Incidental Mutation 'R7197:Gpr158'
| ID |
559985 |
| Institutional Source |
Beutler Lab
|
| Gene Symbol |
Gpr158
|
| Ensembl Gene |
ENSMUSG00000045967 |
| Gene Name |
G protein-coupled receptor 158 |
| Synonyms |
5330427M13Rik |
| MMRRC Submission |
045276-MU
|
| Accession Numbers |
|
| Essential gene? |
Non essential
(E-score: 0.000)
|
| Stock # |
R7197 (G1)
|
| Quality Score |
225.009 |
|
Status
|
Validated
|
| Chromosome |
2 |
| Chromosomal Location |
21372378-21835355 bp(+) (GRCm39) |
| Type of Mutation |
missense |
| DNA Base Change (assembly) |
A to T
at 21815412 bp (GRCm39)
|
| Zygosity |
Heterozygous |
| Amino Acid Change |
Threonine to Serine
at position 602
(T602S)
|
| Ref Sequence |
ENSEMBL: ENSMUSP00000049708
(fasta)
|
| Gene Model |
predicted gene model for transcript(s):
[ENSMUST00000055946]
|
| AlphaFold |
Q8C419 |
| Predicted Effect |
probably damaging
Transcript: ENSMUST00000055946
AA Change: T602S
PolyPhen 2
Score 0.989 (Sensitivity: 0.72; Specificity: 0.97)
|
| SMART Domains |
Protein: ENSMUSP00000049708
Gene: ENSMUSG00000045967
AA Change: T602S
| Domain | Start | End | E-Value | Type |
|---|
|
signal peptide
|
1 |
20 |
N/A |
INTRINSIC |
|
low complexity region
|
110 |
125 |
N/A |
INTRINSIC |
|
SCOP:d1edmb_
|
313 |
359 |
5e-4 |
SMART |
|
Blast:EGF
|
318 |
365 |
2e-27 |
BLAST |
|
Pfam:7tm_3
|
426 |
669 |
1.2e-35 |
PFAM |
|
low complexity region
|
840 |
863 |
N/A |
INTRINSIC |
|
| Meta Mutation Damage Score |
0.0679 |
| Coding Region Coverage |
- 1x: 100.0%
- 3x: 100.0%
- 10x: 99.8%
- 20x: 99.3%
|
| Validation Efficiency |
97% (77/79) |
| Allele List at MGI |
|
| Other mutations in this stock |
Total: 77 list
| Gene | Ref | Var | Chr/Loc | Mutation | Predicted Effect | Zygosity |
|---|
| 1700017B05Rik |
AGCTTCCCTGCTT |
AGCTT |
9: 57,165,505 (GRCm39) |
|
probably null |
Het |
| Aars2 |
T |
C |
17: 45,819,885 (GRCm39) |
Y221H |
probably damaging |
Het |
| Abca8b |
G |
A |
11: 109,836,648 (GRCm39) |
R1216* |
probably null |
Het |
| Acad8 |
A |
T |
9: 26,888,967 (GRCm39) |
|
probably null |
Het |
| Acer2 |
A |
G |
4: 86,805,287 (GRCm39) |
T84A |
probably null |
Het |
| Adgrb2 |
A |
G |
4: 129,903,315 (GRCm39) |
N613S |
probably damaging |
Het |
| Arap2 |
T |
C |
5: 62,798,729 (GRCm39) |
D1300G |
possibly damaging |
Het |
| Castor1 |
T |
C |
11: 4,168,949 (GRCm39) |
C39R |
probably damaging |
Het |
| Ccl11 |
A |
T |
11: 81,948,665 (GRCm39) |
|
probably benign |
Het |
| Cfh |
A |
T |
1: 140,016,505 (GRCm39) |
Y1151* |
probably null |
Het |
| Cobll1 |
A |
G |
2: 64,928,882 (GRCm39) |
S815P |
probably benign |
Het |
| Cryz |
T |
C |
3: 154,327,205 (GRCm39) |
S240P |
probably damaging |
Het |
| Csmd2 |
A |
G |
4: 128,404,826 (GRCm39) |
Y2404C |
|
Het |
| F13a1 |
T |
C |
13: 37,100,860 (GRCm39) |
D428G |
probably damaging |
Het |
| Fbxo42 |
A |
G |
4: 140,927,396 (GRCm39) |
S559G |
probably benign |
Het |
| Fry |
A |
G |
5: 150,393,232 (GRCm39) |
S263G |
|
Het |
| Fshr |
C |
T |
17: 89,292,897 (GRCm39) |
A594T |
probably damaging |
Het |
| Gm1527 |
A |
G |
3: 28,980,690 (GRCm39) |
M597V |
probably null |
Het |
| Hmgxb4 |
C |
A |
8: 75,756,624 (GRCm39) |
T583K |
probably damaging |
Het |
| Igkv8-30 |
C |
T |
6: 70,094,069 (GRCm39) |
C114Y |
probably damaging |
Het |
| Igsf5 |
T |
A |
16: 96,204,546 (GRCm39) |
N366K |
probably damaging |
Het |
| Kif15 |
T |
C |
9: 122,838,991 (GRCm39) |
|
probably null |
Het |
| Klk1b26 |
T |
A |
7: 43,665,821 (GRCm39) |
|
probably null |
Het |
| Lama1 |
G |
T |
17: 68,044,700 (GRCm39) |
E200* |
probably null |
Het |
| Lpar5 |
C |
T |
6: 125,059,347 (GRCm39) |
T356I |
probably benign |
Het |
| Lrch3 |
A |
T |
16: 32,810,665 (GRCm39) |
T538S |
probably damaging |
Het |
| Mansc4 |
A |
G |
6: 146,977,203 (GRCm39) |
S138P |
probably damaging |
Het |
| Mief1 |
T |
C |
15: 80,134,061 (GRCm39) |
S373P |
possibly damaging |
Het |
| Nbas |
G |
T |
12: 13,570,751 (GRCm39) |
V2053F |
possibly damaging |
Het |
| Nek11 |
T |
C |
9: 105,121,614 (GRCm39) |
E435G |
probably damaging |
Het |
| Nynrin |
A |
T |
14: 56,109,380 (GRCm39) |
I1496F |
probably benign |
Het |
| Odf1 |
A |
T |
15: 38,219,794 (GRCm39) |
M41L |
probably benign |
Het |
| Or10a5 |
A |
G |
7: 106,635,364 (GRCm39) |
M8V |
probably benign |
Het |
| Or4c115 |
A |
G |
2: 88,927,626 (GRCm39) |
V215A |
probably benign |
Het |
| Or4c29 |
A |
T |
2: 88,740,708 (GRCm39) |
F10I |
probably benign |
Het |
| Or7h8 |
T |
G |
9: 20,123,851 (GRCm39) |
S69A |
probably benign |
Het |
| Oxnad1 |
T |
C |
14: 31,818,473 (GRCm39) |
V106A |
possibly damaging |
Het |
| Padi4 |
C |
A |
4: 140,488,969 (GRCm39) |
E157* |
probably null |
Het |
| Parp10 |
A |
T |
15: 76,126,616 (GRCm39) |
F217L |
probably damaging |
Het |
| Pcdhb18 |
T |
A |
18: 37,623,436 (GRCm39) |
S255R |
probably benign |
Het |
| Pde4c |
G |
T |
8: 71,197,978 (GRCm39) |
G102W |
probably damaging |
Het |
| Pde6a |
T |
C |
18: 61,391,295 (GRCm39) |
Y547H |
probably damaging |
Het |
| Phf11b |
A |
T |
14: 59,563,507 (GRCm39) |
L137I |
probably benign |
Het |
| Pigh |
A |
C |
12: 79,130,463 (GRCm39) |
I134S |
probably damaging |
Het |
| Pigt |
G |
A |
2: 164,344,436 (GRCm39) |
V362M |
probably damaging |
Het |
| Pld1 |
A |
G |
3: 28,078,401 (GRCm39) |
D20G |
probably damaging |
Het |
| Pou3f2 |
T |
C |
4: 22,487,288 (GRCm39) |
R282G |
probably damaging |
Het |
| Ppp2r5d |
A |
G |
17: 46,996,527 (GRCm39) |
V382A |
probably damaging |
Het |
| Prdm16 |
T |
C |
4: 154,425,967 (GRCm39) |
E606G |
probably damaging |
Het |
| Psg28 |
A |
G |
7: 18,164,509 (GRCm39) |
Y68H |
probably damaging |
Het |
| Qpctl |
C |
A |
7: 18,882,944 (GRCm39) |
W56L |
probably damaging |
Het |
| Rab11fip5 |
G |
A |
6: 85,319,137 (GRCm39) |
P584L |
probably damaging |
Het |
| Sde2 |
C |
T |
1: 180,678,843 (GRCm39) |
H36Y |
probably benign |
Het |
| Sema4d |
T |
C |
13: 51,856,872 (GRCm39) |
S787G |
probably benign |
Het |
| Sgca |
C |
T |
11: 94,864,014 (GRCm39) |
|
probably null |
Het |
| Shroom3 |
A |
G |
5: 93,090,463 (GRCm39) |
E1071G |
probably damaging |
Het |
| Skint5 |
A |
G |
4: 113,437,679 (GRCm39) |
|
probably null |
Het |
| Slc44a3 |
T |
A |
3: 121,319,411 (GRCm39) |
D110V |
probably benign |
Het |
| Slc4a4 |
A |
C |
5: 89,082,433 (GRCm39) |
|
probably benign |
Het |
| Slc4a8 |
A |
G |
15: 100,688,857 (GRCm39) |
D389G |
probably damaging |
Het |
| Sp3 |
C |
G |
2: 72,809,953 (GRCm39) |
E11Q |
probably benign |
Het |
| Srrm2 |
A |
G |
17: 24,037,198 (GRCm39) |
S1281G |
unknown |
Het |
| Tat |
A |
T |
8: 110,723,459 (GRCm39) |
I316F |
probably benign |
Het |
| Thoc5 |
A |
G |
11: 4,865,563 (GRCm39) |
T381A |
probably benign |
Het |
| Tiam1 |
T |
C |
16: 89,681,826 (GRCm39) |
Y384C |
probably damaging |
Het |
| Tmem45a |
A |
T |
16: 56,632,026 (GRCm39) |
F197L |
probably damaging |
Het |
| Trav8d-1 |
A |
G |
14: 53,016,435 (GRCm39) |
Y107C |
probably damaging |
Het |
| Trim10 |
T |
A |
17: 37,187,846 (GRCm39) |
V354D |
probably damaging |
Het |
| Unc45b |
A |
T |
11: 82,831,013 (GRCm39) |
|
probably null |
Het |
| Unc79 |
A |
G |
12: 103,078,765 (GRCm39) |
I1643M |
probably benign |
Het |
| Unc80 |
G |
T |
1: 66,560,725 (GRCm39) |
G818* |
probably null |
Het |
| Usp15 |
A |
T |
10: 122,966,910 (GRCm39) |
M470K |
possibly damaging |
Het |
| Vldlr |
T |
C |
19: 27,212,241 (GRCm39) |
V85A |
probably benign |
Het |
| Vma21-ps |
A |
G |
4: 52,496,994 (GRCm39) |
V84A |
probably benign |
Het |
| Vmn2r67 |
C |
T |
7: 84,785,774 (GRCm39) |
V744I |
possibly damaging |
Het |
| Wdr73 |
A |
G |
7: 80,542,946 (GRCm39) |
C221R |
probably benign |
Het |
| Zxdc |
C |
T |
6: 90,355,819 (GRCm39) |
H443Y |
probably damaging |
Het |
|
| Other mutations in Gpr158 |
| Allele | Source | Chr | Coord | Type | Predicted Effect | PPH Score |
|---|
|
IGL00340:Gpr158
|
APN |
2 |
21,373,494 (GRCm39) |
missense |
probably damaging |
1.00 |
|
IGL00469:Gpr158
|
APN |
2 |
21,751,606 (GRCm39) |
splice site |
probably benign |
|
|
IGL00706:Gpr158
|
APN |
2 |
21,751,584 (GRCm39) |
missense |
probably damaging |
1.00 |
|
IGL00780:Gpr158
|
APN |
2 |
21,831,629 (GRCm39) |
nonsense |
probably null |
|
|
IGL00885:Gpr158
|
APN |
2 |
21,653,832 (GRCm39) |
missense |
probably damaging |
1.00 |
|
IGL01339:Gpr158
|
APN |
2 |
21,373,842 (GRCm39) |
missense |
possibly damaging |
0.73 |
|
IGL01368:Gpr158
|
APN |
2 |
21,831,909 (GRCm39) |
missense |
probably damaging |
1.00 |
|
IGL02141:Gpr158
|
APN |
2 |
21,788,101 (GRCm39) |
missense |
probably damaging |
0.99 |
|
IGL02455:Gpr158
|
APN |
2 |
21,373,511 (GRCm39) |
missense |
probably benign |
0.00 |
|
IGL02554:Gpr158
|
APN |
2 |
21,831,407 (GRCm39) |
missense |
probably benign |
|
|
IGL02681:Gpr158
|
APN |
2 |
21,820,441 (GRCm39) |
missense |
probably damaging |
1.00 |
|
IGL02752:Gpr158
|
APN |
2 |
21,831,638 (GRCm39) |
missense |
possibly damaging |
0.95 |
|
IGL02756:Gpr158
|
APN |
2 |
21,831,890 (GRCm39) |
missense |
possibly damaging |
0.47 |
|
IGL03181:Gpr158
|
APN |
2 |
21,787,972 (GRCm39) |
missense |
probably benign |
0.02 |
|
IGL03258:Gpr158
|
APN |
2 |
21,830,085 (GRCm39) |
missense |
probably damaging |
1.00 |
|
IGL03386:Gpr158
|
APN |
2 |
21,831,057 (GRCm39) |
missense |
probably damaging |
1.00 |
|
PIT4810001:Gpr158
|
UTSW |
2 |
21,831,682 (GRCm39) |
missense |
probably benign |
0.01 |
|
R0071:Gpr158
|
UTSW |
2 |
21,815,479 (GRCm39) |
missense |
probably benign |
0.08 |
|
R0081:Gpr158
|
UTSW |
2 |
21,831,528 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R0528:Gpr158
|
UTSW |
2 |
21,830,019 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R0560:Gpr158
|
UTSW |
2 |
21,830,085 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R0603:Gpr158
|
UTSW |
2 |
21,820,480 (GRCm39) |
missense |
possibly damaging |
0.67 |
|
R1560:Gpr158
|
UTSW |
2 |
21,831,125 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R1561:Gpr158
|
UTSW |
2 |
21,820,505 (GRCm39) |
splice site |
probably null |
|
|
R1609:Gpr158
|
UTSW |
2 |
21,788,104 (GRCm39) |
missense |
possibly damaging |
0.61 |
|
R1741:Gpr158
|
UTSW |
2 |
21,832,359 (GRCm39) |
missense |
probably benign |
0.00 |
|
R1827:Gpr158
|
UTSW |
2 |
21,832,129 (GRCm39) |
missense |
probably benign |
|
|
R1854:Gpr158
|
UTSW |
2 |
21,373,935 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R1871:Gpr158
|
UTSW |
2 |
21,820,426 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R2151:Gpr158
|
UTSW |
2 |
21,832,325 (GRCm39) |
missense |
possibly damaging |
0.82 |
|
R2273:Gpr158
|
UTSW |
2 |
21,831,674 (GRCm39) |
missense |
probably benign |
|
|
R2275:Gpr158
|
UTSW |
2 |
21,831,674 (GRCm39) |
missense |
probably benign |
|
|
R3004:Gpr158
|
UTSW |
2 |
21,831,810 (GRCm39) |
missense |
probably damaging |
0.99 |
|
R3151:Gpr158
|
UTSW |
2 |
21,581,771 (GRCm39) |
missense |
possibly damaging |
0.68 |
|
R3943:Gpr158
|
UTSW |
2 |
21,373,370 (GRCm39) |
missense |
possibly damaging |
0.65 |
|
R4238:Gpr158
|
UTSW |
2 |
21,373,362 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R4379:Gpr158
|
UTSW |
2 |
21,830,025 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R4381:Gpr158
|
UTSW |
2 |
21,832,403 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R4464:Gpr158
|
UTSW |
2 |
21,831,810 (GRCm39) |
missense |
probably damaging |
0.99 |
|
R4467:Gpr158
|
UTSW |
2 |
21,831,810 (GRCm39) |
missense |
probably damaging |
0.99 |
|
R4496:Gpr158
|
UTSW |
2 |
21,831,810 (GRCm39) |
missense |
probably damaging |
0.99 |
|
R4506:Gpr158
|
UTSW |
2 |
21,831,810 (GRCm39) |
missense |
probably damaging |
0.99 |
|
R4530:Gpr158
|
UTSW |
2 |
21,373,811 (GRCm39) |
missense |
probably benign |
0.03 |
|
R4646:Gpr158
|
UTSW |
2 |
21,831,864 (GRCm39) |
missense |
probably benign |
|
|
R4798:Gpr158
|
UTSW |
2 |
21,787,993 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R4882:Gpr158
|
UTSW |
2 |
21,830,059 (GRCm39) |
missense |
probably damaging |
0.98 |
|
R4943:Gpr158
|
UTSW |
2 |
21,831,968 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R5334:Gpr158
|
UTSW |
2 |
21,832,316 (GRCm39) |
missense |
probably benign |
0.01 |
|
R5560:Gpr158
|
UTSW |
2 |
21,831,101 (GRCm39) |
missense |
possibly damaging |
0.67 |
|
R5600:Gpr158
|
UTSW |
2 |
21,832,046 (GRCm39) |
missense |
probably benign |
|
|
R5637:Gpr158
|
UTSW |
2 |
21,788,083 (GRCm39) |
missense |
probably benign |
0.00 |
|
R5701:Gpr158
|
UTSW |
2 |
21,751,520 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R5744:Gpr158
|
UTSW |
2 |
21,373,331 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R5911:Gpr158
|
UTSW |
2 |
21,373,932 (GRCm39) |
missense |
possibly damaging |
0.95 |
|
R5991:Gpr158
|
UTSW |
2 |
21,373,319 (GRCm39) |
missense |
probably damaging |
0.99 |
|
R6200:Gpr158
|
UTSW |
2 |
21,404,227 (GRCm39) |
missense |
probably damaging |
0.97 |
|
R6306:Gpr158
|
UTSW |
2 |
21,820,422 (GRCm39) |
missense |
possibly damaging |
0.84 |
|
R6324:Gpr158
|
UTSW |
2 |
21,815,365 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R6384:Gpr158
|
UTSW |
2 |
21,831,099 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R6698:Gpr158
|
UTSW |
2 |
21,831,921 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R6997:Gpr158
|
UTSW |
2 |
21,653,802 (GRCm39) |
missense |
possibly damaging |
0.46 |
|
R7086:Gpr158
|
UTSW |
2 |
21,831,386 (GRCm39) |
missense |
probably benign |
0.01 |
|
R7175:Gpr158
|
UTSW |
2 |
21,373,113 (GRCm39) |
missense |
probably benign |
0.13 |
|
R7293:Gpr158
|
UTSW |
2 |
21,581,750 (GRCm39) |
missense |
possibly damaging |
0.47 |
|
R7427:Gpr158
|
UTSW |
2 |
21,832,129 (GRCm39) |
missense |
probably benign |
|
|
R7515:Gpr158
|
UTSW |
2 |
21,373,092 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R7730:Gpr158
|
UTSW |
2 |
21,831,158 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R8122:Gpr158
|
UTSW |
2 |
21,831,674 (GRCm39) |
missense |
probably benign |
|
|
R8311:Gpr158
|
UTSW |
2 |
21,373,701 (GRCm39) |
missense |
probably benign |
0.00 |
|
R8754:Gpr158
|
UTSW |
2 |
21,581,693 (GRCm39) |
missense |
probably benign |
0.00 |
|
R8782:Gpr158
|
UTSW |
2 |
21,404,149 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R8792:Gpr158
|
UTSW |
2 |
21,558,137 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R8842:Gpr158
|
UTSW |
2 |
21,581,751 (GRCm39) |
missense |
possibly damaging |
0.88 |
|
R9009:Gpr158
|
UTSW |
2 |
21,581,760 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R9102:Gpr158
|
UTSW |
2 |
21,830,078 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R9150:Gpr158
|
UTSW |
2 |
21,831,251 (GRCm39) |
missense |
probably benign |
0.17 |
|
R9254:Gpr158
|
UTSW |
2 |
21,373,042 (GRCm39) |
start gained |
probably benign |
|
|
R9317:Gpr158
|
UTSW |
2 |
21,832,037 (GRCm39) |
missense |
probably benign |
|
|
R9379:Gpr158
|
UTSW |
2 |
21,373,042 (GRCm39) |
start gained |
probably benign |
|
|
R9428:Gpr158
|
UTSW |
2 |
21,787,972 (GRCm39) |
missense |
probably benign |
|
|
R9497:Gpr158
|
UTSW |
2 |
21,831,825 (GRCm39) |
missense |
probably benign |
0.00 |
|
R9667:Gpr158
|
UTSW |
2 |
21,830,054 (GRCm39) |
missense |
probably damaging |
0.99 |
|
R9681:Gpr158
|
UTSW |
2 |
21,831,315 (GRCm39) |
missense |
probably damaging |
0.99 |
|
X0062:Gpr158
|
UTSW |
2 |
21,831,180 (GRCm39) |
missense |
probably damaging |
1.00 |
|
Z1176:Gpr158
|
UTSW |
2 |
21,815,501 (GRCm39) |
critical splice donor site |
probably null |
|
|
Z1177:Gpr158
|
UTSW |
2 |
21,832,083 (GRCm39) |
missense |
possibly damaging |
0.46 |
|
| Predicted Primers |
PCR Primer
(F):5'- TGTGTTGTAAGCAATGGCAGC -3'
(R):5'- GCCACAATCAGGTATTCATAAAGC -3'
Sequencing Primer
(F):5'- TTGTAAGCAATGGCAGCAGCAG -3'
(R):5'- TTGTACCACTGCCAAGGA -3'
|
| Posted On |
2019-06-26 |
Incidental Mutation