Mutagenetix > Incidental Mutations

Incidental Mutation 'R7191:Zfp277'

559611

Institutional Source

Beutler Lab

Gene Symbol

Zfp277

Ensembl Gene

ENSMUSG00000055917

Gene Name

zinc finger protein 277

Synonyms

2410017E24Rik, NIRF4

MMRRC Submission

Accession Numbers

Is this an essential gene?

Probably non essential (E-score: 0.074) question?

Stock #

R7191 (G1)

Quality Score

225.009

Status

Validated

Chromosome

Chromosomal Location

40315046-40445902 bp(-) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

A to T at 40329562 bp

Zygosity

Heterozygous

Amino Acid Change

Histidine to Glutamine at position 324 (H324Q)

Ref Sequence

ENSEMBL: ENSMUSP00000064226 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000069637] [ENSMUST00000069692]

Predicted Effect

probably damaging
Transcript: ENSMUST00000069637
AA Change: H198Q

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)

SMART Domains

Protein: ENSMUSP00000068032
Gene: ENSMUSG00000055917
AA Change: H198Q

Domain	Start	End	E-Value	Type
ZnF_C2H2	59	84	4.27e1	SMART
coiled coil region	143	171	N/A	INTRINSIC
ZnF_C2H2	174	198	3.85e1	SMART
ZnF_C2H2	225	249	2.24e-3	SMART
low complexity region	280	292	N/A	INTRINSIC
ZnF_C2H2	303	326	1.91e1	SMART
ZnF_C2H2	356	382	4.94e0	SMART

Predicted Effect

probably damaging
Transcript: ENSMUST00000069692
AA Change: H324Q

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)

SMART Domains

Protein: ENSMUSP00000064226
Gene: ENSMUSG00000055917
AA Change: H324Q

Domain	Start	End	E-Value	Type
ZnF_C2H2	185	210	4.27e1	SMART
coiled coil region	269	297	N/A	INTRINSIC
ZnF_C2H2	300	324	3.85e1	SMART
ZnF_C2H2	351	375	2.24e-3	SMART
low complexity region	406	418	N/A	INTRINSIC
ZnF_C2H2	429	452	1.91e1	SMART
ZnF_C2H2	482	508	4.94e0	SMART

Meta Mutation Damage Score

0.9574

Coding Region Coverage

1x: 100.0%
3x: 100.0%
10x: 99.8%
20x: 99.2%

Validation Efficiency

100% (52/52)

MGI Phenotype

PHENOTYPE: Mice homozygous for a knock-out allele exhibit early cellular preplicative senescence in MEFs. [provided by MGI curators]

Allele List at MGI

Other mutations in this stock

Total: 52 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
2510009E07Rik	T	A	16: 21,653,564	I129F	probably benign	Het
Ank2	T	A	3: 126,946,392	T1948S	unknown	Het
Arap1	T	C	7: 101,384,992	C214R	probably benign	Het
BC024978	G	A	7: 27,201,123	A176T	probably damaging	Het
Ccdc82	C	T	9: 13,252,472	Q130*	probably null	Het
Cfh	A	C	1: 140,112,567	V597G	probably benign	Het
Clp1	A	T	2: 84,724,146	C226*	probably null	Het
Cyp39a1	G	A	17: 43,731,019	W372*	probably null	Het
Dchs1	G	A	7: 105,765,439	P799S	possibly damaging	Het
Dmbt1	A	T	7: 131,044,520	N167I	unknown	Het
Dock10	A	G	1: 80,540,331	S1310P	possibly damaging	Het
Fam83h	C	T	15: 76,003,037	G817D	probably damaging	Het
Fras1	A	G	5: 96,614,912	T758A	probably benign	Het
Fryl	T	C	5: 73,072,912	H1634R	probably damaging	Het
Gcg	C	G	2: 62,476,839	G126A	probably damaging	Het
Gm4763	C	T	7: 24,723,334	V105I	possibly damaging	Het
Gm4951	A	T	18: 60,246,257	D288V	probably benign	Het
Gne	T	C	4: 44,040,266	K633E	probably benign	Het
Gpr4	T	C	7: 19,223,230	V359A	probably benign	Het
Gprc5c	C	T	11: 114,868,617	T422M	possibly damaging	Het
Gria2	C	A	3: 80,732,085	V207L	probably benign	Het
Hid1	T	C	11: 115,348,469	*789W	probably null	Het
Jakmip3	G	A	7: 138,989,528		probably null	Het
Kin	G	A	2: 10,091,793	R151Q	probably benign	Het
Krt16	T	C	11: 100,246,658	E407G	probably damaging	Het
Krt81	T	C	15: 101,460,229	D381G	probably damaging	Het
Lrrc34	C	T	3: 30,624,878	G357S	possibly damaging	Het
Mipol1	A	G	12: 57,457,066	Q340R	probably benign	Het
Mpzl3	T	G	9: 45,055,244	M1R	probably null	Het
Nrcam	A	G	12: 44,572,244	N852S	probably benign	Het
Nudcd2	C	T	11: 40,736,603	Q117*	probably null	Het
Nup35	A	T	2: 80,658,379	E320V	probably damaging	Het
Olfml3	T	C	3: 103,735,860	K402E	probably damaging	Het
Olfr477	G	A	7: 107,990,646	V94M	possibly damaging	Het
Pkhd1	T	C	1: 20,558,719	H668R	probably benign	Het
Ptprc	T	A	1: 138,101,044	D333V	probably benign	Het
Rdh16	A	G	10: 127,813,418	K248E	probably benign	Het
Samd4b	C	A	7: 28,414,261	G93V	probably benign	Het
Scgn	A	G	13: 23,981,493	I78T	probably benign	Het
Slfn14	T	C	11: 83,276,749	I647V	probably benign	Het
Smyd5	T	C	6: 85,440,111	V157A	probably benign	Het
Syde2	T	C	3: 146,002,358	M951T	probably benign	Het
Tas2r105	T	C	6: 131,686,982	N161S	probably damaging	Het
Tbck	T	C	3: 132,737,555	F581L	probably damaging	Het
Traip	A	G	9: 107,970,017	N352D	probably benign	Het
Trim29	A	G	9: 43,311,609	Y245C	probably damaging	Het
Trpv4	T	C	5: 114,633,140	I443V	probably benign	Het
Usp36	C	T	11: 118,268,834	E595K	probably benign	Het
Vmn2r51	T	A	7: 10,100,553	Y186F	probably null	Het
Vmn2r97	A	G	17: 18,930,286	Y465C	probably damaging	Het
Wrb	A	G	16: 96,151,945	I79V	possibly damaging	Het
Zfp987	A	G	4: 146,121,903	D17G	probably damaging	Het

Other mutations in Zfp277

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01466:Zfp277	APN	12	40378826	missense	probably benign	0.24
IGL01477:Zfp277	APN	12	40320676	missense	probably benign	0.00
IGL02081:Zfp277	APN	12	40328796	nonsense	probably null
IGL02165:Zfp277	APN	12	40315803	missense	possibly damaging	0.75
IGL02613:Zfp277	APN	12	40329515	missense	probably damaging	1.00
IGL02688:Zfp277	APN	12	40328688	missense	possibly damaging	0.95
IGL02825:Zfp277	APN	12	40317176	missense	probably benign	0.06
R0194:Zfp277	UTSW	12	40378877	splice site	probably benign
R0226:Zfp277	UTSW	12	40364162	missense	possibly damaging	0.67
R0843:Zfp277	UTSW	12	40320600	critical splice donor site	probably null
R1263:Zfp277	UTSW	12	40364165	missense	probably damaging	0.99
R1584:Zfp277	UTSW	12	40378826	missense	probably benign	0.12
R1609:Zfp277	UTSW	12	40328720	missense	probably damaging	0.99
R1644:Zfp277	UTSW	12	40329610	splice site	probably null
R1789:Zfp277	UTSW	12	40364085	missense	probably benign	0.00
R1882:Zfp277	UTSW	12	40445746	missense	probably benign	0.03
R2011:Zfp277	UTSW	12	40317218	nonsense	probably null
R4884:Zfp277	UTSW	12	40363153	missense	probably damaging	0.97
R4976:Zfp277	UTSW	12	40328688	missense	possibly damaging	0.95
R5119:Zfp277	UTSW	12	40328688	missense	possibly damaging	0.95
R5532:Zfp277	UTSW	12	40335309	missense	probably damaging	1.00
R6340:Zfp277	UTSW	12	40318549	missense	possibly damaging	0.57
R7378:Zfp277	UTSW	12	40315853	missense	possibly damaging	0.94
R7446:Zfp277	UTSW	12	40328730	missense	probably damaging	1.00
R7564:Zfp277	UTSW	12	40329595	missense	probably damaging	0.99
R7861:Zfp277	UTSW	12	40315881	missense	possibly damaging	0.92
R8428:Zfp277	UTSW	12	40329578	missense	probably damaging	1.00

Predicted Primers

PCR Primer
(F):5'- TGGTCAATGTTTGGCATAAAGC -3'
(R):5'- CATAGCCATCTTAGCGAGGCTC -3'

Sequencing Primer
(F):5'- AGGGTAAAATCCCACAGT -3'
(R):5'- CATCTTAGCGAGGCTCAAGTG -3'

Posted On

2019-06-26