Mutagenetix > Incidental Mutation

Incidental Mutation 'R7183:Olfr835'

559115

Institutional Source

Beutler Lab

Gene Symbol

Olfr835

Ensembl Gene

ENSMUSG00000045204

Gene Name

olfactory receptor 835

Synonyms

MOR150-3, GA_x6K02T2PVTD-12771995-12772930

MMRRC Submission

045235-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.131) question?

Stock #

R7183 (G1)

Quality Score

225.009

Status

Validated

Chromosome

Chromosomal Location

19032735-19037670 bp(+) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

G to T at 19035332 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Aspartic acid to Tyrosine at position 70 (D70Y)

Ref Sequence

ENSEMBL: ENSMUSP00000148416 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000059315] [ENSMUST00000213018]

AlphaFold

Q7TRG7

Predicted Effect

probably damaging
Transcript: ENSMUST00000059315
AA Change: D70Y

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)

SMART Domains

Protein: ENSMUSP00000052610
Gene: ENSMUSG00000045204
AA Change: D70Y

Domain	Start	End	E-Value	Type
Pfam:7tm_4	31	308	1.8e-47	PFAM
Pfam:7TM_GPCR_Srsx	35	206	2.9e-7	PFAM
Pfam:7tm_1	41	303	1.3e-23	PFAM

Predicted Effect

probably damaging
Transcript: ENSMUST00000213018
AA Change: D70Y

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)

Coding Region Coverage

1x: 100.0%
3x: 100.0%
10x: 99.7%
20x: 99.2%

Validation Efficiency

100% (65/65)

MGI Phenotype

FUNCTION: Olfactory receptors interact with odorant molecules in the nose, to initiate a neuronal response that triggers the perception of a smell. The olfactory receptor proteins are members of a large family of G-protein-coupled receptors (GPCR) arising from single coding-exon genes. Olfactory receptors share a 7-transmembrane domain structure with many neurotransmitter and hormone receptors and are responsible for the recognition and G protein-mediated transduction of odorant signals. The olfactory receptor gene family is the largest in the genome. The nomenclature assigned to the olfactory receptor genes and proteins for this organism is independent of other organisms. [provided by RefSeq, Jul 2008]

Allele List at MGI

Other mutations in this stock

Total: 64 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
4931408C20Rik	G	A	1: 26,682,833	L1089F	probably benign	Het
Actn1	T	A	12: 80,168,932	M816L	possibly damaging	Het
Ahnak	T	C	19: 9,017,668	F5439L	probably damaging	Het
Apba2	G	A	7: 64,733,545	D369N	probably benign	Het
Arhgap32	A	G	9: 32,186,383	N228D	probably benign	Het
Arhgap33	T	A	7: 30,525,871		probably null	Het
Cacna1g	C	T	11: 94,439,737	C984Y	probably benign	Het
Cadm2	C	A	16: 66,882,832	G47*	probably null	Het
Ccdc125	A	G	13: 100,690,358	D241G	possibly damaging	Het
Ccdc39	T	G	3: 33,814,471	E822A	probably damaging	Het
Cd86	CA	CAA	16: 36,606,555		probably null	Het
Cdc42bpg	A	G	19: 6,310,797	D195G	probably damaging	Het
Cdkl1	T	C	12: 69,748,932	R275G	probably damaging	Het
Chst4	T	C	8: 110,029,998	N411S	possibly damaging	Het
Cir1	A	G	2: 73,286,386	V210A	probably damaging	Het
Col6a1	A	G	10: 76,716,259		probably null	Het
Crmp1	C	A	5: 37,288,817	H606N	probably benign	Het
Cyp2j8	A	T	4: 96,479,181	N233K	probably damaging	Het
Dennd1b	A	T	1: 139,170,252	Q677L	unknown	Het
Dnah17	G	A	11: 118,129,188	T11I	probably benign	Het
Ehd1	A	G	19: 6,297,654	H346R	probably benign	Het
Emc3	G	T	6: 113,531,384	Y33*	probably null	Het
Ercc5	A	T	1: 44,161,808		probably null	Het
Ercc5	G	T	1: 44,161,809		probably null	Het
Fat3	A	C	9: 15,922,837	I4153S	possibly damaging	Het
Fn3krp	T	C	11: 121,421,605		probably null	Het
Gm2035	G	A	12: 87,919,722	R46W	possibly damaging	Het
Gmnc	C	T	16: 26,960,529	D249N	probably benign	Het
Gsn	C	T	2: 35,294,948	A305V	probably benign	Het
Haus6	A	T	4: 86,583,752	H627Q	possibly damaging	Het
Heg1	A	G	16: 33,738,550		probably null	Het
Hoxd9	G	T	2: 74,698,365	V104L	possibly damaging	Het
Igkv10-96	A	C	6: 68,632,216	S32A	probably benign	Het
Kcnd2	G	A	6: 21,216,437	V47M	probably damaging	Het
Mab21l3	C	T	3: 101,815,153	V386M	probably damaging	Het
Masp2	A	G	4: 148,612,157	S404G	probably benign	Het
Olfr1024	T	A	2: 85,904,142	Q304L	probably benign	Het
Olfr1454	A	G	19: 13,064,316	I302V	probably benign	Het
P4htm	A	T	9: 108,581,860	M291K	possibly damaging	Het
Pde6c	T	C	19: 38,133,090	S49P	probably benign	Het
Pdzd7	A	G	19: 45,037,114	V314A	probably benign	Het
Pfkl	G	A	10: 78,002,082	R31*	probably null	Het
Phlpp2	C	A	8: 109,939,953	P1038Q	probably damaging	Het
Pik3c2b	T	C	1: 133,066,465	S56P	probably benign	Het
Plec	A	G	15: 76,205,705	V145A	unknown	Het
Prg3	G	T	2: 84,993,023	D181Y	probably damaging	Het
Prg3	G	A	2: 84,991,504	V158I	probably benign	Het
Rbp3	A	G	14: 33,955,204	T370A	probably benign	Het
Rgl2	T	C	17: 33,934,990	F457L	possibly damaging	Het
Rubcnl	T	A	14: 75,049,626	M578K	probably damaging	Het
Siae	G	A	9: 37,616,946	V72M	possibly damaging	Het
Smchd1	A	T	17: 71,353,516	D1864E	probably benign	Het
Smox	T	C	2: 131,520,566	I255T	possibly damaging	Het
Tas2r123	A	G	6: 132,847,698	N186S	possibly damaging	Het
Thbs2	T	A	17: 14,690,116	I74F	possibly damaging	Het
Timm44	T	C	8: 4,267,311	D238G	probably damaging	Het
Tlk2	T	C	11: 105,221,359		probably null	Het
Tnc	A	G	4: 64,013,128	S782P	probably damaging	Het
Tpr	A	T	1: 150,406,551	K336N	probably damaging	Het
Uggt2	A	T	14: 119,019,637		probably null	Het
Vmn2r101	T	A	17: 19,612,178	I812N	probably damaging	Het
Vps33a	T	C	5: 123,535,215	Q436R	probably null	Het
Ywhaq	T	C	12: 21,416,869	K75E	possibly damaging	Het
Zfp87	A	G	13: 67,517,474	S290P	probably damaging	Het

Other mutations in Olfr835

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01718:Olfr835	APN	9	19035288	nonsense	probably null
IGL02546:Olfr835	APN	9	19035354	missense	possibly damaging	0.88
R0010:Olfr835	UTSW	9	19035322	missense	probably damaging	1.00
R0107:Olfr835	UTSW	9	19035333	missense	probably damaging	1.00
R1867:Olfr835	UTSW	9	19035266	missense	probably benign	0.36
R1891:Olfr835	UTSW	9	19035978	missense	probably damaging	0.99
R1941:Olfr835	UTSW	9	19035866	missense	possibly damaging	0.93
R4152:Olfr835	UTSW	9	19035520	nonsense	probably null
R5451:Olfr835	UTSW	9	19035491	missense	probably damaging	1.00
R5677:Olfr835	UTSW	9	19035558	missense	possibly damaging	0.93
R5970:Olfr835	UTSW	9	19035147	missense	probably benign
R6187:Olfr835	UTSW	9	19035393	missense	probably benign	0.12
R6805:Olfr835	UTSW	9	19035301	missense	probably damaging	1.00
R8029:Olfr835	UTSW	9	19035794	missense	probably damaging	1.00
R8139:Olfr835	UTSW	9	19035575	missense	probably benign	0.07
R8736:Olfr835	UTSW	9	19035478	missense	probably damaging	1.00
R8868:Olfr835	UTSW	9	19035482	missense	probably damaging	1.00
R8909:Olfr835	UTSW	9	19035592	missense	probably benign	0.08
R9116:Olfr835	UTSW	9	19035477	missense	probably damaging	1.00
R9479:Olfr835	UTSW	9	19035434	missense	probably benign	0.30
R9786:Olfr835	UTSW	9	19035945	missense	possibly damaging	0.87

Predicted Primers

PCR Primer
(F):5'- TCTTCTGGGACTAACAGATGATC -3'
(R):5'- CCCATGTTCAAAACGCTAATGAG -3'

Sequencing Primer
(F):5'- ATCCAACTGTGAAGCCTGTG -3'
(R):5'- TCATCATGACTGTATAACTCAGTGG -3'

Posted On

2019-06-26