255R	probably benign	Het
Vps35	A	T	8: 85,990,189 (GRCm39)		probably null	Het
Vps54	T	C	11: 21,265,028 (GRCm39)		probably null	Het
Zfp318	T	A	17: 46,697,774 (GRCm39)	L210Q	probably damaging	Het
Zfp319	C	A	8: 96,055,410 (GRCm39)	K264N	probably damaging	Het
Zfp62	C	T	11: 49,107,580 (GRCm39)	S557L	probably damaging	Het
Zgrf1	T	C	3: 127,357,239 (GRCm39)	S822P	probably damaging	Het
Zxdc	A	G	6: 90,346,645 (GRCm39)	D2G	possibly damaging	Het
Zzef1	T	C	11: 72,742,727 (GRCm39)	I769T	possibly damaging	Het

Other mutations in Fam91a1

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00090:Fam91a1	APN	15	58,302,584 (GRCm39)	missense	probably damaging	0.99
IGL00590:Fam91a1	APN	15	58,287,565 (GRCm39)	missense	possibly damaging	0.66
IGL01301:Fam91a1	APN	15	58,314,720 (GRCm39)	missense	probably damaging	0.99
IGL01372:Fam91a1	APN	15	58,301,911 (GRCm39)	missense	probably damaging	1.00
IGL01979:Fam91a1	APN	15	58,304,433 (GRCm39)	missense	probably damaging	1.00
IGL02085:Fam91a1	APN	15	58,313,505 (GRCm39)	missense	possibly damaging	0.95
IGL02553:Fam91a1	APN	15	58,304,831 (GRCm39)	critical splice donor site	probably null
IGL02605:Fam91a1	APN	15	58,303,045 (GRCm39)	splice site	probably benign
IGL02882:Fam91a1	APN	15	58,324,910 (GRCm39)	splice site	probably benign
IGL02894:Fam91a1	APN	15	58,315,080 (GRCm39)	missense	probably benign	0.09
ANU18:Fam91a1	UTSW	15	58,314,720 (GRCm39)	missense	probably damaging	0.99
H8562:Fam91a1	UTSW	15	58,298,970 (GRCm39)	splice site	probably null
R0395:Fam91a1	UTSW	15	58,326,641 (GRCm39)	missense	probably benign
R1165:Fam91a1	UTSW	15	58,302,518 (GRCm39)	missense	possibly damaging	0.90
R1699:Fam91a1	UTSW	15	58,304,797 (GRCm39)	missense	probably benign	0.04
R1749:Fam91a1	UTSW	15	58,298,443 (GRCm39)	missense	probably benign	0.02
R1997:Fam91a1	UTSW	15	58,296,044 (GRCm39)	critical splice acceptor site	probably null
R2042:Fam91a1	UTSW	15	58,298,443 (GRCm39)	missense	probably benign	0.02
R2188:Fam91a1	UTSW	15	58,302,512 (GRCm39)	missense	probably damaging	0.98
R2518:Fam91a1	UTSW	15	58,322,449 (GRCm39)	missense	possibly damaging	0.92
R3124:Fam91a1	UTSW	15	58,293,738 (GRCm39)	missense	probably benign	0.34
R3916:Fam91a1	UTSW	15	58,302,583 (GRCm39)	missense	probably damaging	1.00
R4810:Fam91a1	UTSW	15	58,306,589 (GRCm39)	missense	probably damaging	1.00
R4959:Fam91a1	UTSW	15	58,303,059 (GRCm39)	missense	probably benign	0.16
R4973:Fam91a1	UTSW	15	58,303,059 (GRCm39)	missense	probably benign	0.16
R5288:Fam91a1	UTSW	15	58,320,243 (GRCm39)	missense	probably benign
R5385:Fam91a1	UTSW	15	58,320,243 (GRCm39)	missense	probably benign
R5386:Fam91a1	UTSW	15	58,320,243 (GRCm39)	missense	probably benign
R5941:Fam91a1	UTSW	15	58,303,166 (GRCm39)	missense	probably benign	0.01
R6415:Fam91a1	UTSW	15	58,314,766 (GRCm39)	missense	probably damaging	1.00
R6869:Fam91a1	UTSW	15	58,303,117 (GRCm39)	missense	probably benign	0.00
R7872:Fam91a1	UTSW	15	58,320,209 (GRCm39)	missense	probably benign	0.01
X0024:Fam91a1	UTSW	15	58,302,038 (GRCm39)	missense	probably damaging	0.98
Z1177:Fam91a1	UTSW	15	58,304,397 (GRCm39)	missense	possibly damaging	0.68

Predicted Primers

PCR Primer
(F):5'- ACTCCAGCCTCCTTAGTAGAGG -3'
(R):5'- GGATTCACCTCAATTGGCAGAG -3'

Sequencing Primer
(F):5'- CAGCCTCCTTAGTAGAGGATCTAG -3'
(R):5'- GGAAACTGTCATAAAGGACA -3'

Posted On

2019-06-26