Mutagenetix > Incidental Mutation

Incidental Mutation 'R7195:Zfp933'

559845

Institutional Source

Beutler Lab

Gene Symbol

Zfp933

Ensembl Gene

ENSMUSG00000059423

Gene Name

zinc finger protein 933

Synonyms

2810408P10Rik

MMRRC Submission

Accession Numbers

Is this an essential gene?

Probably non essential (E-score: 0.057) question?

Stock #

R7195 (G1)

Quality Score

225.009

Status

Not validated

Chromosome

Chromosomal Location

147822986-147848366 bp(-) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

G to A at 147826179 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Threonine to Isoleucine at position 320 (T320I)

Ref Sequence

ENSEMBL: ENSMUSP00000101343 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000105718] [ENSMUST00000135798]

AlphaFold

Q6PEE4

Predicted Effect

probably benign
Transcript: ENSMUST00000105718
AA Change: T320I

PolyPhen 2 Score 0.158 (Sensitivity: 0.92; Specificity: 0.87)

SMART Domains

Protein: ENSMUSP00000101343
Gene: ENSMUSG00000059423
AA Change: T320I

Domain	Start	End	E-Value	Type
KRAB	4	66	9.49e-16	SMART
ZnF_C2H2	131	153	3.21e-4	SMART
ZnF_C2H2	159	181	5.21e-4	SMART
ZnF_C2H2	187	209	2.12e-4	SMART
ZnF_C2H2	215	237	7.26e-3	SMART
ZnF_C2H2	243	265	6.88e-4	SMART
ZnF_C2H2	271	293	1.13e-4	SMART
ZnF_C2H2	299	321	3.95e-4	SMART
ZnF_C2H2	327	349	1.56e-2	SMART
ZnF_C2H2	355	377	1.79e-2	SMART
ZnF_C2H2	383	405	4.24e-4	SMART
ZnF_C2H2	411	433	1.22e-4	SMART
ZnF_C2H2	439	461	4.79e-3	SMART

Predicted Effect

probably benign
Transcript: ENSMUST00000135798

SMART Domains

Protein: ENSMUSP00000118300
Gene: ENSMUSG00000059423

Domain	Start	End	E-Value	Type
Blast:KRAB	1	34	8e-18	BLAST
PDB:2I13\|B	32	98	1e-12	PDB
SCOP:d1fgja_	33	98	5e-13	SMART
Blast:PHD	44	98	6e-11	BLAST

Coding Region Coverage

1x: 100.0%
3x: 99.9%
10x: 99.7%
20x: 98.8%

Validation Efficiency

Allele List at MGI

Other mutations in this stock

Total: 64 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
4930438A08Rik	T	C	11: 58,288,416		probably null	Het
4930563D23Rik	C	T	16: 92,321,149	V84M	probably damaging	Het
Abca2	A	G	2: 25,442,076	D1400G	probably benign	Het
Actl11	A	T	9: 107,928,870	K131*	probably null	Het
Adam39	A	T	8: 40,824,775	R68*	probably null	Het
Akap7	A	T	10: 25,271,507	N108K	probably damaging	Het
Arhgef10l	C	A	4: 140,611,410	A14S	probably benign	Het
BC067074	A	T	13: 113,367,929	D1864V		Het
Ccdc141	T	G	2: 77,049,583	N632T	probably benign	Het
Ccm2	T	C	11: 6,596,302	S435P	probably damaging	Het
Cd36	A	G	5: 17,814,189	L178P	probably damaging	Het
Cd7	T	C	11: 121,038,249	I59V	probably benign	Het
Cds2	T	A	2: 132,293,284	S32T	probably benign	Het
Cep19	A	G	16: 32,107,086	D104G	probably damaging	Het
Col1a2	C	T	6: 4,510,753	P68S	unknown	Het
Col20a1	A	G	2: 181,007,231	H1011R	probably damaging	Het
D7Ertd443e	A	G	7: 134,295,122	V513A	probably damaging	Het
Egfr	C	T	11: 16,868,162	P228L	probably damaging	Het
Fam78b	A	G	1: 167,078,562	R97G	probably damaging	Het
Flt1	C	T	5: 147,603,576	V768M	probably damaging	Het
Gdap1l1	A	T	2: 163,446,130	N96Y	probably damaging	Het
Gm7168	T	A	17: 13,949,360	Y330N	probably benign	Het
Hnrnpul1	T	C	7: 25,724,778	N683S	unknown	Het
Ice2	C	T	9: 69,428,500	P922S	possibly damaging	Het
Iglv2	A	G	16: 19,260,510	V81A	not run	Het
Irs1	A	G	1: 82,287,456	I1013T	probably benign	Het
Itih4	T	C	14: 30,899,475	S832P	probably damaging	Het
Klhl1	A	T	14: 96,280,077	Y388N	probably benign	Het
Lrrc37a	G	A	11: 103,457,775	S2698L	unknown	Het
Map4k4	T	C	1: 40,019,669	Y1008H	possibly damaging	Het
Mdn1	G	T	4: 32,701,823	G1519W	probably damaging	Het
Mga	T	A	2: 119,917,328	D653E	probably damaging	Het
Npas1	T	C	7: 16,474,808	E48G	probably damaging	Het
Nup188	G	A	2: 30,341,830		probably null	Het
Olfr1215	G	A	2: 89,001,731	L186F		Het
Olfr33	A	G	7: 102,713,666	V249A	possibly damaging	Het
Olfr399	T	A	11: 74,054,397	M121L	probably damaging	Het
Olfr404-ps1	G	T	11: 74,239,568	M1I	probably null	Het
Olfr544	A	T	7: 102,484,367	V251D	probably damaging	Het
Olfr871	A	G	9: 20,212,544	N65S	probably damaging	Het
Oxct1	T	C	15: 4,128,901	V439A	probably damaging	Het
Pcdhb22	G	T	18: 37,519,288	G13W	probably damaging	Het
Pex11g	A	G	8: 3,459,237	V230A	probably benign	Het
Pop1	C	T	15: 34,510,379	S439L	probably damaging	Het
Ptpn9	T	A	9: 57,022,249	H83Q	probably benign	Het
Qrfp	C	T	2: 31,808,692	R76H	probably benign	Het
Slc30a3	A	G	5: 31,088,795	V197A	probably benign	Het
Slc8a3	T	C	12: 81,314,273	N591D	possibly damaging	Het
Sp4	G	T	12: 118,300,072	Q80K	possibly damaging	Het
Spock1	C	T	13: 57,907,502	G29D	possibly damaging	Het
Sprr1a	G	T	3: 92,484,367	P109Q	probably damaging	Het
Suz12	T	A	11: 80,013,483	F239L	probably damaging	Het
Tbx3	A	G	5: 119,675,583	Y248C	probably damaging	Het
Trabd2b	T	C	4: 114,409,440	L217P	probably damaging	Het
Ubxn11	A	C	4: 134,126,415	I398L	possibly damaging	Het
Vmn1r173	T	A	7: 23,702,459	S40T	probably damaging	Het
Vmn2r2	T	C	3: 64,116,479	S894G	probably benign	Het
Vmn2r65	A	G	7: 84,943,139		probably null	Het
Vps13c	G	A	9: 67,945,825	G2400D	possibly damaging	Het
Vps8	T	C	16: 21,456,282	Y197H	probably damaging	Het
Wdr91	T	C	6: 34,889,274	N486D	possibly damaging	Het
Wwp1	A	G	4: 19,627,908	I695T	possibly damaging	Het
Zc3hc1	T	C	6: 30,382,548	D133G	probably benign	Het
Zcchc14	T	C	8: 121,608,461	I307V	unknown	Het

Other mutations in Zfp933

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00576:Zfp933	APN	4	147826321	missense	probably damaging	1.00
IGL03377:Zfp933	APN	4	147828711	missense	possibly damaging	0.65
F5770:Zfp933	UTSW	4	147826470	missense	probably damaging	0.98
FR4340:Zfp933	UTSW	4	147825729	frame shift	probably null
FR4548:Zfp933	UTSW	4	147825731	frame shift	probably null
R0388:Zfp933	UTSW	4	147826442	missense	probably benign	0.35
R0523:Zfp933	UTSW	4	147826462	nonsense	probably null
R0539:Zfp933	UTSW	4	147826548	missense	probably benign	0.08
R1672:Zfp933	UTSW	4	147826019	missense	probably damaging	1.00
R4049:Zfp933	UTSW	4	147826512	missense	probably damaging	1.00
R4895:Zfp933	UTSW	4	147826435	nonsense	probably null
R5133:Zfp933	UTSW	4	147826864	missense	probably benign
R5786:Zfp933	UTSW	4	147828407	splice site	probably null
R5891:Zfp933	UTSW	4	147826774	missense	probably benign	0.03
R6111:Zfp933	UTSW	4	147828760	missense	probably damaging	1.00
R6382:Zfp933	UTSW	4	147825868	missense	probably benign	0.07
R6968:Zfp933	UTSW	4	147826197	missense	probably damaging	1.00
R7555:Zfp933	UTSW	4	147826132	missense	probably damaging	1.00
R7902:Zfp933	UTSW	4	147826601	missense	probably damaging	0.96
R8319:Zfp933	UTSW	4	147828453	missense	possibly damaging	0.87
R8688:Zfp933	UTSW	4	147826792	missense	probably benign	0.14
R9019:Zfp933	UTSW	4	147826564	missense	probably damaging	1.00
RF024:Zfp933	UTSW	4	147826441	missense	probably damaging	1.00
RF028:Zfp933	UTSW	4	147825731	frame shift	probably null
RF035:Zfp933	UTSW	4	147825731	makesense	probably null
RF043:Zfp933	UTSW	4	147825731	frame shift	probably null
V7581:Zfp933	UTSW	4	147826470	missense	probably damaging	0.98
V7582:Zfp933	UTSW	4	147826470	missense	probably damaging	0.98
V7583:Zfp933	UTSW	4	147826470	missense	probably damaging	0.98

Predicted Primers

PCR Primer
(F):5'- TGTTTTGTGAAAAGGCTGAACCA -3'
(R):5'- TTGCGCAAAATGGTCATCTCATAATA -3'

Sequencing Primer
(F):5'- CTGCAAAGGCTTTACCACATTG -3'
(R):5'- TACACAAAAGAACACATACCGGAGAG -3'

Posted On

2019-06-26