Mutagenetix > Incidental Mutation

Incidental Mutation 'R7202:Adgrf3'

560361

Institutional Source

Beutler Lab

Gene Symbol

Adgrf3

Ensembl Gene

ENSMUSG00000067642

Gene Name

adhesion G protein-coupled receptor F3

Synonyms

Gpr113, LOC381628, PGR23

MMRRC Submission

045280-MU

Accession Numbers

Essential gene?

Non essential (E-score: 0.000) question?

Stock #

R7202 (G1)

Quality Score

225.009

Status

Validated

Chromosome

Chromosomal Location

30193431-30205722 bp(-) (GRCm38)

Type of Mutation

nonsense

DNA Base Change (assembly)

T to A at 30204380 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Lysine to Stop codon at position 38 (K38*)

Ref Sequence

ENSEMBL: ENSMUSP00000085440 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000088117]

AlphaFold

Q58Y75

Predicted Effect

probably null
Transcript: ENSMUST00000088117
AA Change: K38*

SMART Domains

Protein: ENSMUSP00000085440
Gene: ENSMUSG00000067642
AA Change: K38*

Domain	Start	End	E-Value	Type
signal peptide	1	20	N/A	INTRINSIC
low complexity region	85	97	N/A	INTRINSIC
Blast:IG	163	252	2e-20	BLAST
Blast:CCP	341	399	1e-6	BLAST
low complexity region	403	415	N/A	INTRINSIC
low complexity region	471	483	N/A	INTRINSIC
GPS	632	684	2.68e-17	SMART
Pfam:7tm_2	687	935	1e-30	PFAM

Meta Mutation Damage Score

0.9755

Coding Region Coverage

1x: 100.0%
3x: 100.0%
10x: 99.7%
20x: 99.0%

Validation Efficiency

99% (74/75)

Allele List at MGI

Other mutations in this stock

Total: 74 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Abca14	T	C	7: 120,318,013 (GRCm38)	I1467T	probably damaging	Het
Ahdc1	T	A	4: 133,061,887 (GRCm38)	C146*	probably null	Het
Ahnak	A	T	19: 9,017,799 (GRCm38)	K5482N	probably damaging	Het
AI464131	T	C	4: 41,498,268 (GRCm38)	Y454C	probably damaging	Het
Arhgap23	C	T	11: 97,451,993 (GRCm38)	A367V	possibly damaging	Het
Arvcf	T	C	16: 18,405,198 (GRCm38)	S960P	probably damaging	Het
Asb14	C	A	14: 26,900,437 (GRCm38)	P74Q	probably benign	Het
Atad5	T	C	11: 80,089,775 (GRCm38)	V2A	probably damaging	Het
Baz2a	A	G	10: 128,118,559 (GRCm38)	I691M	possibly damaging	Het
Brca2	A	G	5: 150,532,354 (GRCm38)	T187A	probably benign	Het
Btbd11	A	G	10: 85,387,765 (GRCm38)	E146G	unknown	Het
Calr4	T	A	4: 109,244,057 (GRCm38)	N152K	possibly damaging	Het
Ccnb2	C	T	9: 70,410,846 (GRCm38)	A215T	probably damaging	Het
Cdh16	C	T	8: 104,614,148 (GRCm38)	D140N	unknown	Het
Ces1g	T	C	8: 93,302,967 (GRCm38)	T546A	probably benign	Het
Cfap74	G	T	4: 155,426,197 (GRCm38)		probably null	Het
Col5a1	G	T	2: 27,952,378 (GRCm38)	D422Y	unknown	Het
Cyp2c66	A	T	19: 39,141,904 (GRCm38)	Y61F	probably damaging	Het
Dnah12	G	A	14: 26,778,912 (GRCm38)		probably null	Het
Dnah6	A	G	6: 73,181,705 (GRCm38)		probably null	Het
Dopey1	T	G	9: 86,504,167 (GRCm38)		probably null	Het
Fam163a	A	G	1: 156,079,088 (GRCm38)	F106L	probably damaging	Het
Gfy	T	C	7: 45,178,172 (GRCm38)	T167A	probably benign	Het
Gm6525	A	T	3: 84,175,034 (GRCm38)	R87S	probably benign	Het
Gramd1c	C	A	16: 44,059,221 (GRCm38)	A19S	possibly damaging	Het
Herc2	T	C	7: 56,131,286 (GRCm38)	V1233A	probably damaging	Het
Hibch	G	A	1: 52,853,715 (GRCm38)		probably null	Het
Ibsp	A	T	5: 104,302,161 (GRCm38)	S9C	probably benign	Het
Il17ra	A	G	6: 120,475,611 (GRCm38)	I252V	probably benign	Het
Ino80	A	T	2: 119,374,437 (GRCm38)	M1536K	probably benign	Het
Ipo11	A	T	13: 106,875,570 (GRCm38)	L568Q	probably damaging	Het
Kcnu1	T	G	8: 25,919,581 (GRCm38)		probably null	Het
Kdm5b	T	G	1: 134,624,759 (GRCm38)	S1158A	probably benign	Het
Kmt2a	T	C	9: 44,847,715 (GRCm38)	T946A	probably benign	Het
Kmt2e	A	T	5: 23,492,294 (GRCm38)		probably benign	Het
Krba1	G	A	6: 48,412,327 (GRCm38)	V594M	probably damaging	Het
Lamb1	T	C	12: 31,324,315 (GRCm38)	L1559P	probably damaging	Het
Lig1	C	T	7: 13,291,249 (GRCm38)	P227S	probably benign	Het
Lims2	T	A	18: 31,956,964 (GRCm38)	D255E	probably benign	Het
Maats1	T	C	16: 38,335,597 (GRCm38)	Y88C	probably benign	Het
March3	C	T	18: 56,776,089 (GRCm38)	C208Y	probably benign	Het
Mei1	A	G	15: 82,092,642 (GRCm38)	E142G		Het
Melk	G	T	4: 44,351,106 (GRCm38)	R549L	probably benign	Het
Muc6	AGGCGCAGAAACCCTGGC	AGGC	7: 141,634,450 (GRCm38)		probably null	Het
Nae1	T	C	8: 104,523,583 (GRCm38)	Y226C	possibly damaging	Het
Nap1l1	T	C	10: 111,491,103 (GRCm38)	F175S	probably damaging	Het
Ndn	T	C	7: 62,348,961 (GRCm38)	L185P	probably damaging	Het
Ndst3	T	A	3: 123,671,739 (GRCm38)	I195F	possibly damaging	Het
Ndufaf1	A	T	2: 119,658,426 (GRCm38)	S206T	probably benign	Het
Nek10	C	A	14: 14,836,171 (GRCm38)	H131N	probably benign	Het
Obsl1	T	A	1: 75,489,716 (GRCm38)	D1456V	possibly damaging	Het
Olfr1328	C	T	4: 118,934,018 (GRCm38)	V277I	probably benign	Het
Olfr605	T	A	7: 103,443,085 (GRCm38)	T13S	probably benign	Het
Olfr714	A	G	7: 107,074,241 (GRCm38)	N138D	probably benign	Het
Otog	C	T	7: 46,288,050 (GRCm38)	L1728F	probably damaging	Het
Pcx	A	T	19: 4,602,333 (GRCm38)	M150L	possibly damaging	Het
Pole	A	G	5: 110,297,107 (GRCm38)	D443G	possibly damaging	Het
Prr14	G	T	7: 127,476,476 (GRCm38)	R552L	probably damaging	Het
Rasgrf1	T	G	9: 90,017,072 (GRCm38)	S1156A	possibly damaging	Het
Rfc4	T	A	16: 23,127,609 (GRCm38)		probably benign	Het
Rfx5	G	T	3: 94,958,961 (GRCm38)	A524S	unknown	Het
Ryr3	G	A	2: 112,766,319 (GRCm38)	P2497S	probably damaging	Het
Scg3	T	C	9: 75,683,710 (GRCm38)	T6A	probably benign	Het
Slc26a9	A	T	1: 131,762,788 (GRCm38)	D510V	possibly damaging	Het
Stpg3	A	G	2: 25,214,574 (GRCm38)	F10L	probably damaging	Het
Tmc5	T	A	7: 118,639,956 (GRCm38)	H357Q	possibly damaging	Het
Tmem40	A	T	6: 115,730,459 (GRCm38)	L253H	probably damaging	Het
Tmie	A	G	9: 110,867,564 (GRCm38)	V83A	probably damaging	Het
Uck2	A	T	1: 167,226,515 (GRCm38)	I218N	probably damaging	Het
Vtn	A	G	11: 78,500,800 (GRCm38)	D310G	possibly damaging	Het
Wdr49	A	T	3: 75,333,273 (GRCm38)	S430T	probably benign	Het
Wtip	A	G	7: 34,132,662 (GRCm38)	V133A	probably benign	Het
Zbtb9	T	A	17: 26,974,692 (GRCm38)	V357E	probably damaging	Het
Zfp953	G	A	13: 67,343,642 (GRCm38)	T82M	probably benign	Het

Other mutations in Adgrf3

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL03080:Adgrf3	APN	5	30,196,829 (GRCm38)	missense	probably benign	0.02
IGL03171:Adgrf3	APN	5	30,196,294 (GRCm38)	missense	probably damaging	1.00
R0010:Adgrf3	UTSW	5	30,205,609 (GRCm38)	splice site	probably benign
R0042:Adgrf3	UTSW	5	30,197,428 (GRCm38)	missense	probably damaging	1.00
R0140:Adgrf3	UTSW	5	30,196,381 (GRCm38)	missense	probably benign	0.19
R0617:Adgrf3	UTSW	5	30,195,080 (GRCm38)	missense	probably benign	0.25
R0748:Adgrf3	UTSW	5	30,196,876 (GRCm38)	missense	probably damaging	1.00
R1291:Adgrf3	UTSW	5	30,199,534 (GRCm38)	missense	probably damaging	0.99
R1330:Adgrf3	UTSW	5	30,195,095 (GRCm38)	missense	probably benign	0.24
R1468:Adgrf3	UTSW	5	30,202,229 (GRCm38)	splice site	probably benign
R1695:Adgrf3	UTSW	5	30,203,555 (GRCm38)	missense	probably benign	0.05
R1716:Adgrf3	UTSW	5	30,197,551 (GRCm38)	missense	probably benign	0.03
R1844:Adgrf3	UTSW	5	30,199,213 (GRCm38)	missense	probably damaging	0.96
R1935:Adgrf3	UTSW	5	30,202,306 (GRCm38)	missense	probably benign	0.00
R1936:Adgrf3	UTSW	5	30,202,306 (GRCm38)	missense	probably benign	0.00
R2059:Adgrf3	UTSW	5	30,199,491 (GRCm38)	missense	possibly damaging	0.91
R2656:Adgrf3	UTSW	5	30,196,438 (GRCm38)	missense	possibly damaging	0.96
R2913:Adgrf3	UTSW	5	30,196,994 (GRCm38)	missense	probably damaging	1.00
R2914:Adgrf3	UTSW	5	30,196,994 (GRCm38)	missense	probably damaging	1.00
R2987:Adgrf3	UTSW	5	30,197,360 (GRCm38)	missense	probably damaging	1.00
R3797:Adgrf3	UTSW	5	30,196,823 (GRCm38)	missense	possibly damaging	0.49
R3798:Adgrf3	UTSW	5	30,196,823 (GRCm38)	missense	possibly damaging	0.49
R3799:Adgrf3	UTSW	5	30,196,823 (GRCm38)	missense	possibly damaging	0.49
R3934:Adgrf3	UTSW	5	30,200,434 (GRCm38)	unclassified	probably benign
R4043:Adgrf3	UTSW	5	30,204,362 (GRCm38)	missense	probably benign	0.00
R4080:Adgrf3	UTSW	5	30,197,369 (GRCm38)	nonsense	probably null
R4575:Adgrf3	UTSW	5	30,202,257 (GRCm38)	missense	probably benign	0.00
R4754:Adgrf3	UTSW	5	30,197,617 (GRCm38)	critical splice acceptor site	probably null
R4819:Adgrf3	UTSW	5	30,198,444 (GRCm38)	missense	possibly damaging	0.66
R4893:Adgrf3	UTSW	5	30,200,478 (GRCm38)	missense	probably benign	0.00
R4991:Adgrf3	UTSW	5	30,199,148 (GRCm38)	missense	probably benign	0.26
R5686:Adgrf3	UTSW	5	30,197,306 (GRCm38)	missense	probably damaging	1.00
R5965:Adgrf3	UTSW	5	30,205,639 (GRCm38)	missense	probably benign	0.00
R5997:Adgrf3	UTSW	5	30,198,362 (GRCm38)	critical splice donor site	probably null
R6103:Adgrf3	UTSW	5	30,196,267 (GRCm38)	missense	probably damaging	1.00
R6244:Adgrf3	UTSW	5	30,197,533 (GRCm38)	missense	probably benign	0.17
R6409:Adgrf3	UTSW	5	30,197,314 (GRCm38)	missense	probably damaging	0.96
R6575:Adgrf3	UTSW	5	30,196,524 (GRCm38)	missense	possibly damaging	0.72
R6745:Adgrf3	UTSW	5	30,203,603 (GRCm38)	missense	probably benign	0.31
R6790:Adgrf3	UTSW	5	30,196,387 (GRCm38)	missense	probably benign	0.00
R6813:Adgrf3	UTSW	5	30,197,521 (GRCm38)	missense	probably damaging	0.96
R7250:Adgrf3	UTSW	5	30,195,682 (GRCm38)	missense	probably damaging	1.00
R7353:Adgrf3	UTSW	5	30,198,497 (GRCm38)	missense	probably damaging	0.98
R7634:Adgrf3	UTSW	5	30,202,247 (GRCm38)	missense	probably benign	0.01
R7658:Adgrf3	UTSW	5	30,197,206 (GRCm38)	missense	probably benign	0.41
R8037:Adgrf3	UTSW	5	30,199,512 (GRCm38)	missense	probably damaging	1.00
R8281:Adgrf3	UTSW	5	30,197,303 (GRCm38)	missense	possibly damaging	0.46
R8717:Adgrf3	UTSW	5	30,198,581 (GRCm38)	unclassified	probably benign
R8857:Adgrf3	UTSW	5	30,197,067 (GRCm38)	nonsense	probably null
R8926:Adgrf3	UTSW	5	30,200,448 (GRCm38)	missense	possibly damaging	0.46
R9391:Adgrf3	UTSW	5	30,195,073 (GRCm38)	missense	possibly damaging	0.94
R9446:Adgrf3	UTSW	5	30,196,959 (GRCm38)	missense	probably benign	0.01
R9522:Adgrf3	UTSW	5	30,199,484 (GRCm38)	missense	possibly damaging	0.90
Z1088:Adgrf3	UTSW	5	30,199,120 (GRCm38)	missense	possibly damaging	0.90

Predicted Primers

PCR Primer
(F):5'- GTCTTTCTAGGCCTCACAGC -3'
(R):5'- CCTGGAATTGTGCCCCTGTC -3'

Sequencing Primer
(F):5'- CCTGGAGCTCAGTTTATAGACCAG -3'
(R):5'- GAACATCCTTTTCTCCCTCCAGATAC -3'

Posted On

2019-06-26